Oral granular cell tumor: a collaborative clinicopathological study of 61 cases.

BACKGROUND: Granular Cell Tumor (GCT) is an uncommon benign lesion in the oral cavity whose pathogenesis remains poorly understood. Due to their infrequent occurrence and similarity to other oral lesions, they are often forgotten during the initial clinical diagnosis. Therefore, understanding its prevalence, clinical and pathological characteristics is crucial for an accurate diagnosis and adequate management. METHODS: All cases diagnosed as GCTs in six Brazilian and Argentinian oral diagnostic centers were re-evaluated by HE staining, and clinical, demographic, and histopathological data were collected and evaluated. RESULTS: The series comprised 45 female (73.8%) and 16 male (26.2%), with a 2.8:1 female-to-male ratio and a mean age of 35.3 ± 16.9 years (range: 7-77 years). Most cases occurred on the tongue (n = 49; 81.6%) and presented clinically as asymptomatic papules or nodules (n = 50; 89.3%) with a normochromic (n = 25; 45.5%) or yellowish (n = 11; 20.0%) coloration and sizes ranging from 0.2 to 3.0 cm (mean ± SD: 1.40 ± 0.75 cm). Morphologically, most tumors were characterized by a poorly delimited proliferation (n = 52; 88.1%) of cells typically rounded to polygonal containing abundant, eosinophilic, finely granular cytoplasm. Pustulo-ovoid bodies of Milian were identified in all lesions (n = 61; 100%). Entrapment of skeletal striated muscle (n = 44; 72.1%) and nerve fibers (n = 42; 68.9%) were common findings. Pseudoepitheliomatous hyperplasia (PEH) was observed in 23 cases (39.0%). In only 27.7% of cases (n = 13) there was agreement between the clinical and histopathological diagnosis. Outcome information was available from 16 patients (26.2%), with clinical follow-up ranging from 4 to 36 months (mean 13.3 months), and none developed local recurrence. CONCLUSIONS: The clinical and histopathological features of GCTs were consistent with those described in previous studies. In general, these lesions have a predilection for the lateral region of the tongue in adult women. It is essential to consider GCTs in the differential diagnosis of yellow or normochromic papules and nodules in the oral cavity. Histopathological evaluation is essential for the definitive diagnosis and the prognosis is excellent.

Association of IGF-1 and IGF-2 genotypes with respiratory muscle strength in individuals with COPD: A cross-sectional study / Asociación de los genotipos de IGF-1 e IGF-2 con la fuerza muscular respiratoria en individuos con EPOC: estudio transversal

Introduction Chronic obstructive pulmonary disease is a systemic disease characterized not only by respiratory symptoms but also by physical deconditioning and muscle weakness. One prominent manifestation of this disease is the decline in respiratory muscle strength. Previous studies have linked the genotypes of insulin-like growth factor 1 and 2 (IGF-1 and IGF-2) to muscle weakness in other populations without this disease. However, there is a notable knowledge gap regarding the biological mechanisms underlying respiratory muscle weakness, particularly the role of IGF-1 and IGF-2 genotypes in this pulmonary disease. Therefore, this study aimed to investigate, for the first time, the association between IGF-1 and IGF-2 genotypes with respiratory muscle strength in individuals with chronic obstructive pulmonary disease. In addition, we analyzed the relationship between oxidative stress, chronic inflammation, and vitamin D with respiratory muscle strength. Methods A cross sectional study with 61 individuals with chronic obstructive pulmonary disease. Polymerase chain reaction of gene polymorphisms IGF-1 (rs35767) and IGF-2 (rs3213221) was analyzed. Other variables, related to oxidative stress, inflammation and Vitamin D were dosed from peripheral blood. Maximal inspiratory and expiratory pressure were measured. Results The genetic polymorphisms were associated with respiratory muscle strength ( 3.0 and 3.5; = 0.57). Specific genotypes of IGF-1 and IGF-2 presented lower maximal inspiratory and expiratory pressure (<0.05 for all). Oxidative stress, inflammatory biomarkers, and vitamin D were not associated with respiratory muscle strength. Conclusion The polymorphisms of IGF-1 and IGF-2 displayed stronger correlations with respiratory muscle strength compared to blood biomarkers in patients with chronic obstructive pulmonary disease. Specific genotypes of IGF-1 and IGF-2 were associated with reduced respiratory muscle strength in this population.

Introducción La enfermedad pulmonar obstructiva crónica es una enfermedad sistémica caracterizada no solo por síntomas respiratorios, sino también por el deterioro físico y la debilidad muscular. Una manifestación destacada de esta enfermedad es el declive en la fuerza de los músculos respiratorios. Estudios previos han vinculado los genotipos de factor de crecimiento insulínico 1 y 2 (IGF-1 e IGF-2) con la debilidad muscular en poblaciones sin esta enfermedad. Sin embargo, existe un vacío de conocimiento con respecto a los mecanismos biológicos subyacentes a la debilidad de los músculos respiratorios, en particular el papel de los genotipos IGF-1 e IGF-2 en esta enfermedad pulmonar. Por lo tanto, este estudio tuvo como objetivo investigar, por primera vez, la asociación de los genotipos IGF-1 e IGF-2 con la fuerza de los músculos respiratorios en individuos con enfermedad pulmonar obstructiva crónica. Además, analizamos la relación entre el estrés oxidativo, la inflamación crónica y la vitamina D con la fuerza de los músculos respiratorios. Métodos Un estudio transversal con 61 individuos con enfermedad pulmonar obstructiva crónica. Se analizó la reacción en cadena de la polimerasa de los polimorfismos genéticos IGF-1 (rs35767) e IGF-2 (rs3213221). Otras variables relacionadas con el estrés oxidativo, la inflamación y la vitamina D se dosificaron a partir de muestras de sangre periférica. Se midieron las presiones inspiratorias y espiratorias máximas. Resultados Los polimorfismos genéticos están asociados con la fuerza de los músculos respiratorios (F: 3.0 y 3.5; R2= 0.57). Genotipos específicos de IGF-1 e IGF-2 presentaron bajos valores en las presiones inspiratorias y espiratorias (p<0.05 en todos los casos). El estrés oxidativo, los biomarcadores inflamatorios y la vitamina D no se asociaron con la fuerza de los músculos respiratorios. Conclusión Los polimorfismos de IGF-1 e IGF-2 mostraron correlaciones más sólidas con la fuerza de los músculos respiratorios en pacientes con enfermedad pulmonar obstructiva crónica en comparación con los biomarcadores sanguíneos. Genotipos específicos de IGF-1 e IGF-2 se asociaron con una disminución de la fuerza de los músculos respiratorios en esta población

Aspectos ultraestruturais de células Sirc infectadas pelo vírus zika em diferentes períodos de infecção / Ultrastructural aspects of Sirc cells infected by the zika virus at different periods of infection

O Zika vírus (ZIKV) pertence à família Flaviviridae, gênero Flavivírus, é um vírus envelopado, icosaédrico, medindo de 40 nm a 60 nm de diâmetro, possui genoma constituído de ácido ribonucleíco de fita simples, senso positivo (ssRNA(+)). É transmitido pela picada de artrópodes hematófagos da espécie Aedes. O ZIKV foi descoberto acidentalmente em 1947, durante um estudo sobre a Febre Amarela, na floresta Zika, em Uganda (África). Após alguns relatos de transmissão na África, espalhou-se para outros países, afetando milhares de pessoas. Em 2015, observou-se no Brasil um aumento significativo de bebês nascidos com microcefalia em áreas onde o ZIKV foi notificado meses antes. Alguns pacientes adultos desenvolveram a síndrome de Guillain-Barré ou alterações oculares na retina e na coroide. O objetivo deste estudo foi investigar os aspectos ultraestruturais de células SIRC infectadas com o vírus Zika, em diferentes períodos de infecção, a fim de compreender a possível relação entre a infecção pelo ZIKV e a desordem ocular. Células SIRC (epitelial corneal Rabbit Cell - ATCC CL 60) foram infectadas com ZIKV (inóculo armazenado na coleção de vírus, do Centro de Virologia, do Instituto Adolfo Lutz, sob o número BR310161) e processadas após 24hs, 48hs, 72hs, 96hs e 9 dias pela técnica de Microscopia Eletrônica de Transmissão (MET). Nossos resultados mostraram alterações em organelas celulares como: mitocôndrias com matriz elétron densas e/ou alterações nas cristas, Complexo de Golgi com cisternas dilatadas liberando vesículas com ou sem partículas virais, Retículo Endoplasmático dilatado, estruturas tubulares...(AU)

The Zika virus (ZIKV) belongs to the family Flaviviridae, genus Flavivirus, is an enveloped, icosahedral virus, measuring from 40 nm to 60 nm in diameter, has a genome consisting of ribonucleic acid of simple tape, positive sense (ssRNA(+). It is transmitted by the bite of hematophagous arthropods of the species Aedes. ZIKV was accidentally discovered in 1947, during a study on Yellow Fever in the Zika forest in Uganda (Africa). After some reports of transmission in Africa, it spread to other countries, affecting thousands of people. In 2015, in Brazil, there was a significant increase in babies born with microcephaly in areas where ZIKV was reported months earlier. Some adult patients developed Guillain-Barré syndrome, eye changes, specifically in the retina and choroid. The aim of this study was to investigate the ultrastructural aspects of SIRC cells infected with Zika virus at different periods of infection in order to understand the possible relationship between ZIKV infection and eye disorder. SIRC cells (corneal epithelial Rabbit Cell - ATCC CL 60) were infected with ZIKV (stored in the virus collection of the Virology Center of the Adolfo Lutz Institute, number BR310161) and processed after 24hs, 48hs, 72hs, 96hs and 9 days by Transmission Electron Microscopy (TEM) technique. Our results showed cellular changes such as: mitochondria with electron-dense matrix and/or changes in the cristae, Golgi complex with dilated cisternae releasing vesicles with or without viral particles, dilated Endoplasmic Reticulum, electron- dense parallel tubular structures in the cytoplasm...(AU)

Effectiveness of a new endodontic irrigation system for removing smear layer and dissolving simulated organic matter.

OBJECTIVES: The study aimed to evaluate the potential for the dissolution of organic tissue in areas of simulated complexity and cleaning of root canal walls of the new iVac® endodontic irrigation system. MATERIAL AND METHODS: Thirty mandibular premolars were evaluated by scanning electron microscopy before and after biomechanical preparation. Then, they were distributed according to the final irrigation protocol into groups with conventional irrigation, ultrasonic agitation with metallic insert (UA), and iVac® system, and new photomicrographs were obtained. For tissue dissolution analysis, glass capillaries filled with catgut were attached to the cervical and apical thirds of twenty-one prototyped upper incisors. They were weighed before and after the previously mentioned irrigation protocols. The data were statistically compared with a significance level of 5%. RESULTS: The final irrigation provided greater cleaning of the root canal walls in the cervical, middle, and apical thirds of the root canals (P < 0.05), with no statistically significant difference between UA and iVac®, regardless of the analyzed thirds. Both ultrasonic irrigation protocols dissolved a significantly greater volume than the conventional irrigation protocol (P < 0.05), with no difference between the two protocols (P < 0.05). CONCLUSIONS: The iVac® system showed root canal wall cleaning and tissue dissolution similar to UA with a metallic insert, and both were superior to conventional irrigation. CLINICAL RELEVANCE: The new irrigation system iVac is more effective than conventional irrigation and has similar root canal wall cleaning and tissue dissolution to UA.

Social and health indicators in an academic community: enteroparasites, nutritional, allergic and clinical aspects / Indicadores sociais e de saúde em uma comunidade acadêmica: enteroparasitos, aspectos nutricionais, alérgicos e clínicos

Objective: this study aimed to associate enteroparasitic infection with nutritional, allergic, and clinical repercussions and the sociodemographic aspects in an academic community in Santo Antônio de Jesus, Bahia, Brazil. Methods: the cross-sectional research was carried out between February 2018 and March 2020, with 121 participants. Spontaneous sedimentation and Rugai techniques were used for the parasitological diagnosis of fecal samples. Allergies and clinical issues and the sociodemographic aspects were studied via a questionnaire, and the nutritional status was obtained from the assessment of body mass index and arm and abdominal circumferences. Results: there was 38.2% positivity for intestinal parasites; 65.9% for female participants; with the majority of eutrophic, between 18-27 years old; heteronormative, and single. Besides, the Endolimax nana (78.7%) and Giardia duodenalis (21.3%) were the predominant species. Statistical significance was found between obesity rates and the presence of intestinal parasites and those with allergic aspects. Nervousness, headache, and back and abdominal pains were the most common symptoms. Conclusions: this study contributes to obtaining data on epidemiological and public health surveys in the Bahian territory, providing a reference for other researchers. The statistical association of these with nutritional and allergic aspects can support future research on this population.

Objetivo: este estudo teve como objetivo associar a infecção enteroparasitária com aspectos nutricionais, alérgicos, clínicos e sociodemográficos em uma comunidade acadêmica do município de Santo Antônio de Jesus, Bahia, Brasil. Método: a pesquisa transversal foi realizada entre fevereiro de 2018 e março de 2020, com 121 participantes. As técnicas de sedimentação espontânea e Rugai foram utilizadas para o diagnóstico parasitológico de amostras fecais. Alergias, questões clínicas e aspectos sociodemográficos foram estudados por meio de um questionário, e o estado nutricional foi obtido por meio da avaliação do índice de massa corporal e das circunferências braquial e abdominal. Resultados: houve 38,2% de positividade para parasitos intestinais; 65,9% para participantes do sexo feminino; com a maioria eutrófica, entre 18-27 anos; heteronormativa e sigle. Além disso, Endolimax nana (78,7%) e Giardia duodenalis (21,3%) foram as espécies predominantes. Foi encontrada significância estatística entre as taxas de obesidade e presença de parasitos intestinais e com aspectos alérgicos. Nervosismo, dor de cabeça, dores nas costas e abdominais foram os sintomas mais comuns. Conclusões: este estudo contribui para a obtenção de dados sobre inquéritos epidemiológicos e de saúde pública no território baiano, servindo de referência para outras pesquisas. A associação estatística destes com aspectos nutricionais e alérgicos pode subsidiar futuras pesquisas nesta população.

Significados e ações frente à automutilação no contexto educacional brasileiro: revisão sistemática / The meanings and practices regarding self-mutilation in the brazilian educational context: a systematic review

Buscou-se descrever como a automutilação é compreendida pelas instituições de ensino e quais são as ações realizadas frente ao fenômeno, a partir de uma revisão sistemática de literatura. Em busca no Portal BVS, SciELO e no Catálogo de Teses da Capes, utilizando os descritores automutilação, autolesão, comportamento autolesivo e comportamento autodestrutivo, selecionou-se 20 documentos que foram submetidos à análise de conteúdo. Identificou-se que os estudos são recentes, predominantemente qualitativos, influenciados pela Análise do Comportamento e Psicanálise, e desenvolvidos junto a adolescentes de escolas públicas. A automutilação foi compreendida como decorrente de condições fisiológicas, forma de dar sentido ao sofrimento, resultado de contágio social, tentativa de comunicação, manifestação de ideação suicida e comportamento de indivíduos com desenvolvimento atípico. Há poucos relatos de intervenção frente ao problema, abarcando práticas clínicas, preventivas e de formação profissional. Destaca-se a necessidade da ampliação de práticas nas escolas compreendendo a automutilação numa perspectiva social e coletiva.

Se buscó describir cómo la automutilación es comprendida por las instituciones de enseñanza y cuáles las acciones realizadas frente al fenómeno, a partir de una revisión sistemática de literatura. En búsqueda en el Portal BVS, SciELO y en el Catálogo de Tesis de la Capes, utilizándose los descriptores automutilación, autolesión, comportamiento autolesivo y comportamiento autodestructivo, se seleccionó 20 documentos que se sometieron al análisis de contenido. Se identificó que los estudios son recientes, predominantemente cualitativos, influenciados por el Análisis del Comportamiento y Psicoanálisis, y desarrollados junto a adolescentes de escuelas públicas. La automutilación fue comprendida como resultante de condiciones fisiológicas, forma de dar sentido al sufrimiento, resultado de contagio social, tentativa de comunicación, manifestación de ideación suicida y comportamiento de individuos con desarrollo atípico. Hay pocos relatos de intervención frente al problema, abarcando prácticas clínicas, preventivas y de formación profesional. Se destaca la necesidad de la ampliación de prácticas en las escuelas comprendiendo la automutilación en una perspectiva social y colectiva.

In this article we sought to describe how self-mutilation is understood by educational institutions and what are the actions taken in the face of the phenomenon, based on a systematic literature review. Searching the BVS Portal, SciELO and the Capes Theses Catalog, using the index terms self-mutilation, self-injury, self-injurious behavior and self-destructive behavior, 20 documents were selected and submitted to content analysis. It was identified that the studies are recent, predominantly qualitative, influenced by Behavior Analysis and Psychoanalysis, and developed with adolescents from public schools. Self-mutilation was understood as resulting from physiological conditions, way of giving meaning to suffering, result of social contagion, communication attempt, manifestation of suicidal ideation and behavior of individuals with atypical development. There are few reports of intervention in the face of the problem, covering clinical, preventive and professional training practices. The need to implement interventions in schools, based on social and collective understandings of self-mutilation, is highlighted.

Repercussions of sickle cell disease and sickle cell ulcers for men inserted in the world of work / Repercusiones de la anemia de células falciformes y de las úlceras falciformes para los hombres insertados en el mundo del trabajo / Repercussões da anemia falciforme e das úlceras falcêmicas para homens inseridos no mundo do trabalho

ABSTRACT Objective: To analyze the repercussions of sickle cell disease and sickle cell ulcer for men in the world of work and discuss the challenges faced to remain in the work environment. Method: A qualitative study, developed at the dressing clinic and at a stomatherapy clinic. Twenty men with sickle cell disease and sickle cell ulcer participated, applying a semi-structured interview script. The software Interface de R pour les Analyses Multidimensionnelles de Textes et de Questionnaires was used for treatment and lexical data analysis. Results: The Descending Hierarchical Classification enabled the creation of classes: Man with sickle cell disease and sickle cell ulcer: experiences and repercussions; and Coping measures adopted by men with sickle cell disease and sickle cell ulcer to stay at work. Conclusion: Disease and injury repercussions involve biopsychosocial dimensions, highlighting the need for professional training to assist with competence and humanity. Strategies adopted to maintain work are breaks in the working day, use of analgesics to relieve pain, allocating time during work to apply dressings.

RESUMEN Objetivo: Analizar las repercusiones de la drepanocitosis y la drepanocitosis para hombres que actúan en el mundo del trabajo y discutir los desafíos enfrentados para permanecer en el ambiente de trabajo. Método: Estudio cualitativo, desarrollado en la clínica de vendajes y en una clínica de estomaterapia. Participaron 20 hombres con drepanocitosis y drepanocitosis, aplicando un guión de entrevista semiestructurada. Para el tratamiento y análisis léxico de los datos se utilizó el software Interface de R pour les Analyses Multidimensionnelles de Textes et de Questionnaires. Resultados: La Clasificación Jerárquica Descendente permitió la creación de clases: Hombre con anemia falciforme y úlcera falciforme: experiencias y repercusiones; y Medidas de afrontamiento adoptadas por el hombre con enfermedad de células falciformes y úlcera falciforme para permanecer en el trabajo. Conclusión: las repercusiones de la enfermedad y lesión involucran las dimensiones biopsicosociales, destacando la necesidad de formación profesional para asistir con competencia y humanidad. Las estrategias adoptadas para mantener el trabajo son pausas en la jornada laboral, uso de analgésicos para aliviar el dolor, asignación de tiempo durante el trabajo para aplicar curas.

RESUMO Objetivo: Analisar as repercussões da doença falciforme e da úlcera falcêmica para homens que atuam no mundo do trabalho e discutir os desafios enfrentados para se manterem no ambiente laboral. Método: Estudo qualitativo, desenvolvido no ambulatório de curativo e em uma clínica de estomaterapia. Participaram 20 homens com doença falciforme e úlcera falcêmica, aplicando-se roteiro de entrevista semiestruturado. Utilizou-se o software Interface de R pour les Analyses Multidimensionnelles de Textes et de Questionnaires, para tratamento e análise lexical dos dados. Resultados: A Classificação Hierárquica Descendente possibilitou a criação das classes: Homem com doença falciforme e úlcera falcêmica: vivências e repercussões; e Medidas de enfrentamento adotadas pelo homem com doença falciforme e úlcera falcêmica para manutenção no trabalho. Conclusão: As repercussões da doença e lesão envolvem as dimensões biopsicossociais, evidenciando a necessidade de capacitação dos profissionais para assistir com competência e humanidade. As estratégias adotadas para manutenção no trabalho são pausas na jornada laboral, uso de analgésicos para aplacar a dor, destinação de tempo durante o trabalho para realização dos curativos.

Implicit motor imagery performance and cortical activity throughout the menstrual cycle.

Studies show that female motor and visuospatial skills are modulated by the menstrual cycle. Implicit motor imagery, meaning the involuntary imagination of movements during a task, involves kinesthetic, visual, and spatial aspects of the corresponding action and can be investigated by using the hand laterality judgment task (HLJT). In this study we aimed to investigate whether implicit motor imagery performance and cortical activity are altered throughout the menstrual cycle, as demonstrated by motor skills in females. Thus, 31 healthy women underwent HLJT during the menstrual, follicular and luteal phases of their menstrual cycles. Right-handed participants had to recognize the laterality (right or left) of hands presented in different views (palm or back) and orientations on a computer screen. Test performance and EEG event-related potentials were analyzed. Participants performed better in the test in the follicular and luteal phases when compared to the menstrual phase, and the accuracy of the test was positively correlated with estradiol levels in the follicular phase. The difference between medial and lateral hand orientations for rotation-related negativity was significant in the medial and left parieto-occipital regions only in the follicular phase. These findings suggest positive modulating action of estradiol in performing implicit motor imagery.

Conhecimento científico sobre processo de enfermagem à pessoa idosa que vive com demência e seu cuidador / Scientific knowledge of the nursing process for older adults living with dementia and their caregivers / Conocimiento científico sobre el proceso de enfermería para las personas mayores que viven con demencia y su cuidador

Objetivo: mapear o conhecimento científico sobre o processo de enfermagem aplicado à pessoa idosa que vive com demência e seu cuidador. Método: revisão de escopo conforme o Instituto Joanna Briggs desenvolvido em três bases de dados. Resultados: dos 23 estudos incluídos, 11 fundamentaram o processo de enfermagem em bases teóricas, com ênfase na Teoria Geral do Déficit de Autocuidado. Para a coleta de dados, evidenciou-se instrumentos de avaliação de sintomas psicológicos e comportamentais, funcionalidade e avaliação da cognição/indicativos de demência. A maioria não especificou o uso de linguagem padronizada; e quando presente, a taxonomia North American Nursing Diagnosis Association foi a mais utilizada, com predominância de diagnósticos nos domínios percepção/cognição, segurança/proteção e atividade/repouso. Conclusão: os achados subsidiam a prática clínica do enfermeiro baseado em conhecimento científico atualizado e apontam para lacunas do uso de base teóricas e taxonomias para sustentar o processo de enfermagem.

Objective: to map the scientific knowledge of the nursing process applied to older adults living with dementia and their caregivers. Method: this scoping review was conducted in three databases following the Joanna Briggs Institute. Results: of the 23 studies included, 11 grounded the nursing process on theoretical bases, particularly the General Self-Care Deficit Theory. Data collection instruments were found to assess psychological and behavioral symptoms, functionality and cognition/indicators of dementia. Most did not specify use of standardized language; and when they did, the North American Nursing Diagnosis Association taxonomy was the most used, predominantly in the perception/cognition, safety/protection and activity/rest domains. Conclusion: the findings inform the clinical practice of nursing based on current scientific knowledge and point to gaps in the use of theoretical bases and taxonomies to support the nursing process.

Objetivo: mapear el conocimiento científico sobre el proceso de enfermería aplicado a los ancianos que viven con demencia y a su cuidador. Método: revisión de alcance según el Instituto Joanna Briggs desarrollada en tres bases de datos. Resultados: de los 23 estudios incluidos, 11 fundamentaron el proceso de enfermería en bases teóricas, con énfasis en la Teoría General del Déficit de Autocuidado. Para la recolección de datos, se utilizaron instrumentos de evaluación de síntomas psicológicos y conductuales, funcionalidad y evaluación de la cognición/indicios de demencia. La mayoría no especificó el uso de lenguaje estandarizado; y, cuando presente la taxonomía de la North American Nursing Diagnosis Association ha sido la más utilizada, con predominio de diagnósticos en los dominios de percepción/cognición, seguridad/protección y actividad/repudio. Conclusión: Los hallazgos subsidian la práctica clínica del enfermero con base en el conocimiento científico actualizado y apuntan hacia las lagunas del uso de base teórica y taxonomias para sustentar el proceso de enfermería.

Adaptive immunity to SARS-CoV-2 infection: A systematic review.

Background: There is evidence that the adaptive or acquired immune system is one of the crucial variables in differentiating the course of coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This work aimed to analyze the immunopathological aspects of adaptive immunity that are involved in the progression of this disease. Methods: This is a systematic review based on articles that included experimental evidence from in vitro assays, cohort studies, reviews, cross-sectional and case-control studies from PubMed, SciELO, MEDLINE, and Lilacs databases in English, Portuguese, or Spanish between January 2020 and July 2022. Results: Fifty-six articles were finalized for this review. CD4+ T cells were the most resolutive in the health-disease process compared with B cells and CD8+ T lymphocytes. The predominant subpopulations of T helper lymphocytes (Th) in critically ill patients are Th1, Th2, Th17 (without their main characteristics) and regulatory T cells (Treg), while in mild cases there is an influx of Th1, Th2, Th17 and follicular T helper cells (Tfh). These cells are responsible for the secretion of cytokines, including interleukin (IL) - 6, IL-4, IL-10, IL-7, IL-22, IL-21, IL-15, IL-1α, IL-23, IL-5, IL-13, IL-2, IL-17, tumor necrosis factor alpha (TNF-α), CXC motivating ligand (CXCL) 8, CXCL9 and tumor growth factor beta (TGF-ß), with the abovementioned first 8 inflammatory mediators related to clinical benefits, while the others to a poor prognosis. Some CD8+ T lymphocyte markers are associated with the severity of the disease, such as human leukocyte antigen (HLA-DR) and programmed cell death protein 1 (PD-1). Among the antibodies produced by SARS-CoV-2, Immunoglobulin (Ig) A stood out due to its potent release associated with a more severe clinical form. Conclusions: It is concluded that through this study it is possible to have a brief overview of the main immunological biomarkers and their function during SARS-CoV-2 infection in particular cell types. In critically ill individuals, adaptive immunity is varied, aberrantly compromised, and late. In particular, the T-cell response is also an essential and necessary component in immunological memory and therefore should be addressed in vaccine formulation strategies.

Diagnosis and successful treatment of Brazillian porcupine poxvirus infection in a free-ranging hairy dwarf porcupine (Coendu spinosus).

Brazilian porcupine poxvirus (BPoPV) is a new poxvirus recently described in porcupines (Coendou prehensilis) from Brazil. Herein, we described a free-ranging adult male Coendou (Sphiggurus) spinosus rescued after being found lethargic on the ground in a rural area. The animal presented crusty, edematous, and suppurative skin lesions on the face, tail, and perineum, and yellowish ocular secretion. The diagnosis was performed by histopathology, transmission electron microscopy (TEM), PCR, and sequencing. Microscopically, proliferative and necrotizing dermatitis, subacute, multifocal with ballooning degeneration, and eosinophilic intracytoplasmic viral inclusion bodies were observed. TEM confirmed large brick-shaped virions inside the keratinocyte cytoplasm, measuring about 200-280 × 120-180 nm. Partial fragment of intracellular mature virion membrane protein gene and putative metalloproteinase gene was successfully amplified and sequenced, and the strain herein denoted IAL/21 V-102 was classified as BPoPV, showing 99.4% of nucleotide identity to the reference strain UFU/USP001. Enrofloxacin 10% (10 mg/kg) was administered every 24 h through intramuscular injection for 10 days, dipyrone/metamizole (25 mg/kg) every 24 h orally (PO) for 3 days, 0.5 ml (mL) of thymomodulin every 24 h PO for 30 days, and each 48 h for another 15 days. The lesions were cleaned and debrided every 15 days. Seventy-five days after the beginning of the treatment, the cutaneous lesions regressed, the animal gained weight, and was clinically stable. After treatment, the skin biopsy showed only mild epidermal acanthosis, intra-cellular edema, and mild lymphoplasmacytic perivascular dermatitis. No viral particles were observed by TEM and no poxviral DNA was amplified by PCR. This study documents the first case of confirmed and treated BPoPV infection in a hairy dwarf porcupine. The implemented therapeutic plan eliminated the infection and improved the general state of the animal.

Violação dos direitos humanos dos pacientes com tentativa de suicídio no Brasil / Violation of the human rights of patients at risk of suicide attempt in Brazil

Resumo O suicídio é um grave problema de saúde pública mundial. Este estudo parte de um referencial teórico-normativo fundamentado nas normas de direitos humanos aplicáveis aos pacientes, no contexto dos cuidados em saúde. Objetivou-se investigar a violação dos direitos humanos dos pacientes em risco de suicídio. Foram realizadas 11 entrevistas semiestruturadas com profissionais da saúde, pacientes e familiares em quatro capitais brasileiras. Trata-se de uma pesquisa empírica qualitativa apoiada no método hermenêutico-dialético. Foi identificada a dificuldade dos profissionais em lidar com a temática, especialmente através da reprodução de condutas estigmatizantes, morais, religiosas, paternalistas e/ou hipervigilantes. Observou-se a violação dos direitos humanos dos pacientes, comprometendo o cuidado ofertado, a continuidade do tratamento, o protagonismo, a privacidade e a capacidade decisional do paciente, além do aumento da vulnerabilidade e do risco de novas tentativas. Entre os entrevistados, não houve consenso a respeito da conscientização das violações por parte dos profissionais. A proteção desses pacientes pressupõe a adoção de medidas de capacitação das equipes de saúde, processos de sensibilização e de informação para a sociedade, assim como a criação de políticas e legislações específicas, de modo a ampliar o entendimento sobre direitos humanos e prevenção do suicídio.

Abstract Suicide is a serious public health problem worldwide. This study's starting point is the theoretical-normative framework based on human rights standards applicable to the patients in the context of health care. The objective was to investigate the violation of the human rights of patients at risk of suicide. A total of 11 semi-structured interviews were conducted with health professionals, patients, and family members in four Brazilian state capitals. This study is a qualitative empirical research anchored by the hermeneutic-dialectic method. The professionals' difficulty in dealing with the subject was identified, especially by the reproduction of stigmatizing, moral, religious, paternalistic, and/or hyper vigilant behaviours. The violation of the human rights of patients was observed, compromising the care offered, the continuity of treatment, the protagonism, privacy, and the decision-making capacity of the patient, in addition to increasing the vulnerability and the risk of new attempts. Among the interviewees, there was no consensus regarding the awareness of violations on the part of professionals. The protection of these patients depends on the adoption of training measures for health teams, awareness and information processes for the society, and the creation of specific policies and legislation to broaden the understanding of human rights and suicide prevention.

MicroRNA-146a polymorphism is not associated with cardiovascular disease in the elderly / Polimorfismo MicroRNA 146a não está associado a doenças cardiovasculares em idosos

Abstract Introduction: Cardiovascular diseases (CVD) figure among the most significant causes of morbidity and mortality in the world and, among genetic factors, the literature has demonstrated the crucial role of miRNAs and the relationship of physical activity with this pathology. Objective: To investigate the relationship between the functional capacity of exercise, the level of physical activity, and the polymorphism in the miRNA-146a gene in elderly individuals with and without CVD. Methods: This study, developed in a city in the southern region of Brazil, is characterized as cross-sectional. The sample for this study comprised 342 participants, aged 60 or over. The following aspects were analyzed: anthropometric characteristics, genetic profiles, diagnosis of CVD, functional capacity, and the level of physical activity. Results: A statistically significant association was observed between CVD and body mass index (BMI) (א² = 14.278; p = 0.0003), and 40.6% of elderly individuals with CVD were obese, while 31.5% of the normally developed elderly participants presented normal BMI. However, the genotype frequencies (p = 0.546; א² = 1.211) and 6MWT (p = 0.311; א² = 1.025) did not show a statistically signifi-cant association with CVD. Conclusion: Our results suggest that the polymorphism in the miRNA-146A (rs2910164) and functional capacity are not associated with CVD in the elderly. However, the BMI did demonstrate an association with this disease.

Resumo Introdução: As doenças cardiovasculares (DCV) figuram entre as causas mais significativas de morbimortalidade no mundo e, dentre os fatores genéticos, a literatura tem demonstrado o papel crucial dos miRNAs e a relação da atividade física com essa patologia. Objetivo: Investigar a relação entre a capacidade funcional do exercício, o nível de atividade física e o polimorfismo no gene miRNA-146a em idosos com e sem DCV. Métodos: Este estudo, desenvolvido em um município da região sul do Brasil, caracteriza-se como transversal. A amostra deste estudo foi composta por 342 participantes, com idade igual ou superior a 60 anos. Foram analisados ​​os seguintes aspectos: características antropométricas, perfis genéticos, diagnóstico de DCV, capacidade funcional e nível de atividade física. Resultados: Observou-se associação estatisticamente significativa entre DCV e índice de massa corporal (IMC) (א² = 14,278; p = 0,0003), sendo que 40,6% dos idosos com DCV eram obesos, enquanto 31,5% dos idosos normalmente desenvolvidos apresentaram IMC normal. No entanto, as frequências genotípicas (p = 0,546; א² = 1,211) e TC6 (p = 0,311; א² = 1,025) não mostraram associação estatisticamente significante com DCV. Conclusão: Os resultados do presente estudo sugerem que o polimorfismo no miRNA-146A (rs2910164) e a capacidade funcional não estão associados à DCV em idosos; no entanto, o IMC demonstrou associação com essa doença.

Vozes e fazeres do semiárido: convites à descolonização do campo científico, rumo a outras práxis / Voices and actions in the semi-arid region: invitations to decolonize science, towards other praxis

RESUMO As fronteiras do capital neoextrativista avançam sobre territórios de populações tradicionais, provocando conflitos socioambientais, agravando a crise civilizatória, ameaçando a sustentação da vida no planeta. A pedagogia do território, a práxis acadêmica no Núcleo Tramas, traz pistas para que os sujeitos da universidade incidam na assimetria de forças presente nos territórios em conflito ambiental. Duas experiências iluminam as reflexões deste ensaio. Na luta pela construção do seu território camponês, as vivências das comunidades de Apodi/RN anunciam a agroecologia como forma de resistir ao Projeto da Morte. Por sua vez, o Núcleo de Reflexões, Estudos e Experiências em Agroecologia e Justiça Ambiental revelam o protagonismo das mulheres na construção da agroecologia e na defesa de seus territórios. Sob a perspectiva decolonial, discutem-se as bases teórico-epistemológicas, que incitam metodologias insurgentes e fomentam o diálogo de saberes, ressignificando os sujeitos cognoscentes. A mediação entre as vozes dos povos do semiárido e o campo científico da saúde coletiva provoca a reflexão: quais são os recados desses povos para a academia? Enquanto ainda se buscam possibilidades, os camponeses já têm, há muito, anunciado a agroecologia como alternativa para produzir, existir harmonicamente na natureza, promover saúde e resistir aos efeitos da colonialidade.

ABSTRACT Neoextractive capitalism advances over territories of traditional populations. The consequence is the increase in environmental conflicts and the deepening of the civilization crisis threatening life on the planet. Pedagogia do território (territory pedagogy), as an academic practice in the Núcleo Tramas, presents possibilities for the university to contribute to the reduction of the existing inequality in the context of environmental conflicts. Two reports help to reflect on this essay. In the struggle for the construction of peasant territory, the communities in the city of Apodi present agroecology as a way to resist the Projeto da Morte (Death Project). In turn, the Center for Reflections, Studies, and Experiences in Agroecology and Environmental Justice reveals the action of women in the construction of agroecology and in the defense of their territories. Based on decolonial theories, we discuss our theoretical and epistemological bases, which encourage insurgent methodologies and foster the dialogue of different knowledges. The mediation between the voices of semi-arid territories and the scientific field of collective health leads us to think: what are the messages from these peoples to the academy? While we are looking for possibilities, peasants are announcing agroecology as an alternative to produce, live harmoniously with nature, promote health, and resist the effects of coloniality.

Estágio em psicologia escolar e educacional e prevenção à criminalidade: ampliando perspectivas de atuação / Internship in school and educational psychology and crime prevention: expanding work perspectives

Este relato apresenta uma experiência de Estágio Básico em Psicologia Escolar e Educacional desenvolvida junto ao Programa Central de Acompanhamento de Penas e Medidas Alternativas (CEAPA), do Governo de Minas Gerais. Buscou-se relatar a complexidade dessa experiência a partir de três perspectivas: docentes, profissionais da instituição e uma estagiária. O Estágio, fundamentado na parceria entre instituição e universidade, foi importante desencadeador tanto de reflexões sobre o trabalho quanto de articulações teórico-práticas, além de aproximar os estagiários de possíveis campos de atuação - sobretudo aqueles que transcendem a estrutura da escola formal - e de desafios que se apresentam no cotidiano profissional.

This report presents a Basic Internship experience in School and Educational Psychology developed with the Central Program for the Monitoring of Penalties and Alternative Measures (CEAPA), of the Government of Minas Gerais. We sought to report the complexity of this experience from three perspectives: teachers, professionals at the institution and a female intern. The internship, based on the partnership between the institution and the university, was an important trigger for both reflections on work and theoretical and practical articulations, in addition to bringing trainees closer to possible fields of activity - especially those that transcend the structure of the formal school - and to challenges that present themselves in professional daily life.

Study of TNF-α, IFN-γ, TGF-ß, IL-6, and IL-10 Gene Polymorphism in Individuals from the Leprosy-Endemic Area in the Brazilian Amazon.

This study aimed at verifying the relationship between the polymorphisms of the cytokines tumor necrosis factor-alpha (TNF-α) -308 G → A (rs1800629); interferon gamma (IFN-γ) +874 T → A (rs2430561); transforming growth factor-beta (TGF-ß) códon 10 (rs1982073) and códon 25 (rs1800471); interleukin (IL)-6 - 174 G → C (rs180079) and IL-10 - 1082 A→T (rs1800896); -819 C → T (rs1800871); -592 A→C (rs1800872); and leprosy. Blood samples were analyzed from 106 individuals, of whom 24 were paucibacillary (PB), 28 were multibacillary (MB), and 54 were patient contacts. Analysis of cytokine polymorphisms was typified by the polymerase chain reaction technique. For TGF-ß +869 T → C and +915 G→C, a tendency to associate the presence of the C allele at codon 10 with leprosy was demonstrated, with the T allele being most frequently found in the CCOSI (P = 0.056). For the polymorphisms IL-10 - 1082 A→T, -819 C→T, and -592 A→C, we found an association of the GCC/GCC genotype with the susceptibility to the disease and the A allele at position 1082 with the leprosy protection. Greater predominance was found of ACC/ATA (31.3%) and GCC/ATA (37.5%) (P = 0.03) and the A allele at position -1082 (76.85%) (P = 0.043) in the CCOSI groups, whereas the GCC/GCC was found in the MB group (22.2%) (P = 0.05). For the other cytokines's single-nucleotide polymorphisms, there were no associations with susceptibility to leprosy. These results are limited by sample size, may not be conclusive, and will need further confirmation in a larger cohort.

A Clinical-Epidemiological and Geospatial Study of Tuberculosis in a Neglected Area in the Amazonian Region Highlights the Urgent Need for Control Measures.

Tuberculosis (TB) is an infectious communicable disease, which despite global efforts, still needs special attention in regions with difficult access. This study aims to describe the spatial and epidemiological trends of TB incidences from 2013 to 2018 in Marajó Island, the Amazonian region, Pará, Brazil. We have obtained secondary data from the Brazilian TB databases and performed geospatial and statistical analyses on the data for new TB cases, relapses, and re-admissions. From 2013 to 2018, 749 new cases were reported, in which the diagnostics (culture) was not performed for 652 (87.2%) patient samples, the drug resistance test (DST) was performed for nine (1.2%) samples, and one (0.13%) was multidrug-resistant TB (MDR-TB). The rapid molecular testing (RMT) was performed on 40 (5.3%) patient samples, with results indicating that eight (20%) were susceptible to rifampicin and two (5%) were rifampicin resistant. Overall, the cure rate was 449 (66.7%), while relapses and re-admissions were 41 and 44, respectively. On the geospatial distribution, the municipality of Soure stands out with a high number of incidences, relapses, and re-admissions. Spatially, the eight MDR cases were randomly distributed. Our data highlight the urgent need for TB control measures in this region, by introducing the Xpert-Ultra® MTB/RIF (Cepheid, Sunnyvale, CA, USA) and Ogawa-Kudoh.

Effect of oral nutritional supplements with or without nutritional counselling on mortality, treatment tolerance and quality of life in head-and-neck cancer patients receiving (chemo)radiotherapy: a systematic review and meta-analysis.

Oral nutritional supplements are widely recommended to head-and-neck cancer patients undergoing anti-cancer treatment; however, their effects on the outcomes of most importance to patients are unclear. This study aimed to systematically review the evidence of effect of oral nutritional supplements on mortality, treatment tolerance, quality of life, functional status, body weight and adverse effects. We searched PubMed, Embase, CENTRAL, LILACS, Web of Science, CINAHL, two trial registry platforms, three sources of grey literature and reference lists of included studies. We assessed the risk of bias using the revised Cochrane risk-of-bias tool (RoB 2), and certainty of evidence using the GRADE (Grading of Recommendations Assessment, Development and Evaluation) approach. We used random-effects models with Hartung-Knapp correction for the meta-analyses. We included fifteen trials, of which five were ongoing or unpublished, providing evidence in four comparisons. We found very low to low certainty evidence for the effect of oral nutritional supplements on mortality, treatment tolerance, quality of life, functional status and adverse effects. When compared with nutritional counselling alone, nutritional counselling plus oral nutritional supplements probably increased body weight slightly. We also found adverse events relating to supplements use such as nausea, vomiting and feeling of fullness. Possible increases in mortality, treatment tolerance and quality of life besides a possible decrease in functional status are worthy of further investigation. Future research could attempt to address the clinical importance of a probable increase in body weight. Possible adverse effects of the use of oral nutritional supplements should not be overlooked.

The performance of nursing students in a tele-health service during the COVID-19 pandemic / Desempeño de estudiantes de enfermería en un servicio de tele-salud durante la pandemia COVID-19 / Atuação de estudantes de enfermagem em um serviço de telessaúde durante a pandemia COVID-19

ABSTRACT Objective: To identify the experiences of nursing students working in a telehealth service to fight the COVID-19. Method: Qualitative, exploratory, and descriptive study, addressing 31 undergraduate nursing students at a Federal University located in the extreme south of Brazil. Data were collected in October 2020 using an online questionnaire. Textual discourse analysis was used to interpret data. Results: The students identified facilities, weaknesses, and potentialities of a telehealth service, verifying that it is a strategy that favors the development of skills and competencies during the training of students and to fight the COVID-19 pandemic. Conclusion: The results reveal the students' experiences in a telehealth service designed to fight the COVID-19, showing the importance of including this service in the routine of healthcare services.

RESUMEN Objetivo: Conocer las experiencias de estudiantes de enfermería que trabajan en un servicio de telesalud para enfrentar el COVID-19. Método: Estudio cualitativo, exploratorio y descriptivo, realizado con 31 estudiantes de pregrado en enfermería de una Universidad Federal ubicada en el extremo sur de Brasil. Los datos fueron recolectados en octubre de 2020, a través de un cuestionario en línea, en el que se realizó un análisis textual discursivo. Resultados: Se identificaron las facilidades, debilidades y potencialidades en el desempeño de los estudiantes de enfermería en un servicio de telesalud, demostrando que constituye una estrategia favorable tanto para el desarrollo de habilidades y competencias en la educación de los estudiantes como para el afrontamiento de la pandemia COVID-19. Conclusión: Se encontró que el estudio logró identificar las experiencias de los estudiantes en relación al trabajo en un servicio de telesalud en el afrontamiento de COVID-19, demostrando la importancia de incluir este servicio en la atención diaria de la salud.

RESUMO Objetivo: Conhecer as experiências de estudantes de enfermagem na atuação em um serviço de telessaúde para enfrentamento da COVID-19. Método: Estudo qualitativo, exploratório e descritivo, realizado com 31 estudantes de graduação em enfermagem de uma Universidade Federal localizada no extremo Sul do Brasil. Os dados foram coletados em outubro de 2020, por meio de um questionário online, no qual procedeu-se a análise textual discursiva. Resultados: Foram identificadas as facilidades, fragilidades e potencialidades na atuação de estudantes de enfermagem em um serviço de telessaúde, constatando-se que o mesmo constitui-se de uma estratégia favorável tanto para o desenvolvimento de habilidades e competências na formação dos estudantes quanto para o enfrentamento da pandemia COVID-19. Conclusão: Constatou-se que o estudo conseguiu identificar as experiências dos estudantes em relação à atuação em um serviço de telessaúde no enfrentamento da COVID-19, demonstrando a importância de incluir esse serviço no cotidiano do cuidado em saúde.

Autoeficácia em amamentar entre mães de bebês prematuros / Self-effectiveness in breast-feeding between mothers of premature babies / Autoeficiencia en la alimentación materna entre madres de bebés prematuros

Objetivo: avaliar a autoeficácia em amamentar entre mães de bebês prematuros. Métodos: estudo descritivo, transversal, com abordagem quantitativa, realizado no Hospital Maternidade do município de Quixadá no período de setembro a outubro de 2017. A amostra foi composta por 20 puérperas abordadas no alojamento conjunto por meio de uma entrevista para aplicação do formulário contendo dados sociodemográficos e obstétricos e a escala Breastfeeding Self-Efficacy Scale ­ Short-Form. Os resultados apresentados por meio de tabelas. Resultados: todas as mães apresentaram elevada autoeficácia em amamentar. Os itens da escala de menores pontuações entre as mulheres foram: "Eu sempre amamento meu bebê em um peito e depois mudo para o outro" e "Eu sempre posso amamentar mesmo se o meu bebê estiver chorando". Conclusão: percebe-se a importância do enfermeiro na atuação durante todo o ciclo gravídico puerperal no intuito de manter a confiança materna, bem como focar em aspectos que possam interferir na mesma

Objective: to evaluate breastfeeding self-efficacy among mothers of premature babies. Methods: a descriptive cross-sectional study with a quantitative approach, carried out at the Maternity Hospital of the city of Quixadá, from September to October 2017. The sample consisted of 20 mothers approached in the rooming-in through an interview to apply the form containing data. sociodemographic and obstetric and the Breastfeeding Self-Efficacy Scale - Short-Form. Results are presented by tables. Results: all mothers showed high self-efficacy in breastfeeding. The lowest scoring items among women were: "I always breastfeed my baby on one breast and then move to the other" and "I can always breastfeed even if my baby is crying". Conclusion: it is perceived the importance of nurses in their performance throughout the puerperal pregnancy cycle in order to maintain maternal confidence, as well as focus on aspects that may interfere with it

Objetivo: evaluar la autoeficacia de la lactancia materna en madres de bebés prematuros. Métodos: un estudio descriptivo de corte transversal con enfoque cuantitativo, realizado en el Hospital de maternidad de la ciudad de Quixadá, de septiembre a octubre de 2017. La muestra estuvo compuesta por 20 madres abordadas en la habitación a través de una entrevista para aplicar el formulario que contiene los datos. Sociodemográfico y obstétrico y la escala de autoeficacia de la lactancia materna - Forma corta. Los resultados se presentan por tablas. Resultados: todas las madres mostraron una alta autoeficacia en la lactancia materna. Los puntos de puntuación más bajos entre las mujeres fueron: "Siempre amamanto a mi bebé en un pecho y luego me muevo al otro" y "Siempre puedo amamantar aunque mi bebé esté llorando". Conclusión: se percibe la importancia de las enfermeras en su desempeño a lo largo del ciclo del embarazo puerperal para mantener la confianza materna, así como para centrarse en los aspectos que pueden interferir con ella