RESUMEN
A urease-producing Gram-stain-positive actinobacterium, designated strain T5T, was isolated from a soil sample collected at a highway hillslope in Selangor, Malaysia. The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar. Strain T5T grew at 15-37 °C, pH 6-11, and tolerated up to 9â% (w/v) NaCl, with optimal growth occurring at 28 °C, pH 6-9 and without NaCl. The whole-cell sugar hydrolysate of strain T5T contained galactose, glucose and ribose. The ll-diaminopimelic acid isomer was detected in the cell wall. Diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were found to be the predominant polar lipids. The main fatty acids were anteiso-C17â:â0, iso-C16â:â0, anteiso-C15â:â0 and iso-C14â:â0. Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0â%). The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4â% average nucleotide identity and 33.7â% digital DNA-DNA hybridization. Phylogeny analyses showed that strain T5T was closely related to Streptomyces fradiae, Streptomyces lavendofoliae, Streptomyces lichenis, Streptomyces roseolilacinus and Streptomyces somaliensis. Based on these polyphasic data, strain T5T represents a novel species, for which the name Streptomyces solincola sp. nov. is proposed. The type strain is T5T (=TBRC 5137T= DSM 42166T).
Asunto(s)
Fosfatidiletanolaminas , Streptomyces , ARN Ribosómico 16S/genética , Filogenia , Ácido Diaminopimélico/análisis , Suelo , Galactosa , Ribosa , Cardiolipinas , Cloruro de Sodio , Agar , Ureasa/genética , Malasia , Composición de Base , Ácidos Grasos/química , ADN Bacteriano/genética , Técnicas de Tipificación Bacteriana , Fosfolípidos/análisis , Análisis de Secuencia de ADN , Glucosa , Fosfatidilcolinas , Fosfatidilinositoles/análisis , NucleótidosRESUMEN
A bacterial isolate, designated strain S37T, was isolated from the rhizosphere of oil palm (Elaeis guineensis). Strain S37T was found to be Gram-stain-negative, aerobic, motile and rod shaped. Based on 16S rRNA gene sequence analysis, strain S37T was most closely related to Devosia albogilva IPL15T (97.3â%), Devosia chinhatensis IPL18T (96.8â%) and Devosia subaequoris HST3-14T (96.5â%). The G+C content of the genomic DNA was 63.0 mol%, and dominant cellular fatty acids were summed feature 8 (C18â:â1ω7c and/or C18â:â1ω6c), 11-methyl C18â:â1ω7c and C16â:â0. The predominant isoprenoid quinone was ubiquinone-10 (Q-10), and the major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, glycolipid and phospholipids. Based on the polyphasic taxonomic data, it is clear that strain S37T represents a novel species of the genus Devosia within the family Hyphomicrobiaceae, for which we propose the name Devosia elaeis sp. nov., with strain S37T (=TBRC 5145T=LMG 29420T) as the type strain.