[Antimutagenic and antioxidant features of confectionery products containing the powder from the leaves of Hippophae rhamnoides L.]

The aim of the work was to determine the possibility of using the powder from the leaves of Hippophae rhamnoides L. for enriching flour confectionery and to evaluate the antimutagenic and antioxidant activity of the product. The experiment was carried out on 24 white Wistar rats with initial body weight (b.w.) 180-200 g. The animals of the experimental group (n=8) received confection containing sea buckthorn powder at a rate of 20 mg per 100 g b.w. for 14 days on the background of a standard vivarium diet. The animals of the control and intact groups received confection containing no bioactive supplement at the same dose. Antimutagenic and antioxidant effects were estimated in a day after a single injection of cyclophosphamide at a dose of 20 mg/kg b.w. The number of chromosomal aberrations in bone marrow cells of white rats was counted and the activity of catalase, superoxide dismutase (SOD), the level of reduced glutathione and the concentration of TBA-active products in blood were evaluated. The intake of the confectionery containing the powdered H. rhamnoides leaves resulted in the 45% decrease of the number of damaged cells, 50% decrease of the proportion of cells with multiple chromosome breaks and 52% decrease of the number of achromatic gaps as compared to animals of the control group (n=8). The cake intake increased the activity of catalase (by 52%) and SOD (by 33%) and glutathione content (by 26%) in blood.

[Effect of Hypecoum erectum extract on morphofunctional state of the liver in rats with tetracycline-associated hepatitis].

The effect of Hypecoum erectum L. extract on the morphofunctional condition of the liver in rats with experimental tetracycline-associated hepatitis was studied. The experiment included 40 albino rats Wistar. The animals treated with tetracycline hydrochloride (1.0 g/kg body weight) were exposed to the extract in a dose of 50 mg/kg for 5 days. On the 7th day of the experiment the following indices were determined: malonic dialdehyde concentration, catalase activity, the levels of ATP, pyruvate and lactate in the liver homogenate, as well as the blood levels of reduced glutathione. The liver pathomorphological investigation was applied. The H. erectum extract was shown to inhibit lipid peroxidation, to increase the activity of the host endogenous antioxidant system, to normalize the hepatocyte energy provision and to limit the liver degeneration.

[Radio protective drug production from fresh leaves of Aloe arborescens Mill].

Nowadays, phytogenous drugs are wildly used as radio protective substances. The aim of the research was to study radio protective characteristics of aloe juice fraction and to develop new technology for radio protective drug production. Technological scheme for getting the drug in two stages. The first stage - extraction of juice from fresh leaves; the second stage - extracting bagasse have been developed and optimal environment for bagasse extraction are defined: Infusion of bagasse with 96 % ethyl spirit (1:1) during 30 minutes, continuation of extracting with water on correlation to raw materials 10:1 at temperature of 70 degrees C during 30 minutes. For the basis of the first series of balanced loading there are taken the optimal parameters of extracting process, on the basis of which in its turn was developed technological scheme of getting dry extract of aloe. Dry extract is a fine-dispersed reddish-yellow (brownish-yellow) powder, which can be easily dissolved in warm (40-60 degrees C) water. Pharmacological researches were conducted in the Institute of General and Experimental Biology, Siberian Branch, Russian. Academy of Sciences. The remarkable radio protective effect of the drug was revealed.

[Hepatoprotective effect of hepatophyt in experimental drug hepatitis].

The hepatoprotective action of Hepatophyt was studied on Wistar rats with experimental hepatitis caused by exposure of the animals to tetracycline. Hepatophyt was administered per os in a dose of 0.1 g/kg. Its significant pharmacotherapeutic action was due to the content of biologically active substances, mainly of polyphenol nature. As natural antioxidants, they provided stabilization of the biomembranes and increased the energetic potential of the hepatic cells and the contractile capacity of the pericanal microfilaments, thus stimulating the synthesis and transport of the cholates and excretion of bilirubin and cholesterol with bile.

[Phytocorrection of structural changes in the gastrointestinal tract caused by 6-mercaptopurine].

It was shown that the course use of Ventrofit, a complex plant remedy, normalized the structural and functional integrity of the stomach and thin intestine mucous membranes damaged by the use of 6-mercaptopurine.

[The characteristics of the action of polyphytochol on the course of experimental hepatitis and cholecystitis]. / Osobennosti deistviia polifitokhola na techenie éksperimental'nogo gepatita i kholetsistita.

The effect of polyphytochol representing a combination of vegetable agents was studied on models of acute toxic hepatitis caused by CCl4 and cholecystitis. The efficiency of the drug in pathology of the hepatobiliary system was shown: it decreases cholestasis acting as a choleretic and bile-secreting drug, acts on the main pathogenetic links in hepatitis, exerts a marked membrane-stabilizing effect, influences favourably the processes of synthesis in the liver.

[Effect of kaleflon on the course of experimental hepatitis]. / Vliianie kaleflona na techenie éksperimental'nogo gepatita.

A pronounced cholagogic effect of kaleflon was found in the experiment on albino rats. The course administration of kaleflon in a dose of 0.05 g/kg exerts a marked pharmacotherapeutic action on the course of experimental hepatitis.

[Experimental antioxidant pharmacotherapy of liver injuries]. / Eksperimental'naia farmakoterapiia antioksidantami povrezhdenii pecheni.

It has been shown in rats with experimental hepatitis that sodium selenite and vegetable polyphenol complexes of Malus Pallasiana Juz., Schabiosa comosa F ex. R. et Sch., Odontites serotina Dum., Gentiana barbata Froel, have antioxidant action. Experiments on rabbits and rats have demonstrated the pathogenetic importance of activation of free-radical lipid oxidation during ischemic and toxic injuries to the liver and biliferous ducts.