RESUMEN
A quasi-defined medium that supports the growth of Streptococcus agalactiae as pigmented colonies has been developed. The medium contains starch, a peptic digest of albumin, amino acids, nucleosides, vitamins, and salts. The presence of free cysteine, which could be replaced with other sulphur-containing compounds and to a lesser degree by reducing agents, was required for pigment formation.
Asunto(s)
Pigmentos Biológicos/metabolismo , Infecciones Estreptocócicas/microbiología , Streptococcus agalactiae/crecimiento & desarrollo , Streptococcus agalactiae/aislamiento & purificación , Técnicas Bacteriológicas , Medios de Cultivo/química , Humanos , Streptococcus agalactiae/metabolismoRESUMEN
OBJECTIVES: We have contributed to the Design Validation Protocol of the new Abbott AxSYM Testosterone assay with a study on reference values and methods comparison. DESIGN AND METHODS: For reference values a population of 45 women and 30 men was tested. In methods comparison, 132 samples for the AxSYM vs. ACS-180, and 30 for the AxSYM vs. Elecsys were used. Pearson and intraclass concordance coefficients and Passing-Bablock test were performed for overall group, men and women. RESULTS: Reference values were 0.9-3.1 (females) and 1.0-30.2 nmol/L (males). Globally, a good agreement between methods in both the AxSYM vs. ACS-180 (slope: 0.88, y-intercept: 0.67, r = 0.961) and the AxSYM vs. Elecsys (slope: 1.08, y-intercept: 0.31, r = 0.935) studies was found. Slightly worse results were observed for women. CONCLUSIONS: The reference range of testosterone by the AxSYM system matches with those published for other methods. An acceptable agreement between the AxSYM Testosterone assay and both a classical (ACS-180) and a more recent (Elecsys) methodology was observed.
Asunto(s)
Técnicas para Inmunoenzimas , Testosterona/sangre , Adolescente , Adulto , Análisis de Varianza , Donantes de Sangre , Niño , Estudios de Evaluación como Asunto , Femenino , Humanos , Técnicas para Inmunoenzimas/instrumentación , Técnicas para Inmunoenzimas/estadística & datos numéricos , Masculino , Persona de Mediana Edad , Valores de Referencia , Estadística como Asunto , Estadísticas no ParamétricasRESUMEN
A case of calcified gastric adenocarcinoma is presented. It is of clinical interest because of its rareness. Histologically a mucus-secreting adenocarcinoma with lakes of intercellular mucin was found. The need to take this lesion into account in the differential diagnosis of the calcifications in the upper left quadrant, is emphasized.