Laurocapram, a transdermal enhancer, boosts cephalosporin's antibacterial activity against Methicillin-resistant Staphylococcus aureus.

Methicillin-resistant Staphylococcus aureus (MRSA), a notorious bacterium with high drug resistance and easy recurrence after surgery, has posed significant clinical treatment challenges. In the current scarcity of new antibiotics, the identification of adjuvants to existing antibiotics is a promising approach to combat infections caused by multidrug-resistant Gram-positive bacteria. The in vitro synergy test, which included a MIC assay, time-kill curve, antimicrobial susceptibility testing, and live/dead bacteria staining assay, revealed that laurocapram, a widely used chemical transdermal enhancer, could potentiate the antibacterial activity of cephalosporins against MRSA. In vitro, laurocapram combined with cefixime showed an excellent synergistic activity against MRSA (FICI = 0.28 ± 0.00). In addition, the combination of laurocapram and cefixime may inhibited the formation of MRSA biofilm and caused cell membrane damage. Following that, we discovered that combining laurocapram with cefixime could alleviate the symptoms of mice in the MRSA skin infection model and the MRSA pneumonia model. In conclusion, laurocapram is a promising and low-cost antibacterial adjuvant, providing a new strategy for further exploring the use of lower doses of cephalosporins to combat MRSA infection.

The enhancement of benzene total oxidation over RuxCeO2 catalysts at low temperature: The significance of Ru incorporation.

Catalytic oxidation is considered to be the most efficient technology for eliminating benzene from waste gas. The challenge is the reduction of the catalytic reaction temperature for the deep oxidation of benzene. Here, highly efficient RuxCeO2 catalysts were utilized to turn the number of surface oxygen vacancies and Ce-O-Ru bonds via a one-step hydrothermal method, resulting in a preferable low-temperature reducibility for the total oxidation of benzene. The T50 of the Ru0.2CeO2 catalyst for benzene oxidation was 135 °C, which was better than that of pristine CeO2 (239 °C) and 0.2Ru/CeO2 (190 °C). The superior performance of Ru0.2CeO2 was attributed to its large surface area (approximately 114.23 m2·g-1), abundant surface oxygen vacancies, and Ce-O-Ru bonds. The incorporation of Ru into the CeO2 lattice could effectively facilitate the destruction of the CeO bond and the facile release of lattice oxygen, inducing the generation of surface oxygen vacancies. Meanwhile, the bridging action of Ce-O-Ru bonds accelerated electron transfer and lattice oxygen transportation, which had a synergistic effect with surface oxygen vacancies to reduce the reaction temperature. The Ru0.2CeO2 catalyst also exhibited high catalytic stability, water tolerance, and impact resistance in terms of benzene abatement. Using in situ infrared spectroscopy, it was demonstrated that the Ru0.2CeO2 catalyst can effectively enhance the accumulation of maleate species, which are key intermediates for benzene ring opening, thereby enhancing the deep oxidation of benzene.