Influence of oblique proximal ulnar osteotomy on humeral intracondylar fissures in 35 spaniel breed dogs.

OBJECTIVE: To investigate the effects of oblique proximal ulnar osteotomy (PUO) on the healing of humeral intracondylar fissure (HIF) in spaniel breed dogs. STUDY DESIGN: Clinical retrospective study. SAMPLE POPULATION: A total of 51 elbows from 35 spaniel dogs. METHODS: An oblique PUO was performed in dogs diagnosed with HIF. The degree of healing of HIF was subjectively and objectively assessed on preoperative and long-term follow-up CT imaging. Objective assessment was performed by measuring the bone density in Hounsfield units (HU) of a rectangular region of interest (ROI) encompassing the entire hypoattenuated humeral fissure. Major and minor complications were recorded. RESULTS: A total of 24 partial and 27 complete HIFs were diagnosed. The follow-up CT scan was performed at a median 18.5 months (range 10-49 months). Subjective assessment confirmed partial or complete healing of the HIF in 41 elbows (80.3%). Objective assessment confirmed a difference in mean HU of the HIF's ROI between preoperative (HU 640) and last follow-up CT images (HU 835) (p = .001). Young dogs (<14 months) had the highest increase in HU of the HIF's ROI. Major complications occurred in five dogs (6 limbs) of which four were related to the lack of healing of the fissure (7.8%). CONCLUSION: Oblique PUO resulted in partial or complete healing of HIF and pain resolution in the majority of dogs. CLINICAL SIGNIFICANCE: This study introduces an innovative approach to achieve healing of the HIF in the dog, which may help reduce the high complication rate traditionally associated with the use of transcondylar screws.

Pesticides luminescent sensing by a Tb3+-doped Zn metal-organic framework with selectivity towards parathion.

Organophosphorus pesticides (OPPs) such as parathion have extensive uses in agriculture and household applications. Chronic exposure to these pesticides can cause severe health and environmental issues. Therefore, a current ecological concern is associated with accumulating these noxious OPPs in food and water sources. In this work, a new Tb3+-doped Zn-LMOF (Zn-LMOF= (3D) {[Zn3(1,4 benzenedicarboxylate)3(EtOH)2]·(EtOH)0.6}∞) was synthesized by a solvent-free reaction between the Zn-LMOF and the salt TbCl3·6H2O using a high-speed ball milling. The Tb@Zn-LMOF was thoroughly characterized by multiple spectroscopic tools, including Scanning Electron Microscopy with Energy-Dispersive X-ray Spectroscopy, and studied in-depth as a luminescent sensor for a series of pesticides (parathion, malathion, methalaxil, carbofuran, iprodione, captan and glyphosate) in aqueous methanol. The Tb@Zn-LMOF is a long-lived green-emitting compound with luminescence originated by an efficient antenna effect from the excited energy levels of Zn-LMOF toward the 5D state of Tb3+ ions, as it is displayed by its strong emission bands at 488, 545, 585, and 620 nm and a lifetime of 1.01 ms upon excitation at 290 nm. Additions of pesticides to a neutral methanolic dispersion of Tb@Zn-LMOF modified its green emission intensity with a pronounced selectivity toward parathion within the micromolar concentration range. The detection limit for parathion was calculated to be 3.04 ± 0.2 µM for Tb@Zn-LMOF. Based on 31P NMR and mass spectrometry studies, it is attributed to the release of lanthanide ions from Tb@Zn-LMOF with the simultaneous formation of a Tb3+-parathion complex.

Dynamic allosteric networks drive adenosine A1 receptor activation and G-protein coupling.

G-protein coupled receptors (GPCRs) present specific activation pathways and signaling among receptor subtypes. Hence, an extensive knowledge of the structural dynamics of the receptor is critical for the development of therapeutics. Here, we target the adenosine A1 receptor (A1R), for which a negligible number of drugs have been approved. We combine molecular dynamics simulations, enhanced sampling techniques, network theory and pocket detection to decipher the activation pathway of A1R, decode the allosteric networks and identify transient pockets. The A1R activation pathway reveal hidden intermediate and pre-active states together with the inactive and fully-active states observed experimentally. The protein energy networks computed throughout these conformational states successfully unravel the extra and intracellular allosteric centers and the communication pathways that couples them. We observe that the allosteric networks are dynamic, being increased along activation and fine-tuned in presence of the trimeric G-proteins. Overlap of transient pockets and energy networks uncover how the allosteric coupling between pockets and distinct functional regions of the receptor is altered along activation. By an in-depth analysis of the bridge between activation pathway, energy networks and transient pockets, we provide a further understanding of A1R. This information can be useful to ease the design of allosteric modulators for A1R.

Wearable Sports Technology Development in Costa Rica: Inertial Measurement Unit Integration for Real-Time External Load Monitoring / Desarrollo de tecnología deportiva portátil en Costa Rica: integración de una unidad de medición inercial para monitoreo de carga externa en tiempo real / Desenvolvimento de tecnologia desportiva portátil na Costa Rica: integração de uma unidade de medição por inércia para monitorização da carga externa em tempo real

Abstract: The increasing trend and projection for the use of wearable sensors technology in the general population have led to the development of different inertial measurement units (IMU) to monitor activity and motion variables during exercise or sports. This study aims to systematize the practical experience of developing an inertial measurement device to quantify external load in real time for runners. This article shows the installed capacity of Costa Rican higher education institutions to integrate existing sensors available in the international market in a single device to program it and use it for different sport science purposes. Two scientific laboratories joined resources and integrated an interdisciplinary team composed of exercise physiologists, sports scientists, physiotherapists, and electromechanical engineers who globally faced the challenge of creating SafeRun IMU. In this case, an IMU was constructed with great success, capable of quantifying external load variables controlled from a mobile device in real-time. The results showed an acceptable agreement and reliability of the data extracted from the IMU. The information collected from this IMU could be used to control and quantify external load to develop or redirect exercise programming and prescription and prevent overuse injuries or physical overload conditions. This device differs from other existing ones due to its small size and portability, which benefits its commercialization and massification.

Resumen: La creciente tendencia y proyección para el uso de tecnología de sensores portátiles en la población en general ha llevado al desenvolvimiento de diferentes dispositivos de medición inercial, para monitorear las variables de actividad y movimiento durante el ejercicio o los deportes. El objetivo del presente estudio es sistematizar la experiencia práctica de desarrollar un dispositivo de medición inercial, con el fin de cuantificar la carga externa en tiempo real para los corredores. Este artículo demostró la capacidad instalada de las instituciones de educación superior de Costa Rica para integrar los sensores existentes disponibles en el mercado internacional en un solo dispositivo, con el propósito de programarlo y usarlo en diferentes fines de ciencias del deporte. Dos laboratorios científicos unieron esfuerzos e integraron un equipo interdisciplinario compuesto por fisiólogos del ejercicio, científicos del deporte, fisioterapeutas e ingenieros electromecánicos, quienes, de manera global, afrontaron el reto de la creación de la IMU. En este caso, se desarrolló una IMU con gran éxito, capaz de cuantificar las variables de carga externa controladas desde un dispositivo móvil en tiempo real. Los resultados mostraron un nivel de concordancia y fiabilidad aceptable de los datos extraídos de la IMU. La información obtenida de esta IMU podría usarse para controlar y cuantificar la carga externa, con miras a desplegar o redirigir la programación y prescripción de ejercicios, así como con el afán de prevenir lesiones por sobreuso o condiciones de sobrecarga física.

Resumo: A crescente tendência e projeção para o uso da tecnologia de sensores portáteis na população em geral levou ao desenvolvimento de diferentes dispositivos de medição inercial para monitorar as variáveis de atividade e de movimento durante o exercício ou esportes. O objetivo do presente estudo é sistematizar a experiência prática de desenvolvimento de um dispositivo de medição inercial, a fim de quantificar a carga externa em tempo real para os corredores. Este artigo demonstrou a capacidade instalada das instituições de ensino superior da Costa Rica para integrar os sensores existentes disponíveis no mercado internacional em um único dispositivo, com a finalidade de programá-lo e usá-lo para diferentes fins de ciência do esporte. Dois laboratórios científicos uniram forças e integraram uma equipe interdisciplinar composta por fisiologistas do exercício, cientistas do esporte, fisioterapeutas e engenheiros eletromecânicos, que, de forma global, enfrentaram o desafio de criar a IMU. Neste caso, foi desenvolvida uma IMU de grande sucesso, capaz de quantificar variáveis de carga externas controladas a partir de um dispositivo móvel em tempo real. Os resultados mostraram um nível aceitável de concordância e confiabilidade dos dados extraídos da IMU. As informações obtidas desta IMU poderiam ser utilizadas para monitorar e quantificar a carga externa, com o objetivo de implantar ou redirecionar a programação e prescrição de exercícios, bem como com o objetivo de evitar lesões por uso excessivo ou condições de sobrecarga física.

Chemical Characterization, Antioxidant Capacity and Anti-Oxidative Stress Potential of South American Fabaceae Desmodium tortuosum.

It has been proposed that oxidative stress is a pathogenic mechanism to induce cytotoxicity and to cause cardiovascular and neuronal diseases. At present, natural compounds such as plant extracts have been used to reduce the cytotoxic effects produced by agents that induce oxidative stress. Our study aimed to evaluate the antioxidant and cytoprotective capacity of Desmodium tortuosum (D. tortuosum) extract in the co- and pre-treatment in EA.hy926 and SH-SY5Y cell lines subjected to oxidative stress induced by tert-butylhydroperoxide (t-BOOH). Cell viability, reactive oxygen species (ROS), nitric oxide (NO), caspase 3/7 activity, reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione reductase (GR), and molecular expression of oxidative stress biomarkers (SOD2, NRF2 and NFκB1) and cell death (APAF1, BAX, Caspase3) were all evaluated. It was observed that the D. tortuosum extract, in a dose-dependent manner, was able to reduce the oxidative and cytotoxicity effects induced by t-BOOH, even normalized to a dose of 200 µg/mL, which would be due to the high content of phenolic compounds mainly phenolic acids, flavonoids, carotenoids and other antioxidant compounds. Finally, these results are indicators that the extract of D. tortuosum could be a natural alternative against the cytotoxic exposure to stressful and cytotoxic chemical agents.

Functional outcomes of dogs undergoing shoulder arthrodesis with 2 locking compression plates.

OBJECTIVE: To report the outcomes of dogs undergoing shoulder arthrodesis with the application of 2 locking compression plates (LCPs). STUDY DESIGN: Retrospective case series. ANIMALS: Twelve client owned dogs undergoing shoulder arthrodesis using 2 LCPs as the principal method of fixation. METHODS: Medical records were searched for dogs who had undergone shoulder arthrodesis at a hospital between 2014-2021. Inclusion criteria required at least 6 months postoperative follow up and the use of 2 LCPs as the principal method of arthrodesis. Outcomes recorded include clinical assessment, Liverpool Osteoarthritis in Dogs (LOAD) scoring, force plate analysis, and a client visual-analogue scale with at least 6 months follow up. RESULTS: All shoulders were immobilized with 2 LCPs and no postoperative external coaptation. The duration of follow up was 855.9 days (174-2135). Minor complications were recorded in 2/12 dogs, neither requiring surgical revision. Preoperative to postoperative mean lameness scores reduced from 2.9 to 0.8 (0-5), with 11/12 dogs making a full functional recovery, with a mean long-term LOAD score of 12.9 (4-23). Gait analysis was available in 5/12 dogs, placing a mean of 31.9% of their bodyweight on the operated limb, with a mean symmetry index of 8.7%. Owners scores (n = 11/12) were consistent with a high level of satisfaction (9.6/10). CONCLUSION: Shoulder arthrodesis with 2 LCPs was associated with low morbidity and good outcomes in our clinical setting. CLINICAL SIGNIFICANCE: Canine shoulder arthrodesis performed with 2 LCPs should be considered as a suitable alternative in dogs with severe shoulder joint pathology.

Identification, Quantification, and Characterization of the Phenolic Fraction of Brunfelsia grandiflora: In Vitro Antioxidant Capacity.

Brunfelsia grandiflora is an ancient plant widely used for its promising medicinal properties, although little explored scientifically. Despite being a rich source of phenolic compounds responsible in part for the proven anti-inflammatory activity, its characterization has not been carried out to date. The present work deals with the exhaustive identification and quantification of its phenolic fraction, along with its antioxidant activity. Decoction resulting from the bark as fine powder was filtered and lyophilized, and polyphenols were extracted from the resulting product by aqueous-organic solvents. Seventy-nine polyphenols were identified using LC-MSn. Hydroxycinnamates was the most abundant group of compounds (up to 66.8%), followed by hydroxycoumarins (15.5%), lignans (6.1%), flavonols (5.7%), phenolic simples (3.1), gallates (2.3%), flavanols (0.3%), and flavanones (0.2%). About 64% of the characterized phenols were in their glycosylated forms. The quantification of these phytochemicals by LC-QToF showed that this medicinal plant contained 2014.71 mg of phenolic compounds in 100 g dry matter, which evidences a great antioxidant potency determined by ABTS and DPPH assays. Therefore, Brunfelsia grandiflora represents an important source of polyphenols which supports its therapeutic properties scientifically proven.

Marginal excision of cutaneous mast cell tumors in dogs was not associated with a higher rate of complications or prolonged wound healing than marginal excision of soft tissue sarcomas.

OBJECTIVE: To compare wound healing following planned marginal excision of cutaneous mast cell tumors (MCTs) with that of soft tissue sarcomas (STSs) and to identify risk factors for wound healing complications and delay in healing. ANIMALS: 126 dogs that underwent intentional marginal excision of cutaneous MCTs (n = 77) or subcutaneous STSs (49). PROCEDURES: Medical records of included dogs were reviewed and signalment, tumor size, tumor location, skin closure type, time to healing, reported complications, histopathological grade, and surgical margins were recorded. These variables and outcomes (complication rate and time to complete healing) were compared between dogs in the MCT and STS groups. Potential risk factors for complications and delayed healing were analyzed. RESULTS: No significant difference between the groups was found in any of the variables. Wound healing complication rates were 29% (22/77) for the MCT group and 31% (15/49) for the STS group. The mean ± SD time to complete healing was 16.5 ± 7.5 days for the MCT group and 17.7 ± 9.3 days for the STS group. These outcomes did not differ significantly between groups. For both groups, the use of subdermal plexus flap reconstruction was associated with the development of complications and increased time to complete healing. CLINICAL RELEVANCE: Marginal excision of cutaneous MCTs was not associated with a higher rate of complication or prolonged wound healing, compared with marginal excision of STSs. The use of flap reconstruction in skin closure may delay healing and planned adjuvant therapy. Owners should be counseled regarding these risks and where appropriate and feasible, surgery without reconstruction should be considered.

Complications after proximal abducting ulnar osteotomy and prognostic factors in 66 dogs.

OBJECTIVE: To report complications and prognostic factors in dogs undergoing proximal abducting ulnar osteotomy (PAUL). To evaluate the ability to predict complications on the basis of post-operative radiographic examination. STUDY DESIGN: Retrospective cohort study. ANIMALS: Sixty-six dogs. METHODS: Medical records of dogs treated with PAUL between 2014 and 2019 were reviewed for demographics, intraoperative findings, and post-operative complications. Post-operative radiographs were reviewed by two masked expert orthopedic surgeons, who were asked to predict the likelihood of major mechanical complications. The prognostic value of variables was tested with univariate and multivariable logistic regression. Inter-investigator agreement to predict complications was evaluated with two-by-two tables and kappa coefficient. RESULTS: Seventy-four PAULs in 66 dogs were included. Duration of follow-up ranged from 12 to 75 months (median: 53 months). Post-operative complications were documented in 19/74 limbs (16 dogs), including major complications in 13 limbs. These complications consisted mainly of non-union (six limbs), implant failure (two limbs), and infection (two limbs) requiring revision surgery in nine limbs. Body weight was the only variable associated with an increased risk of post-operative complications (p = .04). Agreement between expert predictions was low (respectively k = -0.08 and k = 0.11). CONCLUSION: Major complications were reported in one fourth of limbs treated with PAUL and were more likely as body weight increased. Suboptimal plate and screw placement or osteotomy reduction on post-operative radiographs were poorly predictive of complications. CLINICAL SIGNIFICANCE: Complications are fairly common after PAUL, particularly in heavier dogs, and post-operative radiographic examination seems unreliable to predict those.

Procedimiento para la infusión de mitocondrias autólogas por la arteria carótida en el cerebro porcino / Procedure for autologous mitochondria infusion via the carotid artery into porcine brain

Las mitocondrias son organelas complejas que desempeñan un papel fundamental en la célula, la disfunción mitocondrial puede ocasionar daños celulares significativos o la muerte. Estudios previos han demostrado los prometedores efectos terapéuticos del trasplante de mitocondrias autólogas a un tejido cardiaco isquémico, sin embargo, pocos estudios han evaluado los efectos in vivo de la infusión de mitocondrias en el cerebro. El presente trabajo tiene como objetivo dar a conocer el procedimiento para la infusión vía carótida de mitocondrias autólogas en cerebros porcinos. Mediante esta técnica de infusión, proponemos que una administración selectiva y mínimamente invasiva es factible y puede proporcionar beneficios en el tratamiento de diversas patologías del sistema nervioso central.

Mitochondria are complex organelles that play a critical role within the cell; mitochondrial dysfunction can result in significant cell damage or death. Previous studies have demonstrated the promising therapeutic effects of autologous mitochondria transplantation into ischemic cardiac tissue; however, few studies have examined the in vivo effects of mitochondria infusion into the brain. The aim of this study is to report a procedure for carotid infusion of autologous mitochondria into porcine brains. By using this infusion technique, we propose that a selective and minimally invasive administration is feasible and may provide benefits in the treatment of various central nervous system disorders.

Bilateral locking compression plate and transcondylar screw fixation for stabilization of canine bicondylar humeral fractures.

OBJECTIVE: To report the surgical management, complications, and short-term outcomes for dogs with humeral Y-T fractures (ie, fractures affecting the medial and lateral aspect of the humeral condyle extending from the articular surface) that had been approached bilaterally (medial and lateral) and stabilized with two locking compression plates (LCP) and to investigate risk factors for major complications in the short term. STUDY DESIGN: Retrospective case series. ANIMALS: Twenty-eight client-owned dogs. METHODS: Medical records of 28 dogs that presented with 29 consecutive Y-T fractures that had been approached bilaterally, reduced, and stabilized by using a transcondylar screw (in lag or positional fashion) and two or three LCP were reviewed. All dogs had at least 6 weeks clinical follow-up, and in some cases, responses from the long-term owner-based University of Liverpool questionnaire for owners of dogs with mobility problems (Liverpool Osteoarthritis in Dogs) was obtained. RESULTS: Twenty-eight dogs (29 fractures) presented with Y-T fractures and met the inclusion criteria. The fractures were reduced and stabilized with bilateral LCP and screws. The French bulldog (n = 7) was the most commonly represented breed. Age ranged from 4 months to 9.5 years (mean, 43.53 ± 34.63 months); seven (25%) dogs were considered skeletally immature. Twenty-seven (96.4%) dogs were considered to have had resolution of lameness at 6 weeks. Major complications were registered in two (7%) dogs; one dog required surgical revision, and one dog had catastrophic complications leading to amputation of the limb. The owner-based questionnaire was available for 18 (64%) dogs. CONCLUSION: Management of canine Y-T humeral fractures stabilized with bilateral LCP and transcondylar screws led to a low complication rate with a good to excellent long-term outcome according to responses on the client questionnaire. Risk factors for major complications were not identified in our sample population. CLINICAL SIGNIFICANCE: Management of Y-T humeral fractures stabilized with bilateral LCP and transcondylar screws can lead to good to excellent short-term outcome with a low complication rate in mature and immature dogs.

Risk of infection after double locking plate and screw fixation of tibial plateau leveling osteotomies in dogs weighing greater than 50 kilograms.

OBJECTIVE: To compare surgical site infection (SSI) rates after double locking plate and screw fixation (DLP), standard locking plate and screw fixation (LP), and conventional nonlocking plate and screw fixation (NLP) in dogs weighing >50 kg undergoing tibial plateau leveling osteotomy (TPLO). STUDY DESIGN: Retrospective study (January 2003-October 2017). ANIMALS: Two hundred seventy-five dogs weighing >50 kg with cranial cruciate ligament disease treated with TPLO. METHODS: Medical records of dogs weighing >50 kg that underwent TPLO by DLP, LP, and NLP with a minimum follow-up period of 12 months were included. Data collected included signalment, details of any concurrent surgical procedure, type of implant used, use of postoperative antibiotic therapy, occurrence of perioperative complications, and presence of postoperative infection. The probability, risk difference, and relative risk of SSI were estimated for each fixation with a marginal model. RESULTS: Nonlocking plate and screw fixation was used in 114 (41.5%) dogs, LP was used in 128 (46.5%) dogs, and DLP was used in 33 (12%) dogs. Surgical site infection was diagnosed in 48 of 275 (17.5%) dogs. Postoperative antibiotic therapy was used in 74 (64.9%) dogs, 62 (48.4%) dogs, and 32 (97.0%) dogs in the NLP, LP, and DLP groups, respectively. Dogs with NLP, LP, and DLP had postoperative infection rates of 24.5%, 13.3%, and 9.1%, respectively. There were no risk differences for the three groups (Holm-adjusted P > .05). CONCLUSION: No difference in infection rates was detected between DLP, LP, or NLP for TPLO in these dogs weighing >50 kg. CLINICAL SIGNIFICANCE: Fixation of TPLO with DLP in dogs weighing >50 kg does not seem to increase the risk of SSI compared with LP and NLP.

Ventral distraction-stabilization in 5 continuous sites for the treatment of cervical spondylomyelopathy in a Great Dane.

OBJECTIVE: To report the combined use of novel and commercially available implants for ventral distraction-stabilization of 5 continuous compressive sites in a Great Dane with cervical spondylomyelopathy (CSM). STUDY DESIGN: Case report. ANIMALS: One 4-year-old male Great Dane weighing 78 kg. METHODS: A tetraparetic 4-year-old Great Dane with osseous-associated CSM (OA-CSM) with 5 sites of dorsolateral compression was presented for surgical intervention. Custom designed Fitz intervertebral traction screws (FITS) and a commercially available polyaxial spinal system were used for ventral distraction-stabilization of all lesions. Multiple neurological reevaluations were performed, with the final evaluation at 27 months postoperatively. Preoperative, immediate-postoperative, and 27 month-postoperative computed tomography (CT) images were retrospectively reviewed to compare the vertebral canal dimensions at the affected sites. RESULTS: Multilevel distraction-stabilization technique resulted in improvement of the dog's neurological function by 10 days postoperatively. The vertebral canal measurements were revealed to have improved in over half of the measured variables when the 27-month-postoperative images were compared with the preoperative CT images. Increased vertebral canal dimensions were observed immediately after surgery, and some of these measurements continued to improve by 27 months postoperatively. CONCLUSION: Ventral distraction-stabilization technique with custom-designed FITS and a polyaxial spinal system resulted in both clinical improvement and objective improvement in spinal canal measurements in an OA-CSM-affected Great Dane. CLINICAL SIGNIFICANCE: An indirect decompression technique can eliminate dynamic movements and result in regression of vertebral canal bony proliferation in dogs with OA-CSM.

Short- and long-term outcome after transcondylar screw placement to treat humeral intracondylar fissure in dogs.

OBJECTIVE: To describe the postoperative complications and long-term functional outcome after surgical management of humeral intracondylar fissure (HIF) by transcondylar screw (TCS) placement. STUDY DESIGN: Retrospective study. SAMPLE POPULATION: Nineteen dogs (26 elbows) METHODS: Medical records (2000-2009) were reviewed for dogs with a diagnosis of HIF according to results of computed tomography and treated by TCS placement at a single referral institution. Collected data included signalment, surgical technique, surgical duration, surgeon experience, use of antibiotics, and the presence and nature of any complications. Owner questionnaire, orthopedic examination, and pressure platform analysis were used to assess long-term outcome. The relationship between these variables was statistically analyzed. RESULTS: In total, 18 of 26 surgically managed elbows were diagnosed with a postoperative complication; 11 of these consisted of a surgical site infection (SSI). No risk factors for short-term complications or long-term outcome were identified. Dogs with an SSI (n = 11) were 28 times more likely to have an unsatisfactory long-term outcome than dogs without an SSI (n = 8). Symmetry indices (SI) between thoracic and pelvic limbs did not return to published normal values. There was no difference in the SI of dogs with HIF and a surgically repaired contralateral humeral condylar fracture. CONCLUSION: Placement of a TCS was commonly associated with short-term complications, affecting long-term outcome. CLINICAL SIGNIFICANCE: The high complication rate after placement of lateromedial TCS provides evidence to justify the development of alternative strategies to manage HIF.

Prevalencia y experiencia de caries dental en estudiantes según facultades de una universidad particular peruana / Prevalence and experience of dental caries in different faculty students at peruvian private university

Describir la prevalencia y experiencia de caries dental en estudiantes según facultades de la Universidad Peruana Cayetano Heredia (UPCH), Lima-Perú, en el año 2012. Material y métodos: Estudio descriptivo, observacional y retrospectivo, se trabajó con datos 2 bases de datos (Base 1: 3454 registros y Base 2: 3417 registros) provenientes de estudiantes de la UPCH proporcionados por el área administrativa de la Clínica Dental Docente de la misma institución. Las variables de estudio fueron la prevalencia y experiencia de caries dental (Índices CPOD y CPOS) según facultades, las pruebas estadísticas utilizadas fueron Chi-cuadrado y Kruskall Wallis, contando con un nivel de confianza de 95% y p<0,05, se usó el programa estadístico SPSS v. 20.0. Resultados: Se encontró asociación de la prevalencia de caries dental (p<0,01) y diferencia de promedios de Índices CPOD y CPOS (p<0.01) según facultades en la UPCH. Conclusiones: Se evidenciaron diferencias estadísticamente significativas entre la prevalencia y experiencia de caries dental en estudiantes según facultades de la Universidad Peruana Cayetano Heredia, Lima-Perú, en el año 2012...

Describe the prevalence and experience of dental caries in students on faculty at the Universidad Peruana Cayetano Heredia (UPCH), Lima, Peru, in 2012. Materials and Methods: A descriptive, observational and retrospective study, worked with records of 2 data bases (Base 1: Base 2 3454 records: 3417 records) students from UPCH provided by the administrative area of the Teaching Dental Clinic of the same institution. The variables were the prevalence and experience of dental caries (DMFT and DMFS indices) as faculties, statistical tests used were Chi-square and Kruskall Wallis, with a confidence level of 95% and p <0.05, the program was used SPSS statistical v. 20.0. Results: Association of the prevalence of dental caries (p <0.01) and mean difference of DMFT and DMFS indices (p <0.01) was found as faculties in UPCH. Conclusions: Statistically significant differences between the prevalence and experience of dental caries in students is evidenced by faculties of the Universidad Peruana Cayetano Heredia, Lima - Peru, in 2012...

Cervical Distraction-Stabilization Using an Intervertebral Spacer Screw and String-of Pearl (SOP™) Plates in 16 Dogs With Disc-Associated Wobbler Syndrome.

OBJECTIVE: To retrospectively investigate a novel surgical technique using the Fitz Intervertebral Traction Screw (FITS) combined with string-of-pearl (SOP™) locking plates for the treatment of Disc Associated Wobbler Syndrome (DAWS). STUDY DESIGN: Case series (n = 16). METHODS: Ventral approach and fenestration of the affected disc(s), nuclear extirpation, and insertion of the FITS device to the level of the dorsal annulus was followed by application of one or two 3.5 mm SOP™ plates. Autogenous cancellous bone graft was placed between adjacent vertebrae. Long-term followup (>12months) via telephone or examination was attempted for all dogs. RESULTS: Case records of 16 dogs were reviewed. All dogs had Type II disc disease with a single site in 13 dogs and multiple sites in 3 dogs. Mean ± SD age was 75 ± 38 months and body weight was 34 ± 7 kg. Fifteen of the 16 dogs had significantly improved neurologic status (P =.01) at 6 weeks. Seven of the 16 dogs were examined at 24-36 months with 6 considered normal and 1 had mild ataxia. Postoperative radiographic intervertebral distraction was significant (P = .01). Radiographic bridging was recorded in 10/16 dogs at 6 weeks and in 7 dogs available for follow-up at 5-36 months. Computed tomography in 3 dogs at 24-36 months and postmortem with histology in 1 dog confirmed bone-bridging. Complications were not considered clinically significant and included implant loosening (2 dogs, both single plates) and minor end-plate subsidence (8 dogs). CONCLUSION: Application of the FITS device in combination with 2 ventral SOP™ locking plates and autogenous cancellous bone graft was associated with excellent outcomes in dogs treated for DAWS in this small case series.

Locking plate and screw fixation after tibial plateau leveling osteotomy reduces postoperative infection rate in dogs over 50 kg.

OBJECTIVE: To assess the influence of locking plate and screw implants on postoperative infection rate in dogs >50 kg undergoing tibial plateau leveling osteotomy (TPLO). STUDY DESIGN: Retrospective case series. ANIMALS: Dogs >50 kg (n = 208) that had TPLO. METHODS: Medical records (January 2003-September 2011) were reviewed for dogs that had TPLO. Type of implant used (locking plate and screw fixation [LP] and dynamic compression plate and screw fixation [NLP]), use of postoperative antibiotics and presence of postoperative infection were recorded. Multivariate analysis was performed. RESULTS: Forty dogs (21.3%) had clinical signs compatible with postoperative infection. A positive microbiology swab was available in 16/40 cases (40%). Administration of postoperative antibiotics was associated with a lower incidence of infection (P = .006) and the use of NLP was associated with a higher incidence of infection (P = .01). CONCLUSIONS: Use of LP construct and postoperative antibiotic therapy significantly decreased infection rate in dogs >50 kg that have TPLO.

Sliding humeral osteotomy: medium-term objective outcome measures and reduction of complications with a modified technique.

OBJECTIVE: To determine mid-term complication rate associated with a modified sliding humeral osteotomy (SHO) technique and compare clinical outcome with or without focal treatment (FT). ANIMALS: Dogs (n = 46; 60 elbows) with severe or persistent lameness attributable to medial coronoid disease (MCD). METHODS: Signalment, lameness, pain score, and preoperative radiographic findings were recorded. Modified Outerbridge score and fissure/fragmentation were recorded arthroscopically. SHO was performed with technical modifications. Outcome measures included lameness score, elbow pain score, owner function assessment, and force-plate preoperatively, at 6 and 12 weeks, and 6-25 months. RESULTS: SHO was performed on 60 limbs; 22 also had FT of the medial coronoid process. Mean (±SD) dog age was 44.17 ± 33.0 months and weight range was 17.4-49.8 kg. Preoperative duration of lameness was 2.16 ± 0.69 months. Lameness improved for all limbs by 12 weeks, and resolved in 49 limbs. Significant improvements in postoperative pain scores and most owner function assessments occurred. There was no significant difference with or without FT. There were no major complications and 4.17% minor complications. Force-plate data available for 18 of 46 dogs operated unilaterally preoperatively and at last follow-up showed significant improvement in ground reaction force and reduction of asymmetry index (t-test; P < .05). CONCLUSIONS: SHO with or without focal treatment of the medial aspect of the coronoid process ameliorates lameness and pain associated with MCD at medium-term follow-up. Application technique is critical to minimize morbidity.

Identificación de células proliferativas en quistes de Taenia solium / Identification of proliferating cells in Taenia solium cysts

Los neoblastos son células totipotentes, únicas responsables de la proliferación y maduración de tejidos en platelmintos de vida libre. Células similares se han aislado en platelmintos parásitos como Echinococcus. Taenia solium causa la teniasis humana (intestinal) y la cisticercosis en humanos y cerdos. La infección del cerebro con larvas (quistes) de T. solium resulta en neurocisticercosis, hiperendémica en el Perú; su tratamiento se asocia a síntomas neurológicos graves. La capacidad proliferativa y el desarrollo de los estadios de T. solium aún no se describen, y no se ha caracterizado los neoblastos de este parásito. Se buscó células proliferativas en quistes de T. solium colectados de un cerdo infectado, que fueron identificadas al replicarse e incorporar el nucleótido bromodesoxiuridina, detectado con un anticuerpo monoclonal. Una línea celular estable de neoblastos sería útil para estudios sistemáticos in vitro sobre eficacia de drogas y sobre la biología de T. solium.

Neoblasts are totipotent cells, solely responsible for the proliferation and maturation of tissues in free-living flatworms. Similar cells have been isolated from parasitic flatworms such as Echinococcus. Taenia solium causes human taeniasis (intestinal) and cysticercosis in humans and pigs. Brain infection with larvae (cysts) of T. solium results in neurocysticercosis which is hyperendemic in Peru, and its treatment is associated with serious neurological symptoms. The proliferative capacity and development stages of T. solium have not been described and the neoblasts of this parasite have not been characterized We looked for cell proliferation in T. solium cysts collected from an infected pig, which were identified when replicating and incorporating bromodeoxyuridine nucleotide detected with a monoclonal antibody. A stable cell line of neoblasts would be useful for systematic in vitro studies on drug efficacy and the biology of T. solium.

Does radiographic arthrosis correlate with cartilage pathology in Labrador Retrievers affected by medial coronoid process disease?

OBJECTIVE: To compare radiographic elbow arthrosis with arthroscopic cartilage pathology in Labrador retrievers with elbow osteoarthritis secondary to medial coronoid process (MCP) disease. STUDY DESIGN: Retrospective epidemiological study. ANIMALS: Labrador retrievers (n = 317; 592 elbow joints). METHODS: Data were collected retrospectively (June 2007-June 2011) to identify Labrador retrievers with thoracic limb lameness and elbow pain, a complete set of elbow radiographs, and a comprehensive arthroscopic surgery report. Each radiograph was scored for osteophytosis on the anconeal process and ulnar subtrochlear sclerosis using a modification of the International Elbow Working Group (IEWG) scoring system. Elbows affected by traumatic MCP fracture, humeral condylar osteochondrosis, or ununited anconeal process were excluded. The arthroscopic report was used to generate a composite cartilage score (CCS; 0 = normal, 1 = mild, 2 = moderate, 3 = severe) for each elbow joint. Ordinal regression analysis was performed to test the relationship between radiographic arthrosis score and CCS. RESULTS: There was a significant relationship between radiographic elbow arthrosis and CCS (P < .001). Elbows with a higher radiographic score were significantly more likely to have a higher CCS than elbows with a lower radiographic score. For every month increase in age, the odds of having a higher CCS increased by 0.016 (1.6%). CONCLUSIONS: Radiographic arthrosis can be used to predict the severity of arthroscopic cartilage pathology in Labrador retrievers affected by MCP disease.