RESUMEN
The three isoforms of the adaptor protein Shc play diverse roles in cell signalling. For example, the observation of p46 Shc in the nuclei of hepatocellular carcinoma cells suggests a function quite distinct from the better characterised cytoplasmic role. Ligands responsible for the transport of various Shc isoforms into organelles such as the nucleus have yet to be reported. To identify such ligands a far western approach was used to determine the p52 Shc interactome. The Ran-GTPase nuclear transport protein was identified and found to bind to p52 Shc in vitro with low micromolar affinity. Co-immunoprecipitation, pull down and fluorescence lifetime imaging microscopy experiments in stable cells confirmed cellular interaction and nuclear localisation. The nuclear transport factor protein NTF2, which functions in cohort with Ran, was shown to form a complex with both RAN and Shc, suggesting a mechanism for Shc entry into the nucleus as part of a tertiary complex.
Asunto(s)
Núcleo Celular/metabolismo , Complejos Multiproteicos/metabolismo , Isoformas de Proteínas/metabolismo , Proteínas Adaptadoras de la Señalización Shc/metabolismo , Proteína de Unión al GTP ran/metabolismo , Animales , Far-Western Blotting , Perros , Proteína Adaptadora GRB2/genética , Proteína Adaptadora GRB2/metabolismo , Humanos , Células Jurkat , Proteínas de Transporte Nucleocitoplasmático/genética , Proteínas de Transporte Nucleocitoplasmático/metabolismo , Isoformas de Proteínas/genética , Proteínas Adaptadoras de la Señalización Shc/genética , Transducción de Señal/fisiología , Proteína de Unión al GTP ran/genéticaRESUMEN
A prospective study of 100 patients undergoing nasal surgery was done to quantitate the effects of nasal surgery on olfaction. Patients were evaluated pre- and postoperatively with regard to their subjective olfaction, and pre- and postoperative olfactograms were obtained using a simple office olfactometer. Thirty-two patients had normal olfaction during the course of study. Forty-nine improved following surgery. Eight patients had a decrease in olfaction after surgery. Two of these were related to allergic rhinitis. One patient had a total loss of olfaction following a septorhinoplasty. The authors conclude that most patients undergoing nasal surgery will have either an improvement or no change in olfaction after surgery.