RESUMEN
In this study, a virus strain designated as HY12 was isolated from cattle with a disease of high morbidity and mortality in Jilin province. Biological and physiochemical properties showed that HY12 isolates is cytopathic with an extremely high infectivity. HY12 is resistant to treatment of organic solvent and acid, and unstable at 60°C for 1 h. Electron microscopy observation revealed the virus is an approximately 22-28 nm in diameter. The complete genome sequence of HY12 consists of 7416 nucleotides, with a typical picornavirus genome organization including a 5'-untranslated region (UTR), a large single ORF encoding a polyprotein of 2176 amino acids, and a 3'-UTR. Phylogenetic analysis clustered HY12 isolates to a new serotype/genotype within the clade of enterovirus E (formerly BEV-A). Alignment analysis revealed a unique insertion of 2 amino acid residues (NF) at the C-terminal of VP1 protein between aa 825 and 826, and several rare mutations in VP1 and VP4 of HY12 isolates in relation to known bovine enterovirus (BEV) strains. This is the first report of an enterovirus E in China, which is potentially associated with an outbreak in cattle with severe respiratory and enteric diseases.
Asunto(s)
Enfermedades de los Bovinos/virología , Enteritis/veterinaria , Infecciones por Enterovirus/veterinaria , Enterovirus Bovino/genética , Infecciones del Sistema Respiratorio/veterinaria , Secuencia de Aminoácidos , Animales , Proteínas de la Cápside/química , Bovinos , Línea Celular , Enteritis/virología , Infecciones por Enterovirus/virología , Enterovirus Bovino/clasificación , Enterovirus Bovino/aislamiento & purificación , Genotipo , Datos de Secuencia Molecular , Filogenia , Infecciones del Sistema Respiratorio/virología , Análisis de Secuencia de ADNRESUMEN
Triosephosphate isomerase (TPI), a glycolytic enzyme, functions in catalyzing the interconversion of glyceraldehyde-3-phosphate and dihydroxyacetone phosphate and is generally upregulated in tumours. However, there are data suggesting loss in lymphomas. To determine its effects in chicken embryonal fibroblasts (CEF) a plasmid was constructed to allow transfection. Upon culture in a suitable medium, cells transfected with the TPI demonstrated upregulation and were significantly more susceptible to apoptosis compared to controls with decreased proliferation. These finding therefore render a novel mechanism by which CEF can be triggered to undergo death by upregulation of TPI.