Effects of oxycodone hydrochloride and dezocine on hemodynamics and levels of inflammatory factors in patients receiving gynecological laparoscopic surgery under general anesthesia

Abstract We aimed to compare the effects of oxycodone hydrochloride and dezocine on hemodynamics and inflammatory factors in patients receiving gynecological laparoscopic surgery under general anesthesia. A total of 246 patients were divided into group A and B (n=123). Hemorheology indices were recorded 5 min after anesthesia (T0), 1 min after pneumoperitoneum (T1), when position was changed 5 min after pneumoperitoneum (T2), 15 min after pneumoperitoneum (T3), 1 min (T4) and 5 min (T5) after position was restored. Visual analogue scale scores 1, 2, 6, 12, 24 and 48 h after operation were recorded. Postoperative adverse reactions and visceral pain were observed. The expression levels of inflammatory factors were detected by enzyme-linked immunosorbent assay 12 h after operation. Compared with group A, group B had higher heart rate and mean arterial pressure at T2, lower central venous pressure and cardiac output at T1-T3, and higher systemic vascular resistance at T1-T5 (P<0.05). The incidence rate of pain syndrome in group A was lower (P<0.05). Group A had lower tumor necrosis factor-alpha and interleukin-6 expression levels and higher interleukin-10 level than those of group B (P<0.05). For gynecological laparoscopic surgery, oxycodone preemptive analgesia has superior outcomes to those of dezocine

Organogold(III) Complexes Display Conditional Photoactivities: Evolving From Photodynamic into Photoactivated Chemotherapy in Response to O2 Consumption for Robust Cancer Therapy.

Photodynamic therapy (PDT) is a spatiotemporally controllable, powerful approach in combating cancers but suffers from low activity under hypoxia, whereas photoactivated chemotherapy (PACT) operates in an O2 -independent manner but compromises the ability to harness O2 for potent photosensitization. Herein we report that cyclometalated gold(III)-alkyne complexes display a PDT-to-PACT evolving photoactivity for efficient cancer treatment. On the one hand, the gold(III) complexes can act as dual photosensitizers and substrates, leading to conditional PDT activity in oxygenated condition that progresses to highly efficient PACT (ϕ up to 0.63) when O2 is depleted in solution and under cellular environment. On the other hand, the conditional PDT-to-PACT reactivity can be triggered by external photosensitizers in a similar manner in vitro and in vivo, giving additional tumor-selectivity and/or deep tissue penetration by red-light irradiation that leads to robust anticancer efficacy.

Molecular cloning and expression analysis of a cytosolic heat shock protein 70 gene from mud crab Scylla serrata.

Heat shock protein 70s (Hsp70s) play important roles in resisting environmental stresses and stimulating innate immune system. To understand the immune defense mechanisms of Scylla serrata, a full-length cytosolic Hsp70 cDNA of S. serrata (designated as SSHsp70) was obtained by reverse transcriptase-polymerase chain reaction (RT-PCR) coupled with rapid amplification of cDNA ends (RACE). The full-length of SSHsp70 cDNA was 2235 bp, with a 5' untranslated region of 105 bp, a 3' untranslated region of 174 bp, and an open reading frame of 1956 bp encoding a polypeptide of 651 amino acids with an estimated molecular mass of 71.3 kDa and an estimated isoelectric point of 5.55. The cloned SSHsp70 belonged to a cytosolic Hsp70 family. Three typical Hsp70 signature motifs were detected in SSHsp70 by InterPro analysis. Quantitative PCR (qPCR) was used to detect tissue distribution and mRNA expression levels of SSHsp70 under different stress conditions. The obviously high levels of SSHsp70 transcript were in hemocyte, heart, hepatopancreas and gill, whereas low levels were detected in muscle, eyestalk, stomach, and gut. In different temperature treatments, the expression levels of SSHsp70 in low or high temperatures were higher than those in temperate temperature. In pathogen challenge treatments, the mRNA expression level of SSHsp70 reached a maximum level after 18 h and then dropped progressively. In different salt concentration treatments, the mRNA expression level of SSHsp70 had a minimum level at 25‰ salt concentration and high expression levels at high or low salt concentration. In different nitrite concentration treatments, the mRNA expression level of SSHsp70 increased progressively with the increase of nitrite concentration. The results confirmed Hsp70 could be used as a tool for evolution and phylogenetic analysis, a kind of potential biomarker, and a disease resistance factor used in application.