RESUMEN
Full-length sequences of HLA-B*39:05:01 and B*39:38Q , confirmed by cloning and sequencing in Chinese donors.
Asunto(s)
Alelos , Secuencia de Bases , Exones , Antígeno HLA-B39/genética , Mutagénesis Insercional , Eliminación de Secuencia , Pueblo Asiatico , Clonación Molecular , Codón/química , Expresión Génica , Genotipo , Antígeno HLA-B39/inmunología , Trasplante de Células Madre Hematopoyéticas , Prueba de Histocompatibilidad , Humanos , Intrones , Reacción en Cadena de la Polimerasa , Alineación de Secuencia , Análisis de Secuencia de ADN , Donantes de TejidosRESUMEN
The new HLA-B*39:01:01:04 allele differs from HLA-B*39:01:01 by a C â T substitution in intron 1.
Asunto(s)
Alelos , Antígeno HLA-B39/genética , Intrones , Polimorfismo de Nucleótido Simple , Receptores de Trasplantes , Pueblo Asiatico , Secuencia de Bases , Codón/química , Exones , Expresión Génica , Genotipo , Antígeno HLA-B39/inmunología , Trasplante de Células Madre Hematopoyéticas , Prueba de Histocompatibilidad , Humanos , Reacción en Cadena de la Polimerasa , Alineación de Secuencia , Análisis de Secuencia de ADN , Inmunodeficiencia Combinada Grave/patología , Inmunodeficiencia Combinada Grave/terapiaRESUMEN
HLA-B*39:97N differs from HLA-B*39:01:01 by a two nucleotide insertion at position 604-605.
Asunto(s)
Alelos , Codón sin Sentido , Exones , Mutación del Sistema de Lectura , Antígeno HLA-B39/genética , Donantes de Tejidos , Secuencia de Bases , Trasplante de Médula Ósea , Expresión Génica , Genoma Humano , Antígeno HLA-B39/inmunología , Prueba de Histocompatibilidad , Humanos , Reacción en Cadena de la Polimerasa , Alineación de Secuencia , Análisis de Secuencia de ADNRESUMEN
HLA-B*39:01:23 differs from HLA-B*39:01:01 by a single nucleotide substitution at position 153 C > T.
Asunto(s)
Alelos , Exones , Antígeno HLA-B39/genética , Polimorfismo de Nucleótido Simple , Donantes de Tejidos , Secuencia de Bases , Trasplante de Médula Ósea , Codón/química , Expresión Génica , Genoma Humano , Antígeno HLA-B39/inmunología , Prueba de Histocompatibilidad , Humanos , Intrones , Reacción en Cadena de la Polimerasa , Alineación de Secuencia , Análisis de Secuencia de ADNRESUMEN
B*38:01 and B*39:06 are present with phenotypic frequencies <2% in the general population, but are of interest as B*39:06 is the B allele most associated with type 1 diabetes susceptibility and 38:01 is most protective. A previous study derived putative main anchor motifs for both alleles based on peptide elution data. The present study has utilized panels of single amino acid substitution peptide libraries to derive detailed quantitative motifs accounting for both primary and secondary influences on peptide binding. From these analyses, both alleles were confirmed to utilize the canonical position 2/C-terminus main anchor spacing. B*38:01 preferentially bound peptides with the positively charged or polar residues H, R, and Q in position 2 and the large hydrophobic residues I, F, L, W, and M at the C-terminus. B*39:06 had a similar preference for R in position 2, but also well-tolerated M, Q, and K. A more dramatic contrast between the two alleles was noted at the C-terminus, where the specificity of B*39:06 was clearly for small residues, with A as most preferred, followed by G, V, S, T, and I. Detailed position-by-position and residue-by-residue coefficient values were generated from the panels to provide detailed quantitative B*38:01 and B*39:06 motifs. It is hoped that these detailed motifs will facilitate the identification of T cell epitopes recognized in the context of two class I alleles associated with dramatically different dispositions towards type 1 diabetes, offering potential avenues for the investigation of the role of CD8 T cells in this disease.