Response of food waste anaerobic digestion to the dimensions of micron-biochar under 30 g VS/L organic loading rate: Focus on gas production and microbial community structure.

Biochar modification is an effective approach to enhance its ability to promote anaerobic digestion (AD). Focusing on the physical properties of biochar, the impact of different particle sizes of biochar on AD of food waste (FW) at high organic loading rate (OLR) was investigated. Four biochar with different sizes (40-200 mesh) were prepared and used in AD systems at OLR 30 g VS/L. The research results found that biochar with a volume particle size of 102 µm (RBC-P140) had top-performance in promoting cumulative methane production, increasing by 13.20% compared to the control group. The analysis results of the variety in volatile acids and alkalinity in the system did not show a correlation with the size of biochar, but small size has the potential to improve the environmental tolerance of the system to high acidity. Microbial community analysis showed that the abundance of aceticlastic methanogen and the composition of zoogloea were optimized through relatively small-sized biochar. Through revealing the effect of biochar particle size on AD system at high OLR, this work provided theoretical guidance for regulating fermentation systems using biochar.

Cultural Considerations for Patient and Community Education in Global Health: A Qualitative Study in Lesotho.

Background: In global health, international nongovernmental organizations (NGOs) frequently hire, train, and partner with host-country clinicians who manage public outreach and patient care. Purpose and Research Design: We conducted a general interpretivist study of Basotho clinicians hired by NGOs and academic affiliates in Lesotho to identify cultural barriers and facilitators to community and patient education. Data Collection and Analysis: We conducted 13 interviews involving 16 participants (one physician, one nutritionist, 14 nurses). Using an inductive and iterative approach, we analyzed interview transcripts through the lens of social cognitive theory and identified 15 themes. Results: Major findings highlighted: 1) patient and community learners may view Basotho clinicians as authority figures; 2) family and community power dynamics affect healthcare access for vulnerable patient groups; and 3) village leaders may refuse community education when excluded from problem-solving and early planning. Conclusions: Although local clinicians and community members may identify with the same cultural group, clinicians can encounter cultural barriers to patient and community education.

Death by compaction in a robotic dumpster.

Robotic waste management automation systems are increasingly utilized around the globe as a user-friendly, ecological and hygienic alternative to waste disposal, simultaneously reducing the volume and frequency of waste collection. The present paper aims to report a case of death by compaction in an automated robotic waste management system and review relevant literature on compactor-related fatalities. The unusual fatal accident involved a 37-year-old male citizen who deliberately entered the robotic dumpster to retrieve his accidentally discarded medication. Upon entering the robotic chamber, the compaction system was automatically activated, although all safety measures were in full operation. This paper highlights the lack of standardized reporting on compactor-related mortality, which however is of importance both in terms of its social impact and public safety. These deaths occur rarely, in scattered areas of the globe, associated with various situations (e.g., occupational fatalities, homeless people, infanticide) and trash compactors of different types, technology, age, maintenance quality, warning signage and safety interlocks. In all previously reported fatalities, the technology involved was considerably older (as in conventional waste containers, garbage compaction trucks, chute-fed compactors in buildings, etc.). The case described in the present report is a rare event in both literature and international news coverage as it encompasses a fully automated technology intended for unsupervised, direct use by the public being located in public spaces where all citizens can interact with it. The aim of this report is to raise awareness and improve knowledge about safety parameters so that similar incidents can be avoided in the future.

Avaliação do potencial de valorização dos resíduos orgânicos da cidade de São Roque - SP / Evaluation of the potential for valorization of organic waste in the City of São Roque - SP

Dentre os serviços de limpeza pública destaca-se a grande quantidade de resíduos orgânicos (resíduos verdes e de feira livre) resultantes da manutenção e conservação de áreas verdes e da arborização urbana como galhos, gravetos, troncos, cascas, folhas, aparas de capina e roçada de áreas públicas, assim como aqueles provenientes das sobras de feiras livres como frutas, legumes, verduras além de carcaças de peixes, moluscos e crustáceos. Estes resíduos representam uma biomassa preciosa e de composição variada, que podem ter uma destinação mais nobre ao invés de gerar altos custos e ocupar espaço nos sistemas de disposição final dos municípios. A solução para esse problema pode ser um conjunto variado de alternativas de valorização, que merecem um estudo na área. Nesse contexto, o presente estudo teve por objetivo caracterizar e apontar os caminhos de valorização dos resíduos orgânicos no município de São Roque - SP. Para tanto, foi feita a seleção de áreas para coleta dos resíduos verdes e de feira livre gerados in situ. A intenção foi caracterizar estes resíduos para buscar a solução mais adequada de valorização. Além disso, foi feita uma busca em literatura para apoiar nesta etapa de encontro dos melhores recursos tecnológicos e obter uma resposta sustentável. Os resultados obtidos sustentam uma reflexão do atual panorama dos resíduos orgânicos, sua geração e tratamento, assim como o compromisso em relação à sua destinação e valorização. Portanto, com um desfecho satisfatório, a compostagem por leiras estáticas aeradas demonstrou possuir um desenvolvimento que atende melhor às atuais necessidades da cidade em relação às outras tecnologias estudadas. Com características e controles necessários para a produção de um composto de qualidade, dentro dos padrões exigidos por lei e nas condições de tempo e área demandadas pelo município de São Roque - SP, sendo uma solução viável para vencer esse desafio na gestão dos RSU localmente e assim contribuir na edificação de cidades mais sustentáveis pelo Brasil.

Among the public cleaning services, the large amount of organic waste (green waste and free fair waste) resulting from the maintenance and conservation of green areas and urban afforestation stands out, such as branches, sticks, trunks, bark, leaves, weeding and mowing from public areas, as well as those from leftovers from free fairs such as fruits, vegetables, greens, as well as carcasses of fish, molluscs and crustaceans. These residues represent a precious biomass with varied composition, which can have a more noble destination instead of generating high costs and occupying space in the municipal final disposal systems. The solution to this problem can be a varied set of valuation alternatives, which deserve a study in the area. In this context, the present study aimed to characterize and point out the ways of valuing organic waste in the municipality of São Roque - SP. For this purpose, the selection of areas for the collection of green waste and open-air waste generated in situ was made. The intention was to characterize these residues in order to find the most appropriate recovery solution. In addition, a literature search was carried out to support this step in finding the best technological resources and obtaining a sustainable response. The results obtained support a reflection on the current panorama of organic waste, its generation and treatment, as well as the commitment in relation to its destination and valorization. Therefore, with a satisfactory outcome, composting using aerated static windrows demonstrated a development that better meets the current needs of the city in relation to the other technologies studied. With characteristics and controls necessary for the production of a quality compost, within the standards required by law and in the conditions of time and area demanded by the municipality of São Roque - SP, being a viable solution to overcome this challenge in the management of MSW locally and thus contribute to the construction of more sustainable cities in Brazil.

Evaluation of nutritional composition of flour residue of mangaba processing / Avaliação dos fatos nutricionais do processamento do resíduo da farinha de mangaba

Among several fruits, mangaba (Hancornia speciosa Gomes), it aroused the interest of producers and consumers due to its attractive sensory characteristics and health beneficial properties (high nutritional value and presence of bioactive substances), thus, this work evaluates the nutritional factors of the flour residue of mangaba processing that is despised by the food industry, and the influence of temperature on its production. The mangaba processing residue was splited in two main groups: in natura sample (control), and other for preparation of flour that was dried at 50 °C and divided into two other groups: treatment A (flour with roasts at 110 °C and 130 °C) and treatment B (flour from drying at 50 °C). The nutritional characteristics of flours were analyzed considering the chemical parameters: pH, titratable total acidity and soluble solids, in addition to the determination of moisture content, total lipids, total dietary fiber and ash, total energy value, antioxidant activity, phytochemical screening, quantification of phenolic compounds and flavonoids, as well as technological functional properties (water absorption index (WAI), water solubility index (WSI), milk absorption index (MAI) and milk solubility index (MSI) and oil absorption index (OAI). The results showed that the bioactive compounds present in the extracts do not have significant properties of acting as free radical kidnappers. The heat treatment, performed in the flour of mangaba processing residues, influenced the nutritional factors and properties of absorption and solubility, which showed statistical differences. These results show that the flour is a viable alternative for the energy enrichment of diets, contributing to the development of new products, the reduction of the disposal of these residues and consequently to the minimization of the environmental impact.

Dentre as diversas frutas a mangaba (Hancornia speciosa Gomes), despertou interesse de produtores e consumidores devido às suas características sensoriais atrativas e propriedades benéficas à saúde (elevado valor nutricional e presença de substâncias bioativas), assim, o trabalho avaliar os fatores nutricionais do resíduo da farinha de processamento de mangaba que é desprezado pela indústria alimentícia e, a influência da temperatura na sua produção. O resíduo de processamento da mangaba foi dividido em dois lotes, sendo um deles utilizado para as análises in natura (amostra controle) e o outro para a confecção da farinha que foi seca a 50 °C, e dividida em dois lotes: tratamento A (farinha com torras a 110 °C e 130 °C) e no tratamento B (farinha oriunda da secagem a 50 °C). Analisou-se as características nutricionais de farinhas considerando os parâmetros químicos: pH, acidez total titulável e sólidos solúveis, além da determinação do teor de umidade, lipídios totais, fibra alimentar total e cinzas, valor energético total, atividade antioxidante, triagem fitoquímica, quantificação de compostos fenólicos e flavonoides, bem como as propriedades funcionais tecnológicas (índice de absorção de água (IAA), índice de solubilidade em água (ISA), índice de absorção de leite (IAL) e índice de solubilidade em leite (ISL) e índice de absorção de óleo (IAO). Na análise foi inferido que os compostos bioativos presentes nos extratos não possuem propriedades significativas de agir como sequestradores de radicais livres. O tratamento térmico, realizado na farinha de resíduos de processamento de mangaba, influenciou nos dados dos fatores nutricionais e das propriedades de absorção e solubilidade, os quais apresentaram diferenças estatísticas. Estes resultados credenciam a farinha como uma alternativa viável para o enriquecimento energético de dietas, contribuindo para o desenvolvimento de [...].

Optimized biotransformation of acid-treated water melon peel hydrolyzate into ethanol / Biotransformação otimizada de hidrolisado de casca de melancia tratada com ácido em etanol

Today, global focus of research is to explore the solution of energy crisis and environmental pollution. Like other agricultural countries, bulk quantities of watermelon peels (WMP) are disposed-off in environment as waste in Pakistan and appropriate management of this waste is the need of hour to save environment from pollution. The work emphasizes the role of ethanologenic yeasts to utilize significant sugars present in WMP for low-cost bioethanol fermentation. Dilute hydrochloric acid hydrolysis of WMP was carried out on optimized conditions employing RSM (response surface methodology) following central composite design (CCD). This experimental design is based on optimization of ethanologenesis involving some key independent parameters such as WMP hydrolysate and synthetic media ratio (X1), incubation temperature (X2) and incubation temperature (X3) for maximal ethanol yield exploiting standard (Saccharomyces cerevisiae K7) as well as experimental (Metchnikowia cibodasensisY34) yeasts. The results revealed that maximal ethanol yields obtained from S. cerevisiae K7 was 0.36±0.02 g/g of reducing sugars whereas M. cibodasensisY34, yielded 0.40±0.01 g ethanol/g of reducing sugars. The yeast isolate M. cibodasensisY34 appeared as promising ethanologen and embodies prospective potential for fermentative valorization of WMP-to-bioethanol.

Hoje, o foco global da pesquisa é explorar a solução da crise energética e da poluição ambiental. Como em outros países agrícolas, grandes quantidades de cascas de melancia (WMP) são descartadas como resíduos no meio ambiente no Paquistão, mas a gestão adequada desses resíduos é a mais recente solução para salvar o meio ambiente da poluição. O trabalho enfatiza o papel das leveduras etanologênicas para utilizar açúcares significativos presentes no WMP para fermentação de bioetanol de baixo custo. A hidrólise de ácido clorídrico diluído de WMP foi realizada em condições otimizadas empregando RSM (metodologia de superfície de resposta) e seguindo o projeto de composto central (CCD). Este projeto experimental é baseado na otimização da etanologenesis envolvendo alguns parâmetros independentes importantes, como hidrolisado de WMP e razão de meio sintético (X1), temperatura de incubação (X2) e temperatura de incubação (X3) para rendimento máximo de etanol explorando o padrão (Saccharomyces cerevisiae K7) também como leveduras experimentais (Metchnikowia cibodasensis Y34). Os resultados revelaram que os rendimentos máximos de etanol obtidos a partir de S. cerevisiae K7 foi de 0,36 ± 0,02 g / g de açúcares redutores, enquanto M. cibodasensis Y34 rendeu 0,40 ± 0,01 g de etanol / g de açúcares redutores. O isolado de levedura M. cibodasensis Y34 apareceu como um etanologeno promissor e incorpora um potencial prospectivo para a valorização fermentativa de WMP em bioetanol.

Obtención y caracterización fisicoquímica de un sustrato nutritivo hecho a partir de cáscaras de cacao para la producción de plántulas de hortalizas / Obtaining and physicochemical characterization of a nutritive substrate made from cacao shells for the production of vegetable seedlings

En el beneficiado del cacao se producen una gran cantidad de residuos, las mazorcas generalmente son desechadas dentro de los mismos cultivos y genera problemáticas como la proliferación de insectos y microorganismos patógenos. De estos desechos las cáscaras son las de mayor relevancia. La Escuela de Ingeniería Química de ITCA-FEPADE evaluó añadirle valor a este subproducto, incorporando desechos de cáscara de cacao en la formulación de un sustrato para el cultivo de plántulas de hortalizas. El objetivo de esta investigación fue obtener un sustrato orgánico que sirva de soporte material y nutritivo a partir de cáscaras de cacao criollo. La biomasa vegetal se caracterizó teniendo en cuenta parámetros como el porcentaje de humedad, pH, porcentaje de cenizas, contenido de potasio, nitrógeno y fósforo. Se ejecutaron pruebas comparativas de formulación del sustrato, siembra, cultivo y crecimiento de las plántulas de hortalizas de tomate y pepino, obteniendo como resultado la fórmula óptima de un sustrato y abono orgánico y el mejor medio de desarrollo. De los resultados obtenidos se concluye que, a partir de un adecuado procesamiento y aprovechamiento de las cáscaras de cacao, se puede transformar este desecho en un producto biomaterial alternativo que genera una opción de bioprospección agroindustrial. Los valores obtenidos en la caracterización fisicoquímica de las cáscaras de las mazorcas, dependen de condiciones como el tipo de suelo, variables agrometeorológicas, calidad de agua, abono y especie de la planta de cacao. El escenario de siembra condiciona el desarrollo óptimo de las plántulas; los factores como requerimiento de agua, distribución de nutrientes y estabilidad de las plántulas, se ven afectados por la relación del espacio de germinación. Como resultado de la caracterización fisicoquímica, se obtuvo: pH de 5.7, cenizas 18.83%, humedad 73.56%, celulosa 21.39%, lignina 39.81%, nitrógeno total 0.02%, fósforo total 0.02% y ausencia de potasio.

In the cacao beneficiation, a large amount of waste is produced, generally, the cacao pods are discarded within the same crops and generate problems such as the proliferation of insects and pathogenic microorganisms. The shell are the most relevant this waste. In Escuela de Ingeniería Química of ITCA-FEPADE the incorporation of cacao shell waste in the formulation of a substrate for growing vegetables was evaluated because the giving benefit to these by-products. The objective of this research was to obtain a substrate that serves as material and nutritional support for vegetable seedlings, from creole cacao shells. The vegetal biomass was characterized taking into account parameters such as moisture percentage, pH, ash percentage, potassium, nitrogen and phosphorus content. Subsequently, comparative tests of formulation, planting and growth of vegetable tomato and cucumber seedlings were carry out, obtaining as result, the optimal formula for the substrate and the best development environment. It concludes that, through an adequate processing and use of the cacao shell, it is possible to transform a waste into an alternative biomaterial product that generates an agro industrial bioprospecting option. The values obtained in the physicochemical characterization of the shells of cacao pods depend on conditions such as soil type, agrometeorological variables, water quality, fertilizer and cacao plant species. The planting environment determines the optimal development of the seedlings, factors such as water requirement, nutrient distribution and seedling stability seems affected because relation of the germination space. The results of the characterization were a pH of 5.7, ashes 18.83%, humidity 73.56%, cellulose 21.39%, lignin 39.81%, total nitrogen 0.02%, total phosphorus 0.02%, and total phosphorus 0.02% and an absence of potassium.

Different Educational Interventions on Individual Cognition of Garbage Classification Based on EEG Monitoring.

Improvement in an individuals' cognition is the key to promote garbage classification. This study takes university students as the research subjects, through three educational interventions, including the self-learning, heuristic learning, and interactive learning ways, to seek the most effective intervention based upon event-related potentials (ERPs) that is beneficial to enhance cognition of garbage classification. The results show that the experimental subjects induced P300 and LPP components, representing attentional changes and cognitive conflicts in classification judgments. There are differences in the amplitudes and peak latency of the two components corresponding to different interventions, indicating that the three educational interventions are able to improve the individual's cognition level of garbage classification within a certain period of time. The interactive-learning intervention triggers the largest amplitudes of P300 and LPP, as well as the smallest peak latency, indicating its effect is the best. Such results provide insight into the design for an appropriate strategy in garbage classification education. The study also shows that an EEG signal can be used as the endogenous neural indicator to measure the performance of garbage classification under different educational interventions.

Artificial Intelligence-Based Robotic Technique for Reusable Waste Materials.

Waste management is a critical problem for every country, whether it is developed or developing. Selecting and managing waste are a critical part of preserving the environment and maximizing resource efficiency. In addition to reducing trash and disposal, reusable items are predicted to be of great benefit since they lessen our dependence on raw materials. The usage of compostable trash may be expanded outside fertilizers and dung after the metallic, chemicals, and glass items have been recycled. After a good scrubbing, the glass may be broken down and remelted to create new items. Reusing waste items via garbage recovery is one of the best methods to do so. This document outlines the steps that must be taken to maximize the use of garbage. This work describes a reusable industrial robot arm for grasping and sorting things depending on the resources they contain. Gripping, motion control, and object material categorization are all integrated into a full-automation, reusable system architecture in this study. LeNet also was adjusted to classify garbage into cartons and plastics using an artificial intelligent technique, with the use of a customized LeNet model. Movement in terms of moving the robot in the most efficient way possible, the robot's grabbing, and categorization were incorporated into the movement design process. The system's grabbing and object categorization success rates and computation time are calculated as metrics for evaluation.

Production of proline and protease with different organic wastes in bacteria (Production proline and protease with organic wastes) / Produção de prolina e protease com diferentes resíduos orgânicos em bactérias (produção de prolina e protease com resíduos orgânicos)

In this study, we investigated the proline and protease production of different bacteria in several organic waste materials. Our aim was to produce proline and protease economically in waste that is abundantly available while reducing its environmental impact. 5 ml of different organic waste materials (OWW: Olive waste water; N.B: Nutrient Broth; EW: Eggshell; PBS: PBS buffer; PLW: Peach leaf wastes; TCW: Turkish coffee wastes; TWW: Tea waste water; WCW: Waste cheese whey; WFO: Waste frying oil) were placed in 10 ml grow tubes, inoculated and incubated for 24 h. Phosphate-buffered saline and 10% solutions of different organic wastes were added. These cultures were subsequently incubated at 37°C for 24 h. Cells were harvested at 24 h for L-proline assay. 1 ml of culture was transferred by pipette into an Eppendorf tube and centrifuged at 14,000 rpm for 20 min at room temperature. Cellular debris was removed by centrifuge and the supernatant was used for proline activity assays. Protease activity was determined using a modified method with casein as the substrate. We found that proline and protease can easily be produced economically using Turkish coffee wastes (TCW), Waste cheese whey (WCW) and Olive waste water (OWW) organic waste. We believe that this study will result in similar research leading to the economical use of these waste materials thus reducing their impact on the environment.

Neste estudo, investigamos a produção de prolina e protease de diferentes bactérias em diversos resíduos orgânicos. Nosso objetivo era produzir prolina e protease economicamente em resíduos que estão disponíveis em abundância, reduzindo seu impacto ambiental. Cinco ml de diferentes materiais de resíduos orgânicos (OWW: resíduos de azeitona; NB: caldo nutriente; EW: casca de ovo; PBS: tampão PBS; PLW: resíduos de folhas de pêssego; TCW: resíduos de café turco; TWW: resíduos de chá; WCW: resíduos de queijo soro de leite; WFO: óleo de fritura residual) foram colocados em tubos de cultivo de 10 ml, inoculados e incubados por 24 horas. Adicionaram-se solução salina tamponada com fosfato e soluções a 10% de diferentes resíduos orgânicos. Essas culturas foram subsequentemente incubadas a 37° C durante 24 h. As células foram colhidas às 24 h para o ensaio de L-prolina. Um ml de cultura foi transferido por pipeta para um tubo Eppendorf e centrifugado a 14.000 rpm, por 20 min, em temperatura ambiente. Os detritos celulares foram removidos por centrifugação e o sobrenadante foi usado para ensaios de atividade de prolina. A atividade da protease foi determinada usando um método modificado com caseína como substrato. Descobrimos que a prolina e a protease podem ser facilmente produzidas economicamente, usando resíduos de café turco (TCW), resíduos de soro de queijo (WCW) e resíduos orgânicos de água de oliva (OWW). Acreditamos que este estudo resultará em pesquisas semelhantes, levando ao uso econômico desses materiais residuais, reduzindo, assim, seu impacto no meio ambiente.

Cuantificación del desperdicio de alimentos en servicios de alimentación de la Universidad de Costa Rica / Quantification of Food Waste in Food Services of the Universidad de Costa Rica

Resumen Antecedentes: los alimentos desperdiciados en ventas al detalle en Latinoamérica (15 %) podrían suplir las necesidades alimenticias del 64 % de quienes sufren hambre en la región. Objetivo: determinar los desperdicios de alimentos en tres servicios de alimentación de la sede Rodrigo Facio de la Universidad de Costa Rica a partir de la guía de medición de la Red Costarricense para Disminuir la Pérdida y el Desperdicio de Alimentos. Materiales y métodos: los desperdicios en los servicios de alimentación N1 y N2 fueron medidos en 2018 y 2019 y en el N3 en 2019; se realizaron 15 mediciones siguiendo la metodología de la guía y se reportaron como porcentaje del total de producción de sólidos. Resultados: los desperdicios en la producción total fueron 15,0-16,6 % en 2018 y 6,4-11,5 % en 2019. No se encontraron diferencias entre los servicios de alimentación en la producción promedio (p=0,1467), en el desperdicio del área de almacenamiento (p=0,1293) ni en la producción de alimentos (p=0,7718). Se encontraron diferencias en el desperdicio entre los servicios de alimentación en el área de servicio: desperdicios en barra (p=0,0197), en plato/ desayuno (p=0,0075) y en plato/almuerzo (p=0,0362). Conclusiones: la cantidad de desperdicios encontrados son menores a los reportados en otros estudios.

Abstract Background: Food wasted in retail in Latinamerica (15%) could supply the nutritional needs of 64% of the hungry in the region. Objective: To determine food waste in three food services of the Rodrigo Facio campus of the Universidad de Costa Rica, using the measurement guide of the Costa Rican Network to reduce food loss and waste. Materials and Methods: Waste in food services N1 and N2 were measured in 2018 and 2019, and in the N3 in 2019. In total 15 measurements were made following the methodology of the guide and they were reported as a percentage of total solids production. Results: Waste in total production was 15.0-16.6% in 2018 and 6.4-11.5% in 2019. No differences were found between food services in average production (p=0.1467), in the waste storage area (p=0.1293), nor food production (p = 0.7718). Differences in waste were found between food services in the service area: Waste in the service bar (p=0.0197), in plate/breakfast (p=0.0075), and in plate/lunch (p=0.0362). Conclusions: The amount of waste found is lower than that reported in other studies.

Antioxidant capacity, reproductive response and glucose concentration and insulin of ewes supplemented prior to insemination with orange residue / [Capacidade antioxidante, resposta reprodutiva e concentração de glicose e insulina de ovelhas suplementadas antes da inseminação com resíduo de laranja]

The objective was to evaluate the antioxidant capacity, glucose and insulin concentration and reproductive performance of ewes supplemented with orange residue prior to insemination. Fifty-five multiparous ewes were divided into two corrals, and 15 unbred ewes were kept individually to measure feed consumption. Two integral diets were administered; T0: control treatment and T1: with 20% of dry matter of orange residue. Ten days after the start of supplementation, the ewes were synchronized. Supplementation was finalized prior to artificial insemination, then, a blood sample was taken to measure the antioxidant capacity and glucose and insulin concentration. An analysis of variance was made to evaluate the effect of treatment on the antioxidant capacity, glucose and insulin; and to analyze the response to estrus, percentage of gestation and prolificity a ji squared test was performed. Of 9 antioxidant compounds found in the orange residue, hesperidin (7.44%), chlorogenic acid (0.50%) and protocatechuic acid had the highest concentration. Feed intake, estrus response, percentage of gestation, antioxidant capacity, and glucose and insulin concentration were not affected by the treatment. It is concluded that inclusion of 20% of orange residue in the diet prior to insemination in ewes is possible.(AU)

O objetivo deste estudo foi avaliar a capacidade antioxidante, a concentração de glicose e insulina e o comportamento reprodutivo de ovelhas suplementadas com resíduo de laranja antes da inseminação. Cinquenta e cinco ovelhas multíparas foram divididas em dois currais e 15 ovelhas sem raça foram mantidas individualmente para se medir o consumo de ração. Duas dietas integrais foram administradas; T0: tratamento controle e T1: dieta com 20% de resíduo de laranja seco. Dias após o início da suplementação, as ovelhas foram sincronizadas. A suplementação foi finalizada antes da inseminação artificial e, em seguida, foi coletada uma amostra de sangue para medir a capacidade antioxidante e a concentração de glicose e insulina. Uma análise de variância foi feita para avaliar o efeito do tratamento sobre a capacidade antioxidante, a glicose e a insulina, e um teste do ji quadrado foi realizado para analisar a resposta ao estro, a porcentagem de gestação e de prolificidade. Dos nove compostos antioxidantes encontrados no resíduo laranja, a hesperidina (7,44%), o ácido clorogênico (0,50%) e o ácido protocatecuico foram os de maior concentração. O consumo alimentar, a resposta ao estro, a porcentagem de gestação, a capacidade antioxidante, a concentração de glicose e a insulina não foram afetados pelo tratamento. Conclui-se que é possível a inclusão de 20% de resíduos de laranja na dieta antes da inseminação em ovelhas.(AU)

Avaliação do resto e ingesta de refeições em um restaurante de empresa privada / Evaluation of the rest and intake and the meals in a private company restaurant

O presente estudo objetivou avaliar o índice de resto e ingesta em um restaurante de empresa privada, que possui clientela fixa. Para a mensuração desse índice, foram utilizadas 25 numerações aleatórias nos pratos dispostos na unidade de alimentação e nutrição, os quais foram pesados inicialmente e recolhidos ao final das refeições, e colocados em fórmula padrão para o cálculo desse índice. Foram observados que de acordo com a média e desvio padrão, o índice de resto e ingesta foi maior que 10%, o que mostra desperdício de alimentos nessa unidade, o que aumenta os custos ao final da produção, visto que o tempo dispendido em cada etapa da produção é somado ao custo final, sendo importante que haja ações educativas para capacitação dos colaboradores da unidade a fim de diminuir o quantitativo de refeições rejeitadas, fazendo só a quantidade suficiente para cada.(AU)

The present study aims to evaluate the rate of reinstatement and entrance to the restaurant of a private company, which has a fixed clientele. To measure this index, 25 random numbers were used in dishes arranged in the food and nutrition unit, which were weighed and collected at the end of meals, and used in the standard method for calculating the index. It was observed that the agreement with mean and standard deviation, the rest ratio and the input was greater than 10%, which shows the waste of food in this unit, which increases the costs until the final production, given the time spent in each the production stage is added to the final cost, and it is important that there are educational actions to train the unit's employees and reduce the quantitative value of rejected meals, thus making the quantity sufficient for each meal schedule.(AU)

The synergistic strategy and microbial ecology of the anaerobic co-digestion of food waste under the regulation of domestic garbage classification in China.

With the implementation of new domestic garbage classification policy in China, attention is growing to improve the treatment efficiency of municipal 'wet' waste. Combing with the new regulation, the synergistic strategy and the microbial ecology of the anaerobic co-digestion (AcoD) of cooked food waste (CFW), uncooked food waste (UCFW) and rice straw (RS) were analyzed in current study. Results showed that the maximum cumulative methane yield (CMY) and synergic index were obtained when CFW and UCFW were mixed at the ratio of 1:1 (based on volatile solid content). The highest CMY 452.94 ± 0.99 mL/g-VS was obtained when the ratio of CFW, UCFW and RS was 0.81:0.09:0.10, which was 16.29%, 36.20% and 121.84% higher than their mono-digestion, respectively. The AcoD promoted the methane potential by prolonging the release time of organic matter and slowing down the hydrolysis rate. Furthermore, the AcoD increased the species diversification and relative abundance of fermentation bacteria in digesters, and Methanosaeta predominated the methanogen communities. This study demonstrated a clean and sustainable AcoD strategy for safe disposal of urban food waste and revealed the variation of microbial community, which can provide a base for efficient bioenergy recovery from urban domestic garbage.

Parvularcula mediterranea sp. nov., isolated from marine plastic debris from Zakynthos Island, Greece.

A Gram-negative, dark orange-pigmented, aerobic, non-spore-forming, coccoid-shaped bacterium designated as ZS-1/3T was isolated from a floating plastic litter (polypropylene straw) sample, collected from shallow seawater near the public beach of Laganas on Zakynthos island, Greece. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate is affiliated with the genus Parvularcula in the family Parvularculaceae. Its closest relatives are Parvularcula lutaonensis (98.09  %) and Parvularcula oceanus (95.89  %). The pH and temperature ranges for growth are pH 5-10 and 20-38 °C (optima, pH 7.0 and 28 °C). The predominant fatty acids are C18 : 1 ω7c (56.84  %), C16 : 0 (27.51  %), C18 : 0 (2.25  %) and C12 : 0 (1.42  %). The predominant respiratory quinone detected in strain ZS-1/3T is quinone-10 (Q10); the majority of detected polar lipids are glycolipid. The DNA G+C content is 62.5  mol%. Physiological and chemotaxonomic data further confirmed the distinctiveness of strain ZS-1/3T from other members of the genus Parvularcula. Thus, strain ZS-1/3T is considered to represent a novel species of the genus, for which the name Parvularcula mediterranea. sp. nov. is proposed. The type strain is ZS-1/3T (=NCAIM B 02654T=CCM 9032T).

Isolation and Characterization of Alkaline Pectinase Productive Bacillus tropicus from Fruit and Vegetable Waste Dump Soil

Abstract Alkaline pectinase is the utmost significant industrial enzyme of the bioscouring process. By considering bio scouring of cotton, 30 microbial isolates from fruit and vegetable waste rich dump soil of Solang Valley and Vasishta (Manali, Himachal Pradesh, India) were isolated and screened for the alkaline pectinase production in the current research work. Only four isolates P3, P16, P21, and P27 were capable to produce extracellular alkaline pectinase at pH 9. Further by applying submerged fermentation, the alkaline pectinase production was quantitatively screened. The most efficient isolate was P3 identified as Bacillus tropicus, based on morphological, biochemical, and molecular characterization. Molecular characteristics confirmed by 16S rDNA sequence analysis. The nucleotide sequence of the isolate was novel with a 97% similarity index and submitted to the GenBank with accession number MK332379. The Bacillus strain selected was active at broad pH range from 8-10.5 and a temperature range from 25-50 oC. Optimum pH and temperature observed were 9 and 37 oC respectively and can be suitably used for the bio scouring process for the pretreatment of the fabrics.

Effect of Agitation on Anaerobic Co-Digestion of Swine Manure and Food Waste

Abstract The synergism of food waste associated with swine manure can provide an increase in biogas production, besides promoting greater stability in the anaerobic co-digestion process. To verify this effect, co-digestion tests were performed in two reactors, one with agitation, and the other without agitation. In both systems, gasometers were used to measure biogas production in an experiment lasting two hydraulic retention times (HRT). On each feeding day, the temperatures of the ambient and of the effluent taken from the reactors were measured, and samples of the food waste and effluent were collected to perform analysis of pH, total solids (TS), volatile solids (VS), fixed solids (FS), volatile acidity (AV), and total alkalinity (TA). In addition, the chemical oxygen demand (COD) was determined every five days, and gas composition was determined at the beginning of the second HRT. As important results, in both reactors a decrease in pH was verified due to the weakening of the buffer effect of the medium. This was due to the low alkalinity found in the food waste, causing an increase in acidity in the contents of the reactors. The volume of biogas produced was higher in the reactor with agitation, which meant an increased efficiency of the process. Finally, a low methane content was verified through chromatographic analyses, indicating a reduction in the activity of the microorganisms present in the medium. Thus, it is concluded that agitation linked to anaerobic co-digestion of swine manure with food waste exerted a positive effect on biogas production.

Valorização de resíduos orgânicos e redução de desperdício no ramo industrial de fornecimento de suprimentos alimentares para restaurantes do tipo "fast-food" / Organic waste recovery and waste reduction in the food supply industry for fast-food restaurants

Esta dissertação busca estudar formas para valorização de resíduos orgânicos (RO) por meio da técnica de digestão anaeróbia e redução de desperdício em empresa do ramo industrial alimentício responsável pelo fornecimento de suprimentos alimentares para restaurantes do tipo "fast-food". O panorama atual dos resíduos sólidos no Brasil indica que a fração orgânica representa mais da metade de sua composição, descartando como rejeito o que, nos moldes da economia circular, pode ser tratado como resíduo, sendo reinserido na cadeia produtiva e promovendo assim, grande redução dos impactos ambientais causados por sua destinação inadequada. A digestão anaeróbia consiste em viável alternativa para o aproveitamento de um valoroso subproduto dos resíduos orgânicos (RO), o metano; esse tratamento promove entalpia do resíduo e garante sua valorização de forma ampla. O resíduo abordado neste estudo consiste em itens que compõem sanduíches de rede "fast food", os quais serão avaliados de acordo com seu potencial de produção de metano; além disso, foi feita uma análise dos critérios utilizados pela empresa deste estudo para classificar o alimento como avaria, possibilitando a abertura de caminhos no quesito redução de desperdícios, tornando ínfima a quantidade destinada a aterros. Os resíduos orgânicos gerados pela empresa foram analisados em escalada de bancada identificando sua viabilidade para a produção de metano através de conjuntos eudiômetros, porém a rápida velocidade de hidrólise resultou em acidificação dos resíduos impedindo a ocorrência da metanogênese. Os resultados demonstraram a grande importância de ampliar o espectro de análises incluindo dados como relação Carbono/Nitrogênio e DQO (Demanda Química de Oxigênio) e DBO (Demanda Bioquímica de Oxigênio) para equilibrar parâmetros capazes de permitir a ocorrência de todas as etapas da biodigestão, uma vez que a estrutura disponível no desenvolvimento desta pesquisa não permitiu tal aprofundamento. Um manual de redução de desperdícios com foco nos Rs foi desenvolvido a fim de aprimorar processos de descarte que resultam hoje em grande geração de resíduos no cenário deste estudo.

This dissertation seeks to study ways to valorize organic waste (OR) through the technique of anaerobic digestion and waste reduction in a food industrial company responsible for the supply of food supplies for "fast-food" restaurants. The current scenario of solid waste in Brazil indicates that the organic fraction represents more than half of its composition, discarding as waste what, in the circular economy molds, can be treated as waste, being reinserted in the production chain and thus promoting a large reduction of environmental impacts caused by its improper disposal. Anaerobic digestion is a viable alternative for the use of a valuable by-product of organic waste (OR), the methane; this treatment promotes enthalpy of the waste and ensures its recovery in a broad way. The waste addressed in this study consists of items that make up "fast food" sandwiches, which will be evaluated according to their potential for methane production; furthermore, an analysis was made of the criteria used by the company of this study to classify the food as a malfunction, enabling the opening of ways in the waste reduction item, making the amount destined to landfills minimal. The organic waste generated by the company was analyzed in bench scale, identifying its viability for methane production through eudiometer sets, but the fast speed of hydrolysis resulted in acidification of the waste preventing the occurrence of methanogenesis. The results showed the great importance of broadening the spectrum of analyses by including data such as Carbon/Nitrogen ratio and COD (Chemical Oxygen Demand) and BOD (Biochemical Oxygen Demand) to balance parameters capable of allowing the occurrence of all stages of biodigestion, since the structure available in the development of this research did not allow such deepening. A waste reduction manual focused on the Rs was developed in order to improve disposal processes that currently result in large waste generation in the scenario of this study.