Asunto(s)
Quistes del Sistema Nervioso Central/patología , Tuber Cinereum/patología , Adolescente , Quistes del Sistema Nervioso Central/fisiopatología , Quistes del Sistema Nervioso Central/cirugía , Hamartoma/patología , Hamartoma/fisiopatología , Hamartoma/cirugía , Humanos , Enfermedades Hipotalámicas/patología , Enfermedades Hipotalámicas/fisiopatología , Enfermedades Hipotalámicas/cirugía , Masculino , Tuber Cinereum/fisiopatología , Tuber Cinereum/cirugíaRESUMEN
Progressive dysfunction of hypothalamic tuberoinfundibular dopaminergic (TIDA) neurons during normal aging is associated in the female rat with chronic hyperprolactinemia. We assessed the effectiveness of glial cell line-derived neurotrophic factor (GDNF) gene therapy to restore TIDA neuron function in senile female rats and reverse their chronic hyperprolactinemia. Young (2.5 months) and senile (29 months) rats received a bilateral intrahypothalamic injection (10(10) pfu) of either an adenoviral vector expressing the gene for beta-galactosidase; (Y-betagal and S-betagal, respectively) or a vector expressing rat GDNF (Y-GDNF and S-GDNF, respectively). Transgenic GDNF levels in supernatants of GDNF adenovector-transduced N2a neuronal cell cultures were 25+/-4 ng/ml, as determined by bioassay. In the rats, serum prolactin (PRL) was measured at regular intervals. On day 17 animals were sacrificed and neuronal nuclear antigen (NeuN) and tyrosine hydroxylase (TH) immunoreactive cells counted in the arcuate-periventricular hypothalamic region. The S-GDNF but not the S-betagal rats, showed a significant reduction in body weight. The chronic hyperprolactinemia of the senile females was significantly ameliorated in the S-GDNF rats (P<0.05) but not in the S-betagal rats. Neither age nor GDNF induced significant changes in the number of NeuN and TH neurons. We conclude that transgenic GDNF ameliorates chronic hyperprolactinemia in aging female rats, probably by restoring TIDA neuron function.
Asunto(s)
Envejecimiento/metabolismo , Terapia Genética/métodos , Factor Neurotrófico Derivado de la Línea Celular Glial/genética , Hiperprolactinemia/genética , Hiperprolactinemia/terapia , Adenoviridae/genética , Animales , Antígenos Nucleares/metabolismo , Núcleo Arqueado del Hipotálamo/citología , Núcleo Arqueado del Hipotálamo/metabolismo , Recuento de Células , Células Cultivadas , Enfermedad Crónica/terapia , Femenino , Genes Reporteros/genética , Vectores Genéticos/genética , Vectores Genéticos/farmacología , Hiperprolactinemia/metabolismo , Lactotrofos/metabolismo , Microinyecciones/métodos , Proteínas del Tejido Nervioso/metabolismo , Neuronas/citología , Neuronas/metabolismo , Prolactina/análisis , Prolactina/sangre , Prolactina/metabolismo , Ratas , Ratas Sprague-Dawley , Recuperación de la Función/genética , Resultado del Tratamiento , Tuber Cinereum/metabolismo , Tuber Cinereum/fisiopatología , Tirosina 3-Monooxigenasa/metabolismo , beta-Galactosidasa/genéticaRESUMEN
Intracranial osteolipomas and chondromas are rare benign tumors. Forty-five chondromas, mostly supratentorial, have been reported in the literature since 1981, with origins most commonly in the sellar regions. Twenty-one osteolipomas have been described to date, usually located near the tuber cinereum or the corpus callosum. The authors present a case of an osteochondrolipoma arising from the tentorium diagnosed in a pediatric patient at the age of 9 years. The case and treatment are discussed, and a review of the literature is provided.
Asunto(s)
Neoplasias Infratentoriales/diagnóstico , Lipoma/diagnóstico , Osificación Heterotópica/patología , Osteocondroma/diagnóstico , Tuber Cinereum/patología , Niño , Humanos , Neoplasias Infratentoriales/patología , Neoplasias Infratentoriales/cirugía , Lipoma/patología , Lipoma/cirugía , Imagen por Resonancia Magnética , Masculino , Osificación Heterotópica/fisiopatología , Osificación Heterotópica/cirugía , Osteocondroma/patología , Osteocondroma/cirugía , Tuber Cinereum/fisiopatología , Tuber Cinereum/cirugíaRESUMEN
The authors present a case of an ossified lipoma located at the tuber cinereum. Intracranial osteolipomas are rare lesions that occur in the region of the tuber cinereum. Almost all reported cases have been incidental autopsy findings. This report, however, involves a young girl who presented with an ovarian cyst and signs of precocious puberty, in whom a typical osteolipoma was surgically removed. This is the first case of hypothalamic osteolipoma presenting with endocrinological disturbances that has been published to date.