RESUMO
Aspergillus flavus NRRL 3357 was grown on enriched long-grain rice for 7-10 days to produce aflatoxin B1 (AFB1). The quantity of AFB1 in moldy rice was determined by thin-layer chromatography using ultraviolet light. When the dried moldy rice powder was fed to day-old Hubbard X Hubbard broiler chicks in unmedicated feed (AFB1 level 10 ppm) for 8 weeks, there was a profound reduction in weight gain and feed consumption. Chickens fed AFB1 developed severe liver damage, as determined by the concentration of hepatic microsomal cytochrome P-450 and by the activities of microsomal benzphetamine N-demethylase and serum glutamic oxalacetic transaminase. However, activated charcoal, reduced glutathione, cysteine, selenium (as sodium selenite), beta-carotene, and fisetin administered orally considerably reduced the toxicity of AFB1 in the experimental chickens.
Assuntos
Aflatoxinas/toxicidade , Ração Animal/toxicidade , Galinhas/metabolismo , Doenças das Aves Domésticas/etiologia , Aflatoxina B1 , Aflatoxinas/antagonistas & inibidores , Aflatoxinas/metabolismo , Animais , Carvão Vegetal/uso terapêutico , Cisteína/uso terapêutico , Feminino , Flavonoides/uso terapêutico , Flavonóis , Cobaias , Hepatopatias/etiologia , Hepatopatias/metabolismo , Hepatopatias/veterinária , Masculino , Doenças das Aves Domésticas/metabolismo , Coelhos , RatosRESUMO
Liver injury caused by a toxic dose of aflatoxin B1 (AFB1) (6 mg/kg, p.o.) in experimental chickens was measured by observing changes in hepatic microsomal cytochrome P-450 and the activity of microsomal benzphetamine N-demethylase and serum glutamic oxaloacetic transaminase (SGOT). However, simultaneous administration of activated charcoal, reduced glutathione (GSH), cysteine, selenium, beta-carotene or fisetin with aflatoxin B1 considerably reduced the toxic injury to liver as measured by the above parameters.