Health Risk Assessment of Exposure to Heavy Metals in Wheat Flour from Iran Markets: Application of Monte Carlo Simulation Approach.

The significance of flour in the Iranian diet underscores the need to ensure its safety from chemical pollutants. This study aimed to evaluate the potential health risks posed by certain heavy metals, such as Fe, Zn, Cu, Al, Co, Hg, Cr, Ni, Pb, and Cd, in wheat flour available in the Iranian market. A total of 248 flour samples were collected from 11 provinces in Iran during the winter of 2021. The health risks associated with heavy metals in children and adults were evaluated using USEPA health risk assessment guidance for superfund part A and Monte Carlo Simulation. The average concentration of Fe, Zn, Cu, Al, Co, Hg, Cr, Ni, Pb, and Cd, was equal to 30.62 ± 59.24, 4.94 ± 13.64, 1.24 ± 3.08, 2.85 ± 4.98, 0.03 ± 0.01, 0.12 ± 0.03, 1.42 ± 1, 0.23 ± 0.05, 1.71 ± 0.65, and 0.02 ± 0.004 mg/kg dry weight, respectively. Analysis of Fe, Cr, Al, Hg and Cr in all flour samples showed that the average concentration of these metals were greater than the standards levels set by the WHO/FAO. The results of the non-carcinogenic risks (HI) showed that the hazard index values (children: 0.969 ± 1.04, adult: 0.837 ± 0.905) of heavy metals through the consumption of flour to both study population were acceptable. The results of the carcinogenic risks (CR) based on Cd, and Pb concentration showed that the CR values from ingestion of flour to the children and adults population were 1.45 × 10-5 ± 5.08 × 10-5 and 1.26 × 10-5 ± 4.40 × 10-5, respectively. The results of Monte Carlo simulation showed that conventional deterministic health risk evaluation could overestimate risk outcomes. Likewise, Cr has 68.8% and 69.1% probability of non-carcinogenic risk to children and adult, respectively, and 80% and 79.8% probability of CR for adults and children respectively for Pb, suggesting that Cr and Pb is a priority control heavy metals. Therefore, it is recommended to continuously monitor the levels of heavy metals in wheat and its derived food products to ensure food safety.

Health risk assessments of heavy metals and trace elements exposure in the breast milk of lactating mothers in the Northeastern Iran.

Mother's breast milk is a natural and complete food for infants but can be a main source of exposure to toxic pollutants. These pollutants can negatively affect the health of the infant. Therefore, conducting biomonitoring surveys is essential to evaluate such health effects in toxicological research. This study aimed to estimate the probable health risks for infants exposed to essential and non-essential trace elements through breast milk ingestion. This descriptive-analytical, cross-sectional study was performed on 90 breastfeeding mothers referred to the health centers in Mashhad, Iran in January 2021. The health risk assessments (carcinogenic and non-carcinogenic risk) were estimated using chronic daily intake (CDI), hazard quotient (HQ), hazard index (HI), and lifetime carcinogenic risk (CR), which were recommended by the US Environmental Protection Agency (US EPA). The results of the HQ values of trace elements through ingestion exposure for arsenic (90%), copper (90%), zinc (40%), and iron (10%) exceeded the threshold of HQ, and arsenic (66.59%), copper (16.91%), and zinc (9.68%) and iron (4.57%) had the highest contribution to increasing the HI index. The average value of CR was 5. 08 × 10-3. Chromium and iron showed significant relationships (P<0.05) with education level and disease background in this study, and the concentration of chromium, iron, and zinc in the breast milk samples significantly changed during lactation stages (P<0.05). Overall, the risk of carcinogenicity through exposure to breast milk for infants was higher than the safety level of US EPA risk. Therefore, there could be a potential health risk of trace elements, particularly arsenic, copper, and zinc for infants in Mashhad, Iran through the consumption of mothers' breast milk. More efforts are required to control and reduce routes of receiving trace elements in breastfeeding mothers by the competent authorities.

The Global Burden of Viral Food-borne Diseases: A Systematic Review.

BACKGROUND: The objectives of this paper were to determine the overall number of diseases, deaths, and Disability-Adjusted Life Years (DALYs) caused by viral foodborne diseases (FBDs). An extensive search scheme was performed using several search terms; disease burden, foodborne disease, and foodborne viruses. METHODS: The obtained results were subsequently screened based on title, abstract, and, finally, full text. Relevant evidence on human food-borne virus diseases (prevalence, morbidity, and mortality) was selected. Of all viral foodborne diseases, norovirus was the most predominant one. RESULTS: The incidence rates of norovirus foodborne diseases ranged from 11 to 2,643 cases in Asia and from 418 to 9,200,000 in the USA and Europe. Norovirus had a high burden of disease Disability-Adjusted Life Years (DALYs) compared with other foodborne diseases. North America was reported as a country with a high burden of disease (DALYs = 9900) and illness costs. DISCUSSION: High variability of prevalence and incidence were observed in different regions and countries. Food-borne viruses pose a considerable burden on poor health throughout the world. CONCLUSION: We suggest the addition of foodborne viruses to the global burden of disease, and relevant evidence can be used to improve public health.

Probabilistic risk assessment of exposure to multiple metals and pesticides through consumption of fruit juice samples collected from Iranian market.

The current study assessed the risk posed to Iranian consumers by oral exposure to a mixture of 20 pesticides and six metals in 96 fruit juice (FJ) samples (3 batches × 4 brands × 8 types of FJs) collected from Iran market. Concentrations of metals and pesticides in FJs were quantified by inductively coupled plasma-optical emission spectrometry (ICP-OES) and chromatography-mass spectrometry (GC-MS), respectively. The mean concentration of all pesticides was below the maximum residue limits (MRLs) set by the European Union (EU). The calculated target hazard quotients (THQs) and total hazard index (HI) were <1.0 for all pesticides residue, indicating no risk. For the carcinogenic metals (As, Ni, and Pb), estimated incremental lifetime cancer risks (ILCRs) at the 50th and 95th centiles were respectively 4.25 × 10-5 and 5.30 × 10-5 (for As), 2.85 × 10-5 and 3.71 × 10-5 (for Ni), and 2.84 × 10-8, and 3.97 × 10-8 (for Pb), indicating no risk. At the 50th and 95th centiles, HI for non-carcinogenic metals (Cd, Hg, and Cr) was <1.0, indicating no risk. Based on sensitivity analyses of the input variables, the concentration of metals and pesticides, and the FJs ingestion rate had significant influential impacts on the calculated THQ and HI.

Impact of microencapsulated Ziziphora tenuior essential oil and orange fiber as natural-functional additives on chemical and microbial qualities of cooked beef sausage.

The aim of the current study was to investigate the suitability of Ziziphora tenuior essential oil (ZEO) as a preservative. For this purpose, the effect of free and microencapsulated ZEO, combined with orange fiber, was determined on the chemical and microbial qualities of cooked beef sausage. In this study, modified starch was used for encapsulation of essential oil, and subsequently, 0.5% ZEO and 1% orange fiber were used for preparing cooked beef sausages during 60 days of storage at 4°C. To assess the microbial quality of samples, total viable count (TVC), psychrophilic count (PSY), and lactic acid bacteria (LAB) were analyzed. Furthermore, peroxide value (PV) and thiobarbituric acid reactive substances (TBARS) were tested to examine lipid oxidation. The most components of ZEO were pulegone (47.12%), isomenthone (14.57%), and 1,8-cineole (12.84%) according to GC-MS analysis. The reducing power, DPPH radical scavenging activity, MIC, and MBC of ZEO were 16.44 (EC50), 8.36 (IC50), 0.625-2.5, and 1.25-5 mg/ml, respectively. Moreover, sausage containing 0.5% microencapsulated ZEO in combination with 1% orange fiber showed the best results with the following values (p ≤ .05): TVC (3.69 log CFU/g), PSY (3.51 log CFU/g), LAB (3.1 log CFU/g), PV (10.41 meq/kg lipid), and TBARS (3.1 mg MDA/kg). This is due to the antimicrobial and antioxidant properties of microencapsulated essential oil. Therefore, the results of the present study can be applied in the meat industries as a new natural preservation method.

Efficacy of low-fat milk and yogurt fortified with vitamin D3 on systemic inflammation in adults with abdominal obesity.

BACKGROUND: The prevalence of vitamin D deficiency is increasing globally and is associated with an increased risk of metabolic syndrome, autoimmune disease, and cardiovascular disease. Vit D deficiency is also associated with increased systemic inflammation. The current study aimed to determine the efficacy of low-fat milk and yogurt fortified with 1500 IU nano-encapsulated vitamin D, on systemic inflammation in abdominal obese participants. METHOD: This multi-center study was conducted using a 2.5-month parallel total-blind randomized clinical trial design. Two hundred and eighty nine subjects were allocated to four groups: low-fat milk fortified by 1500 IU nano-encapsulated vitamin D3 (200 mL/day). Simple milk (200 mL/day), low-fat yogurt fortified by 1500 IU nano-encapsulated vitamin D3 (150 g/day), and simple yogurt (150 g/day). RESULTS: The results showed that serum levels of neutrophils, lymphocytes, platelets and red blood cell distribution width (RDW) were significantly lower before and after the intervention in fortified dairy groups. The results showed that serum levels of neutrophils, lymphocytes, platelets, and RDW before and after intervention in the fortified dairy groups were significantly lower (p < 0.05). The values of = neutrophil to lymphocyte ratio (NLR), platelets to lymphocyte ratio, and RDW to platelets ratio (RPR) reduced significantly in the fortification group (p < 0.05). CONCLUSION: Fortification with nano-encapsulated vitamin D3 of dairy products may decrease inflammation in individuals with abdominal obesity.

The effects of consuming a low-fat yogurt fortified with nano encapsulated vitamin D on serum pro-oxidant-antioxidant balance (PAB) in adults with metabolic syndrome; a randomized control trial.

BACKGROUND AND AIM: The current study aimed to assess the effect of fortified yogurt with nano-encapsulated vitamin D on serum pro-oxidant anti-oxidant balance (PAB) in adults with or without metabolic syndrome. METHODS: In a quadruple blind clinical trial study, 139 adults with an age range of 30-50 years were randomly selected to receive either 1500 IU nano-encapsulated vitamin D fortified yogurt or placebo for ten weeks. Before and after the intervention period, blood sample was taken to determine the serum levels of vitamin D, pro-oxidant-antioxidant balance (PAB), and high-sensitivity C-reactive protein (hs-CRP). The laboratory tests were checked at baseline and at the end of the treatment. RESULTS: Serum vitamin D increased significantly, from 14.47 ± 6.07 ng/mL to 21.39 ± 6.54 ng/mL (P < 0.001) after ten weeks in the intervention group. Serum hs-CRP and PAB were significantly lower following consumption period in intervention group [1.95(0.4-8.15) g/dL vs. 1.35(0.25-3.62) g/dL; P = 0.013] and (135.19 ± 42.4 HK vs. 115.39 ± 44.69) HK; P = 0.018] respectively. There were no significant differences between the intervention and control groups regarding weight and BMI at the end of the intervention period (p > 0.05). CONCLUSION: Low-fat yogurt fortified with nano-encapsulated vitamin D was found to reduce serum PAB levels in adults with metabolic syndrome. PRACTICAL APPLICATION: The findings of the present study indicated that a low-fat yogurt fortified with 1500 IU nano-encapsulated vitamin D for ten weeks, leads to a significant reduction in serum hs-CRP and PAB concentrations highlighted the anti-inflammatory/anti-oxidative effect of vitamin D.

Nitrite and Nitrate Levels in Groundwater, Water Distribution Network, Bottled Water and Juices in Iran: A Systematic Review.

BACKGROUND: Nitrate and nitrite can get into the body through the consumption of contaminated water either directly or indirectly. The accumulation of these compounds in the body, in the long run, leads to health problems, for example, digestive disorders, cancers, and even death threats in children. The aim of this review was to investigate nitrate and nitrite pollution levels in drinking water and fruit juices in Iran. METHODS: In this review, data were collected through searching the Scientific Information Database, Science-Direct, Scopus, PubMed, Google Scholar, and Magiran databases using the keywords Nitrate, Nitrite, Drinking water, Drinking Water Resources, Juice and Iran. Finally, the location of the studies was geocoded through the Google My Maps (https://www.google.com/mymaps) software. RESULTS: Studies clearly indicated that the juices are safe in terms of nitrate. Nitrate and nitrite values were less than the national and international standards in all samples of bottled drinking water except for a few of the studies. The results of the reviewed studies also indicated that the nitrate content was higher than that written on the label in 96% of the samples, and nitrite was not labeled in 80% of them. The nitrate quantity was higher than the permissible limit in the water distribution network of Bushehr, Gilan and Mazandaran Provinces. Talesh, Ardabil, Hashtgerd, Divandareh, and Kerman cities had high nitrate levels in more than 50% of wells. CONCLUSION: Using nitrogen fertilizers and the lack of a wastewater treatment system were the main reasons for the presence of nitrate and nitrite.

The Effects of X-Ray Irradiation on Safety and Nutritional Value of Food: A Systematic Review Article.

X-ray is a non-thermal technology that has shown good efficacy in reducing pathogenic and spoilage bacteria, viruses and parasites. X-ray hygiene technology resulted in a high microbial loss in numerous food products, such as dairy products, ready-to-eat shrimp, oysters, fresh products, strawberries, shredded iceberg lettuce, and spinach leaves. Some X-ray studies on food safety have shown that X-ray is an effective technology and is also an appropriate alternative to the electron beam and gamma rays, and can be used in the food industry without side effects on human health. Besides, we reviewed the X-ray effect on the nutritional value of food. Therefore in this study, we aimed to review the available pros and cons of current studies regarding X-rays' effects on the food industry.

Phytochemical, antioxidant and antibacterial properties of Melissa officinalis and Dracocephalum moldavica essential oils.

Aromatic plants are rich in essential oils with considerable antimicrobial properties. The aim of this study was to investigate chemical composition, antimicrobial activity and antioxidant properties of Melissa officinalis and Deracocephalum moldavica essential oils (EOs). The identification of chemical constituents of the EOs was carried out using gas chromato-graphy-mass spectrometry analysis and antimicrobial activity of the EOs was evaluated by disc diffusion assay as well as determination of minimal inhibitory concentration (MIC) and minimal bactericidal concentration against four important food-borne bacteria: Salmonella typhimorium, Escherichia coli, Listeria monocytogenes and Staphylococcus aureus. Antioxidant activity of the EOs was also determined by 2,2-diphenyl-1-picrylhydrazyl, 2,2-azinobis 3-ethylbenzo thiazoline-6-sulfonic acid and ß-carotene bleaching tests. The major compounds of D. moldavica were geranial (28.52%), neral (21.21%), geraniol (19.60%), geranyl acetate (16.72%) and the major compounds of M. officinalis EO were citronellal (37.33%), thymol (11.96%), citral (10.10%) and ß-caryophyllene (7.27%). The underlying results indicated strong antimicrobial effects of the oils against tested bacteria. Staphylococcus aureus with the lowest MIC value (0.12 mg mL-1) for both EOs was the most sensitive bacterium, although, antibacterial effect of M. officinalis EO was stronger than D. moldavica. In addition, the results of the antioxidant activity showed that both EOs had notable antioxidant properties. In conclusion, both EOs are appropriate alternatives as potential sources of natural preservative agents with the aim of being applied in food industries.