Capsaicin-loaded alginate nanoparticles embedded polycaprolactone-chitosan nanofibers as a controlled drug delivery nanoplatform for anticancer activity.

Nanocarrier-based drug delivery systems have been designed into various structures that can effectively prevent cancer progression and improve the therapeutic cancer index. However, most of these delivery systems are designed to be simple nanostructures with several limitations, including low stability and burst drug release features. A nano-in-nano delivery technique is explored to address the aforementioned concerns. Accordingly, this study investigated the release behavior of a novel nanoparticles-in-nanofibers delivery system composed of capsaicin-loaded alginate nanoparticles embedded in polycaprolactone-chitosan nanofiber mats. First, alginate nanoparticles were prepared with different concentrations of cationic gemini surfactant and using nanoemulsion templates. The optimized formulation of alginate nanoparticles was utilized for loading capsaicin and exhibited a diameter of 19.42 ± 1.8 nm and encapsulation efficiency of 98.7 % ± 0.6 %. Likewise, blend polycaprolactone-chitosan nanofibers were prepared with different blend ratios of their solutions (i.e., 100:0, 80:20, 60:40) by electrospinning method. After the characterization of electrospun mats, the optimal nanofibers were employed for embedding capsaicin-loaded alginate nanoparticles. Our findings revealed that embedding capsaicin-loaded alginate nanoparticles in polycaprolactone-chitosan nanofibers, prolonged capsaicin release from 120 h to more than 500 h. Furthermore, the results of in vitro analysis demonstrated that the designed nanoplatform could effectively inhibit the proliferation of MCF-7 human breast cells while being nontoxic to human dermal fibroblasts (HDF). Collectively, the prepared nanocomposite drug delivery platform might be promising for the long-term and controlled release of capsaicin for the prevention and treatment of cancer.

Treatment with edaravone improves the structure and functional changes in the hippocampus after chronic cerebral hypoperfusion in rat.

This study aimed to find out cellular and electrophysiological effects of the edaravone (EDR) administration following induction of vascular dementia (VaD) via bilateral-carotid vessel occlusion (2VO). The rats were randomly divided into control, sham, 2VO + V (vehicle), and 2VO + EDR groups. EDR was administered once a day from day 0-28 after surgery. The passive-avoidance, Morris water-maze, and open-field tests were used for evaluation of memory, locomotor, and anxiety. The field-potential recording was used for assessment of electrophysiological properties of the hippocampus; and after sacrificing, the cerebral hemispheres were removed for stereological study and evaluation of MDA levels. The long-term potentiation (LTP), paired-pulse ratio (PPR), and input-output (I/O) curves were evaluated as indexes for long-term and short-term synaptic plasticity, and basal-synaptic transmission (BST), respectively. The 2VO led to increases in MDA level with considerable neuronal loss and decreases in the volume of the hippocampus, along with a reduction in the BST and LTP induction which was associated with a decrement in PPR and ultimate loss in memory with higher anxiety behavior. However, administration of EDR caused a decline in MDA and prevented the neural loss and volume of the hippocampus, rescued BST and LTP depression, improved memory and anxiety without any effects on PPR. Therefore, most likely through the improvement of MDA level, and the hippocampal cell number and volume, EDR leads to recovery of synaptic plasticity and behavioral performance. Because of the LTP rescue, without recovery of PPR, it is likely that the EDR improved LTP through the post-synaptic neurons.

Amine-terminated dendritic polymers as promising nanoplatform for diagnostic and therapeutic agents' modification: A review.

It is often challenging to design diagnostic and therapeutic agents that fulfill all functional requirements. So, bulk and surface modifications as a common approach for biomedical applications have been suggested. There have been considerable research interests in using nanomaterials to the prementioned methods. Among all nanomaterials, dendritic materials with three-dimensional structures, host-guest properties, and nano-polymeric dimensions have received considerable attention. Amine-terminated dendritic structures including, polyamidoamine (PAMAM), polypropyleneimine (PPI), and polyethyleneimine (PEI), have been enormously utilized in bio-modification. This review briefly described the structure of these three common dendritic polymers and their use to modify diagnostic and therapeutic agents in six major applications, including drug delivery, gene delivery, biosensor, bioimaging, tissue engineering, and antimicrobial activity. The current review covers amine-terminated dendritic polymers toxicity challenging and improvement strategies as well.

Amine-terminated dendritic polymers as a multifunctional chelating agent for heavy metal ion removals.

In this study, amine-terminated hyperbranched PAMAM (polyamidoamine) polymer (AT-HBP) was synthesized as a multifunctional chelating agent to remove two heavy metal ions (Cr(III) and Cu(II)) from the simulated wastewater solutions. The AT-HBP was characterized by Fourier transformed infrared (FTIR), dynamic light scattering (DLS), and proton nuclear magnetic resonance (1H NMR) analysis. The removal process was carried out in two different methods, centrifuged process and ultrafiltration. The concentration of heavy metal ions before and after removal was measured by inductively coupled plasma (ICP) instrument. The removal processes were evaluated by changing different parameters such as solution pH, AT-HBP dosage, and metal ion concentration. To evaluate the extend of binding of heavy metal ions in the presence of AT-HBP the presence of salt in the solution was also examined on the performance of the removal system. The overall results indicated that removal percentages higher than 98% for Cr(III) and 86% for Cu(II) were achieved for heavy metal concentrations of 100 mg/L for both removal process methods. Furthermore, the function of second generation of polypropylenimine (PPI) was compared to AT-HBP. The results reveal that the removal of Cr(III) and Cu(II) ions by AT-HBP were approximately 20% and 10% higher compared to PPI, respectively. Finally, hyperbranched dendritic polymer with lower expenses to synthesize compared to dendrimer underlined favorable properties as a multifunctional chelating agent and enhancement of ultrafiltration process for wastewater treatment. Graphical abstract.

Electrospun nanofibers comprising of silk fibroin/gelatin for drug delivery applications: Thyme essential oil and doxycycline monohydrate release study.

In this study, a nanofibrous electrospun substrate based on the silk fibroin (SF) and gelatin (GT) polymers were prepared and evaluated. The SF/GT blended solutions were prepared with various ratios of GT in formic acid and electrospun to obtain bead-free fibers. Results showed that addition of GT to SF increased nanofiber's diameter, bulk hydrophilicity, surface wettability, mass loss percentage, but decreased Young's modulus, tensile strength, and porosity of the SF/GT mats. According to the obtained results, the mat containing 10% of GT was selected as the optimized mat for further studies and loaded with thyme essential oil (TEO) and doxycycline monohydrate (DCMH) as the antibacterial agents. Release studies showed a burst release of TEO from the mat within the first 3 h, while the DCMH had a sustained release during 48 h. In comparison to the TEO-loaded mat, the DCMH-loaded one showed larger inhibition zones against Staphylococcus aureus and Klebsiella pneumoniae bacteria. Meanwhile, cellular studies using mouse fibroblast L929 cells showed excellent cell-compatibility of TEO- and DCMH-loaded mats. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1092-1103, 2018.