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1.
Rapid Commun Mass Spectrom ; 32(24): 2141-2151, 2018 Dec 30.
Artigo em Inglês | MEDLINE | ID: mdl-30198194

RESUMO

RATIONALE: Structural characterization of individual compounds in very complex mixtures is difficult to achieve. One important step in structural elucidation is understanding the mass spectrometric fragmentation mechanisms of the compounds present in such mixtures. Here, different individual compounds presumed to be present in a complex crude oil mixture have been synthesized and structurally characterized by tandem mass spectrometry (MS/MS) studies. METHODS: Model compounds with different aromatic cores and various substitutents were synthesized. Major effort has been put into producing isomerically pure compounds to better understand the fragmentation pattern. Each synthesized compound has been subjected to MSn studies using either a triple quadrupole or a linear ion trap mass spectrometer with electrospray or atmospheric pressure photoionization. The results are used to analyze individual compounds from a complex vacuum gas oil (VGO). RESULTS: The synthesized compounds and a chromatographically simplified vacuum gas oil were used for structural analysis. The major fragmentation mechanism is the benzylic cleavage of the aliphatic side chain. Each side chain can be separately removed from the aromatic core by using MSn methods. At the end of a series of fragmentations, the base aromatic core structure remains and can be chararcterized. CONCLUSIONS: By defining the fragmentation mechanism in complex oil samples it was possible to structurally characterize individual compounds present in a chromatographically simplified VGO. The compounds consist of an aromatic core with aliphatic side chains. Cleavage of all side chains can be achieved by MSn measurements, allowing characterization of the remaining core structure.

2.
NMR Biomed ; 31(2)2018 02.
Artigo em Inglês | MEDLINE | ID: mdl-29206323

RESUMO

High-resolution magic angle spinning (HR MAS) nuclear magnetic resonance (NMR) spectroscopy is increasingly being used to study metabolite levels in human breast cancer tissue, assessing, for instance, correlations with prognostic factors, survival outcome or therapeutic response. However, the impact of intratumoral heterogeneity on metabolite levels in breast tumor tissue has not been studied comprehensively. More specifically, when biopsy material is analyzed, it remains questionable whether one biopsy is representative of the entire tumor. Therefore, multi-core sampling (n = 6) of tumor tissue from three patients with breast cancer, followed by lipid (0.9- and 1.3-ppm signals) and metabolite quantification using HR MAS 1 H NMR, was performed, resulting in the quantification of 32 metabolites. The mean relative standard deviation across all metabolites for the six tumor cores sampled from each of the three tumors ranged from 0.48 to 0.74. This was considerably higher when compared with a morphologically more homogeneous tissue type, here represented by murine liver (0.16-0.20). Despite the seemingly high variability observed within the tumor tissue, a random forest classifier trained on the original sample set (training set) was, with one exception, able to correctly predict the tumor identity of an independent series of cores (test set) that were additionally sampled from the same three tumors and analyzed blindly. Moreover, significant differences between the tumors were identified using one-way analysis of variance (ANOVA), indicating that the intertumoral differences for many metabolites were larger than the intratumoral differences for these three tumors. That intertumoral differences, on average, were larger than intratumoral differences was further supported by the analysis of duplicate tissue cores from 15 additional breast tumors. In summary, despite the observed intratumoral variability, the results of the present study suggest that the analysis of one, or a few, replicates per tumor may be acceptable, and supports the feasibility of performing reliable analyses of patient tissue.


Assuntos
Neoplasias da Mama/diagnóstico por imagem , Neoplasias da Mama/metabolismo , Metabolômica , Espectroscopia de Prótons por Ressonância Magnética/métodos , Análise de Variância , Neoplasias da Mama/patologia , Feminino , Humanos , Lipídeos/química , Metaboloma , Análise de Componente Principal
3.
Anal Bioanal Chem ; 409(6): 1591-1606, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27896396

RESUMO

Metabolic perturbations resulting from excessive hepatic fat accumulation are poorly understood. Thus, in this study, leptin-deficient ob/ob mice, a mouse model of fatty liver disease, were used to investigate metabolic alterations in more detail. Metabolites were quantified in intact liver tissues of ob/ob (n = 8) and control (n = 8) mice using high-resolution magic angle spinning (HR-MAS) 1H-NMR. In addition, after demonstrating that HR-MAS 1H-NMR does not affect RNA integrity, transcriptional changes were measured by quantitative real-time PCR on RNA extracted from the same specimens after HR-MAS 1H-NMR measurements. Importantly, the gene expression changes obtained agreed with those observed by Affymetrix microarray analysis performed on RNA isolated directly from fresh-frozen tissue. In total, 40 metabolites could be assigned in the spectra and subsequently quantified. Quantification of lactate was also possible after applying a lactate-editing pulse sequence that suppresses the lipid signal, which superimposes the lactate methyl resonance at 1.3 ppm. Significant differences were detected for creatinine, glutamate, glycine, glycolate, trimethylamine-N-oxide, dimethylglycine, ADP, AMP, betaine, phenylalanine, and uridine. Furthermore, alterations in one-carbon metabolism, supported by both metabolic and transcriptional changes, were observed. These included reduced demethylation of betaine to dimethylglycine and the reduced expression of genes coding for transsulfuration pathway enzymes, which appears to preserve methionine levels, but may limit glutathione synthesis. Overall, the combined approach is advantageous as it identifies changes not only at the single gene or metabolite level but also deregulated pathways, thus providing critical insight into changes accompanying fatty liver disease. Graphical abstract A Evaluation of RNA integrity before and after HR-MAS 1H-NMR of intact mouse liver tissue. B Metabolite concentrations and gene expression levels assessed in ob/ob (steatotic) and ob/+ (control) mice using HR-MAS 1H-NMR and qRT-PCR, respectively.


Assuntos
Betaína/metabolismo , Fígado Gorduroso/genética , Fígado Gorduroso/metabolismo , Metaboloma , Espectroscopia de Prótons por Ressonância Magnética/métodos , Transcriptoma , Animais , Deleção de Genes , Ácido Láctico/metabolismo , Leptina/genética , Leptina/metabolismo , Fígado/metabolismo , Masculino , Redes e Vias Metabólicas , Metabolômica/métodos , Camundongos
4.
Polycycl Aromat Compd ; 35(2-4): 177-186, 2015 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-26823644

RESUMO

The chemical group of polycyclic aromatic compounds (PAC), including the better-known subgroup of polycyclic aromatic hydrocarbons (PAH) and the heterocyclic aromatic compounds (NSO-PAC, heterocycles), comprise several thousand individual compounds. It is hard to find a comprehensive overview in the literature of these PACs that includes a substantial amount of relevant properties. Here an attempt is made to summarize the most studied but also some less well-known PACs. In addition to basic data such as recommended names, abbreviations, CAS numbers, molecular formulas, chemical structures, and exact mono-isotopic molecular weights, physico-chemical properties taken from the literature like boiling points, vapor pressures, water solubilities, Henry's Law constants, n-octanol-water partition coefficients (log KOW), and pKa are summarized. Selected toxicological data are listed indicating carcinogenic and mutagenic activity or effects on different organisms. PAC nomenclature is a complex topic, so suggestions for practical use are made. Regarding available data, estimated (instead of measured) values should be used with caution because considerable deviations from experimentally determined values can occur. For an enhanced understanding of the behavior of single PACs in comparison with each other, some of the properties mentioned above are plotted vs. the number of rings or the degree of alkylation. Also, some physico-chemical data are correlated with different functional groups as substituents of the PAHs. This article reveals that rather little is known about the less common PACs, e.g., higher molecular weight compounds, alkylated or otherwise substituted aromatics, for instance, keto-, oxo-, amino-, nitro-, cyano-PAHs, or some heterocyclic aromatic compounds, including their derivatives. It mirrors the limited state of knowledge about the variety of PACs that do not belong to the 16 EPA PAHs.

5.
Anal Bioanal Chem ; 406(22): 5347-58, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24965162

RESUMO

The separation properties of six novel stationary phases for gas chromatography, commercially available from Sigma-Aldrich (Supelco) and based on ionic liquids (ILs), were investigated. The linear solvation energy relationship model (LSER) was used to describe the molecular interactions between these stationary phases and 30 solutes. The solutes belong to different groups of compounds, like haloalkanes, alcohols, ketones, aromatics, aliphatics, and others. A good description of different interactions, as described by the LSER model, could be achieved. The calculated values of system constants for the ionic liquid phases were compared with constants of commonly used standard phases like a 5 % phenyl/95 % dimethyl siloxane and a polyethylene glycol phase. The solute descriptors are in good agreement with those found by previous authors who have used the LSER model for 44 different ionic liquids as stationary phase. The experiments were carried out at two temperatures to evaluate the influence on the phase parameters and separation characteristics. The interactions of different functional groups with the IL phases are discussed. These novel IL phases are a promising replacement of or an addition to common polar phases. Based on the evaluated phase properties, several possibilities for applications of these novel phases are shown.

6.
Anal Chem ; 85(20): 9478-85, 2013 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-24063573

RESUMO

The high complexity of crude oil makes the use of chromatographic separation an important tool especially for sample simplification. The coupling of normal-phase high-performance liquid chromatography (HPLC) using a polar aminocyano column to a Fourier transform ion cyclotron resonance (FTICR) mass spectrometer offers the best attributes of good separation prior to ultrahigh resolution mass spectrometry (MS) detection. Atmospheric pressure laser ionization (APLI) was used as an ionization technique to analyze the nitrogen-containing aromatic compounds in a deasphalted crude oil due to its unique selectivity toward aromatic compounds and also due to its sensitivity. Two main chromatographic peaks were observed during this separation indicating a class-based separation. Mass spectra obtained from fractions were collected along the entire retention time and compared with each other to assign the unique constituents. By coupling the HPLC system directly to the FTICR mass spectrometer, comparable ion and UV chromatograms were obtained, reflecting the scan-to-scan sensitivity of the coupling system. The results show that it is possible to calculate reconstructed class chromatograms (RCC), allowing differences in class composition to be traced along the retention time. As an example, radical and protonated nitrogen species generated by APLI were detected along the retention time which enabled a differentiation between basic and nonbasic species in the same polar peak, thus overcoming the limitation of chromatographic resolution. This report represents the first online LC-FTICR MS coupling in the field of crude oil analysis.

7.
Environ Toxicol Chem ; 30(9): 2004-12, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21713971

RESUMO

Photo-oxidation is a potentially significant process in the degradation of crude oil spilled in the environment. The polycyclic aromatic sulfur heterocycles (PASHs) in an Egyptian crude oil (0.8 % sulfur) were photo-oxidized as a film on the surface of water in the presence of anthraquinone as photosensitizer under simulated solar irradiation. The polar photoproducts were characterized using negative ion electrospray ionization with time of flight mass spectrometry and, after trimethylsilylation, gas chromatography with mass spectrometry. The photoproducts identified revealed the presence of a large variety of sulfonic acids, aliphatic and aromatic acids, and alcohols. The data also give new information on the substituents of the aromatic compounds in the unexposed oil and indicate the presence of cyclohexyl substituted aromatic compounds.


Assuntos
Vazamento de Resíduos Químicos , Petróleo , Fotólise , Hidrocarbonetos Policíclicos Aromáticos/análise , Compostos de Enxofre/análise , Poluentes Químicos da Água/análise , Biodegradação Ambiental , Cromatografia Gasosa , Monitoramento Ambiental , Espectrometria de Massas , Oxirredução , Hidrocarbonetos Policíclicos Aromáticos/química , Compostos de Enxofre/química , Poluentes Químicos da Água/química
8.
Angew Chem Int Ed Engl ; 48(10): 1788-91, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19173274

RESUMO

Through different windows: One major obstacle in energy research is the complexity and variety of compounds present in crude oil. A study of different ionization methods for mass spectrometry shows that the mass spectrum very strongly depends on which method is used.

9.
Anal Bioanal Chem ; 392(5): 839-48, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18695977

RESUMO

The analysis of sulfur aromatics in vacuum gas oils (VGO) distilled from an Iranian light crude oil is discussed. The VGOs were fractionated into three boiling ranges, 390-460, 460-520, and 520-550 degrees C, and were analyzed using liquid chromatographic separation on a Pd(II)-bonded stationary phase followed by identification with electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). It was possible to detect a large number of thiophenes, including a substantial number of isomers, in the three VGO fractions. Separation on the palladium phase and inclusion of sulfur-selective derivatization makes electrospray ionization of these nonpolar compounds possible. An elemental composition can be assigned to a large number of S(1) compounds without ambiguity in the presence of abundant hydrocarbons. With an increase in boiling temperature, an increase in the size of the aromatic system and the number of side chain carbon atoms was observed. In addition, the masses of higher magnitude shifted toward larger aromatic systems with an increase in boiling range. A comparison of FT-ICR MS and comprehensive gas chromatography is also given.

10.
Anal Bioanal Chem ; 389(5): 1329-39, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17885749

RESUMO

Crude oil analysis has long been an inspiration for the development of analytical techniques. Especially mass spectrometry has flourished as a result of the challenge these extremely complex problems offer. Here an overview of different analytical methods is presented that shows different ways to analyze volatile and nonvolatile crude oil components. Focus has been placed on the use of mass spectrometry and especially the new developments that have been introduced using the emerging technique of Fourier transform ion cyclotron resonance mass spectrometry. These studies are examples of how far the development of analytical methods has come for the task of studying such complex problems.

11.
J Chromatogr A ; 1157(1-2): 376-85, 2007 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-17512940

RESUMO

Phenols and alcohols in complex petrochemical samples are derivatized to esters of ferrocene carboxylic acid in a rapid and simple reaction. These esters show several characteristic ions of high intensity in gas chromatography coupled with electron impact ionization mass spectrometry (GC-EI-MS) which can be used for the identification of the analyte. A differentiation between alcohols and phenols is possible due to the McLafferty rearrangement shown only by the alcohol esters. Selective ion monitoring of m/z 213 yields a phenol-selective chromatogram and m/z 230 an alcohol-selective chromatogram. For all iron containing fragments, the isotopic pattern of iron can be observed which enhances the reliability of the peak identification. The ferrocene esters of 11 alcohols, 20 alkylphenols (including octyl- and nonylphenol), phenylphenol, naphthol and hydroxyphenanthrene, several chloro- and all mononitrophenols were synthesized as well as the esters of pentadeutero- and two fluorinated phenols. Their fragmentation pattern under EI ionization is studied and a GC-ion trap-MS system was optimized for simultaneous use of the full scan mode and an MS/MS experiment in the same run. This provides for a very high selectivity in the detection of the esters and makes available the complete mass spectra without any additional measurements. The benefits of the simple and rapid derivatization procedure in combination with this powerful detection method are demonstrated for selected petrochemical samples. Several alkylphenols could be successfully identified in such samples and molecular information about unknown phenolic components could be easily obtained.


Assuntos
Álcoois/análise , Compostos Ferrosos/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Fenóis/análise , Ésteres , Espectroscopia de Ressonância Magnética , Metalocenos
12.
J Agric Food Chem ; 54(16): 5736-41, 2006 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-16881671

RESUMO

In this work, a simple and sensitive method for the analysis of the pesticide o-phenylphenol (OPP) on citrus fruits was developed. OPP is extracted with dichloromethane by ultrasonication and derivatized with ferrocenecarboxylic acid chloride. Using ferrocene as a label, residues of OPP are determined by gas chromatography with atomic emission detection in the iron selective mode or with mass spectrometric detection. Sample cleanup is simple and rapid and merely involves a removal of excess reagent on an alumina minicolumn. The method detection limit is 2 ng of OPP/g of fruit, and recoveries from lemon samples fortified at levels of 35 and 140 ng/g are 101 and 106%, respectively. The citrus fruits analyzed (oranges, grapefruits, lemons) contained between 60 ng/g and 0.37 microg/g OPP (RSD = 8-13%), and the results were in good agreement with results obtained when OPP was analyzed using an established HPLC-FLD method. Several alcohols could also be identified in the fruit peel.


Assuntos
Compostos de Bifenilo/análise , Cromatografia Gasosa/métodos , Citrus/química , Frutas/química , Praguicidas/análise , Agroquímicos/análise , Cromatografia Gasosa-Espectrometria de Massas , Sensibilidade e Especificidade , Espectrofotometria Atômica
13.
J Chromatogr A ; 1117(2): 206-13, 2006 Jun 09.
Artigo em Inglês | MEDLINE | ID: mdl-16600253

RESUMO

The gas chromatographic (GC) speciation of polycyclic aromatic sulfur heterocycles (PASH) in deeply desulfurized fuels is difficult without recourse to authentic standard compounds. Here we investigate the GC retention indexes for 30 alkylated dibenzothiophenes on two methylphenylsiloxane stationary phases of different selectivity (5% and 50% phenyl groups) with a view to avoid the synthesis of all congeners. The influence of the substitution pattern on the retention indexes is discussed. With the measured data it is possible to carry out a multiple linear regression (MLR) to calculate parameters for predicting the retention indexes of unknown polymethylated isomers based only on their structural features. Nine trimethyldibenzothiophene in a synthesis product are identified through their retention indexes. The retention indexes for a total of 43 alkyldibenzothiophenes are listed.


Assuntos
Cromatografia Gasosa/métodos , Tiofenos/classificação , Alquilação , Cromatografia Gasosa-Espectrometria de Massas , Estrutura Molecular , Tiofenos/química
14.
Anal Bioanal Chem ; 373(6): 344-55, 2002 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12172669

RESUMO

The atomic-emission detector in gas chromatography is enormously versatile in applications in analytical chemistry. Its unique properties of high selectivity for most elements and low limits of detection combine to make it the preferred detector for many analytical problems. In this review the stress is laid on the possibility of using it for compound-independent calibration, for determination of the empirical formula of an unknown analyte, for isotope-selective detection, and on derivatization to give AED-active derivatives with advantageous detection properties. Both metals and non-metals are considered and examples of the use of atomic emission detection in real-world analysis are discussed.


Assuntos
Cromatografia Gasosa/métodos , Calibragem , Cloro/análise , Gases/análise , Análise Espectral/métodos , Oligoelementos/análise , Abastecimento de Água/análise
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