More than just sweet: current insights into microplastics in honey products and a case study of Melipona quadrifasciata honey.

Honey, traditionally known as a pure and natural substance, has become an unexpected reservoir for microplastic contamination. This study consisted of an experimental investigation to assess the occurrence of microplastics in honey produced by Melipona quadrifasciata, a native bee species in Brazil. Our investigation covers eight areas (one sample per area), including built and vegetated areas located in São Paulo city, Brazil, to understand the distribution of microplastics in these environments. Honey samples (10 mL) were collected using a syringe and sent to the laboratory for further analysis. Microplastics extracted from honey samples were characterized under a stereomicroscope to determine their size, color, and morphology. Also, the polymer type was determined by FTIR analysis. All honey samples (100%) showed microplastics. The predominant particles displayed a fiber shape with a size below 299 µm and a transparent color and were primarily composed of polypropylene. Their concentrations ranged from 0.1 to 2.6 particles per mL of honey, raising concerns about their potential impact on bee populations and human consumers. This study underscores the need for further research on the sources and implications of microplastic contamination in Melipona quadrifasciata honey, shedding light on the broader issue of environmental plastic pollution and its impact on pollinators.

The retention of plastic particles by macrophytes in the Amazon River, Brazil.

This study evaluated the presence of plastics and microplastics in macrophytes in an urbanized sector of the Amazon River. A total of 77 quadrats in 23 macrophyte banks were sampled during the dry (September 2020) and rainy (June 2021) season. Five species were identified: Paspalum repens, Pontederia rotundifolia, Pistia stratiotes, Salvinia auriculata and Limnobium laevigatum, with P. repens being dominant during the dry season (47.54%) and P. rotundifolia during the rainy season (78.96%). Most of the plastic particles accumulated in Paspalum repens (49.3%) and P. rotundifolia (32.4%), likely due to their morphological structure and volume. The dry season showed a higher accumulation of plastic particles than the rainy season. Microplastics were found in most samples, during both the dry (75.98%) and rainy seasons (74.03%). The upstream macrophyte banks retained more plastic particles compared to the downstream banks. A moderate positive correlation was observed between the presence of plastic particles and macrophyte biomass, and a weak positive correlation between the occurrence of microplastics and mesoplastics. White and blue fragments, ranging from 1 to 5 mm were the most common microplastics found in the macrophyte banks. Green fragments and green and blue fibers were identified as polypropylene, blue and red fragments as polyethylene, and white fragments as polystyrene. Therefore, the results of this study highlight the first evidence of the retention of plastic particles in macrophytes of the Amazon and highlight a significant risk due to the harmful effects that this type of plastic can cause to the fauna and flora of aquatic ecosystems.

Unveiling the hidden threat of microplastics to coral reefs in remote South Atlantic islands.

The widespread presence of marine microplastics (< 5 mm) is a significant concern, as it may harm marine biodiversity and ocean ecosystems. Corals' capacity to ingest microplastics has emerged as a significant threat to reef ecosystems, owing to the detrimental physiological and ecological effects it can trigger. The extent of the impact of microplastics on Brazilian corals remains unclear and this study aimed to investigate its distribution and characteristics in four coral species: Favia gravida, Mussismilia hispida, Montastrea cavernosa, and Siderastrea stellata, found in the Trindade and Martim Vaz Islands - the most isolated archipelago of Brazil, located about 1200 km (680 miles) east of the coast. This study aims to reveal the extent of microplastic distribution in the coral reef environment, assess the amount of microplastics in different coral species, and compare each species' capacity to adhere and accumulate microplastics. A high concentration of ingested and adhered microplastics was detected in all coral species evaluated in the present study. No significant differences were observed in the sampling points which indicates that although the sampling points are located at different distances from the coast, the microplastic pollution is equally distributed in the region. Polyethylene (PE), polyvinyl chloride (PVC), polypropylene (PP), poly(methyl methacrylate) (PMMA), Rayon, and Nylon particles were detected, with a predominance of PE (45.5 %). No significant differences in microplastic concentration were detected among the various species and locations studied. Our research presents findings that demonstrate the extensive occurrence of microplastic contamination in coral colonies located on remote islands.

Microplastic contamination in the freshwater shrimp Macrobrachium amazonicum in Itacoatiara, Amazonas, Brazil.

The present study analyzed the presence of microplastics (MPs) in the shrimp Macrobrachium amazonicum, which is an economically important food that is consumed in several regions of the Brazilian Amazon. A total of 600 specimens of M. amazonicum were captured at two sampling sites (urban and rural area). A total of 2597 MP particles were recorded in the shrimps, with a significant difference between the two sites. The presence of MPs in the body parts also differed significantly. No significant difference was found between MPs abundance and sex of the shrimps. The size of the MPs did not differ significantly between the collection sites and between the body parts. Dark blue fiber-type MPs were the most abundant. A positive correlation was observed between the abundance of MPs and the total weight of shrimps. Raman spectroscopy identified the dark blue fibers as polypropylene and the FTIR technique identified the light blue fragments as nylon. The results indicate that the presence of MPs in the M. amazonicum shrimp is associated with the capture sites near the urban area and is present in the diet of the Amazonian population that regularly consumes this crustacean in traditional dishes.

Biological responses of Chironomus sancticaroli to exposure to naturally aged PP microplastics under realistic concentrations.

Microplastic (MP) is yet another form of chronic anthropogenic contribution to the environment. MPs are plastic particles (<5 mm) that have been widely found in the most diverse natural environments, but their real impacts on ecosystems are still under investigation. Here, we studied the toxicity of naturally aged secondary polypropylene (PP) MPs after constant exposure to ultraviolet radiation (26 µm) to the third instar larvae of Chironomus sancticaroli, a dipteran species. The concentrations tested were 13.5; 67.5; and 135 items g-1 of dry sediment. C. sancticaroli organisms were investigated for fragment ingestion, mortality and changes in enzymatic biomarkers after 144 h of exposure. The organisms were able to ingest MPs from the first 48 h, and the amount of items internalized was dose-dependent and time-dependent. Overall, the results show that mortality was low, being significant at the lowest and highest concentrations (13.5 and 135 items g-1). Regarding changes in biochemical markers, after 144 h MDA and CAT activities were both significantly altered (increased and reduced, respectively), while SOD and GST levels were unchanged. In the present study, naturally aged polypropylene MPs induced biochemical toxicity in C. sancticaroli larvae, with toxicity being higher according to exposure time and particle concentration.

Presence of airborne microplastics in human lung tissue.

Plastics are ubiquitously used by societies, but most of the plastic waste is deposited in landfills and in the natural environment. Their degradation into submillimetre fragments, called microplastics, is a growing concern due to potential adverse effects on the environment and human health. Microplastics are present in the air and may be inhaled by humans, but whether they have deleterious effects on the respiratory system remain unknown. In this study, we determined the presence of microplastics in human lung tissues obtained at autopsies. Polymeric particles (n = 33) and fibres (n = 4) were observed in 13 of 20 tissue samples. All polymeric particles were smaller than 5.5 µm in size, and fibres ranged from 8.12 to 16.8 µm. The most frequently determined polymers were polyethylene and polypropylene. Deleterious health outcomes may be related to the heterogeneous characteristics of these contaminants in the respiratory system following inhalation.

Extra and intracellular synthesis of nickel oxide nanoparticles mediated by dead fungal biomass.

The use of dead biomass of the fungus Hypocrea lixii as a biological system is a new, effective and environmentally friendly bioprocess for the production and uptake of nickel oxide nanoparticles (NPs), which has become a promising field in nanobiotechnology. Dead biomass of the fungus was successfully used to convert nickel ions into nickel oxide NPs in aqueous solution. These NPs accumulated intracellularly and extracellularly on the cell wall surface through biosorption. The average size, morphology and location of the NPs were characterized by transmission electron microscopy, high-resolution transmission electron microscopy, scanning electron microscopy, and energy dispersive X-ray spectroscopy. The NPs were mainly spherical and extra and intracellular NPs had an average size of 3.8 nm and 1.25 nm, respectively. X-ray photoelectron spectroscopy analysis confirmed the formation of nickel oxide NPs. Infrared spectroscopy detected the presence of functional amide groups, which are probable involved in particle binding to the biomass. The production of the NPs by dead biomass was analyzed by determining physicochemical parameters and equilibrium concentrations. The present study opens new perspectives for the biosynthesis of nanomaterials, which could become a potential biosorbent for the removal of toxic metals from polluted sites.

High performance gold nanorods and silver nanocubes in surface-enhanced Raman spectroscopy of pesticides.

The behavior of Au nanorods and Ag nanocubes as analytical sensors was evaluated for three different classes of herbicides. The use of such anisotropic nanoparticles in surface-enhanced Raman scattering (SERS) experiments allows the one to obtain the spectrum of crystal violet dye in the single molecule regime, as well as the pesticides dichlorophenoxyacetic acid (2,4-D), trichlorfon and ametryn. Such metallic substrates show high SERS performance at low analyte concentrations making them adequate for use as analytical sensors. Density functional theory (DFT) calculations of the geometries and vibrational wavenumbers of the adsorbates in the presence of silver or gold atoms were used to elucidate the nature of adsorbate-nanostructure bonding in each case and support the enhancement patterns observed in each SERS spectrum.