RESUMO
Catfish reovirus (CRV), a double stranded RNA virus, inhibited channel catfish herpes-virus (CCV) replication by 2 different mechanisms: (1) directly as a consequence of its own replication, and (2) indirectly due to the induction of an anti-viral factor. In the former, prior infection with CRV significantly reduced subsequent CCV protein synthesis and virus yield. CRV mediated-interference was greatest when CRV infection preceded CCV infection by 16 h, and was least when cell cultures were simultaneously infected with both viruses. in the latter case, the infection of channel catfish ovary (CCO) cultures with UV-inactivated CRV resulted in the synthesis (or release) of an anti-viral factor. Cells producing the factor were protected from CCV infection, as were cells which had been treated with spent culture medium containing anti-viral activity. Interestingly an anti-viral activity was constitutively present in long-term cultures of catfish T-cells and macrophages. Whether this factor and the one induced by UV-inactivated CRV are identical is not known, but analogy to mammalian systems suggests that the former may be similar to type II interferon, whereas the latter may be the piscine equivalent of type I interferon. These results suggest that UV-inactivated CRV may prove useful in the induction and characterization of interferon-like anti-viral proteins in the channel catfish and that long-term cultures of catfish T-cells and monocytes may serve as a ready source of additional anti-viral factors.
Assuntos
Herpesviridae/fisiologia , Ictaluridae/virologia , Interferons/biossíntese , Reoviridae/imunologia , Interferência Viral , Replicação Viral/imunologia , Animais , Linhagem Celular , Feminino , Doenças dos Peixes/imunologia , Doenças dos Peixes/virologia , Herpesviridae/imunologia , Ictaluridae/imunologia , Interferons/imunologia , Tecido Linfoide/citologia , Tecido Linfoide/imunologia , Tecido Linfoide/virologia , Macrófagos/citologia , Macrófagos/imunologia , Macrófagos/virologia , Ovário/citologia , Ovário/imunologia , Ovário/virologia , Reoviridae/fisiologia , Reoviridae/efeitos da radiação , Linfócitos T/citologia , Linfócitos T/imunologia , Linfócitos T/virologia , Raios UltravioletaRESUMO
Two different cDNA sequences for major histocompatibility complex (MHC) class II beta chains from the channel catfish (Ictalurus punctatus) have been identified. Homology between these sequences and those previously identified as MHC class II B genes in other teleosts suggests they represent alleles of the DAB locus. The inferred amino acid sequences show strong evidence for a functional polypeptide chain with a peptide binding region. Southern blot analysis reveals polymorphism in the MHC class II B gene(s) of the channel catfish and suggests the presence of two to four genes.