RESUMO
The nature of SRV-4 infection in cynomolgus macaques remains unclear to date. Here, we report the monitoring of 24 cynomolgus monkeys that were naturally infected with SRV-4 for virus isolation, proviral load and antibody. The results indicated that the SRV-4 antibody status was statistically correlated to environmental temperature.
Assuntos
Anticorpos Antivirais/imunologia , Doenças dos Macacos/imunologia , Doenças dos Macacos/virologia , Infecções por Retroviridae/veterinária , Retrovirus dos Símios/imunologia , Animais , Macaca fascicularis , Dados de Sequência Molecular , Infecções por Retroviridae/imunologia , Infecções por Retroviridae/virologia , Retrovirus dos Símios/genética , Retrovirus dos Símios/isolamento & purificação , Retrovirus dos Símios/fisiologiaRESUMO
At least 5 serotypes of exogenous simian retrovirus type D (SRV/D) have been found in nonhuman primates, but only SRV-1, 2 and 3 have been completely sequenced. SRV-4 was recovered once from cynomolgus macaques in California in 1984, but its genome sequences are unknown. Here we report the second identification of SRV-4 and its complete genome from infected cynomolgus macaques with Indochinese and Indonesian/Indochinese mixed ancestry. Phylogenetic analysis demonstrated that SRV-4 was distantly related to SRV-1, 2, 3, 5, 6 and 7. SRV/D-T, a new SRV/D recovered in 2005 from cynomolgus monkeys at Tsukuba Primate Center in Japan, clustered with the SRV-4 isolates from California and Texas and was shown to be another occurrence of SRV-4 infection. The repeated occurrence of SRV-4 in cynomolgus monkeys in different areas of the world and across 25years suggests that this species is the natural host of SRV-4.
Assuntos
Genoma Viral , Macaca fascicularis/virologia , Doenças dos Macacos/virologia , Infecções por Retroviridae/veterinária , Retrovirus dos Símios/genética , Análise de Sequência de DNA , Infecções Tumorais por Vírus/veterinária , Animais , California , Japão , Dados de Sequência Molecular , Infecções por Retroviridae/virologia , Retrovirus dos Símios/classificação , Retrovirus dos Símios/isolamento & purificação , Texas , Infecções Tumorais por Vírus/virologia , Proteínas Virais/genéticaRESUMO
PURPOSE: The purpose of this study was to evaluate the effect of hyperbaric oxygen therapy on bone regeneration during distraction of irradiated rabbit mandibles. MATERIALS AND METHODS: Twenty New Zealand white rabbits were randomly sub-divided into 4 groups. Group 1 served as control, group 2 received preoperative radiation therapy, group 3 received pre- and postoperative hyperbaric oxygen (HBO) therapy, and group 4 received preoperative radiation therapy and pre- and postoperative HBO therapy. All rabbits underwent a corticotomy of the left body of the mandible after placement of a distraction device. Distraction, at a rate of 1 mm/day and a rhythm of 1 turn/day, began after a 3-day latency period for 14 days. Thirty days after completion of the distraction protocol, the animals were euthanized, and histomorphometric and radiographic data of the distraction segments were obtained. RESULTS: Histomorphometric analysis of new bone fill was greatest in the non-irradiated groups compared to groups receiving radiation therapy, regardless of HBO therapy (P = .03). Pre-corticotomy bone density measurements showed a significant increase in bone density over time (P = .0007). This resulted in a significant relationship between HBO therapy, radiation therapy, and time (P = .0050). CONCLUSIONS: The results of the study support the use of HBO therapy during distraction osteogenesis. Any additional therapeutic benefit of HBO therapy in irradiated bone would require additional investigation.