RESUMO
Polymerase chain reaction was used to determine the prevalence and correlates of human herpesvirus 8 (HHV8) in saliva, mouth, cervical, vaginal, plasma, and peripheral-blood mononuclear cell (PBMC) samples from 174 HHV8-seropositive female prostitutes in Mombasa, Kenya. The prevalence of detection of HHV8 was 32% in saliva samples, 28% in mouth swabs, 4% in cervical swabs, 2.3% in vaginal swabs, 9% in plasma samples, and 18% in PBMC samples. Human immunodeficiency virus type 1 (HIV-1) seropositivity was associated with detection of HHV8 from any mucosal surface (odds ratio, 2.1 [95% confidence interval, 1.1-4.0]). In HIV-1-seropositive women, there was no association between detection of HHV8 and either CD4 count or HIV-1 viral load.
Assuntos
Genitália Feminina/virologia , Soronegatividade para HIV , Soropositividade para HIV/complicações , Herpesvirus Humano 8/isolamento & purificação , Boca/virologia , Eliminação de Partículas Virais , Adolescente , Adulto , Colo do Útero/virologia , Estudos de Coortes , Estudos Transversais , DNA Viral/sangue , Feminino , Genitália Feminina/metabolismo , Herpesvirus Humano 8/genética , Herpesvirus Humano 8/imunologia , Humanos , Quênia/epidemiologia , Leucócitos Mononucleares/virologia , Boca/metabolismo , Prevalência , Estudos Prospectivos , Fatores de Risco , Saliva/virologia , Sarcoma de Kaposi/complicações , Sarcoma de Kaposi/virologia , Trabalho Sexual , Vagina/virologiaRESUMO
Serological tests for herpes simplex virus (HSV) that can accurately distinguish between HSV-1 and HSV-2 are now commercially available. These tests detect antibodies to HSV glycoproteins G-1 and G-2, which evoke a type-specific antibody response. Focus Technologies produces the HerpeSelect-1 and HerpeSelect-2 enzyme-linked immunosorbent assay tests and the HSV-1 and HSV-2 HerpeSelect1/2 Immunoblot. Diagnology has marketed POCkit-HSV-2, a point-of-care test for HSV-2 that allows blood from a finger stick to be tested in a clinic. These tests can be used to confirm a genital herpes diagnosis, establish diagnosis of HSV infection in patients with atypical complaints, identify asymptomatic carriers, and identify persons at risk for acquiring HSV. Potential settings for use of these tests include sexually transmitted disease clinics, prenatal clinics, and clinics that care for patients with human immunodeficiency virus. Patient interest in HSV serological tests appears high.