Effects of zinc supplementation in mitigating the harmful effects of chronic cadmium exposure in a zebrafish model.

Cadmium (Cd) is a heavy metal that is highly toxic to living organisms, including humans. But the dietary zinc (Zn) supplements play critical role in minimizing or preventing Cd poisoning, without any side effects. The underlying mechanisms, however, have not been thoroughly investigated. Therefore, in this study, we investigated the use of Zn as a protection against Cd toxicity in zebrafish models. The obtained results confirmed the levels of antioxidant enzymes and supported the synergistic effects of Zn in reducing Cd toxicity. The lipid, carbohydrate, and protein concentrations in the liver tissue have also been negatively impacted by Cd; however, treatment with Zn has lessened these adverse effects. Furthermore, the level of 8-hydroxy-2' -deoxyguanosine (8-OHdG), caspase-3 also confirms the protective effects of Zn in reducing DNA damage caused by Cd. The results of this study demonstrate that a Zn supplement can lessen the harmful effects of Cd in zebrafish model.

Novel 1, 4-dihydropyridines for L-type calcium channel as antagonists for cadmium toxicity.

The present study, we design and synthesize the novel dihydropyridine derivatives, i.e., 3 (a-e) and 5 (a-e) and evaluated, anticonvulsant activity. Initially due to the lacuna of LCC, we modeled the protein through modeller 9.15v and evaluated through servers. Docking studies were performed with the synthesized compounds and resulted two best compounds, i.e., 5a, 5e showed the best binding energies. The activity of intracellular Ca2+ measurements was performed on two cell lines: A7r5 (rat aortic smooth muscle cells) and SH-SY5Y (human neuroblastoma cells). The 5a and 5e compounds was showing the more specific activity on L-type calcium channels, i.e. A7r5 (IC50 = 0.18 ± 0.02 and 0.25 ± 0.63 µg/ml, respectively) (containing only L-type channels) than SH-SY5Y (i.e. both L-type and T-type channels) (IC50 = 8 ± 0.23 and 10 ± 0.18 µg/ml, respectively) with intracellular calcium mobility similar to amlodipine. Finally, both in silico and in vitro results exploring two derivatives 5a and 5e succeeded to treat cadmium toxicity.