RESUMO
Our studies showed that the spin-labeled (SL) compounds (free stable nitroxyl radical derivatives) accumulate predominantly in pigment melanoma and some of them possess high antimelanoma activity (100% curability of tumor bearing animals) and lower general toxicity. Taking into account our results we describe a proposed mechanism of the spin-labeled accumulation in melanoma, their antimelanoma activity and lower toxicity.
Assuntos
Melanoma/etiologia , Melanoma/metabolismo , Óxidos de Nitrogênio/química , Marcadores de Spin , Antineoplásicos/uso terapêutico , Humanos , Modelos Químicos , Modelos Teóricos , Compostos de Nitrosoureia/química , Pigmentos Biológicos , Silício/química , Triazenos/química , Células Tumorais CultivadasRESUMO
A fraction from crude extract of Galega officinalis L. was purified by gel filtration on Sephadex G-25, Sepharose 4B, and ion-exchange chromatography on diethylaminoethyl (DEAE)-cellulose. The fraction with molecular weight 100-140 kDa appears to have a polysaccharide nature, including protein. The fraction inhibits platelet aggregation initiated by 25 microM adenosine 5'-diphosphate (ADP), 100 microg/ml collagen, and 0.8 U/ml thrombin with the 50% inhibiting concentration (IC(50)) being 11.2 microg/ml for ADP, and the IC(100) being 15.1 microg/ml for collagen and IC(100) 19.6 microg/ml for thrombin.