Phenolic Composition of Crataegus monogyna Jacq. Extract and Its Anti-Inflammatory, Hepatoprotective, and Antileukemia Effects.

Crataegus monogyna (C. monogyna) is a prominent plant used in Moroccan traditional medicine. This study investigated the phenolic composition and the anti-inflammatory, the hepatoprotective, and the anticancer activities of a hydroethanolic extract of C. monogyna leaves and stems. Ultra-high-performance liquid chromatography identified the phenolic profile. The in vitro anticancer activity was evaluated using the MTT assay on HL-60 and K-562 myeloleukemia cells and liver (Huh-7) cell lines. The anti-inflammatory effect was assessed in vivo using carrageenan-induced paw edema in rats. The hepatoprotective effect at 300 and 1000 mg/kg doses against the acetaminophen-induced hepatotoxicity on rats was studied for seven days. Additionally, molecular docking simulations were performed to evaluate the extract's inhibitory potential against key targets: lipoxygenase, cytochrome P450, tyrosine kinase, and TRADD. The extract exhibited significant cytotoxic activity against K-562 and HL-60 cells, but not against lung cancer cells (Huh-7 line). The 1000 mg/kg dose demonstrated the most potent anti-inflammatory effect, inhibiting edema by 99.10% after 6 h. C. monogyna extract displayed promising hepatoprotective properties. Procyanidin (-7.27 kcal/mol), quercetin (-8.102 kcal/mol), and catechin (-9.037 kcal/mol) were identified as the most active molecules against lipoxygenase, cytochrome P450, and tyrosine kinase, respectively. These findings highlight the untapped potential of C. monogyna for further exploration in treating liver damage, inflammation, and leukemia.

Immunomodulatory and Anticancer Effects of Fridericia chica Extract-Loaded Nanocapsules in Myeloid Leukemia.

Nanocapsules provide selective delivery and increase the bioavailability of bioactive compounds. In this study, we examined the anticancer and immunomodulatory potential of Fridericia chica (crajiru) extract encapsulated in nanocapsules targeting myeloid leukemias. Nanocapsules containing crajiru (nanocapsules-CRJ) were prepared via interfacial polymer deposition and solvent displacement. Size and polydispersity were measured by dynamic light scattering. Biological assays were performed on leukemia cell lines HL60 and K562 and on non-cancerous Vero cells and human PBMC. The anticancer activity was evaluated using cytotoxicity and clonogenic assays, while the immunomodulatory activity was evaluated by measuring the levels of pro- and anti-inflammatory cytokines in PBMC supernatants treated with concentrations of nanocapsules-CRJ. Nanocapsules-CRJ exhibited significant cytotoxic activity against HL60 and K562 cells at concentrations ranging from 0.75 to 50 µg/mL, with the greatest reductions in cell viability observed at 50 µg/mL (p < 0.001 for HL60; p < 0.01 for K562), while not affecting non-cancerous Vero cells and human PBMCs. At concentrations of 25 µg/mL and 50 µg/mL, nanocapsules-CRJ reduced the formation of HL60 and K562 colonies by more than 90% (p < 0.0001). Additionally, at a concentration of 12 µg/mL, nanocapsules-CRJ induced the production of the cytokines IL-6 (p = 0.0002), IL-10 (p = 0.0005), IL-12 (p = 0.001), and TNF-α (p = 0.005), indicating their immunomodulatory potential. These findings suggest that nanocapsules-CRJ hold promise as a potential therapeutic agent with both cytotoxic and immunomodulatory properties.

Adaptación lingüística y cultural al español chileno del cuestionario de autovaloración vocal para cantantes Singing Voice Handicap Index (S-VHI)

Objetivo: Adaptar lingüística y culturalmente el cuestionario Singing Voice Handicap Index (SVHI) al español chileno. Metodología: Se realizó la traducción directa al español chileno del SVHI y posteriormente la retrotraducción al idioma original inglés; ambas traducciones fueron revisadas por un juicio de expertos bilingües, quienes definieron qué ítems conformarían la primera versión de la herramienta traducida. Esta versión fue respondida por 15 cantantes chilenos con diagnóstico de disfonía, con el objetivo de identificar discrepancias, las que fueron revisadas y consensuadas por el comité de expertos; una segunda versión modificada fue aplicada a 5 cantantes más con diagnóstico de disfonía, no encontrándose en esta fase preguntas incomprendidas o inapropiadas para la cultura chilena. Resultados: Se modificaron 5 de los 9 ítems cuestionados para llegar la versión final que refleja la versión original del inglés, tanto en la cantidad de ítems como en los dominios de la encuesta. Limitaciones: Por la cantidad de personas participantes, se pudo realizar solamente la adaptación cultural y no la validación del instrumento que considere puntajes de corte para la población estudiada. Por lo tanto, la herramienta puede ser usada para realizar un análisis cualitativo y no cuantitativo. Valor: Contar con esta encuesta traducida, siguiendo un proceso metodológico riguroso, permite que cantantes chilenos manifiesten su desventaja vocal relacionada con el canto, además de identificar los dominios más afectados y, de esa forma, planificar de manera más específica la intervención vocal. Conclusión: Se cuenta con el cuestionario Índice de desventaja en voz cantada para el español chileno para su aplicación al idioma y cultura chilena.

Purpose: To adapt the Singing Voice Handicap Index (SVHI) questionnaire linguistically and culturally to Chilean Spanish. Methodology: The SVHI was directly translated into Chilean Spanish and later back-translated into the original English language; both translations were reviewed by a judgment of bilingual experts, who defined which items would make up the first version of the translated tool. This version was answered by 15 Chilean singers with a diagnosis of dysphonia, with the aim of identifying discrepancies, which were reviewed and agreed upon by the committee of experts; a second modified version was applied to 5 more singers with a diagnosis of dysphonia, not finding misunderstood or inappropriate questions for Chilean culture at this stage. Results: 5 of the 9 items questioned were modified to reach the final version that reflects the original English version, both in the number of items and in the domains of the survey. Limitations: Due to the number of participating people, only the cultural adaptation could be carried out and not the validation of the instrument that considers cut-off scores for the population studied. Therefore, the tool can be used to carry out a qualitative and not quantitative analysis. Value: Having this survey translated, following a rigorous methodological process, allows Chilean singers to express their singing-related vocal disadvantage and identify the most affected domains and, thus, plan the vocal intervention more specifically. Conclusion: The Singing Voice Disadvantage Index questionnaire for Chilean Spanish is available for its application to the Chilean language and culture.

Pharmacological assessment of the antineoplastic and immunomodulatory properties of a new spiroindolone derivative (7',8'-Dimethoxy-1',3'-dimethyl-1,2,3',4'-tetrahydrospiro[indole-3,5'-pyrazolo[3,4-c]isoquinolin]-2-one) in chronic myeloid leukemia.

The discovery and development of effective novel compounds is paramount in oncology for improving cancer therapy. In this study, we developed a new derivative of spiroindolone (7',8'-Dimethoxy-1',3'-dimethyl-1,2,3',4'-tetrahydrospiro[indole-3,5'- pyrazolo[3,4-c]isoquinolin]-2-one) and evaluated its anticancer- and immunomodulatory potential in a vitro model of chronic leukemia. We utilized the chronic leukemia cell line K562, as well as non-cancerous peripheral blood mononuclear cells (PBMC) and Vero cells (kidney epithelium of Cercopithecus aethiops). We assessed the cytotoxicity of the compound using the MTT assay, and performed cell cycle assays to determine its impact on different stages of the cell cycle. To evaluate its antineoplastic activity, we conducted a colony formation test to measure the effect of the compound on the clonal growth of cancer cells. Furthermore, we evaluated the immunomodulatory activity of the compound by measuring the levels of pro and anti-inflammatory cytokines. The study findings demonstrate that the spiroindolone-derived compound exerted noteworthy cytotoxic effects against K562 cells, with an IC50 value of 25.27 µg/mL. Additionally, it was observed that the compound inhibited the clonal proliferation of K562 cells while displaying minimal toxicity to normal cells. The compound exhibited its antiproliferative activity by inducing G2/M cell cycle arrest, preventing the entry of K562 cells into mitosis. Notably, the compound demonstrated an immunomodulatory effect by upregulating the production of cytokines IL-6 and IL-12/23p40. In conclusion, the spiroindolone-derived compound evaluated in this study has demonstrated significant potential as a therapeutic agent for the treatment of chronic myeloid leukemia. Further investigations are warranted to explore its clinical applications.

Phenolic Composition, Wound Healing, Antinociceptive, and Anticancer Effects of Caralluma europaea Extracts.

Caralluma europaea (Guss.) is an important medicinal plant widely used in Morocco for various traditional purposes. Our work aimed to evaluate the phenolic composition, wound healing, antinociceptive, and anticancer activities of C. europaea extracts. Moreover, this study assessed the beneficial effect of C. europaea phytocompounds on the TRADD, cyclooxegenase-2, Wnt/ß-catenin, and tyrosine kinase signaling pathways. The wound healing effect of C. europaea formulations against skin burn was evaluated for 21 days. The cytotoxic effect of the C. europaea extracts was evaluated against human leukemic (K562 and HL60) and liver cancer cell lines (Huh-7) using the MTT test. All the phytoconstituents identified by UHPLC in the polyphenols were docked for their inhibitory power on protein casein kinase-1, glycogen synthase kinase-3-ß, cyclooxegenase-2, tyrosine kinase, and TRADD. Luteolin and kaempferol are the main compounds identified in C. europaea polyphenols. The group treated with polyphenols showed the greatest wound contractions and all tested extracts presented a significant antinociceptive effect. Polyphenols showed a remarkable antitumoral activity against the K562, HL60 and Huh-7 cell lines. Saponins exerted an important cytotoxic effect against the Huh-7 cell line, whereas no cytotoxicity was observed for the hydroethanolic and flavonoids extracts. Hesperetin and trimethoxyflavone presented the highest docking G-score on tyrosine kinase and cyclooxygenase, respectively.

Perfil sociodemográfico de pessoas trans acompanhadas pelo serviço de atenção especializada e transdisciplinar da Universidade Federal de São Paulo / Socio-demographic profile of trans persons followed by the specialized and transdisciplinary care service of the Federal University of Sao Paulo

Introdução: O ambulatório do Núcleo de Estudos, Pesquisa, Extensão e Assistência à Pessoa trans Professor Roberto Farina da Universidade Federal de São Paulo - Núcleo TransUnifesp (NTU) - desenvolve um conjunto de atividades transdisciplinares sobre transgeneridade e intersexualidade. Sua equipe é composta por profissionais de diferentes áreas que possuem como diretriz o cuidado do sujeito em sua integralidade. Objetivos: Descrever, na forma de relato de experiência, o perfil sociodemográfico das pessoas atendidas. Métodos: Estudo transversal exploratório sobre as características sociodemográficas das pessoas atendidas no ambulatório do NTU entre 2017 e 2022 (5 anos). As informações foram obtidas por autodeclaração a partir do preenchimento do cadastro por ocasião do acolhimento. Resultados: Dos 255 cadastros, verificamos que a idade das pessoas variou de 18 aos 73 anos (média 29,8 anos). Dessas, 109 (42,5%) declararam terem feito a retificação de seus documentos. Quanto à raça/cor, 143 (56,0%) se declararam brancas, 66 (25,8%) pardas, 29 (11,3%) pretas, 4 (1,5%) amarelas, 3 (1,1%) indígenas e de 10 (3,9%) não obtivemos informação. Do total, 191 (74,9%) referiram ser casadas, 48 (18,9%) solteiras e 16 (6,2%) não informaram. Em relação ao gênero, 122 (47,8%) são mulheres, 87 (34,1%) homens trans, 22 (8,6%) travestis, 10 (3,9%) não binárias, 3 (1,2%) sem gênero; 2 (0,8%) queer; 1 (0,4%) cross-dresser, 4 (1,5%) outra e 4 (1,5%) não informaram. Quanto à escolaridade, 21 (8,2%) possuem o fundamental incompleto, (2,3%) fundamental completo, 19 (7,4%) médio incompleto, 87 (34,1%) médio completo; 70 (27,4%) superior incompleto, 37 (14,5%) superior completo, 8 (3,1%) com pós-graduação e 7 (2,7%) não informaram. Quanto a procedência, 105 (41,2%) moram na capital (São Paulo) e 143 (56,1%) fora. Em relação à orientação sexual, 137 (53,7%) se declararam heterossexuais, 22 (8,6%) bissexuais, 17 (6,7%) homossexuais, 7 (2,7%) assexuais, 4 (1,6%) pansexuais, 6 (2,4%) com outras orientações e 62 (24,3%) não informaram. Do total, 41 (16%) eram estudantes, 38 (14,9%) trabalhadores(as) de serviços, 30 (11,7%) do comércio, 19 (7,4%) desempregadas, 17 (6,6%) trabalham como técnicas de nível médio, 16 (6,2%) na indústria, 12 (4,7%) em serviço administrativo, 12 (4,7%) e 11 (4,3%) como profissionais das ciências sociais ou humanas e ciências biológicas ou da saúde respectivamente, 10 (3,9%) educadoras, 7 (2,7%) do lar; 4 (1,5%) trabalhadoras da lavoura, 23 (9%) em outras ocupações e 15 (5,9%) não informaram. Três pessoas declararam serem trabalhadoras do sexo. Quanto aos procedimentos realizados previamente, 139 (54,5%) fizeram hormonização sem orientação de profissional de saúde, das quais 83 (59,7%) eram mulheres trans, 34 (24,4%) homens trans, 14 (10,0%) travestis, 4 (2,8%) pessoas de gênero não binário e outras 4 com outra identidade. Do total, 25 pessoas (9,8%) relataram a aplicação de silicone industrial. Quanto às demandas em saúde, a busca na clínica pela endocrinologia e saúde mental (psicologia e psiquiatria), e a mastectomia e neovulvovaginoplastia na cirurgia, foram as mais procuradas. Conclusões: A experiência de equipe do NTU reconhece a importância da atenção primária transdisciplinar como porta de entrada para o atendimento de pessoas trans, e da atenção especializada ofertada em rede, especialmente quando ligado à universidade, na produção de conhecimento e formação de profissionais, garantindo maior suporte em situações de demandas complexas, contribuindo com a ampliação do atendimento integral e com o acesso aos equipamentos de saúde específicos.

Adaptación lingüística y cultural chilena del Voice Handicap Index (VHI) y del Voice Handicap Index 10 (VHI-10) / Chilean linguistic and cross-cultural adaptation of the Voice Handicap Index (VHI) and the Voice Handicap Index 10 (VHI-10)

El presente estudio buscó adaptar lingüística y culturalmente el cuestionario de autovaloración vocal norteamericano Voice Handicap Index (VHI) al español chileno. Para esto se realizaron cuatro traducciones directas del VHI al español chileno de forma independiente. Luego, se consensuó una traducción general (TG) a partir del juicio de tres profesionales de la fonoaudiología. Esta TG fue traducida inversamente al idioma original para ser comparada con la versión original por el mismo grupo de profesionales de la fonoaudiología, llegando a la primera versión del Índice de Desventaja Vocal Chileno(IDV-Cl). Esta versión fue aplicada a veinte personas con diagnóstico de disfonía, considerando una escala Likert de frecuencia. Durante la adaptación, se consideró pertinente dejar constancia de las consignas no comprendidas o que resultaron inapropiadas culturalmente para el vocabulario chileno. De acuerdo con las observaciones recogidas, se revisó y modificó el instrumento, dando paso a la segunda versión del IDV-Cl, que fue aplicada a seis personas con diagnóstico de disfonía. En esta etapa no se plantearon nuevas sugerencias de cambio. El IDV-Cl refleja la versión original del inglés, tanto en la cantidad de ítems, como en los subcomponentes físico, funcional y emocional que lo conforman. Mediante la síntesis de esta encuesta se pudo obtener también una versión abreviada, que consta de diez ítems (IDV-Cl-10). En conclusión, se establece la equivalencia cultural y lingüística del cuestionario VHI en español chileno, del que se origina la versión IDV-Cl e IDV-Cl-10.

The present study aimed to linguistically and culturally adapt the North American vocal self-assessment questionnaire, Voice Handicap Index (VHI), to Chilean Spanish. To this end, four direct translations of the VHI were carried out independently into Chilean Spanish. Subsequently, a general translation (GT) was agreed upon based on the judgment of three speech-language therapists. This GT was back-translated into the original language and compared with the original version by the same group of speech therapy professionals, which resulted in the first version of the Chilean Índice de Desventaja Vocal [Vocal Disadvantage Index] (IDV-Cl). This version was then applied to twenty people diagnosed with dysphonia, using a Likert scale for frequency. During the adaptation, it was deemed pertinent to make a note of the statements that were not understood or were culturally inappropriate for the Chilean vocabulary. The instrument was revised and modified based on the observations made by the participants, thus creating a second version of the IDV-Cl, which was then applied to six people diagnosed with dysphonia. No new suggestions for change were made at this stage. The IDV-Cl reflects the original version in English, both in the number of items and in the physical, functional, and emotional subcomponents that comprise it. Through the synthesis of this survey, it was also possible to obtain an abbreviated version, consisting of ten items (IDV-Cl-10). In conclusion, the cultural and linguistic equivalence of the VHI questionnaire in Chilean Spanish is established, resulting in the IDV-Cl and IDV-Cl-10 versions of this instrument.

Prevalência de comportamento suicida em estudantes de Medicina / Prevalence of suicidal behavior in Medical students

Resumo: Introdução: Médicos e alunos de Medicina são grupos de risco para o suicídio e comportamento suicida. Comportamentos suicidas abrangem fenômenos que vão desde pensamentos, planejamentos, tentativas e até a morte por suicídio. Sabe-se pouco sobre o comportamento suicida entre estudantes de Medicina brasileiros. Objetivo: Este estudo teve como objetivos avaliar a prevalência de ideação, planejamentos e tentativas suicidas em uma amostra de estudantes de graduação em Medicina do Brasil, e identificar os fatores sociodemográficos, de vida estudantil e de saúde mais associados a esses comportamentos. Método: Participaram do estudo 722 alunos do curso de Medicina da Unicamp, durante os anos de 2017 e 2018, que responderam de forma voluntária e anônima a um questionário amplo, que incluía dados sociodemográficos, de vida acadêmica e de comportamento suicida. A análise estatística foi realizada por meio do teste de qui-quadrado, do teste de Mann-Whitney e da regressão logística múltipla. Adotou-se o nível de significância estatística de 95%. Resultado: As prevalências de pensamentos, planejamento e tentativas de suicídio ao longa da vida foram respectivamente 196 (27,3%), 64 (8,9%) e 26 (3,6%). Nos 30 dias que antecederam a pesquisa, 36 (5%) pensaram seriamente em pôr fim à própria vida, e 11 (1,5%) planejaram concretamente colocar fim a própria vida. Bullying, presença de transtorno mental, procura de assistência em saúde mental na universidade, uso de calmante sem prescrição médica, baixo nível socioeconômico, morar sozinho, religião (ateus, agnósticos e espiritualistas) e grau de religiosidade são os fatores que, conjuntamente, melhor explicam a chance de comportamento suicida. Conclusão: Alunos de Medicina apresentam prevalências importantes de comportamento suicida.

Abstract: Introduction: Physicians and medical students constitute groups at risk for suicide and suicidal behavior. Suicidal behaviors encompass phenomena ranging from thoughts, planning, and finally death by suicide. Little is known about suicidal behavior among Brazilian medical students. Objective: The aim of this study was to assess the prevalence of suicidal ideation, planning and suicide attempt in a sample of undergraduate medical students in Brazil, as well as to identify the sociodemographic, student life aspects and health factors most often associated with suicidal behavior. Method: A total of 722 medical students at Unicamp, during 2017 and 2018, voluntarily and anonymously answered a broad questionnaire, including sociodemographic data, aspects of academic life and suicidal behavior. A statistical analysis was performed using the chi-square test, Mann-Whitney test, and multivariate logistic regression. A statistical significance level of 95% was adopted. Results: The lifetime prevalence rates of suicidal thoughts, planning and attempts were respectively 196 (27.3%), 64 (8.9%), and 26 (3.6%). In the 30 days prior to the survey, 36 (5%) seriously thought about ending their own lives, and 11 (1.5%) concretely planned to end their own lives. Bullying, presence of mental disorder, seeking mental health care at the university, use of sedatives without a prescription, low socioeconomic level, living alone, religion (atheists, agnostics and spiritualists) and degree of religiousness are the factors that, together, best explain the chance of suicidal behavior. Conclusion: Medical students show important prevalence rates of suicidal behavior.

Adolescent Female Victims of Sexual Violence: Analysis of Loss of Follow-up after Emergency Care and Outpatient Follow-up / Meninas adolescentes vítimas de violência sexual: Análise da falta de atendimento de emergência e seguimento ambulatorial

Abstract Objective To assess the loss to follow-up after emergency care and during 6-months of outpatient follow-up, and the associated variables, among adolescent sexual violence survivors. Methods This is a retrospective study with review of the medical records of 521 females, aged 10 to 18 years, who received emergency care in a referral service in São Paulo, Brazil. The variables were sociodemographic; personal history; characteristics of abuse, disclosure, and reactions triggered after abuse (physical and mental disorders as well as social changes), psychotropic prescription needs, and moment of abandonment: after emergency care and before completing 6 months of outpatient follow-up. To compare groups of patients lost to follow-up at each time point, we used the Chi-square and Fisher exact tests followed by multiple logistic regression with stepwise criterion for selection of associated variables. We calculated the odds ratio with confidence interval (OR, CI 95%). The level of significance adopted was 5%. Results A total of 249/521 (47.7%) adolescents discontinued follow-up, 184 (35.3%) after emergency care and 65 (12.4%) before completing outpatient follow-up. The variables of living with a partner (OR = 5.94 [CI 95%; 2.49-14.20]); not having a religion (OR = 2.38 [CI 95%;1.29-4.38)]), having a Catholic religion [OR = 2.11 (CI 95%; 1.17-3.78)]; and not disclosing the abuse [OR = 2.07 (CI 95%; 1.25-3.44)] were associated with loss to follow-up after emergency care. Not needing mental disorder care (OR = 2.72 [CI 95%; 1.36-5.46]) or social support (OR = 2.33 [CI 95%; 1.09-4.99]) were directly associated with loss to outpatient follow-up. Conclusion Measures to improve adherence to follow-up should be aimed at adolescents who live with a partner and those who do not tell anyone about the violence.

Resumo Objetivos Avaliar a perda de seguimento de adolescentes vítimas de violência sexual após o atendimento de emergência, durante o seguimento ambulatorial e as variáveis associadas. Métodos Estudo retrospectivo com a revisão de prontuários de 521 mulheres de 10 a 18 anos, que buscaram atendimento de emergência em um serviço de referência em São Paulo, Brasil. As variáveis foram sociodemográficas; antecedentes pessoais; características do abuso, atitude de revelação e reações desencadeadas após o abuso (distúrbios físicos, mentais e mudanças sociais), necessidades de prescrição de psicotrópicos e momento do abandono: após atendimento de emergência e antes de completar 6 meses de seguimento ambulatorial. Para comparar os grupos de perda de seguimento em cada momento, foram utilizados os testes do qui-quadrado e exato de Fisher, seguidos de regressão logística múltipla com critério stepwise para seleção das variáveis associadas. Calculamos a razão de probabilidade com intervalo de confiança (RP, IC 95%). O nível de significância adotado foi de 5%. Resultados Um total de 249 (47,7%) das adolescentes descontinuaram o acompanhamento, 184 (35.3%) após o atendimento de emergência e 65 (12.4%) antes de completar o seguimento ambulatorial. As variáveis de viver com companheiro [RP = 5,94 (IC 95%; 2,49-14,20]; não ter religião [RP = 2,38 (IC 95%;1,29-4,38)], ter religião católica [RP = 2,11 (IC 95%; 1,17-3,78)] e não revelar o abuso [RP = 2,07 (IC 95%; 1,25-3,44)] foram associadas à perda de seguimento após o atendimento de emergência. Não necessitar de cuidados de saúde mental (RP = 2,72 [IC 95%; 1,36-5,46]) ou apoio social (RP = 2,33 [IC 95%; 1,09-4,99]) foram as variáveis associadas à perda do seguimento ambulatorial. Conclusão Medidas para melhorar a adesão ao seguimento devem ser direcionadas às adolescentes que vivem com parceiro e às que não revelam a violência sofrida.

Estimativa da idade aplicada a investigações arqueológicas utilizando espectroscopia RPE ­ uma revisão / Dental age estimation applied to archaeological investigations using spectroscopy ESR ­ a review

A estimativa da idade utilizando dentes humanos é realizada por várias ciências. Enquanto na odontologia e antropologia forense o significado desta estimativa está associado à idade cronológica em indivíduos vivos ou mortos, na arqueologia o significado é outro, e reflete o tempo decorrido desde a formação do dente até a sua descoberta no sítio arqueológico, a chamada datação arqueológica. Diante do potencial uso da datação para identificação de corpos antigos (fossilizados) na área forense, o objetivo deste trabalho é abordar a estimativa da idade de dentes sob a perspectiva da arqueologia, por meio do principal método de datação utilizado: o da espectroscopia por ressonância paramagnética eletrônica (RPE). Com o intuito de verificar as principais aplicações deste método na datação de dentes humanos, foi realizada uma revisão da literatura. Os 18 artigos selecionados mostram que a espectroscopia RPE encontra-se consolidada como método de datação para dentes humanos e tem sido aplicada com sucesso nas últimas décadas, na solução de problemas de diversas pesquisas arqueológicas, ajudando a elucidar questionamentos acerca da dinâmica de ocupação, interações interespecíficas e extinção de várias espécies humanas que habitaram a Terra nas últimas centenas de milhares de anos

Age estimation using human teeth is performed by several fields of knowledge. While in forensic dentistry and anthropology the meaning of this estimate is associated with chronological age in living or dead individuals, in archaeology, the meaning is different and reflects the time elapsed from the formation of the tooth until its discovery at the archaeological site, the so-called archaeological dating. Faced with the potential use of archaeological dating to identify ancient (fossilized) bodies in the forensic area, the aim of this work is to approach the age estimation of teeth from the perspective of archaeology, through the main dating method used: Electron spin resonance (ESR) spectroscopy. In order to verify the main applications of this method for the dating of human teeth, a literature review was carried out. 18 articles were selected and show that ESR spectroscopy is consolidated as a dating method for human teeth and it has been applied, successfully over the last few decades, in the solution of problems of several archaeological researches, helping to clarify questions about the dynamics of occupation, interspecific interactions and extinction of several human species that have inhabited the Earth in the last few hundred thousand years

Sexual Violence Suffered by Women in Early and Late Adolescence: Care Provided and Follow-Up / Violência sexual sofrida por mulheres na adolescência precoce e tardia: Cuidado dispensado e seguimento

Abstract Objective To compare the sexual violence suffered by women in early and late adolescence, the reactions triggered after the aggression, and the care provided. Methods A retrospective study in which we reviewed the medical records of 521 female adolescents treated by a multidisciplinary team at a reference hospital in the city of Campinas, state of São Paulo, Brazil. We analyzed sociodemographic variables, and those pertainin to the characteristics of the episodes of violence, the emergency care, and the physical and psychological reactions observed during the follow-up. For the analysis, the sample was divided into groups of early (10 to 14 years) and late (15 to 18 years) adolescence. We used the Chi-squared/Fisher Exact, Mann-Whitney, and Kruskal-Wallis tests to compare the groups; the level of significance adopted was 5%. Results The early group (n= 242) contained more adolescents who were enrolled in school (p< 0.001), suffered more daytime aggressions (p= 0.031), in their residences (p< 0.001), by an aggressor with whom they were acquainted (p< 0.001), had greater need of legal protection (p= 0.001), and took longer to seek care (p= 0.048). Feelings of guilt, shame, and the perception of violence were similar between the groups. In the late group (n= 279), there was greater consumption of alcohol during the aggression (p= 0,005); they received significantly more prophylaxis treatments; reported more physical symptoms (p= 0.033), sleep disorders (p= 0.003), symptoms of anxiety (p= 0.045), and feelings of anguish (p= 0.011); and had more prescriptions of psychotropics (p= 0.005). Only 52% completed the 6-month follow-up, with no differences between the groups. Conclusion The age groups showed differences in the characteristics of the episodes of violence; early adolescents took longer to seek help, and the late group presented more intense symptoms and psychological worsening during the follow-up. Measures of prevention and specific care aimed at this population are needed.

Resumo Objetivo Comparar a violência sexual sofrida por vítimas no início e no final da adolescência, as reações desencadeadas após a agressão, e o cuidado de saúde dispensado. Métodos Estudo retrospectivo, em que foram revisados os prontuários de 521 mulheres adolescentes atendidas por equipe multiprofissional em hospital de referência em Campinas, São Paulo, Brasil. As variáveis foram sociodemográficas, e aquelas relativas às características da violência, ao atendimento de emergência, e às reações físicas e psicológicas observadas durante o seguimento nos grupos de adolescentes de idade precoce (10a 14 anos) e tardia (15 a 18 anos). Utilizamos os testes do Qui-quadrado/Exato de Fisher, Mann-Whitney e Kruskal-Wallis para comparar os grupos; adotamos o nível de significância de 5%. Resultados O grupo precoce (n= 242) continha maior número de estudantes (p< 0,001), que sofreram mais agressões diurnas (p= 0,031), em suas residências (p< 0,001), por agressor conhecido (p< 0,001), tiveram maior necessidade de proteção legal (p= 0,001), e demoraram mais a procurar atendimento (p= 0,048). Sentimentos de culpa, vergonha e a percepção da violência foram similares entre os grupos. No grupo tardio (n= 279) houve maior consumo de álcool durante a agressão (p= 0,005); as adolescentes receberam significativamente mais tratamentos de profilaxia; relataram mais sintomas físicos (p= 0,033), distúrbios do sono (p= 0,003), sintomas de ansiedade (p= 0,045), e sentimentos de angústia (p= 0,011); e receberam mais prescrições de psicotrópicos (p= 0,005). Apenas 52% completaram o seguimento de 6 meses, sem diferenças entre os grupos. Conclusão Os grupos apresentaram diferenças nas características da violência; as adolescentes precoces chegaram mais tardiamente ao serviço, e o grupo tardio apresentou maior sintomatologia e piora psicológica no seguimento. São necessárias medidas de prevenção e cuidados específicos voltados a essa população.

Resistant Starch Type-2 Supplementation Does Not Decrease Trimethylamine N-Oxide (TMAO) Plasma Level in Hemodialysis Patients.

Dysbiosis is recognized as a new cardiovascular disease (CVD) risk factor in hemodialysis (HD) patients because it is linked to increased generation in the gut of uremic toxins such as trimethylamine N-Oxide (TMAO) from dietary precursors (choline, betaine, or L-carnitine). Nutritional strategies have been proposed to modulate the gut microbiota and reduce the production of these toxins. This study aimed to evaluate the effect of amylose-resistant starch (RS) supplementation on TMAO plasma levels in HD patients.We conducted a randomized, double-blind, placebo-controlled trial (NCT02706808) with patients undergoing HD enrolled in a previous pilot study. The participants were allocated to RS or placebo groups to receive 16 g/d of RS or placebo for 4 weeks. Plasma TMAO, choline, and betaine levels were measured with LC-MS/MS. Fecal microbiome composition was evaluated by 16S ribosomal RNA sequencing, followed by a search for TMA-associated taxa. Anthropometric, routine biochemical parameters, and food intake were evaluated.Twenty-five participants finished the study, 13 in the RS group, and 12 in the placebo group. RS supplementation did not reduce TMAO plasma levels. Moreover, no significant alterations were observed in choline, betaine, anthropometric, biochemical parameters, or food intake in both groups. Likewise, RS was not found to exert any influence on the proportion of potential TMA-producing bacterial taxa in fecal matter.RS supplementation did not influence plasma TMAO, choline, betaine, or fecal taxa potentially linked to TMAO. Thus, RS does not seem to modify the TMA-associated bacterial taxa, precursors of TMAO.Supplemental data for this article is available online at https://doi.org/10.1080/07315724.2021.1967814 .

The Effects in the Upper Airway of Heat and Exposure to Combustion Byproducts of Burning Materials on Larynx of Firefighters.

INTRODUCTION: High exposure to heat and combustion byproducts is associated with health problems and an increased risk of cancer. OBJECTIVE: To study the effects of heat and smoke derived from burning on the upper airway of firefighters. METHODS: Thirteen firefighters underwent a 4-week training program in Compartment Fire Behavior Training simulators while working with live fire, heat, and smoke from pallets and oriented-strand board sheet burns. The firefighters were evaluated before exposure and at the end of the first and fourth weeks of training. The larynx was assessed by the Voice Handicap Index GRBASI scale, laryngoscopy, and peak expiratory flow. RESULTS: There was no change in Voice Handicap Index and GRBASI (only one in three speech therapists judged tension as worse by one point). Laryngoscopy showed alterations in one-third of the firefighters, with the most frequent being increased viscous mucus. Peak expiratory flow decreased significantly from 685 to 552 and 550 L/min in the first and fourth weeks, respectively. CONCLUSIONS: Although participants were protected from gases and smoke during the exercises, which proved to be very effective, the airway was slightly impacted by heat and combustion-derived byproducts.

We need to talk about the use of methylphenidate by medical students - review of the literature / Precisamos falar sobre uso de Metilfenidato por estudantes de medicina - revisão da literatura

Abstract: Introduction: Studies methylphenidate (MPH) has been used by medical students to increase their mental activity and improve the performance required during undergraduate school, generating concern regarding the risks to their physical and mental health. This scenario indicates the need for specifically aimed measures in medical schools. Objective: To review the literature about the use of MPH without medical indication amongst medical students. Method: A thorough review of the literature published in English, Spanish, and Portuguese, between 2013 and 2019, based on data made available by Pubmed and Scielo, utilizing keywords in the three above languages, along the four stages of the selection process. Results and Discussion: Altogether, 224 articles were found, of which 25 were selected after reading, dealing with the use of MPH or 'cognition enhancer' by undergraduate medical students without a doctor's prescription. The research indicated significant variability in the frequency of consumption, related to the investigated pattern of use, use with or without indication, before or after entering University and country where the study was carried out. The most frequent justification for the use without medical indication was to attain improvement in academic performance. A lack of research with a fair appraisal of the cognition, behavioral and psychic risks involved, among them addiction and the approach of the topic in medical schools, was noted. Conclusion: The high rates of usage of MPH by medical students aiming at cognitive enhancement strengthens the importance of preventative actions in medical schools. The strategies must consider information concerning the risks of use (of MPH) without medical indication; non-pharmacological interventions for performance improvement; sleep hygiene measures organization for adequate study activities; broad discussions about ethical aspects and curricular structure.

Resumo: Introdução: Estudos mostram que o metilfenidato (MPH) tem sido utilizado por estudantes de medicina para aumentar sua atividade mental e melhorar o desempenho exigido durante a graduação, gerando preocupações quanto aos riscos à sua saúde física e mental. Esse cenário indica a necessidade de medidas especificamente direcionadas nas escolas médicas. Objetivo: Revisar a literatura sobre o uso de MPH sem indicação médica entre estudantes de medicina. Método: Revisão minuciosa da literatura publicada em inglês, espanhol e português, entre 2013 e 2019, com base em dados disponibilizados pelo PUBMED e SCIELO, utilizando palavras-chave nos três idiomas acima, ao longo das quatro etapas do processo de seleção. Resultados e Discussão: Ao todo, foram encontrados 224 artigos, dos quais 25 foram selecionados após leitura, tratando do uso de MPH ou 'potencializador da cognição' por graduandos de medicina sem prescrição médica. A pesquisa indicou variabilidade significativa na frequência de consumo, relacionada ao padrão de uso investigado, uso com ou sem indicação, antes ou após a entrada na Universidade e país onde o estudo foi realizado. A justificativa mais frequente para o uso sem indicação médica foi a de obter melhora no desempenho acadêmico. Notou-se a carência de pesquisas com uma avaliação adequada dos riscos cognitivos, comportamentais e psíquicos envolvidos, entre eles o risco de adição e a abordagem do tópico nas escolas médicas. Conclusão: As altas taxas de uso do MPH por estudantes de medicina visando o aprimoramento cognitivo reforça a importância de ações preventivas nas escolas médicas. As estratégias devem considerar informações sobre os riscos do uso (do MPH) sem indicação médica; intervenções não farmacológicas para melhoria do desempenho cognitivo; medidas de higiene do sono; organização para atividades de estudo adequadas; amplas discussões sobre aspectos éticos e estrutura curricular.

Association between health behaviors and depression: findings from the 2019 Brazilian National Health Survey.

OBJECTIVE: To analyze the association of depression with various health behaviors and to verify if they differ according to gender or income. METHODS: This is a cross-sectional study based on data of 65,803 Brazilian adults (18-59 years old) interviewed in the National Health Survey, conducted in 2019. Presence or absence of depression was evaluated using the Patient Health Questionnaire (PHQ)-9. The prevalence of smoking, alcohol consumption, physical activity, sedentary lifestyle and food indicators were estimated according to the presence of depression. Stratified analyses were made according to sex and income, and prevalence ratios were estimated using the Poisson Regression. RESULTS: We found a significant association between depression and all indicators studied, except occasional alcohol consumption. Depression was associated with heavy episodic drinking and insufficient consumption of fruits and vegetables only in women. In men, the associations of depression with sedentary lifestyle and with being a former smoker were stronger than in women. The occasional consumption of alcohol was more prevalent only in men without depression. The analysis stratified by income showed that the association of depression with physical inactivity is stronger in the higher-income group, while with heavy episodic drinking is only significant in the lower-income stratum. CONCLUSION: The results point to the need to consider mental health in programs aimed at reducing harmful health behaviors and the specificity of sociodemographic groups.

Association between health behaviors and depression: findings from the 2019 Brazilian National Health Survey / Associação entre comportamentos de saúde e depressão: resultados da Pesquisa Nacional de Saúde de 2019

ABSTRACT Objective: To analyze the association of depression with various health behaviors and to verify if they differ according to gender or income. Methods: This is a cross-sectional study based on data of 65,803 Brazilian adults (18-59 years old) interviewed in the National Health Survey, conducted in 2019. Presence or absence of depression was evaluated using the Patient Health Questionnaire (PHQ)-9. The prevalence of smoking, alcohol consumption, physical activity, sedentary lifestyle and food indicators were estimated according to the presence of depression. Stratified analyses were made according to sex and income, and prevalence ratios were estimated using the Poisson Regression. Results: We found a significant association between depression and all indicators studied, except occasional alcohol consumption. Depression was associated with heavy episodic drinking and insufficient consumption of fruits and vegetables only in women. In men, the associations of depression with sedentary lifestyle and with being a former smoker were stronger than in women. The occasional consumption of alcohol was more prevalent only in men without depression. The analysis stratified by income showed that the association of depression with physical inactivity is stronger in the higher-income group, while with heavy episodic drinking is only significant in the lower-income stratum. Conclusion: The results point to the need to consider mental health in programs aimed at reducing harmful health behaviors and the specificity of sociodemographic groups.

RESUMO: Objetivo: Analisar a associação da depressão com comportamentos de saúde e verificar se as associações diferem segundo sexo e renda. Métodos: Estudo transversal com dados de 65.803 adultos brasileiros (18-59 anos) da Pesquisa Nacional de Saúde realizada em 2019. A presença de depressão foi avaliada com o uso do Patient Health Questionnaire-9 (PHQ-9). As prevalências de tabagismo, consumo de álcool, atividade física, sedentarismo e indicadores de alimentação foram estimadas segundo a presença de depressão. Foram desenvolvidas análises estratificadas por sexo e renda e estimadas as razões de prevalência com a regressão de Poisson. Resultados: Verificou-se associação significativa da depressão com todos os indicadores estudados, exceto com o consumo eventual de álcool. Apenas nas mulheres a depressão se mostrou associada com heavy episodic drinking e com o consumo insuficiente de frutas, legumes e verduras. Nos homens, as razões de prevalência das associações de depressão com sedentarismo e com ser ex-fumante foram mais elevadas de que nas mulheres e apenas nos homens o consumo eventual de álcool foi mais prevalente naqueles sem depressão. A análise estratificada segundo a renda mostrou que a associação da depressão com a inatividade física foi mais forte no segmento de renda superior e a associação com heavy episodic drinking só foi significativa no estrato de renda inferior. Conclusão: Os resultados apontam a necessidade de considerar a saúde mental nos programas que visam à redução de comportamentos nocivos à saúde e também de levar em conta as especificidades dessas associações nos diferentes estratos sociodemográficos.

Índices larvais de Aedes aegypti e incidência de dengue: um estudo ecológico no Estado do Rio de Janeiro, Brasil / Aedes aegypti larval indices and dengue incidence: an ecological study in the state of Rio de Janeiro, Brazil / Índices larvales de Aedes aegypti e incidencia de dengue: un estudio ecológico en el estado de Río de Janeiro, Brasil

Resumo: A circulação simultânea da dengue, Zika e chikungunya impõe desafios importantes para o Brasil, que em decorrência das mudanças climáticas e outros fatores associados, estas arboviroses podem expor mais de 2 bilhões de pessoas no mundo, segundo a Organização Mundial da Saúde. A principal estratégia dos programas de controle do Aedes aegypti baseia-se no Levantamento de Índice Rápido para o Ae. aegypti (LIRAa), um inquérito amostral no qual o Índice de Infestação Predial (IIP) obtido é utilizado para priorizar áreas de intervenção. Neste estudo, analisou-se o desempenho do LIRAa quanto à sua sensibilidade na previsão de epidemias de dengue em municípios do Estado do Rio de Janeiro, em anos considerados epidêmicos. Foram obtidas as taxas de incidência (TI) por município nos anos de 2011, 2012, 2013, 2015 e 2016, e os IIP de outubro dos anos anteriores. Foram elaborados diagramas de dispersão, visando à análise exploratória e à visualização gráfica da relação entre as referidas variáveis, assim como análises de correlação de Spearman entre o IIP e o Índice de Breteau (IB) de cada ano, a fim de estimar a qualidade do LIRAa. A análise comparativa dos valores dos IIP e as respectivas TI no período indicou correlação significativa entre estas variáveis apenas em 2011/2012 (rs = 0,479; p < 0,01). Adicionalmente, foi observada correlação entre os IIP e IB. É urgente repensar os parâmetros estabelecidos pela metodologia LIRAa e investir em metodologias alternativas de vigilância entomoepidemiológica, que mensurem de forma confiável o risco de transmissão no território e assim delinear estratégias mais efetivas para o controle dessas arboviroses.

Abstract: The simultaneous circulation of dengue, Zika, and chikungunya poses major challenges for Brazil. Due to climate changes and other associated factors, more than two billion people in the world may be exposed to these arbovirus infections, according to the World Health Organization. The principal strategy for Aedes aegypti control programs is based on the Infestation Index Rapid Survey for Ae. aegypti (LIRAa), a sample survey in which the Building Infestation Index (BII) is used to prioritize areas for intervention. This study analyzed the performance of LIRAa in terms of its sensitivity for predicting dengue epidemics in municipalities in the state of Rio de Janeiro in epidemic years. Incidence rates per municipality for the years 2011, 2012, 2013, 2015, and 2016, plus the BII in October of the previous years. Scatterplots were created, aimed at an exploratory analysis and graphic visualizations of the relationship between the above-mentioned variables, as well as analyses of the Spearman correlation between the BII and the Breteau Index for each year, aimed at estimating the quality of the LIRAa. Comparative analysis of the values for the BII and the respective incidence rates in the period only indicated significant correlation between these variables in 2011/2012 (rs = 0.479; p < 0.01). There was also a correlation between BII and Breteau Index. It is urgent to rethink the parameters established by the LIRAa methodology and invest in alternative methodologies in entomological and epidemiological surveillance that reliably measure transmission risk in the territory and thus design more effective strategies to control these arbovirus infections.

Resumen: La circulación simultánea del dengue, Zika y chikungunya impone desafíos importantes para Brasil, que, a consecuencia del cambio climático y otros factores asociados, pueden estar expuestas a estas arbovirosis más de 2 billones de personas en el mundo, según la Organización Mundial de la Salud. La principal estrategia de los programas de control del Aedes aegypti se basa en el Levantamento de Índice Rápido para el Ae. aegypti (LIRAa), una encuesta de muestreo en la que el Índice de Infestación de Edificios (IIP) obtenido es utilizado para priorizar áreas de intervención. En este estudio se analizó el desempeño del LIRAa, en cuanto a su sensibilidad en la previsión de epidemias de dengue en municipios del estado de Río de Janeiro, durante años considerados epidémicos. Se obtuvieron tasas de incidencia (TI) por municipio de los años de 2011, 2012, 2013, 2015 y 2016, y los IIP de octubre de años anteriores. Se elaboraron diagramas de dispersión, visando el análisis exploratorio y visualización gráfica de la relación entre las referidas variables, así como análisis de correlación de Spearman entre el IIP y el Índice de Breteau (IB) de cada año, con el objetivo estimar la calidad del LIRAa. El análisis comparativo de los valores de los IIP y las respectivas TI en el período indicó correlación significativa entre esas variables solamente en 2011/2012 (rs = 0,479; p < 0,01). Asimismo, se observó correlación entre los IIP e IB. Es urgente repensar los parámetros establecidos por la metodología LIRAa, e invertir en metodologías alternativas de vigilancia entomoepidemiológica, que midan de forma confiable el riesgo de transmisión en el territorio y así delinear estrategias más efectivas para el control de esas arbovirosis.

Difficulty producing high-pitched sounds in singing: correlations with laryngostroboscopy and electromyographic findings / Dificuldade na produção de sons agudos no canto: correlações com a laringoestroboscopia e achados eletromiográficos

Abstract Introduction: Difficulties or limitations in producing high-pitched sounds while singing may be due to the vocal technique used or organic factor. The observation of such difficulty or limitation by singing teachers is one of the main reasons affected individuals are referred to otolaryngologists. Objective: To evaluate the laryngostroboscopic and electromyographic changes in the cricothyroid muscles of singers with difficulties or limitations producing high-pitched sounds. Methods: This is a cross-sectional study. Ten singers with difficulty producing high-pitched sounds underwent voice, laryngostroboscopic, and electromyographic evaluations. Results: None of the evaluated singers presented signs of impairment of the superior laryngeal nerve on laryngostroboscopy. The electromyographic findings for the cricothyroid muscle were normal for all singers. Six singers presented vocal fold lesions, seven had signs suggestive of laryngopharyngeal reflux, and two presented vascular changes. Conclusion: No signs suggestive of superior laryngeal nerve paresis or paralysis were observed on laryngostroboscopy and electromyography of the cricothyroid muscle in singers with difficulties or limitations producing high-pitched sounds. The presence of vocal fold lesions should be investigated in this population.

Resumo Introdução: Dificuldades ou limitações na produção de sons agudos durante o canto podem ser decorrentes da técnica vocal utilizada ou de fatores orgânicos. A observação dessa dificuldade ou limitação pelos professores de canto é uma das principais razões pelas quais os indivíduos afetados são encaminhados aos otorrinolaringologistas. Objetivo: Avaliar as alterações laringoestroboscópicas e eletromiográficas no músculo cricotireóideo de cantores com dificuldades ou limitações na produção de sons agudos. Método: Estudo de coorte transversal. Dez cantores com dificuldade de produzir sons agudos foram submetidos a avaliações de voz, laringoestroboscópicas e eletromiográficas. Resultados: Nenhum dos cantores avaliados apresentou sinais de comprometimento do nervo laríngeo superior na laringoestroboscopia. Os achados eletromiográficos para o músculo cricotireóideo foram normais para todos os cantores. Seis cantores apresentaram lesões nas pregas vocais, sete apresentaram sinais sugestivos de refluxo laringofaríngeo e dois apresentaram alterações vasculares. Conclusão: Não foram observados sinais sugestivos de paresia ou paralisia do nervo laríngeo superior na laringoestroboscopia e eletromiografia do músculo cricotireóideo em cantores com dificuldades ou limitações na produção de sons agudos. A presença de lesões nas pregas vocais deve ser investigada nessa população.

O luto nos tempos da COVID-19: desafios do cuidado durante a pandemia / Grief in the times of COVID-19:challenges to mental health care during the pandemics / El duelo en los tiempos de COVID-19: desafios para la atención en salud mental durante la pandemia / Le deuil aux temps de la COVID-19: les défis des soins de santé mentale durant la pandémie

Este artigo pretende discutir aspectos universais e peculiares da vivência de luto no contexto da pandemia por COVID-19, a partir da escuta clínica de familiares que perderam seus parentes que se encontravam internados. O trajeto para atingir este objetivo inicia-se com a descrição do espaço de escuta fornecido pelo APEM-COVID no Hospital de Clínicas da Unicamp; segue com uma apresentação sobre o luto na perspectiva de alguns reconhecidos autores e continua discutindo elementos observados no processo de luto inserido numa conjuntura sem precedente na história recente. As falas recortadas dos atendimentos dialogam e desafiam aspectos teóricos, pelo inédito do momento em que se inserem, valorizando a importância do cuidado ofertado e seu potencial de construção de saber.

The present article discusses universal and particular aspects of the mourning process during the COVID-19 pandemic. It is based on clinical listening of families who lost relatives at our inpatient service. First, we describe the type of care offered by the APEM-COVID group at the General Hospital of the University of Campinas. Then we present different theoretical approaches to grieving and finally discuss specific elements of grief noticed during the pandemic. Excerpts extracted from clinical appointments relate to and challenge theoretical aspects, shedding light on the importance of the specific type of care provided to patients and its potential to build knowledge.

Cet article discute quelques aspects universels et particuliers de l'expérience du deuil dans le contexte de la pandémie de COVID-19 à partir de l'écoute clinique de membres de famille de patients internés décédés. En premier lieu, on décrit l'espace d'écoute que le groupe APEM-COVID offre aux patients et à leurs familles à l'Hôpital des Cliniques de l'Université de Campinas. Ensuite, de différentes perspectives sur le deuil d'auteurs renommés sont présentées et pour conclure, on discute les éléments identifiés du deuil lors de ce moment si tragique et sans précédent dans l'histoire mondiale récente. Les discours des proches défient souvent les aspects théoriques dû à l'inédit du moment dans lesquels ils sont insérés, ce qui souligne l'importance des soins offerts et leur potentiel pour la construction du savoir.

El presente artículo pretende discutir aspectos universales y particulares del duelo ocasionado por la pandemia de COVID-19, tomando como punto de partida la escucha clínica de familiares de pacientes que murieron durante la hospitalización. Empezaremos con la descripción del espacio de escucha, ofrecido por el grupo APEM-COVID, en el Hospital de Clínicas de la Universidad de Campinas; enseguida, presentaremos conceptos de duelo en la perspectiva de autores muy reconocidos; y por fin discutiremos los elementos del proceso de duelo durante la presente coyuntura de una pandemia sin precedentes en la historia reciente de la humanidad. Aspectos teóricos son desafiados por la realidad invocada en las palabras de los familiares escuchados y se vuelve evidente la importancia del cuidado ofrecido, así como el potencial de construir conocimiento acerca del duelo durante situaciones extremas.

Resistant starch supplementation effects on plasma indole 3-acetic acid and aryl hydrocarbon receptor mRNA expression in hemodialysis patients: Randomized, double blind and controlled clinical trial / Efeitos da suplementação de amido resistente no ácido indol-3-acético plasmático e na expressão do mRNA do receptor aril-hidrocarboneto em pacientes em hemodiálise: ensaio clínico randomizado, duplo-cego e controlado

ABSTRACT Introduction: Gut microbiota imbalance is linked to high uremic toxins production such as indole-3-acetic acid (IAA) in chronic kidney disease patients. This toxin can activate the aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor involved with inflammation. Strategies to restore gut microbiota balance can be associated with reduced production of IAA and its deleterious effects. This study aimed to evaluate prebiotic resistant starch (RS) supplementation effects on IAA plasma levels and AhR mRNA expression in CKD patients on hemodialysis (HD). Methods: This randomized, double-blind and placebo-controlled clinical trial evaluated forty-two stable HD patients allocated in RS (n=22) or placebo (n=20) groups. Patients received, alternately, cookies and sachets containing 16 g/day of RS (Hi-Maize 260®) or manioc flour for four weeks. Fasting pre-dialysis blood samples were collected and IAA plasma levels measured by high performance liquid chromatography. Peripheral blood mononuclear cells were isolated and processed for AhR and nuclear factor kappa B (NF-κB) mRNA expression analyzes by quantitative real-time PCR. Anthropometric and biochemical parameters, as well as food intake were also evaluated. Results: Thirty-one patients completed the study, 15 in the RS group and 16 in the placebo group. Although there was no significant alteration in IAA plasma levels, neither in AhR mRNA expression and NF-κB mRNA expression after RS supplementation, a positive correlation (r=0.48; p=0.03) was observed between IAA plasma levels and AhR expression at baseline. Conclusion: Even though prebiotic RS supplementation did not influence IAA levels or AhR expression, their positive association reinforces a possible interaction between them.

RESUMO Introdução: O desequilíbrio da microbiota intestinal associa-se à alta produção de toxinas urêmicas tais como ácido indol-3-acético (AIA), em renais crônicos. Essa toxina ativa o receptor aril hidrocarboneto (AhR) - fator de transcrição ativado por ligante, na inflamação. Restaurar o equilíbrio da microbiota intestinal associa-se à produção reduzida de AIA e efeitos deletérios. Avaliamos os efeitos da suplementação de amido resistente prebiótico (AR) sobre AIA sérico e expressão de AhR mRNA em renais crônicos em HD. Métodos: Estudo clínico randomizado, duplo-cego, controlado por placebo, com 42 pacientes em HD, nos grupos AR (n = 22) ou placebo (n = 20). Os pacientes receberam, alternadamente, biscoitos e sachês com 16 g/dia de AR ou polvilho - 4 semanas. Coletamos amostras de sangue em jejum pré-diálise e medimos níveis séricos de AIA por cromatografia líquida de alta eficiência. Isolamos e processamos as células mononucleares do sangue periférico para avaliar expressão AhR mRNA e NF-κB por PCR quantitativo em tempo real. Avaliamos parâmetros antropométricos, bioquímicos e ingestão alimentar. Resultados: 31 pacientes, 15 AR e 16 no placebo. Apesar de não apresentarem alteração significativa nos níveis de AIA, nas expressões de AhR ou NF-κB mRNA pós- suplementação com AR, foi verificada uma correlação positiva (r = 0,48; p = 0,03) entre AIA sérico e expressão de AhR na linha basal. Conclusão: Embora a suplementação com o prebiótico de AR não tenha influenciado os níveis de AIA ou a expressão de AhR, sua associação positiva reforça possível interação entre eles.