RESUMO
Three tocopherol analogues methoxytocopherol (1), α-tocopherol (2) and γ-tocopherol (3) were isolated from the peels of Citrus unshiu Marcovich. The protective effects of the isolated compounds against tert-butyl hydroperoxide-induced hepatotoxicity in human liver-derived HepG2 cells and glutamate-induced oxidative stress in HT22-immortalised hippocampal cells were evaluated. Compounds 1-3 were significantly protective in HepG2 cells with EC50 values of 21.22 ± 2.01, 25.21 ± 2.11 and 25.25 ± 1.21 µM, respectively, and in HT22 cells, compounds 1-3 had EC50 values of 20.62 ± 1.36, 6.44 ± 1.65 and 9.52 ± 1.54 µM, respectively.
Assuntos
Citrus/química , Fígado/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/farmacologia , Tocoferóis/química , Animais , Linhagem Celular , Frutas/química , Células Hep G2 , Humanos , Camundongos , Fármacos Neuroprotetores/química , Fármacos Neuroprotetores/farmacologia , Tocoferóis/farmacologia , terc-Butil HidroperóxidoRESUMO
This study investigated the effect that some polyacetylenes and protopanaxatriol, which were isolated from heated ginseng (family Araliaceae), have on inhibiting Helicobacter pylori (HP) growth. Among the compounds tested, panaxytriol was quite effective in inhibiting HP growth with an MIC of 50 microg/ml. Ginsenoside Rh1 and protopanaxatriol weakly inhibited H+/K+-ATPase from a rat stomach.
Assuntos
Antibacterianos/farmacologia , Álcoois Graxos/farmacologia , Helicobacter pylori/efeitos dos fármacos , Panax/química , Alcinos , Animais , Antibacterianos/isolamento & purificação , Enedi-Inos , Álcoois Graxos/isolamento & purificação , ATPase Trocadora de Hidrogênio-Potássio/metabolismo , Helicobacter pylori/enzimologia , Helicobacter pylori/crescimento & desenvolvimento , Masculino , Ratos , Ratos Sprague-Dawley , Estômago/efeitos dos fármacos , Estômago/enzimologia , Estômago/microbiologia , Urease/metabolismoRESUMO
Three new butanolides, 2-(1-methoxy-11-dodecenyl)-penta-2,4-dien-4-olide (1), (2Z,3S,4S)-2-(11-dodecenylidene)-3-hydroxy-4-methylbutano lide (2) and (2E,3R,4R)-2-(11-dodecenylidene)-3-hydroxy-4-methoxy-4-methylbu tanolide (3), were isolated from the stems of Lindera obtusiloba BLUME. Their chemical structures were assigned by spectroscopic evidence. They exhibited cytotoxicity against cultured human tumor cell lines with their ED50 values ranging from 3.19 to 14.63 microg/ml.
Assuntos
4-Butirolactona/análogos & derivados , 4-Butirolactona/química , Antineoplásicos Fitogênicos/química , Plantas Medicinais/química , 4-Butirolactona/farmacologia , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Espectroscopia de Ressonância Magnética , Espectrofotometria Ultravioleta , Células Tumorais CultivadasRESUMO
In a bioassay-guided search for cytotoxic compounds from higher plants of South Korea, four new sesquiterpenes of the germacranolide type, named cardivins A (1), B (2), C (3), and D (4), have been isolated from the aerial parts of Carpesium divaricatum. Structures of these compounds were elucidated on the basis of spectroscopic techniques. Compounds 1, 2, 3, and 4 showed cytotoxicity to the human tumor cells, A-549 (nonsmall cell lung), SK-OV-3 (ovary), SK-MEL-2 (skin), XF-498 (central nervous system), and HCT-15 (colon).
Assuntos
Antineoplásicos Fitogênicos/isolamento & purificação , Plantas Medicinais/química , Sesquiterpenos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Coreia (Geográfico) , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Sesquiterpenos/farmacologia , Espectrofotometria Infravermelho , Células Tumorais CultivadasRESUMO
A genuine glycoside, named ginsenoside Rh4, was isolated from Korean red ginseng (Panax ginseng C. A. Meyer) through repeated column chromatography, and its chemical structure was established to be 6-O-beta-D-glucopyranosyldammar-20(22),24-diene-3 beta,6 alpha,12 beta-triol by spectral and chemical methods. The stereochemistry of a double bond at C-20(22) of ginsenoside Rh4 was characterized as (E) from a NOESY experiment in the 1H-NMR of the aglycone. Cytotoxic activities of ginsenoside Rh4 and its aglycone against cancer cell lines were evaluated by use of the SRB method.
Assuntos
Ginsenosídeos , Panax/química , Plantas Medicinais , Saponinas/isolamento & purificação , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Sequência de Carboidratos , Espectroscopia de Ressonância Magnética , Dados de Sequência Molecular , Saponinas/química , Saponinas/farmacologia , Células Tumorais CultivadasRESUMO
Two phenanthrenes were isolated from the aerial part of Dendrobium nobile Lindl. and their structures were identified to be 4,7-dihydroxy-2-methoxy-9,10-dihydrophenanthrene (1) and denbinobin (2), among which the former has been first isolated from this plant. These two compounds were found to be cytotoxic against A549 (human lung carcinoma), SK-OV-3 (human ovary adenocarcinoma), and HL-60 (human promyelocytic leukemia) cell lines. Compound 1 also showed antitumor activity on the life span of ICR mice intraperitoneally implanted with 1 x 10(6) cells of sarcoma 180.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Fenantrenos/farmacologia , Plantas Medicinais/química , Animais , Antineoplásicos Fitogênicos/química , Humanos , Masculino , Camundongos , Camundongos Endogâmicos ICR , Fenantrenos/química , Análise Espectral , Células Tumorais CultivadasRESUMO
A new cytotoxic cardenolide glycoside, euonymoside A (1), was isolated from the bark of Euonymus sieboldianus. The structure of 1 was elucidated as acovenosigenin A-3-O-beta-D-glucopyranosyl (1-->2)-alpha-L-rhamnopyranoside. The IC50 values of 1 against A549 (human lung carcinoma) and SK-OV-3 (human ovary adenocarcinoma) were 0.06 and 0.4 micrograms/ml, respectively.