RESUMO
A universal taxonomy of viruses is essential for a comprehensive view of the virus world and for communicating the complicated evolutionary relationships among viruses. However, there are major differences in the conceptualisation and approaches to virus classification and nomenclature among virologists, clinicians, agronomists, and other interested parties. Here, we provide recommendations to guide the construction of a coherent and comprehensive virus taxonomy, based on expert scientific consensus. Firstly, assignments of viruses should be congruent with the best attainable reconstruction of their evolutionary histories, i.e., taxa should be monophyletic. This fundamental principle for classification of viruses is currently included in the International Committee on Taxonomy of Viruses (ICTV) code only for the rank of species. Secondly, phenotypic and ecological properties of viruses may inform, but not override, evolutionary relatedness in the placement of ranks. Thirdly, alternative classifications that consider phenotypic attributes, such as being vector-borne (e.g., "arboviruses"), infecting a certain type of host (e.g., "mycoviruses," "bacteriophages") or displaying specific pathogenicity (e.g., "human immunodeficiency viruses"), may serve important clinical and regulatory purposes but often create polyphyletic categories that do not reflect evolutionary relationships. Nevertheless, such classifications ought to be maintained if they serve the needs of specific communities or play a practical clinical or regulatory role. However, they should not be considered or called taxonomies. Finally, while an evolution-based framework enables viruses discovered by metagenomics to be incorporated into the ICTV taxonomy, there are essential requirements for quality control of the sequence data used for these assignments. Combined, these four principles will enable future development and expansion of virus taxonomy as the true evolutionary diversity of viruses becomes apparent.
Assuntos
Bacteriófagos , Vírus , Humanos , Metagenômica , Filogenia , Vírus/genéticaRESUMO
Thaumatin-like proteins (TLPs, osmotins) form a protein family which shares a significant sequence homology to the sweet-tasting thaumatin from the plant Thaumatococcus daniellii. TLPs are not sweet-tasting and are involved in response to biotic stresses and developmental processes. Recently it has been shown using a proteomic approach that the tuber extract from Corydalis cava (Papaveraceae) contains a TLP protein. The aim of this work was to characterize the structure and expression of TLP from C. cava tubers. The results obtained using a PCR approach with degenerate primers demonstrated a coding sequence of a novel protein, named CcTLP1. It consists of 225 aa, has a predicted molecular weight of 24.2 kDa (NCBI GenBank accession no. KJ513303) and has 16 strictly conserved cysteine residues, which form 8 disulfide bridges and stabilize the 3D structure. CcTLP1 may be classified into class IX of plant TLPs. The highest CcTLP1 expression levels were shown by qPCR in the stem of the plant compared to other organs and in the medium-size plants compared to other growth phases. The results confirm that CcTLP1 is expressed during plant growth and development until flowering, with a possible defensive function against different stress conditions.
Assuntos
Corydalis/metabolismo , Proteínas de Plantas/metabolismo , Sequência de Aminoácidos , Cromatografia Líquida , Corydalis/genética , Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , Funções Verossimilhança , Modelos Moleculares , Especificidade de Órgãos/genética , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/genética , Domínios Proteicos , Espectrometria de Massas em Tandem , Transcrição GênicaRESUMO
Bacteriophage therapeutic development will clearly benefit from understanding the fundamental dynamics of in vivo phage-bacteria interactions. Such information can inform animal and human trials, and much can be ascertained from human cell-line work. We have developed a human cell-based system using Clostridium difficile, a pernicious hospital pathogen with limited treatment options, and the phage phiCDHS1 that effectively kills this bacterium in liquid culture. The human colon tumorigenic cell line HT-29 was used because it simulates the colon environment where C. difficile infection occurs. Studies on the dynamics of phage-bacteria interactions revealed novel facets of phage biology, showing that phage can reduce C. difficile numbers more effectively in the presence of HT-29 cells than in vitro. Both planktonic and adhered Clostridial cell numbers were successfully reduced. We hypothesise and demonstrate that this observation is due to strong phage adsorption to the HT-29 cells, which likely promotes phage-bacteria interactions. The data also showed that the phage phiCDHS1 was not toxic to HT-29 cells, and phage-mediated bacterial lysis did not cause toxin release and cytotoxic effects. The use of human cell lines to understand phage-bacterial dynamics offers valuable insights into phage biology in vivo, and can provide informative data for human trials.
Assuntos
Bacteriófagos/fisiologia , Clostridioides difficile/virologia , Infecções por Clostridium/microbiologia , Colo/microbiologia , Células HT29 , Interações Hospedeiro-Patógeno , Humanos , Terapia por FagosRESUMO
MAIN CONCLUSION: A novel annotated Chelidonium majus L. transcriptome database composed of 23,004 unique coding sequences allowed to significantly improve the sensitivity of proteomic C. majus assessments, which showed novel defense-related proteins characteristic to its latex. To date, the composition of Chelidonium majus L. milky sap and biosynthesis of its components are poorly characterized. We, therefore, performed de novo sequencing and assembly of C. majus transcriptome using Illumina technology. Approximately, 119 Mb of raw sequence data was obtained. Assembly resulted in 107,088 contigs, with N50 of 1913 bp and N90 of 450 bp. Among 34,965 unique coding sequences (CDS), 23,004 obtained CDS database served as a basis for further proteomic analyses. The database was then used for the identification of proteins from C. majus milky sap, and whole plant extracts analyzed using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) approach. Of about 334 different putative proteins were identified in C. majus milky sap and 1155 in C. majus whole plant extract. The quantitative comparative analysis confirmed that C. majus latex contains proteins connected with response to stress conditions and generation of precursor metabolites and energy. Notable proteins characteristic to latex include major latex protein (MLP, presumably belonging to Bet v1-like superfamily), polyphenol oxidase (PPO, which could be responsible for browning of the sap after exposure to air), and enzymes responsible for anthocyanidin, phenylpropanoid, and alkaloid biosynthesis.
Assuntos
Chelidonium/genética , Chelidonium/metabolismo , Perfilação da Expressão Gênica/métodos , Látex/metabolismo , Proteínas de Plantas/metabolismo , Proteômica/métodos , Alcaloides/metabolismo , Antioxidantes/metabolismo , Vias Biossintéticas/genética , Chelidonium/imunologia , Chelidonium/fisiologia , Regulação da Expressão Gênica de Plantas , Ontologia Genética , Anotação de Sequência Molecular , Extratos Vegetais/metabolismo , Proteínas de Plantas/genética , Metabolismo Secundário/genética , Análise de Sequência de RNA , Estresse Fisiológico/genética , Transcriptoma/genéticaRESUMO
Temperature is an important environmental factor influencing plant development in natural and diseased conditions. The growth rate of plants grown at C27°C is more rapid than for plants grown at 21°C. Thus, temperature affects the rate of pathogenesis progression in individual plants. We have analyzed the effect of temperature conditions (either 21°C or 27°C during the day) on the accumulation rate of the virus and satellite RNA (satRNA) in Nicotiana benthamiana plants infected by peanut stunt virus (PSV) with and without its satRNA, at four time points. In addition, we extracted proteins from PSV and PSV plus satRNA-infected plants harvested at 21 dpi, when disease symptoms began to appear on plants grown at 21°C and were well developed on those grown at 27°C, to assess the proteome profile in infected plants compared to mock-inoculated plants grown at these two temperatures, using 2D-gel electrophoresis and mass spectrometry approaches. The accumulation rate of the viral RNAs and satRNA was more rapid at 27°C at the beginning of the infection and then rapidly decreased in PSV-infected plants. At 21 dpi, PSV and satRNA accumulation was higher at 21°C and had a tendency to increase further. In all studied plants grown at 27°C, we observed a significant drop in the identified proteins participating in photosynthesis and carbohydrate metabolism at the proteome level, in comparison to plants maintained at 21°C. On the other hand, the proteins involved in protein metabolic processes were all more abundant in plants grown at 27°C. This was especially evident when PSV-infected plants were analyzed, where increase in abundance of proteins involved in protein synthesis, degradation, and folding was revealed. In mock-inoculated and PSV-infected plants we found an increase in abundance of the majority of stress-related differently-regulated proteins and those associated with protein metabolism. In contrast, in PSV plus satRNA-infected plants the shift in the temperature barely increased the level of stress-related proteins.
RESUMO
Efficient delivery of heterologous molecules for treatment of cells is a great challenge in modern medicine and pharmacology. Cell-penetrating peptides (CPPs) may improve efficient delivery of a wide range of macromolecular cargos, including plasmid DNA, small interfering RNA, drugs, nanoparticulate pharmaceutical carriers, and anticancer drugs. In this paper, we present the history of CPPs' discovery with special attention drawn to sequences of viral origin. We also describe different CPP families with regard to their physicochemical properties and numerous mechanisms of CPP cell uptake by direct penetration and endocytotic pathways. A detailed description is focused on formation of carrier-cargo complexes, which are needed for practical use of CPPs in medicine and biotechnology. Examples of successful application of CPPs in treatment of human diseases are also presented, including decreased tumor growth and induction of cancer cell death. Finally, we review modern design approaches to novel CPPs and prediction of their activity. To sum up, the current review presents a thorough and up-to-date knowledge of CPPs and may be a valuable source of information for researchers in pharmacology designing new therapeutic agents.
Assuntos
Peptídeos Penetradores de Células/metabolismo , Proteínas Virais/metabolismo , Animais , Antineoplásicos/administração & dosagem , Apoptose , Membrana Celular , Peptídeos Penetradores de Células/genética , Peptídeos Penetradores de Células/imunologia , Portadores de Fármacos , Vetores Genéticos , Humanos , Hipersensibilidade/imunologia , Hipersensibilidade/terapia , Imunidade Humoral , Imunidade Inata , Neoplasias/patologia , Neoplasias/terapia , Transporte Proteico , Proteínas Virais/genética , Proteínas Virais/imunologiaRESUMO
In the course of the nanoLC-nanoESI-MS/MS analysis of Chelidonium majus proteins we detected an extremely abundant 12 aa peptide (sequence: TNAENEFVTIKK). The Mascot search against NCBInr database with Viridiplantae taxonomic restriction revealed its complete identity to Unknown protein 18 from Pseudotsuga menziesii (P85925). The same sequence has also been submitted as Unknown protein 1 (P86104) from Vitis rotundifolia and as a part of the oxygen-evolving enhancer protein 1 (OEE1) from Pinus strobus (P84718). After careful inspection the record P84718 turned out to comprise a set of peptides of unconfirmed origin rather than complete protein sequence. In this paper we present extensive data indicating that the peptide in question may originate from type II cytoskeletal keratin - a common contaminant in protein samples. We found empirical evidence that it can be detected in several types of keratin-contaminated samples and its sequence is identical to one of the proteotypic peptides commonly observed for keratins. Nevertheless, the peptide has been annotated as plant protein and thus leads to data misinterpretation. We advise extreme caution when dealing with sequences of Unknown protein 18 (P. menziesii), Unknown protein 1 (V. rotundifolia) and OEE1 (P. strobus) since they are at best poorly annotated, if not artifactual. Biological significance To our knowledge, this is the first report indicating that the peptide TNAENEFVTIKK, which is identical to Unknown protein 18 from P. menziesii (P85925), Unknown protein 1 (P86104) from V. rotundifolia and a part of the oxygen-evolving enhancer protein 1 (OEE1) from P. strobus (P84718), may originate from type II cytoskeletal keratin - a common contaminant in protein samples. We found empirical evidence that it can be detected in several types of keratin-contaminated samples and its sequence is identical to one of the proteotypic peptides commonly observed for keratins. Nevertheless, the peptide has been annotated as plant protein and thus, without proper quality assessment, leads to biological misinterpretation of proteomic data.