1.
Appl Immunohistochem Mol Morphol
; 22(10): 774-6, 2014.
Artigo
em Inglês
| MEDLINE
| ID: mdl-25046232
RESUMO
This paper is dedicated to the description of superheating as a method for antigen retrieval. In our investigation, this antigen retrieval method was used on thermal plate, heating tissue sections at temperature 120° C for 90 minutes. In the research we conducted the superheating method was applied to formalin-fixed paraffin-embedded tissue sections of breast tumors. The following monoclonal antibodies were used: estrogen receptor, progesterone receptor, epithelial membrane antigen, CD34, and Ki-67. With these tested antibodies we had good staining and no loss of tissue sections during the staining process.