Traumatismos dentários na rede social Instagram: um estudo exploratório / Dental trauma on Instagram: an exploratory study / Traumatismos dentoalveolares en Instagram: un estudio exploratorio

Estudo exploratório realizado entre 14/02/2021 e 06/03/2021, visando mapear e caracterizar os perfis sobre traumatismos dentários no Instagram, analisar as publicações quanto ao teor e à qualidade do conteúdo, como também examinar a interação e a prática comunicativa. Foram incluídos 25 perfis que apresentavam baixa audiência e pequena atividade, considerando-se o número de seguidores, a frequência de atualização e o número de publicações. Dezoito perfis eram brasileiros e estavam vinculados a projetos de extensão de Instituições de Ensino Superior (IES). Realizou-se a análise de 13 posts dos três perfis ativos, e verificou-se que eles estavam em conformidade com os protocolos da Associação Internacional de Traumatologia Dentária. As práticas comunicativas eram informacionais não se configurando como espaços de aprendizagem colaborativa. Redes sociais on-line constituem-se atualmente em estratégias importantes para a divulgação científica e a participação cidadã, mas os limites e os desafios do seu uso devem ser levados em conta no planejamento das ações de comunicação institucional em saúde pública

Exploratory study made from 14/02/2021 to 06/03/2021, aiming to map and characterize the profiles about dental trauma on Instagram, besides analyzing the posts according to its content and quality, interac-tions and communicative practice. Twenty-five profiles were included and presented low audience and low activity, considering the number of followers, updating frequency and number of posts. Eighteen of these profiles were Brazilian and had links with Universities Extension Projects. Thirteen posts were analyzed on the three active profiles, and it was verified that they were in conformity to the International Association of Dental Traumatology (IADT). The communicative practices were international, not being configured as collaborative learning spaces. Social media are important strategies to scientific communication and citizen participation, but limits and challenges of its use must be considered on the planning of institutional public health communication

Estudio exploratorio realizado entre el 14/02/2021 y el 06/03/2021, con el objetivo de mapear y caracteri-zar perfiles sobre trauma dental en Instagram, además de analizar publicaciones sobre contenido y calidad del contenido, interaciones y la práctica comunicativa. Se incluyeron 25 perfiles que tenían baja audiencia y poca actividad, considerando el número de seguidores, frecuencia de actualización y número de publica-ciones. Dieciocho perfiles eran brasileños y estaban vinculados a proyectos de extensión de Instituciones de Educación Superior. Se realizó un análisis de 13 publicaciones de los tres perfiles activos y se verificó que cumplían con los protocolos de la Asociación Internacional de Traumatología Dental. Las prácticas comunicativas fueron informacionales, no configuradas como espacios de aprendizaje colaborativo. Las redes sociales constituyen estrategias importantes para la divulgación científica y la participación ciuda-dana, pero los límites y desafíos de su uso deben ser tomados en cuenta a la hora de planificar acciones de comunicación institucional en salud pública

Effectiveness of intentional replantation in managing teeth with apical periodontitis: A systematic review.

BACKGROUND: Intentional replantation (IR) to treat disease of endodontic origin remains controversial. While IR has been advocated by some clinicians as another valid treatment option, others consider it as a treatment procedure of last resort. OBJECTIVE: To systematically review and critically evaluate the effectiveness of IR compared with nonsurgical root canal treatment/retreatment or apical surgery in terms of clinical and patient-related outcomes in managing permanent teeth with apical periodontitis (AP). METHODS: A literature search of five databases (PubMed, Embase, Scopus, Web of Science and Cochrane Central register of Controlled Trials), and the grey literature (Networked Digital Library of Theses and Dissertations, Open Access Theses and Dissertations, DART-Europe E-theses Portal, British Library E-Theses Online Service), from inception to November 2021, was conducted. Randomised clinical trials, comparative clinical trials (CCTs), nonrandomised, retrospective, and prospective comparative cohort and case-control two arm studies which evaluated clinical and/or patient-related outcomes of permanent teeth with AP treated with IR compared with nonsurgical root canal treatment/retreatment or apical surgery were identified. The quality of the studies was appraised using the Cochrane risk of bias tool for randomized trials (RoB 2.0), the Robins-I for nonrandomized controlled clinical trials and the Newcastle-Ottawa scale for observational studies. Two independent reviewers were involved in the literature selection, performed the data extraction and the appraisal of the studies identified; disagreements were resolved in conjunction with a third reviewer. RESULTS: No comparative study was identified from the literature search. Four longitudinal studies (one prospective and three retrospective) were identified during the screening of titles and abstracts but were excluded after reading the full text, because only IR was evaluated (single arm studies). DISCUSSION: The available evidence on IR is mainly based on observational studies, which reported high overall mid- to long-term survival, and low complication rates. The results suggest that IR may represent an alternative treatment procedure to solve problems of endodontic origin. However, in the absence of high-quality evidence from CCTs, clinical decision-making should be on a case-by-case basis and in accordance with the clinician's experience and the patient's preference. CONCLUSION: Clinical studies showed that IR may be a treatment modality to manage problems of endodontic origin. However, clinical trials comparing the effectiveness of IR with nonsurgical root canal treatment/retreatment or apical surgery are still required given the paucity of evidence.

Imaging diagnosis of external root resorption in replanted permanent teeth

Abstract The present study aimed to evaluate the performance of cone beam computed tomography (CBCT) and digital periapical radiographs (PR) in diagnosing external root resorption (ERR) in human permanent teeth replanted after traumatic avulsion. The samples comprised 39 permanent maxillary incisors replanted after traumatic avulsion. Digital PR and CBCT images were taken from each tooth and independently examined by 2 calibrated examiners to assess the ERR activity regarding type and extension. The degrees of agreement between both imaging examinations were determined by the mean global agreement index using SPSS software. The two imaging examinations diverged greatly in the diagnosis of the type of ERR since CBCT identified more cases as inflammatory ERR and PR as replacement ERR. A discordance level of 69.2% was observed between the two methods in the diagnosis of the type of ERR when CBCT for mesial and distal (MD) surfaces was considered and 61.5% when CBCT for mesial, distal, buccal and lingual (MD/BL) was considered. Likewise, CBCT and PR differed regarding the ERR index. PR examinations classified most cases as moderate or severe (69.2%), while CBCT examinations classified more cases as mild either in the MD surfaces analysis (41.4%) or in the analysis of the MD-BL surfaces (51.3%). In conclusion, the present results highlight a discrepancy between CBCT and digital PR performance in the diagnosis of different types and extent of ERR in replanted teeth.

DNA Methylation patterns of immune response-related genes in inflammatory external root resorption

Abstract Inflammatory external root resorption (IERR) is a pathological process defined by the progressive loss of dental hard tissue, dentin, and cementum, resulting from the combination of the loss of external root protective apparatus and root canal infection. It has been suggested that healing patterns after tooth replantation may be influenced by the genetic and immunological profiles of the patients. The purpose of the present investigation was to evaluate the DNA methylation patterns of 22 immune response-related genes in extracted human teeth presenting with IERR. Methylation assays were performed on samples of root fragments showing IERR and compared with healthy bone tissue collected during the surgical extraction of impacted teeth. The methylation patterns were quantified using EpiTect Methyl II Signature Human Cytokine Production PCR Array. The results revealed significantly higher hypermethylation of the FOXP3 gene promoter in IERR (65.95%) than in the bone group (23.43%) (p < 0.001). The ELANE gene was also highly methylated in the pooled IERR sample, although the difference was not statistically significant (p= 0.054). Our study suggests that the differential methylation patterns of immune response-related genes, such as FOXP3 and ELANE, may be involved in IERR modulation, and this could be related to the presence of root canal infection. However, further studies are needed to corroborate these findings to determine the functional relevance of these alterations and their role in the pathogenesis of IERR.

Expression of Inflammatory Cytokines and Chemokines in Replanted Permanent Teeth with External Root Resorption.

INTRODUCTION: The progressive forms of inflammatory external root resorption (IERR) and replacement external root resorption (RERR) are serious complications and the main causes of tooth loss after replantation. This study aimed to investigate the expression pattern of inflammatory molecules in extracted human teeth presenting with external root resorption (ERR) after replantation. METHODS: Root fragments from 22 teeth showing IERR and 20 teeth with RERR were triturated using a homogenizer to extract inflammatory molecules. Interleukin-1ß (IL-1ß), IL-1Ra, transforming growth factor beta, IL-8/CXCL8, CCL2, CCL3, and CCL5 were measured using double-ligand enzyme-linked immunosorbent assay, and IL-2, IL-4, IL-6, IL-10, tumor necrosis factor alpha, interferon gamma, and IL-17A detection was performed using the multiplex Th1/Th2/Th17 Cytometric Bead Array kit (BD Biosciences, San Jose, CA). Cytokine and chemokine concentrations were compared in the RERR and IERR groups corrected by patients' age at the moment of extraction, survival time after replantation, and index of ERR, adopting a generalized estimation equation model. RESULTS: The IERR group showed higher levels of tumor necrosis factor alpha than the RERR group, even after correction for the index of ERR (P < .05). IL-1Ra levels were higher in the IERR group for moderate cases but higher in the RERR group for severe cases (P < .05). IL-4 concentration became higher with the increase of patients' age in the RERR group but did not vary in the IERR group (P < .05). CCL2 levels decreased with the increase of the patients' age at the moment of extraction irrespective of the type or index of ERR (P < .05). CONCLUSIONS: The present results showed differences in the immunologic profile of IERR and RERR that may be relevant to understanding the biological mechanisms underlying ERR.