RESUMO
This paper describes the optimal implementation of three newly conceived sensors for both health and environmental applications, utilizing a wide range of detection methods and complex nanocomposites. The first one is inorganic and based on matrices of calcium oxide, the second is based on protein arrays and a third one is based on Langmuir-Blodgett laccase multi-layers. Special attention was paid to detecting substances significant to the environment (such as carbon dioxide) and medicine (drug administration, cancer diagnosis and prognosis) by means of amperometric, quartz crystal microbalance with frequency (QCM_F) and quartz crystal microbalance with dissipation monitoring (QCM_D) technologies. The resulting three implemented nanosensors are described here along with proofs of principle and their corresponding applications.
Assuntos
Técnicas Biossensoriais , Dióxido de Carbono/isolamento & purificação , Nanocompostos/química , Nanotecnologia , Compostos de Cálcio/química , Monitoramento Ambiental , Humanos , Lacase/química , Óxidos/química , Técnicas de Microbalança de Cristal de QuartzoRESUMO
OBJECTIVES: Commercial titanium dental implants are coated with nanostructured TiO2. The aim of the research reported in this paper was to assess whether the TiO2 at the surface of a dental implant is sufficiently photoactive to eradicate bacteria when illuminated with low intensity light. METHODS: The photoactivity of dental implants was established by studies of the photoenhanced decomposition of Rhodamine B. In vitro studies to establish the influence of irradiating with UV light an implant that is immersed in a solution containing Escherichia Coli were performed. RESULTS: It was demonstrated that under low UV intensity irradiation, 49 microW cm(-2), bacteria are killed at a rate of approximately 650 million per cm2 of implant per minute. SIGNIFICANCE: The results indicate that illumination of dental implants with UV light may be a suitable treatment for periimplantitis.