Adlayer-mediated antibody immobilization to stainless steel for potential application to endothelial progenitor cell capture.

This work describes the straightforward surface modification of 316L stainless steel with BTS, S-(11-trichlorosilylundecanyl)-benzenethiosulfonate, a thiol-reactive trichlorosilane cross-linker molecule designed to form intermediary coatings with subsequent biofunctionalization capability. The strategy is more specifically exemplified with the immobilization of intact antibodies and their Fab' fragments. Both surface derivatization steps are thoroughly characterized by means of X-ray photoelectron spectroscopy. The antigen binding capability of both types of biofunctionalized surfaces is subsequently assessed by fluorescence microscopy. It was determined that BTS adlayers achieve robust immobilization of both intact and fragmented antibodies, while preserving antigen binding activity. Another key finding was the observation that the Fab' fragment immobilization strategy would constitute a preferential option over that involving intact antibodies in the context of in vivo capture of endothelial progenitor cells in stent applications.

Coupling of neurons with biosensor devices for detection of the properties of neuronal populations.

The in vitro detection of the neural biophysical chemistry of populations of neurons is an important emerging area of research. This critical review describes the current methodologies, challenges and future prospects for this exciting field of research. There are different classes of techniques for the study of neuron-based systems. These include devices to measure inter-neuron contact and connectivity, microelectrodes for the determination of extracellular metabolic products, and sensors employed for the evaluation of complex neuron-small molecule interactions, toxicity, and mutagenicity of anti-tumor drugs. Since the neuron is an electrogenic cell and a complex biological entity capable of effecting recognition, the main emphasis of this article will be placed on devices based on nerve-cell networks that are able to electrically detect neuron-active compounds and specific pharmacological activity. Such neuron-based devices can be used to measure numerous neurological events with a high degree of sensitivity. Examples include the influence of different neuro-active compounds on neuronal function, the effects of neurotransmitters and neuro-modulators, changes in membrane potential, transmission effects that influence the propagation of the action potential, and the manner through which neuro-chemicals can influence ion channels. Moreover, these devices posses promising potential for the testing and development of novel neuron-active drugs and fundamental neurological research to further the understanding of brain activity. The inner workings of the human mind remain largely unknown and the key to comprehending it may rely on how molecules can initiate and influence synchronous neural oscillations, and the phenomenon of resonance in neural cells. The knowledge acquired in such detailed investigations can lead to the future development of regenerative medicines, neurochips and biocomputers, intelligent prosthetic devices and new applications that integrate neurobiology with molecular electronics (69 references).