RESUMO
Vista la creciente participación de profesionales de hospitales públicos como investigadores contratados para realizar tareas patrocinadas por la industria, utilizando las instalaciones, el equipamiento y los pacientes del hospital, las instituciones deben regular y supervisar esta tarea, a fin de asegurar que los proyectos de investigación concuerden con los legítimos intereses de los pacientes, del hosital y de la comunidad que lo sostiene. La Institución debe también recibir compensación económica por estas actividades, siendo conveniente que estos recursos se vuelquen a necesidades vinculadas a la actividad docente y a proyectos de investigación huérfanos de apoyo, paliando así el problema de la tradicional escasez de recursos para estos fines en nuestro medio.
Assuntos
Humanos , Apoio à Pesquisa como Assunto/ética , Apoio à Pesquisa como Assunto/organização & administração , Composição de Medicamentos , Indústria Farmacêutica/ética , Laboratórios Hospitalares , Reagentes de Laboratório , Pessoal de Laboratório , Protocolos Clínicos/normas , PesquisaRESUMO
The anti-inflammatory activities of three quinolones, levofloxacin, moxifloxacin, and gatifloxacin, were investigated with an in vitro model of transendothelial migration (TEM). Human umbilical vein endothelial cells (HUVEC) were seeded in Transwell inserts, treated with serial dilutions of antibiotics, infected with Chlamydia pneumoniae, or stimulated with tumor necrosis factor alpha (TNF-alpha). Neutrophils or monocytes were also preincubated with serial dilutions of each antibiotic. TEM was assessed by light microscopic examination of the underside of the polycarbonate membrane, and levels of interleukin-8 (IL-8) and monocyte chemotactic protein 1 (MCP-1) were measured by enzyme-linked immunosorbent assay. In HUVEC infected with C. pneumoniae or stimulated with TNF-alpha, all fluoroquinolones significantly decreased neutrophil and monocyte TEM, compared to antibiotic-free controls. Moxifloxacin and gatifloxacin produced a significant decrease in IL-8 in C. pneumoniae-infected and TNF-alpha-stimulated HUVEC; however, moxifloxacin was the only fluoroquinolone that produced a significant decrease in MCP-1 levels under both conditions. Results from this study indicate similarities in the anti-inflammatory activities of these fluoroquinolones, although no statistically significant decrease in chemokine secretion was observed when levofloxacin was used. Mechanisms of neutrophil and monocyte TEM inhibition by fluoroquinolone antibiotics are unknown but may be partially due to inhibition of IL-8 and MCP-1 production, respectively.
Assuntos
Anti-Infecciosos/farmacologia , Chlamydia , Células Endoteliais/citologia , Fluoroquinolonas/farmacologia , Fagócitos/efeitos dos fármacos , Pneumonia Bacteriana/patologia , Fator de Necrose Tumoral alfa/farmacologia , Compostos Aza/farmacologia , Movimento Celular/efeitos dos fármacos , Quimiocinas/biossíntese , Células Endoteliais/efeitos dos fármacos , Gatifloxacina , Humanos , Levofloxacino , Monócitos/efeitos dos fármacos , Moxifloxacina , Neutrófilos/efeitos dos fármacos , Ofloxacino/farmacologia , Quinolinas/farmacologia , Estimulação Química , Veias Umbilicais/citologia , Veias Umbilicais/patologiaRESUMO
This study investigated the potential anti-inflammatory activity of 3 macrolide antibiotics, clarithromycin, roxithromycin, and azithromycin, in an in vitro model of transendothelial migration (TEM). Human umbilical vein endothelial cells (HUVECs) were seeded in Transwell inserts, treated with serial dilutions of the antibiotics, and infected with Chlamydia pneumoniae or stimulated with tumor necrosis factor (TNF)-alpha. In HUVECs infected with C. pneumoniae or stimulated with TNF-alpha, both azithromycin and roxithromycin caused significant decreases in neutrophil and monocyte TEM, compared with antibiotic-free controls. Clarithromycin had no detectable effect in either group. Azithromycin caused significant decreases in interleukin (IL)-8 and monocyte chemotactic protein (MCP)-1, whereas roxithromycin significantly decreased IL-8. This study indicates heterogeneity in the anti-inflammatory activity of these antibiotics. Mechanisms of monocyte and neutrophil TEM inhibition by azithromycin and roxithromycin are unclear but may be partially due to inhibition of IL-8 and MCP-1 production.
Assuntos
Antibacterianos/farmacologia , Movimento Celular/efeitos dos fármacos , Chlamydophila pneumoniae/fisiologia , Endotélio Vascular/microbiologia , Monócitos/fisiologia , Neutrófilos/fisiologia , Células Cultivadas , Quimiocina CCL2/metabolismo , Infecções por Chlamydophila/microbiologia , Endotélio Vascular/citologia , Endotélio Vascular/efeitos dos fármacos , Humanos , Interleucina-8/metabolismo , Macrolídeos , Fator de Necrose Tumoral alfa/farmacologiaRESUMO
Se analizaron los niveles serológicos de anticuerpos IgG e IgA contra Chlamydia pneumoniae en 87 pacientes coronarios versus 28 controles con coronarias angiográficamente normales. La mayoría de los controles tuvieron títulos bajos de anticuerpos IgG e IgA. De los que padecían aterosclerosis coronaria, el 69 por ciento tenían títulos medianos o altos de IgG y el 32 por ciento de ellos títulos altos de IgA. Estas determinaciones demuestran que también en nuestro medio hay una relación significativa entre infección por Chlamydia pneumoniae y aterosclerosis coronaria
Assuntos
Humanos , Masculino , Adulto , Feminino , Pessoa de Meia-Idade , Infecções por Chlamydia , Chlamydophila pneumoniae , Doença da Artéria Coronariana , Anticorpos Anti-Idiotípicos , Argentina , Doença das CoronáriasRESUMO
Se describe el cuadro clínico de una paciente con enfermedad de Hand-Schuller-Christian, quien entre los dos y cinco años de edad desarrolla diabetes insípida, exoftalmos y alteraciones osteolíticas, tríada diagnóstica de la enfermedad. Además de importante compromiso visceral, presentaba en las zonas de extensión lesiones eritematoescamosas clínicamente compatibles con lesiones cutáneas de histiocitosis, pero que histopatológicamente correspondieron a eccematide psoriasiforme. Se efectúa una revisión de las manifestaciones cutáneas de la enfermedad de Hand-Schuller-Christian, de su histopatología y de la naturaleza de la enfermedad