Impact of different welding techniques on biological effect markers in exhaled breath condensate of 58 mild steel welders.

Total mass and composition of welding fumes are predominantly dependent on the welding technique and welding wire applied. The objective of this study was to investigate the impact of welding techniques on biological effect markers in exhaled breath condensate (EBC) of 58 healthy welders. The welding techniques applied were gas metal arc welding with solid wire (GMAW) (n=29) or flux cored wire (FCAW) (n=29). Welding fume particles were collected with personal samplers in the breathing zone inside the helmets. Levels of leukotriene B(4) (LTB(4)), prostaglandin E(2) (PGE(2)), and 8-isoprostane (8-iso-PGF(2α)) were measured with immunoassay kits and the EBC pH was measured after deaeration. Significantly higher 8-iso-PGF(2α) concentrations and a less acid pH were detected in EBC of welders using the FCAW than in EBC of welders using the GMAW technique. The lowest LTB(4) concentrations were measured in nonsmoking welders applying a solid wire. No significant influences were found in EBC concentrations of PGE(2) based upon smoking status or type of welding technique. This study suggests an enhanced irritative effect in the lower airways of mild steel welders due to the application of FCAW compared to GMAW, most likely associated with a higher emission of welding fumes.

Considerations for the design and technical setup of a human whole-body exposure chamber.

Exposures to air contaminants, such as chemical vapors and particulate matter, pose important health hazards at workplaces. Short-term experimental exposures to chemical vapors and particles in humans are a promising attempt to investigate acute effects of such hazards. However, a significant challenge in this field is the determination of effects of co-exposures to more than one chemical or mixtures of chemical vapors and/or particles. To overcome such a challenge, studies have to be conducted under standardized exposure characterization and real time measurements, if possible. A new exposure laboratory (ExpoLab) was installed at IPA, combining sophisticated engineering designs with new analytical techniques, to fulfill these requirements. Low-dose as well as high-dose exposure scenarios are achieved by means of a calibration-gas-generator. Exposure monitoring can be carried out with a high performance real time mass spectrometer and other suitable analyzers (e.g. gas chromatograph). Numerous automated security facilities guarantee the physical integrity of the volunteers, and the waste atmosphere is removed using either charcoal filtration or catalytic post-combustion. Measurements of sulfur hexafluoride, carbon dioxide, aniline and carbon black are presented to demonstrate the performance of the exposure unit with respect to the temporal and spatial stability of generated atmospheres. The variations of generated contents in the atmospheres at steady state are slightly higher than the measurement precision of the analyzers (the typical standard deviation of generated atmospheres is < 2%). The technical components of ExpoLab and its monitoring systems ensure high quality standards in validity and reliability of generating and measuring exposure atmospheres.

Use of a calibration gas generator for irritation threshold assessment and as supplement of dynamic dilution olfactometry.

Human odor and mucosal membrane irritation thresholds are used as criteria for assessing air quality in occupational and environmental settings. Unfortunately, reported threshold values still differ by several orders of magnitude rendering most standard compilations of little practical utility. Thus, in view of the need to repeat odor threshold measurements with a reliable methodology, a new technical approach based on original equipment manufacturer integrated solutions is presented. To test applicability, a calibration gas generator was used to continuously generate a fixed odor vapor concentration. Different dilution steps were realized by coupling to a purchasable olfactometer. Comparison with the "standard," that is, odor stimulus supply via sample bags revealed good correspondence. As a second step, the calibration gas generator was used to generate rapid changes in stimulus concentration between consecutive trials. Irritation thresholds were measured with an ascending series of ammonia concentrations generated from an aqueous solution. The obtained thresholds lay within the range previously reported. The introduced technology enables quick and reliable odor stimulus generation and provides flexibility in choosing the optimal start concentration, the step-size between dilutions, and the range of stimulus concentrations. Errors from usage of rotameters or sample bags can be avoided.