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2.
J Prev Med Hyg ; 59(2): E139-E144, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30083621

RESUMO

INTRODUCTION: The appropriate use of antibiotics is a global priority in order to avoid antibiotic resistance. Up to 50% of antibiotics usage in hospital is inappropriate (e.g. prolonged surgical prophylaxis, "defensive medicine" approach). In 2015, at the Ferrara University Hospital, an antimicrobial stewardship intervention to reduce antimicrobial prescription at the time of hospital discharge in patients at risk of surgical site infection was implemented. This programme included: update meetings for health professionals, focused meetings for critical wards, reviews of some surgical prophylaxis protocols, recommendations to reduce broad-spectrum antimicrobials use, and planning of an audit. The purpose of this study has been to evaluate the effect of this antimicrobial stewardship programme. METHODS: To evaluate the effect of this intervention, a study has been carried out including inpatients in surveillance for surgical site infection who had surgery during the last quarter of 2014 (pre-intervention group; 461 patients) and of 2015 (post-intervention group; 532 patients). RESULTS: The proportion of patients with prescription of at least one antimicrobial at discharge decreased from 33% to 24.4% (p = 0.002). The most prescribed categories of antimicrobials in both groups were the combination of penicillins with beta-lactamase inhibitors (with prescription rate reduced from 21.9% to 18%; p = 0.13) and fluoroquinolones (from 8.2% to 3.2%; p < 0.001). CONCLUSIONS: This statistically significant reduction in antimicrobial prescription after the intervention was registered without a change in surgical site infections rate (from 3.5% to 3.2%; p = 0.08). Therefore, this intervention was effective in reducing the antimicrobial prescription at discharge, without affecting patients' safety.


Assuntos
Antibacterianos/administração & dosagem , Gestão de Antimicrobianos , Resistência Microbiana a Medicamentos , Hospitais Universitários , Alta do Paciente , Infecção da Ferida Cirúrgica/prevenção & controle , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Itália , Masculino , Pessoa de Meia-Idade , Vigilância da População , Sistema de Registros
3.
Life Sci ; 164: 15-22, 2016 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-27629493

RESUMO

AIMS: Ferutinin is a diaucane sesquiterpene with a high estrogenic activity. Since ferutinin is able to enhance osteoblastic differentiation of human amniotic fluid stem cells (hAFSCs), the aim of this study was to evaluate the role of the estrogen receptors α (ERα) and G-protein coupled receptor 30 (GPR30) in ferutinin-mediated osteoblastic differentiation. Moreover, it was investigated if MEK/ERK and PI3K/Akt signaling pathways are involved in ferutinin-induced effects. MAIN METHODS: hAFSCs were cultured in a standard medium or in an osteoblastic medium for 14 or 21days and ferutinin was added at 10-8M. Immunofluorescence techniques and Western-blot 21analysis were used to study estrogen receptors and signaling pathways. KEY FINDINGS: In both undifferentiated and differentiated hAFSCs we identified ERα and GPR30 with a nuclear or cytoplasmatic localization, respectively. The presence of ferutinin in the osteoblastic medium leads to an increase in ERα expression. To dissect the role of estrogen receptors, MPP and G15 were used to selectively block ERα and GPR30, respectively. Notably, ferutinin enhanced osteoblastic differentiation in cells challenged with G15. Ferutinin was able to increase ERK and Akt phosphorylations with a different timing activation. These phosphorylations were antagonized by PD0325901, a MEK inhibitor, and wortmannin, a PI3K inhibitor. Both MPP and G15 inhibited the ferutinin-induced MEK/ERK and PI3K/Akt pathway activations. In the osteoblastic condition, PD0325901, but not wortmannin, reduced the expression of OPN and RUNX-2, whereas ferutinin abrogated the down-modulation triggered by PD0325901. SIGNIFICANCE: PI3K/Akt pathways seems to mediate the enhancement of hAFSCs osteoblastic differentiation triggered by ferutinin through ERα.


Assuntos
Benzoatos/farmacologia , Diferenciação Celular/efeitos dos fármacos , Diferenciação Celular/fisiologia , Cicloeptanos/farmacologia , Receptor alfa de Estrogênio/metabolismo , Receptores de Estrogênio/metabolismo , Receptores Acoplados a Proteínas G/metabolismo , Sesquiterpenos/farmacologia , Transdução de Sinais/fisiologia , Células-Tronco/citologia , Líquido Amniótico/citologia , Benzamidas/farmacologia , Compostos Bicíclicos com Pontes/farmacologia , Difenilamina/análogos & derivados , Difenilamina/farmacologia , Inibidores Enzimáticos/farmacologia , Receptor alfa de Estrogênio/genética , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Sistema de Sinalização das MAP Quinases/fisiologia , Osteoblastos/citologia , Osteoblastos/efeitos dos fármacos , Fosfatidilinositol 3-Quinases/metabolismo , Fosforilação/efeitos dos fármacos , Células-Tronco/enzimologia
4.
Placenta ; 36(1): 18-26, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25468543

RESUMO

INTRODUCTION: Human term placenta has attracted increasing attention as an alternative source of stem cells for regenerative medicine since it is accessible without ethical objections. The amniotic membrane (AM) contains at least two stem cell types from different embryological origins: ectodermal amniotic epithelial stem cells, and mesodermal mesenchymal stromal cells. Among the second group we studied the characteristics of amniotic mesenchymal cells (AMC) versus the ones enriched for the commonly used surface marker c-Kit (amniotic progenitor/stem cells-ASC), a stem cell factor receptor with crucial functions in a variety of biological systems and presents in early progenitors of different origin, as been already demonstrated in the enriched chorionic stem cells. METHODS: After isolation, cells from the amniotic membranes (amniotic cells-AC) were selected for c-Kit (ASC) and compared these cells with c-Kit unselected (AMC), evaluating the expression of other stem cell markers (Oct-4, Tra-1-81, SSEA-4), CD271 and Slug. RESULTS: Immunofluorescence analysis showed that ASC cells exhibited greater stem cell marker expression and included more CD271 and Slug positive cells. This was consistent with the interpretation that c-Kit enriched AC show greater stemness capacity compared to c-Kit unselected AMC. DISCUSSION: AMC and ASC can both differentiate into various cell types including adipogenic, osteogenic, chondrogenic, neurogenic and hepatic lineages, but the enrichment in c-Kit improved stemness and differentiation potential of ASC.


Assuntos
Âmnio/citologia , Diferenciação Celular/fisiologia , Células-Tronco Mesenquimais/citologia , Proteínas Proto-Oncogênicas c-kit/metabolismo , Células-Tronco/citologia , Âmnio/metabolismo , Células Epiteliais/citologia , Células Epiteliais/metabolismo , Feminino , Humanos , Células-Tronco Mesenquimais/metabolismo , Fator 3 de Transcrição de Octâmero/metabolismo , Placenta/citologia , Placenta/metabolismo , Gravidez , Células-Tronco/metabolismo
5.
Placenta ; 34(7): 526-35, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23643069

RESUMO

OBJECTIVE: Human term placenta (HTP) has attracted increasing attention as an alternative source of stem cells for regenerative medicine since the amniochorionic membrane harbors stem cells populations that are easily accessible, abundantly available without ethical objections. In the chorionic side of HTP we found a progenitor perivascular "niche" in which rare cells co-express Oct-4 and c-Kit. We investigated the stem cell characteristics and differentiation potential of a chorionic derived population enriched in c-Kit(+) cells and compared this to the unenriched population. STUDY DESIGN: Cells, isolated from the chorion of HTP, were expanded and enriched in c-Kit(+) cells (Chorionic Stem Cells-CSC). Histological staining, immunofluorescence, Western blot and flow cytometry were used to verify the stem cells characteristics of the populations and to compare the differentiation capability towards mesodermal and neural lineages in vitro. RESULTS: The expression of the pluripotent marker Oct-4 was greater in the CSCs compared to the unselected cells (Chorionic Cell-CC) but both Oct-4 and c-Kit expression decreased during passages. After differentiation, CSC displayed stronger chondrogenic and osteogenic potential and a greater adipogenic forming capacity compared to unselected ones. CSC differentiated better into immature oligodendrocytes while CC showed a neuronal progenitor differentiation potential. Moreover, both populations were able to differentiate in hepatogenic lineage. CONCLUSION: CSC display improved Oct-4 expression and a high differentiation potential into mesodermal lineages and oligodendrocytes.


Assuntos
Diferenciação Celular , Córion/citologia , Células-Tronco Embrionárias/metabolismo , Fator 3 de Transcrição de Octâmero/biossíntese , Proteínas Proto-Oncogênicas c-kit/biossíntese , Adulto , Linhagem da Célula , Córion/metabolismo , Células-Tronco Embrionárias/citologia , Feminino , Humanos , Mesoderma/citologia , Sistema Nervoso/embriologia , Placenta/citologia , Gravidez
6.
Rev Argent Microbiol ; 41(2): 92-6, 2009.
Artigo em Espanhol | MEDLINE | ID: mdl-19623898

RESUMO

Vancomycin-resistant enterococci (VRE) have an important impact on pediatric oncology population. The objectives of this study were: to know the prevalence of VRE intestinal colonization in oncology patients, to identify the risk factors that predispose hospitalized patients to VRE intestinal colonization, and to determine the VRE resistance profile to different antimicrobial agents. We studied all children with oncological disease aged 1 month to 16 years that had joined the protocol and had been hospitalized from October 2006 to April 2007. VRE intestinal colonization was analyzed when the patient was admitted to hospital, 72 hours later, and weekly during hospitalization. A total of 333 samples were taken from 67 patients. From these, VRE were isolated in 12 patients, with a prevalence of 17.9%. Of the 28 isolates studied, taking one per patient, 10 were Enterococcus faecium and 2 Enterococcus faecalis, both with resistance phenotype VanA (CIM90 512 microg/ml to vancomycin and CIM90 256 microg/ml to teicoplanin). The use of vancomycin (p = 0.02), duration of neutropenia greater than 7 days (p = 0.03) and prolonged hospitalization (42.8 days on average) (p = 0.0001) were risk factors significantly related to VRE colonization. We considered it necessary to carry out an epidemiological surveillance and to implement prevention and control measures.


Assuntos
Enterococcus/efeitos dos fármacos , Intestinos/microbiologia , Neoplasias/microbiologia , Resistência a Vancomicina , Adolescente , Antibacterianos/farmacologia , Argentina/epidemiologia , Criança , Pré-Escolar , Infecção Hospitalar/prevenção & controle , Farmacorresistência Bacteriana Múltipla , Enterococcus/isolamento & purificação , Feminino , Infecções por Bactérias Gram-Positivas/prevenção & controle , Hospitais Pediátricos , Humanos , Hospedeiro Imunocomprometido , Lactente , Recém-Nascido , Masculino , Neutropenia/microbiologia , Prevalência , Fatores de Risco
7.
Rev Argent Microbiol ; 40(2): 111-5, 2008.
Artigo em Espanhol | MEDLINE | ID: mdl-18705494

RESUMO

The purpose of our research was to know the frequency of microorganisms causing bacteremia and/or fungemia in oncology patients from Hospital de Niños de Córdoba, as well as to describe the antimicrobial susceptibility patterns of bacteria isolated from January 2006 to April 2007. A total of 59 bacteremia and fungemia cases in 44 patients were studied. From the total number of isolations, 45.8% were gram-negative bacilli, 35.6% were gram-positive cocci, and 18.6% were yeasts. The global distribution of the most prevalent microorganisms was the following: Klebsiella spp. 15.3%; Staphylococcus aureus and Candida parapsilosis 11.9%; coagulase-negative staphylococci 10.2%; Escherichia coli 8.5%, and Pseudomonas aeruginosa 6.8%. More than 40% (41.2%) of enterobacteria showed an extended-spectrum beta-lactamase phenotype, and 20.0% of non-fermenting gram-negative bacilli were multi-resistant to tested antibiotics, while 38.5% of Staphylococcus spp. were methicillin-resistant. In conclusion, the most prevalent microorganisms were gram-negative bacilli, and within this group, enterobacteria evidenced a higher percentage of resistance to tested antibiotics.


Assuntos
Bacteriemia/complicações , Bacteriemia/microbiologia , Fungemia/complicações , Fungemia/microbiologia , Neoplasias/complicações , Bacteriemia/epidemiologia , Criança , Pré-Escolar , Feminino , Fungemia/epidemiologia , Humanos , Masculino , Testes de Sensibilidade Microbiana , Prevalência
8.
Rev. argent. microbiol ; 40(2): 111-115, abr.-jun. 2008. tab
Artigo em Espanhol | LILACS | ID: lil-634588

RESUMO

El objetivo del presente trabajo fue conocer la distribución y frecuencia de los microorganismos causantes de bacteriemias y fungemias en los pacientes oncológicos internados en el Hospital de Niños de Córdoba, así como describir sus patrones de sensibilidad a los antimicrobianos. Se estudiaron 59 episodios de bacteriemias y fungemias ocurridos entre enero de 2006 y abril de 2007 en 44 pacientes. Del total de los aislamientos recuperados, el 45,8% fueron bacilos gram-negativos, el 35,6% cocos gram-positivos y el 18,6% levaduras. La distribución global de los microorganismos más prevalentes fue: Klebsiella spp. 15,3%; Staphylococcus aureus 11,9%; Candida parapsilosis 11,9%; estafilococos coagulasa negativos 10,2%; Escherichia coli 8,5% y Pseudomonas aeruginosa 6,8%. El 41,2% de las enterobacterias aisladas presentó un fenotipo compatible con la presencia de alguna b-lactamasa de espectro extendido, y el 20,0% de los bacilos gram-negativos no fermentadores presentó multirresistencia a los antibióticos ensayados. En cuanto a los cocos gram-positivos, el 38,5% de los Staphylococcus spp. fue resistente a meticilina. Se puede concluir que los microorganismos más prevalentes en la población estudiada fueron los bacilos gram-negativos; dentro de este grupo las enterobacterias fueron las que presentaron mayor porcentaje de resistencia a los antibióticos ensayados.


The purpose of our research was to know the frequency of microorganisms causing bacteremia and/or fungemia in oncology patients from Hospital de Niños de Córdoba, as well as to describe the antimicrobial susceptibility patterns of bacteria isolated from January 2006 to April 2007. A total of 59 bacteremia and fungemia cases in 44 patients were studied. From the total number of isolations, 45.8% were gram-negative bacilli, 35.6% were gram-positive cocci, and 18.6% were yeasts. The global distribution of the most prevalent microorganisms was the following: Klebsiella spp. 15.3%; Staphylococcus aureus and Candida parapsilosis 11.9%; coagulase-negative staphylococci 10.2%; Escherichia coli 8.5%, and Pseudomonas aeruginosa 6.8%. More than 40% (41.2%) of enterobacteria showed an extended-spectrum b-lactamase phenotype, and 20.0% of non-fermenting gram-negative bacilli were multi-resistant to tested antibiotics, while 38.5% of Staphylococcus spp. were methicillin-resistant. In conclusion, the most prevalent microorganisms were gram-negative bacilli, and within this group, enterobacteria evidenced a higher percentage of resistance to tested antibiotics.


Assuntos
Criança , Pré-Escolar , Feminino , Humanos , Masculino , Bacteriemia/complicações , Bacteriemia/microbiologia , Fungemia/complicações , Fungemia/microbiologia , Neoplasias/complicações , Bacteriemia/epidemiologia , Fungemia/epidemiologia , Testes de Sensibilidade Microbiana , Prevalência
9.
Peptides ; 27(6): 1426-33, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16321456

RESUMO

This study was designed to evaluate whether or not continuous intracerebroventricular infusion of leptin (1.5 microg/rat/24 h, for 28 days) produced different regional response on the skeleton of growing rats. Leptin reduce the accretion of total femoral bone mineral content (BMC) and density (BMD). This effect was related to a reduction of metaphyseal femur as no changes were detected in the diaphysis. Despite the reduced accretion in the volumetric of both femur and tibia compared to controls, leptin had no significant effects on the lumbar vertebrae. Urine deoxypyrydinoline and serum osteocalcin remained more elevated in the leptin-treated group as compared to controls. The results demonstrate that long-term central infusion of leptin activates bone remodeling with a negative balance. Leptin induces distinct responses in the different structure of bone and in the axial and appendicular skeleton.


Assuntos
Densidade Óssea/efeitos dos fármacos , Osso e Ossos/efeitos dos fármacos , Encéfalo/metabolismo , Leptina/administração & dosagem , Animais , Peso Corporal , Osso e Ossos/metabolismo , Leptina/metabolismo , Masculino , Osteocalcina/sangue , Peptídeos/química , Ratos , Ratos Sprague-Dawley , Fatores de Tempo , Tomografia Computadorizada por Raios X
10.
Cancer Lett ; 150(2): 119-27, 2000 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-10704733

RESUMO

In rodent cells, resistance to PALA (N-phosphonacetyl-L-aspartate) has always been found associated with amplification of the CAD gene (carbamyl-P synthetase, aspartate transcarbamylase, dihydro-orotase). We describe two PALA resistant Chinese hamster mutant cell lines in which amplification of the CAD gene was not present. The PALA resistant phenotype was stable when the cells were grown in non-selective medium. However, after prolonged growth in the presence of the same drug concentration used for selection, cells with increased CAD gene copy number and higher levels of resistance overrode the original population. In these cell populations, a heterogeneous organization of the CAD genes was revealed by fluorescence in situ hybridization on mitotic chromosomes indicating that the additional copies of the gene were generated in several ways, such as non-disjunction and breakage-fusion-bridge cycles. The clastogenic effect of PALA, evidenced as chromosomal aberrations in the cells grown in the presence of the drug, could have favored the late onset of the amplified mutants. It is tempting to speculate that, during the expansion of tumor populations, different drug resistance mechanisms, including gene amplification, could occur in succession and lead to the generation of cells highly resistant to chemotherapeutic agents.


Assuntos
Antineoplásicos/farmacologia , Aspartato Carbamoiltransferase/genética , Ácido Aspártico/análogos & derivados , Carbamoil Fosfato Sintase (Glutamina-Hidrolizante)/genética , Di-Hidro-Orotase/genética , Amplificação de Genes , Complexos Multienzimáticos/genética , Ácido Fosfonoacéticos/análogos & derivados , Animais , Ácido Aspártico/farmacologia , Células CHO , Cricetinae , Resistencia a Medicamentos Antineoplásicos , Dosagem de Genes , Mutação , Ácido Fosfonoacéticos/farmacologia
12.
Cancer Genet Cytogenet ; 105(2): 152-9, 1998 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-9723033

RESUMO

Two gastric cancer cell lines, AKG and GK2, were established from a pleural and an ascitic effusion, respectively. GK2 cells have a pseudodiploid karyotype with an add(6)(q27) chromosome in all metaphases examined. The karyotype of AKG cells is highly rearranged: FISH analysis with painting probes has shown that DNA sequences derived from single chromosomes are scattered on several (as many as eight) markers. In this cell line, the C-MYC and the K-RAS oncogenes are amplified. The organization and the copy number of the C-MYC-amplified units are different from the K-RAS units, suggesting that the two oncogenes were amplified independently. The presence of a few marker chromosomes carrying both C-MYC and K-RAS could be due to translocation events that followed the amplification.


Assuntos
Carcinoma/genética , Carcinoma/patologia , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia , Células Tumorais Cultivadas , Animais , Testes de Carcinogenicidade , Carcinoma/tratamento farmacológico , Divisão Celular , Aberrações Cromossômicas , Resistencia a Medicamentos Antineoplásicos , Feminino , Dosagem de Genes , Rearranjo Gênico , Genes myc , Genes ras , Humanos , Hibridização in Situ Fluorescente/métodos , Cariotipagem , Masculino , Camundongos , Camundongos Endogâmicos , Camundongos Nus , Pessoa de Meia-Idade , Neoplasias Gástricas/tratamento farmacológico
13.
Anticancer Res ; 15(1): 189-92, 1995.
Artigo em Inglês | MEDLINE | ID: mdl-7733632

RESUMO

In mammalian cells selected in culture for resistance to PALA the CAD gene is amplified and these cells are a widely used model system to study gene amplification. Selection of resistant mutants is routinely performed in medium supplemented with dialyzed serum, because the cytotoxic effect of PALA is reversed by uridine, which is contained in serum. We have shown that in Chinese hamster cells dipyridamole reduced uridine uptake to less than 5% with limited effect on cell survival. Moreover, in medium supplemented with complete serum and 10 microM dipyridamole the toxicity of PALA was similar to that obtained in medium containing dialyzed serum. We then used 10 microM dipyridamole to inhibit uridine uptake during selection of PALA resistant colonies and found that both the frequency and the type of mutants were as those obtained in the presence of dialyzed serum. In particular, in the five mutants tested, the mechanism of resistance to PALA was amplification of the CAD gene.


Assuntos
Ácido Aspártico/análogos & derivados , Dipiridamol/farmacologia , Resistência a Medicamentos , Ácido Fosfonoacéticos/análogos & derivados , Uridina/metabolismo , Animais , Aspartato Carbamoiltransferase/biossíntese , Ácido Aspártico/farmacologia , Transporte Biológico/efeitos dos fármacos , Células CHO , Carbamoil Fosfato Sintase (Glutamina-Hidrolizante)/biossíntese , Sobrevivência Celular/efeitos dos fármacos , Cricetinae , Cricetulus , Di-Hidro-Orotase/biossíntese , Relação Dose-Resposta a Droga , Amplificação de Genes , Cinética , Complexos Multienzimáticos/biossíntese , Mutagênese , Proteínas de Neoplasias/biossíntese , Ácido Fosfonoacéticos/farmacologia , Uridina/farmacologia
14.
Breast Cancer Res Treat ; 28(3): 251-60, 1993 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-8018954

RESUMO

A new cell line (BRC-230) was established from surgical material of primary ductal infiltrating breast carcinoma. The epithelial nature of this cell line was confirmed by ultrastructural analysis and demonstrated the retention of structural properties characteristic of the original tumor. The BRC-230 cell line induced tumor in athymic Cr1:nu/nu(CD-1)BR nude mice, it possessed an abnormal karyotype with a modal chromosome number between 60-61 with eight recurrent marker chromosomes, and it presented a doubling time of 30.5 hr. Scatchard analysis demonstrated that both primary tumor and BRC-230 cells were estrogen and progesterone receptor negative. Immunoenzymatic and radioimmunoassays showed a production of marker antigens (CEA, TPA, CA125, CA15-3, CA19-9) which was similar in the patient's serum and BRC-230 cells. The in vitro drug sensitivity assay of the cell line and of the parental tumor tissue showed overlapping results to all tested antiblastic drugs. BRC-230 cells were resistant to 4-Idroperoxy-cyclophosphamide, Idarubicinol, Mitoxantrone, Etoposide, 4'Epidoxorubicin, and Doxorubicin, showing a multiple drug resistance phenotype. Amplification or rearrangement of Her-2neu, Ha-ras, and C-myc genes was observed neither in the original tumor nor in BRC-230 cells; the mdr-1 gene was also present in a single copy. We conclude from these studies that the BRC-230 cell line maintains the same characteristics as the original tumor and may provide us with a good model to study in vitro the biology of drug resistance of breast cancer.


Assuntos
Neoplasias da Mama/patologia , Células Tumorais Cultivadas , Idoso , Animais , Anticorpos Monoclonais , Especificidade de Anticorpos , Antineoplásicos/farmacologia , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/genética , Divisão Celular/fisiologia , Aberrações Cromossômicas , DNA de Neoplasias/genética , Feminino , Humanos , Cariotipagem , Camundongos , Camundongos Nus , Microscopia Eletrônica , Transplante de Neoplasias
15.
Rev. bras. patol. clín ; 19(1): 19-22, 1983.
Artigo em Português | LILACS | ID: lil-14444

RESUMO

Em 53 soros de individuos com suspeita clinica do toxoplasmose adquirida, apresentando anticorpos IgG-anti-Toxoplasma gondii (IgG-aTg), detectados pelo teste da imunofluorescencia indireta (IFI), em titulos iguais ou maiores que 1:16, realizou-se tambem por IFI a pesquisa de IgM-anti-Toxoplasma gondii (IgM-aTg) no soro total e na fracao (eluato) obtida por separacao atraves de cromatografia de coluna em que se utilizou o Bio-gel A-5m (200 a 400 mesh).Demonstrou-se a presenca de IgM-aTg, em titulos iguais ou superiores a 1: 8, no soro total, em 13 (24,5%) e, no eluato, em 50 (94,3%) dos 53 casos estudados. Em nenhum dos 167 soros de individuos adultos sem suspeita clinica de toxoplasmose - em 103 (61,7%) dos quais a IgG-aTg era positiva - foi demonstrada a presenca de IgM-aTg, quer no soro total, quer no eluato


Assuntos
Pré-Escolar , Criança , Adolescente , Adulto , Humanos , Cromatografia em Gel , Imunofluorescência , Imunoglobulina M , Toxoplasmose
16.
Rev. bras. patol. clín ; 18(3): 64-8, 1982.
Artigo em Português | LILACS | ID: lil-8259

RESUMO

Os autores analizaram os resultados dos fracionamentos eletroforeticos das isoenzimas de CK e LD em 223 pacientes internados com suspeita de infarto agudo do miocardio no periodo de janeiro de 1978 a janeiro de 1981. Analisaram a presenca de CK MB e a inversao da relacao LD1/LD2, isoladamente e em conjunto, frente ao diagnostico definitivo de infarto agudo do miocardio, que se baseou em dados clinicos, eletrocardiograma e estudo eletroforetico da isoenzimas de CK e LD. A analise de CK MB e LD1/LD2 feita em conjunto foi a que apresentou maior especificidade (100%) e maior valor preditivo (100%).A presenca de CK MB mostrou ser o teste de maior sensibilidade (96%) e a inversao LD1/LD2 analisada isoladamente foi a que apresentou a maior percentagem de falsos positivos (2,7%). Os autores concluem que a eletroforese de isoenzimas de CK e LD quando analisadas em conjunto atingem uma especificidade e valor preditivo maximo para o diagnostico do infarto agudo do miocardio/


Assuntos
Humanos , Creatina Quinase , L-Lactato Desidrogenase , Infarto do Miocárdio , Eletroforese
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