RESUMO
Matrix metalloproteinases are a class of enzymes that play an important role in the remodeling of the extracellular matrix in development and cancer metastasis. This unit describes a set of methods--cell-mediated dissolution of type-1 collagen fibrils, direct and reverse zymography, enzyme capture based on alpha2-macroglobulin and TIMP-1 and -2, and demonstration of cryptic thiol groups in metalloproteinase precursors--that are used to characterize the functions of matrix metalloproteinases and their inhibitors.
Assuntos
Bioquímica/métodos , Metaloproteinases da Matriz/metabolismo , Animais , Colágeno/metabolismo , Expressão Gênica , Humanos , Inibidores de Metaloproteinases de Matriz , Metaloproteinases da Matriz/genética , Ratos , Coloração e Rotulagem , Tendões/metabolismo , Inibidores Teciduais de Metaloproteinases/metabolismo , alfa-Macroglobulinas/metabolismoRESUMO
Matrix metalloproteinases are a class of enzymes that play an important role in the remodeling of the extracellular matrix in development and cancer metastasis. This unit describes a set of methods-cell-mediated dissolution of type I collagen fibrils, direct and reverse zymography, enzyme capture based on a-2 macroglubulin and TIMP-1 and -2, and demonstration of crytic thiol groups in metalloproteinase precursors-that are used to characterize the functions of matrix metalloproteinases and their inhibitors.