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1.
J Eukaryot Microbiol ; 45(3): 323-33, 1998.
Artigo em Inglês | MEDLINE | ID: mdl-9627992

RESUMO

The emergent flagellum of euglenoids and trypanosomatids contained in addition to microtubules a prominent filamentous structure--the flagellar rod (paraflagellar/paraxonemal rod). Immunoblots and immunofluorescence localization using three antibodies generated against gel-isolated proteins confirmed previous studies that the Euglena flagellar rod consisted of polypeptides migrating at 66-, 69-, and 75-kD. Immunoblotting after two dimensional gel electrophoresis identified ten or more isoforms of these polypeptides. Differences in migration in acrylamide gels under nonreducing and reducing conditions suggested that the rod proteins contain intramolecular disulfide linkages. Comparative peptide mapping showed that the 66-, 69-, and 75-kD polypeptides were distinct, but related proteins, and also identified a fourth related protein migrating at 64-kD. Using antibodies against rod proteins, two overlapping cDNAs were isolated and from their sequences the cDNAs were predicted to encode 334 amino acids of the 66-kD protein; the amino acid sequence had > 65% identity to the carboxyl-terminus of the trypanosomatid flagellar rod proteins. Secondary structural prediction suggested that flagellar rod proteins contain an extended segmented coiled coil stalk and two nonhelical heads. Coiled coil appeared to be an important structural motif in the construction of flagellar rod filaments.


Assuntos
Euglena gracilis/química , Flagelos/química , Proteínas de Protozoários/química , Sequência de Aminoácidos , Animais , Anticorpos Monoclonais/imunologia , Citoesqueleto/química , DNA Complementar , Euglena gracilis/genética , Imunofluorescência , Immunoblotting , Leishmania mexicana/química , Dados de Sequência Molecular , Mapeamento de Peptídeos , Estrutura Secundária de Proteína , Proteínas de Protozoários/genética , Proteínas de Protozoários/isolamento & purificação , Alinhamento de Sequência , Trypanosoma/química
2.
J Eukaryot Microbiol ; 42(5): 570-80, 1995.
Artigo em Inglês | MEDLINE | ID: mdl-7581330

RESUMO

Proteins of the membrane skeleton of Euglena gracilis were extensively phosphorylated in vivo and in vitro after incubation with [32P]-orthophosphate or gamma-[32P] ATP. Endogenous protein threonine/serine activity phosphorylated the major membrane skeletal proteins (articulins) and the putative integral membrane protein (IP39) anchor for articulins. The latter was also the major target for endogenous protein tyrosine kinase activity. A cytoplasmic domain of IP39 was specifically phosphorylated, and removal of this domain with papain eliminated the radiolabeled phosphoamino acids and eliminated or radically shifted the PI of the multiple isoforms of IP39. In gel kinase assays IP39 autophosphorylated and a 25 kDa protein which does not autophosphorylate was identified as a threonine/serine (casein) kinase. Plasma membranes from the membrane skeletal protein complex contained threonine/serine (casein) kinase activity, and cross-linking experiments suggested that IP39 was the likely source for this membrane activity. pH optima, cation requirements and heparin sensitivity of the detergent solubilized membrane activity were determined. Together these results suggest that protein kinases may be important modulators of protein assembly and function of the membrane skeleton of these protistan cells.


Assuntos
Proteínas do Citoesqueleto/metabolismo , Euglena gracilis/enzimologia , Proteínas de Membrana/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Proteínas Tirosina Quinases/metabolismo , Proteínas de Protozoários/metabolismo , Trifosfato de Adenosina/metabolismo , Animais , Caseína Quinases , Cátions Bivalentes/farmacologia , Membrana Celular/enzimologia , Membrana Celular/metabolismo , Reagentes de Ligações Cruzadas , Euglena gracilis/metabolismo , Heparina/farmacologia , Concentração de Íons de Hidrogênio , Ponto Isoelétrico , Proteínas de Membrana/química , Papaína , Mapeamento de Peptídeos , Fosforilação , Proteínas Quinases/metabolismo , Proteínas de Protozoários/química
3.
J Cell Biol ; 110(4): 1077-88, 1990 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-2108968

RESUMO

The major integral plasma membrane protein (IP39) of Euglena gracilis was radiolabeled, peptide mapped, and dissected with proteases to identify cytoplasmic domains that bind and anchor proteins of the cell surface. When plasma membranes were radioiodinated and extracted with octyl glucoside, 98% of the extracted label was found in IP39 or the 68- and 110-kD oligomers of IP39. The octyl glucoside extracts were incubated with unlabeled cell surface proteins immobilized on nitrocellulose (overlays). Radiolabel from the membrane extract bound one (80 kD) of the two (80 and 86 kD) major membrane skeletal protein bands. Resolubilization of the bound label yielded a radiolabeled polypeptide identical in Mr to IP39. Intact plasma membranes were also digested with papain before or after radioiodination, thereby producing a cytoplasmically truncated IP39. The octyl glucoside extract of truncated IP39 no longer bound to the 80-kD membrane skeletal protein in the nitrocellulose overlays. EM of intact or trypsin digested plasma membranes incubated with membrane skeletal proteins under stringent conditions similar to those used in the nitrocellulose overlays revealed a partially reformed membrane skeletal layer. Little evidence of a membrane skeletal layer was found, however, when plasma membranes were predigested with papain before reassociation. A candidate 80-kD binding domain of IP39 has been tentatively identified as a peptide fragment that was present after trypsin digestion of plasma membranes, but was absent after papain digestion in two-dimensional peptide maps of IP39. Together, these data suggest that the unique peripheral membrane skeleton of Euglena binds to the plasma membrane through noncovalent interactions between the major 80-kD membrane skeletal protein and a small, papain sensitive cytoplasmic domain of IP39. Other (62, 51, and 25 kD) quantitatively minor peripheral proteins also interact with IP39 on the nitrocellulose overlays, and the possible significance of this binding is discussed.


Assuntos
Citoesqueleto/ultraestrutura , Euglena gracilis/análise , Proteínas de Membrana/análise , Proteínas de Protozoários/análise , Animais , Autorradiografia , Membrana Celular/ultraestrutura , Detergentes , Eletroforese em Gel Bidimensional , Eletroforese em Gel de Poliacrilamida , Euglena gracilis/ultraestrutura , Glucosídeos , Radioisótopos do Iodo , Microscopia Eletrônica , Modelos Estruturais , Peso Molecular , Mapeamento de Peptídeos , Conformação Proteica , Tripsina
4.
J Cell Biol ; 107(1): 191-200, 1988 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-3134363

RESUMO

The cellular distribution, membrane orientation, and biochemical properties of the two major NaOH-insoluble (integral) plasma membrane proteins of Euglena are detailed. We present evidence which suggests that these two polypeptides (Mr 68 and 39 kD) are dimer and monomer of the same protein: (a) Antibodies directed against either the 68- or the 39-kD polypeptide bind to both 68- and 39-kD bands in Western blots. (b) Trypsin digests of the 68- and 39-kD polypeptides yield similar peptide fragments. (c) The 68- and 39-kD polypeptides interconvert during successive electrophoresis runs in the presence of SDS and beta-mercaptoethanol. (d) The 39-kD band is the only major integral membrane protein evident after isoelectric focusing in acrylamide gels. The apparent shift from 68 to 39 kD in focusing gels has been duplicated in denaturing SDS gels by adding ampholyte solutions directly to the protein samples. The membrane orientation of the 39-kD protein and its 68-kD dimer has been assessed by radioiodination in situ using intact cells or purified plasma membranes. Putative monomers and dimers are labeled only when the cytoplasmic side of the membrane is exposed. These results together with trypsin digestion data suggest that the 39-kD protein and its dimer have an asymmetric membrane orientation with a substantial cytoplasmic domain but with no detectable extracellular region. Immunolabeling of sectioned cells indicates that the plasma membrane is the only cellular membrane with significant amounts of 39-kD protein. No major 68- or 39-kD polypeptide bands are evident in SDS acrylamide gels or immunoblots of electrophoresed whole flagella or preparations enriched in flagellar membrane vesicles, nor is there a detectable shift in any flagellar polypeptide in the presence of ampholyte solutions. These findings are considered with respect to the well-known internal crystalline organization of the euglenoid plasma membrane and to the potential for these proteins to serve as anchors for membrane skeletal proteins.


Assuntos
Euglena gracilis/ultraestrutura , Proteínas de Membrana/análise , Animais , Membrana Celular/análise , Membrana Celular/ultraestrutura , Reações Cruzadas , Eletroforese em Gel de Poliacrilamida , Euglena gracilis/análise , Imunofluorescência , Imunoensaio , Imuno-Histoquímica , Focalização Isoelétrica , Proteínas de Membrana/imunologia , Microscopia Eletrônica , Mapeamento de Peptídeos
5.
J Cell Biol ; 101(5 Pt 1): 1884-96, 1985 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-3932367

RESUMO

In this paper we show that a membrane skeleton associated with the plasma membrane of the unicellular organism Euglena consists of approximately 40 individual S-shaped strips that overlap along their lateral margins. The region of strip overlap is occupied by a set of microtubule-associated bridges and microtubule-independent bridges. Both cell form and plasma membrane organization are dependent on the integrity of this membrane skeleton. Removal of the membrane skeleton with a low-molar base results in loss of membrane form and randomization of the paracrystalline membrane interior characteristic of untreated cells. Conversely, removal of the plasma membrane and residual cytoplasm with lithium 3,5-diiodosalicylate/Nonidet P-40 yields cell ghosts that retain the form of the original cell but consist only of the membrane skeleton. Two major polypeptides of 86 and 80 KD persist in the skeleton and two other major proteins of 68 and 39 kD are associated with the plasma membrane fraction. None of these components appears to be the same as the major polypeptides (spectrins, band 3) of the erythrocyte ghost, the other cell system in which a well-defined peripheral membrane skeleton has been identified. We suggest that the articulating strips of euglenoids are not only the basic unit of cell and surface form, but that they are also positioned to mediate or accommodate surface movements by sliding, and to permit surface replication by intussusception.


Assuntos
Membrana Celular/ultraestrutura , Euglena gracilis/ultraestrutura , Animais , Fracionamento Celular/métodos , Movimento Celular , Centrifugação com Gradiente de Concentração/métodos , Euglena gracilis/fisiologia , Técnica de Fratura por Congelamento , Microscopia Eletrônica , Modelos Biológicos
6.
J Cell Biol ; 77(3): 805-26, 1978 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-98532

RESUMO

The surface of the Euglena flagellum is coated with about 30,000 fine filaments of two distinct types. The longer of these nontubular mastigonemes (about 3 micron) appear to be attached to the paraflagellar rod whereas the shorter nontubular mastigonemes (about 1.5 micron) are the centrifugally arranged portions of a larger complex, which consists of an attached unit parallel to and outside of the flagellar membrane. Units are arranged laternally in near registration and longitudinally overlap by one-half of a unit length. Rows of mastigoneme units are firmly attached to the axoneme microtubules or to the paraflagellar rod as evidenced by their persistence after removal of the flagellar membrane with neutral detergents. SDS-acrylamide gels of whole flagella revealed about 30 polypeptides, of which two gave strong positive staining with the periodic acid-Schiff (PAS) procedure. At least one of these two bands (glycoproteins) has been equated with the surface mastigonemes by parallel analysis of isolated and purified mastigonemes, particularly after phenol extraction. The faster moving glycoprotein has been selectively removed from whole flagella and from the mastigoneme fraction with low concentrations of neutral detergents at neutral or high pH. The larger glycoprotein was found to be polydisperse when electrophoresed through 1% agarose/SDS gels. Thin-layer chromatography of hydrolysates of whole flagella or of isolated mastigonemes has indicated that the major carbohydrate moiety is the pentose sugar, xylose, with possibly a small amount of glucose and an unknown minor component.


Assuntos
Euglena gracilis/ultraestrutura , Membrana Celular/ultraestrutura , Flagelos/análise , Flagelos/ultraestrutura , Glicoproteínas/análise , Humanos , Microtúbulos/ultraestrutura , Modelos Estruturais , Peptídeos/análise
7.
J Cell Biol ; 69(3): 693-715, 1976 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-818092

RESUMO

The surface complex of Euglena has been examined intact and after isolation and purification by the use of mild sonication to disrupt cells. In intact cells the surface complex (pellicle complex) is oriented in a series of parallel ridges and grooves, and possesses among other components a characteristic group of four to seven microtubules. Isolated pellicles retain the ridge and groove pattern but no microtubules are present. Isolates yielded at least three major polypeptides on SDS acrylamide gels; one or more of the polypeptides are postulated to be identical with a submembrane layer present in both intact and isolated pellicles; one polypeptide appears to be in or on the surface membrane. Antibodies directed against the isolated pellicles were conjugated directly or indirectly to fluorescein, latex spheres, or ferritin. In appropriate experiments with these antibody conjugates, it has been found that antigenic sites are immobile and that new antigenic sites (daughter strips) are inserted between parental strips in replicating cells. These results together with direct observation of daughter strips by transmission electron microscopy suggest that surface growth in Euglena occurs by intussusception. Microtubules associated with the pellicle complex are postulated to play a role in the development of new daughter strips, and possibly also in cell movements.


Assuntos
Euglena gracilis/crescimento & desenvolvimento , Microtúbulos/ultraestrutura , Proteínas/fisiologia , Antígenos , Carboidratos/análise , Divisão Celular , Fracionamento Celular , Membrana Celular/imunologia , Membrana Celular/ultraestrutura , Euglena gracilis/ultraestrutura , Imunofluorescência , Lipídeos/análise , Microtúbulos/fisiologia , Modelos Biológicos , Proteínas/análise
8.
J Cell Biol ; 50(2): 362-84, 1971 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-5123323

RESUMO

The structure, assembly, and composition of the extracellular hairs (mastigonemes) of Ochromonas are detailed in this report. These mastigonemes form two lateral unbalanced rows, each row on opposite sides of the long anterior flagellum. Each mastigoneme consists of lateral filaments of two distinct sizes attached to a tubular shaft. The shaft is further differentiated into a basal region at one end and a group of from one to three terminal filaments at the free end. Mastigoneme ontogeny as revealed especially in deflagellated and regenerating cells appears to begin by assembly of the basal region and shaft within the perinuclear continuum. However, addition of lateral filaments to the shaft and extrusion of the mastigonemes to the cell surface is mediated by the Golgi complex. The ultimate distribution of mastigonemes on the flagellar surface seems to be the result of extrusion of mastigonemes near the base of the flagellum, and it is suggested that mastigonemes are then pulled up the flagellum as the axoneme elongates. Efforts to characterize mastigonemes biochemically after isolation and purification on cesium chloride (CsCl) followed by electrophoresis on acrylamide gels have demonstrated what appear to be a single major polypeptide and several differentially migrating carbohydrates. The polypeptide is not homologous with microtuble protein. The functionally anomalous role of mastigonemes in reversing flagellar thrust is discussed in relation to their distribution relative to flagellar anatomy and to the plane of flagellar undulations.


Assuntos
Eucariotos/citologia , Flagelos/citologia , Carboidratos/análise , Núcleo Celular , Césio , Cloretos , Cloroplastos , Eletroforese Descontínua , Flagelos/análise , Complexo de Golgi , Locomoção , Morfogênese , Peptídeos/análise , Regeneração
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