RESUMO
The prototype virus for the PMV-7 serotype of avian paramyxoviruses, PMV-7 dove Tennessee/4/75 (Tn 4) and five other isolates obtained from birds of the Columbidae family, which had been shown to be distinct from PMV-1 serotype, were tested for antigenic relationships between themselves and to other avian paramyxoviruses. By serological tests and analysis of structural polypeptides the viruses appeared to be distinct from other avian paramyxoviruses. One isolate appeared to be very closely related to Tn/4. Three other isolates showed only minor relationships to these two but were very closely related to each other. However, the sixth virus, pigeon Japan/Otaru/76, showed high levels of homology in haemagglutination inhibition tests and at least one line of identity in immunodoublediffusion tests with all five of the other isolates.
Assuntos
Columbidae/microbiologia , Paramyxoviridae/classificação , Animais , Anticorpos Antivirais/imunologia , Antígenos Virais/imunologia , Eletroforese em Gel de Poliacrilamida , Testes de Inibição da Hemaglutinação , Imunodifusão , Paramyxoviridae/imunologia , Paramyxoviridae/isolamento & purificação , SorotipagemRESUMO
Nine paramyxovirus isolates obtained from penguins were tested for antigenic relationships amongst themselves and to other avian paramyxoviruses. One of the isolates was shown to be a lentogenic Newcastle disease virus (NDV), i.e., of PMV-1 serotype. By serological tests and analysis of structural polypeptides the other penguin isolates could be placed into three groups. No relationship with other avian paramyxoviruses could be determined except that six of the penguin viruses, representing two of the groups, showed reaction with a monoclonal antibody raised against NDV Ulster 2C and three of the isolates, representing one of the penguin groups, also reacted with another PMV-1 directed monoclonal antibody.
Assuntos
Aves/microbiologia , Paramyxoviridae/classificação , Animais , Regiões Antárticas , Antígenos Virais/análise , Testes de Inibição da Hemaglutinação , Imunodifusão , Peso Molecular , Paramyxoviridae/isolamento & purificação , Proteínas Virais/análiseRESUMO
A brief summary of methods for reconstruction of the external auditory meatus for whatever reason, which have been recommended and used by a number of authors, is presented. Recurrence of the atretic problem occurs in a great percentage of individuals necessitating further attempts with an even greater failure rate. In 13 ases with this problem, including congenital agenesis and/or atresia of the external auditory canal, exostectomies, and postsurgical stenosis, I have fund that surgery with concomitant use of Merocel wicks has given a "cure rate" of better than 90%. The first patient in this group has been followed for approximately two years.