RESUMO
Bioluminescence is a spectacular feature of some prokaryotes. In the present work, we address the distribution of bioluminescence among bacteria isolated from the White Sea finfishes. Luminous bacteria are widely distributed throughout the World Ocean. Many strains have been isolated and described for tropical latitudes, while Nordic seas still remain quite a white spot in studying bioluminescence of bacteria. We describe the strains related to the two main genera of luminous bacteria, Photobacterium and Aliivibrio, as well as Shewanella and Vibrio. They are related to families Vibrionaceae and Shewanellaceae of the Gammaproteobacteria class. Here, we at the first time, report the bioluminescence of the Enterobacteriaceae Kosakonia cowanii. Moreover, we applied the polyphasic approach to identify and describe the isolated microorganisms. The data on sequencing, diversity of cell fine structure, and light emission spectra at room temperature on the solid medium are discussed. The bacteria are characterized by features in their light emission spectra. It may reflect possible molecular mechanisms of bioluminescence as well as features of bacterial composition. The obtained data expands the existing body of knowledge about the bioluminescence spread among the bacteria of Nordic latitudes and provides complex information that is crucial for their precise identification.
Assuntos
Aliivibrio/genética , Enterobacteriaceae/genética , Peixes/microbiologia , Photobacterium/genética , Shewanella/genética , Vibrio/genética , Aliivibrio/isolamento & purificação , Animais , Enterobacteriaceae/classificação , Enterobacteriaceae/isolamento & purificação , Photobacterium/classificação , Photobacterium/isolamento & purificação , Filogenia , RNA Ribossômico 16S/química , RNA Ribossômico 16S/metabolismo , Shewanella/classificação , Shewanella/isolamento & purificação , Espectrometria de Fluorescência , Vibrio/classificação , Vibrio/isolamento & purificaçãoRESUMO
Activity of hexokinase and acetylcholinesterase and pyridoxal co-enzyme content of brain subcellular fractions were studied in rats, bearing sarcoma 45, after local exposure of the tumor to 20 Gy X-radiation and microwave hyperthermia. The carbohydrate metabolism was sharply inhibited while the pyridoxal coenzyme content and acetylcholinesterase activity increased.