Assuntos
Adenoma/complicações , Bócio Nodular/complicações , Doença de Graves/complicações , Doença de Hashimoto/complicações , Neoplasias da Glândula Tireoide/complicações , Adenoma/tratamento farmacológico , Antagonistas Adrenérgicos beta/uso terapêutico , Idoso , Antitireóideos/uso terapêutico , Carbimazol/uso terapêutico , Feminino , Bócio Nodular/tratamento farmacológico , Doença de Graves/sangue , Doença de Graves/tratamento farmacológico , Doença de Hashimoto/tratamento farmacológico , Humanos , Imunoglobulinas Estimuladoras da Glândula Tireoide/sangue , Propranolol/uso terapêutico , Neoplasias da Glândula Tireoide/tratamento farmacológico , Tiroxina/uso terapêuticoRESUMO
Conventional immunohistochemistry (IHC) is a widely used diagnostic technique in tissue pathology. However, this technique is associated with a number of limitations, including high inter-observer variability and the capacity to label only one marker per tissue section. This review details various highly multiplexed techniques that have emerged to circumvent these constraints, allowing simultaneous detection of multiple markers on a single tissue section and the comprehensive study of cell composition, cellular functional and cell-cell interactions. Among these techniques, multiplex Immunohistochemistry/Immunofluorescence (mIHC/IF) has emerged to be particularly promising. mIHC/IF provides high-throughput multiplex staining and standardized quantitative analysis for highly reproducible, efficient and cost-effective tissue studies. This technique has immediate potential for translational research and clinical practice, particularly in the era of cancer immunotherapy.