IgG and IgM glycosylation patterns in patients undergoing image-guided tumor ablation.

BACKGROUND: Image-guided tumor ablation is a technique whereby needle-like applicators are placed directly into solid tumors under guidance typically with computed tomography or ultrasound. Changes in IgG and IgM antibody glycosylation were studied during ablation-induced immune response to cancer, and the use of glycosylation as a biomarker for diagnosis, prognosis and disease treatment was examined. METHODS: Plasma from 27 tumor patients was collected immediately before, after and for 6 months following ablation. IgG and IgM antibodies were isolated by use high-throughput chromatography, and analyzed by hydrophilic liquid chromatography. Thorough identification of glycan structures in each chromatography peak was performed by nano-liquid chromatography electrospray ionization mass spectrometry. RESULTS: Although antibody glycosylation was found to vary with cancer type, discernable patterns of change based on the successful treatment of tumors by ablation were not identified. One patient with renal clear cell carcinoma and poor disease outcome had unexpectedly high amount of oligomannose IgG glycans during the whole period of monitoring. In contrast, IgM antibodies did not follow the same pattern. CONCLUSIONS: These findings suggest that glycosylation patterns are indicative of an immune system that is unable to prevent different types of cancer, rather than products of the immunostimulatory response to the ablation of tumor itself. Analyses of the outcome effect suggested that IgG glycosylation and IgM glycosylation are not associated with tumor ablation. GENERAL SIGNIFICANCE: Present work opens a new way for parallel determination of glycosylation changes of both IgG and IgM antibodies by use of high-throughput methods, and their future use as biomarkers for disease diagnosis and prognosis. This article is part of a Special Issue entitled "Glycans in personalised medicine" Guest Editor: Professor Gordan Lauc.

[Thyroid surgery: experience of the surgical service Ignace Deen Central University Hospital in Conakry]. / La chirurgie thyroïdienne: experience du service de Chirujrgie Generale du CHU Ignace Deen de Conakry.

Authors bring back their experience in surgery thyroïdienne patient sur192s admitted and operated in five years in tea service of General Surgery of him FALLEN Ignace Deen Conakry of. Thesis patients represented 3.13% of tea 6139 surgical interventions exercised during tea period of survey. It was about has retrospective survey of descriptive type going from January 1st, 1997 at 31 December 2001, structural one aspects epidemiological, clinic, therapeutic and histologique. Tea done operative gesture was 86 lefts loboisthmectomieseses (44.17%), 41 rights loboisthmectomieseses (21.35%), 58 thyroidectomies subtotales (30.21%), 7 thyroidectomieses total (3.65%). Tea gotten results were good in 99.48%. To slot of death has been recorded, representing 0.52%. Tea goalkeeper of tea survey was to appreciate results of surgery thyroidienne and to take out again elements of bad prognostication of this surgery in their service.

Pé diabético: epidemiologia da resistência a antimicrobianos de 61 cepas de Staphyloccocus aureus isoladas de 141 pacientes ambulatoriais / Diabetic foot: epidemiologia of the resistance isolated aureus the 61 antimicrobialses of cepas of Staphyloccocus of 141 ambulatoriais patients

Foram atendidos no Centro Integrado de Diabetes e Hipertensão do Estado do Ceará, 141 pacientes diabéticos apresentando úlceras infectadas no período de 01/03/2001 a 30/11/2002 e submetidos a um estudo microbiológico no Laboratório de Microbiologia do Departamento de Medicina Legal da Universidade Federal do Ceará. Staphyloccocus aureus foi isolado de (43, 2)dos pacientes. Das cepas de Staphyloccocus aureus 07 (11,5...

Expression of complement receptor type 1 (CR1) on erythrocytes of paracoccidiodomycosis patients.

Complement receptor type 1 (CR1) is a membrane glycoprotein that acts as a receptor for the C3b, iC3b and C4b fragments of complement. In primates, one function of erythrocytes is to promote safe clearance of immunocomplexes (IC) from the circulation through CR1. Theoretically, in diseases characterized by high levels of circulating IC, an erythrocyte CR1 (CR1/E) deficiency may favor IC deposition in tissues or facilitate inappropriate activation of leukocytes in the circulation. Depression of the cell immune response occurs in paracoccidioidomycosis (PCM), especially in the more severe cases, and is frequently associated with high serum IC levels. In the present study we quantified the number of CR1/E in patients with the acute and chronic forms of PCM before and after treatment and correlated it with serum IC levels and CD4+ and CD8+ T cell concentration in the peripheral blood of these patients. Patients with PCM, particularly those with active disease and who had received treatment for shorter periods of time, had low numbers of CR1/E. In addition, an increase in serum IC concentration and a reduction in the CD4+/CD8+ T cell ratio were observed. After treatment there was a significant increase in mean CR1/E number and a reduction in serum IC levels. In patients with the chronic form of the disease the CD4+/CD8+ T cell ratio tended to increase after treatment and was associated with increased CR1/E levels. These results suggest that the reduction in CR1/E observed in patients is a phenomenon acquired with the disease and that CR1 could play a role in the pathogenesis of PCM.