RESUMO
PURPOSE: Experimental data have demonstrated a relevant role for IL-6 in the modulation of acute ocular toxoplasmosis. Therefore, we aim to investigate the possible association between the IL-6 gene polymorphism at position -174 and toxoplasmic retinochoroiditis (TR) in humans. METHODS: Ninety-seven patients with diagnosed TR were recruited from the Uveitis Section, Federal University of Minas Gerais. For comparison, 83 healthy blood donors with positive serology for toxoplasmosis and without retinal signs of previous TR were included in the study. Genomic DNA was obtained from oral swabs of individuals and amplified using polymerase chain reaction (PCR) with specific primers flanking the locus -174 of IL-6 (-174G/C). PCR products were submitted to restriction endonuclease digestion and analysed by polyacrylamide gel electrophoresis to distinguish allele G and C of the IL-6 gene, allowing the detection of the polymorphism and determination of genotypes. RESULTS: There was a significant difference in the genotype (χ(2) = 12.9, p = 0.001) and allele (χ(2) = 6.62, p = 0.01) distribution between TR patients and control subjects. In a subgroup analysis, there was no significant difference in genotypes and allele frequencies regarding TR recurrence. CONCLUSIONS: This study suggests that the genotypes related with a lower production of IL-6 may be associated with the occurrence of TR.
Assuntos
Coriorretinite/genética , DNA/genética , Interleucina-6/genética , Polimorfismo Genético , Toxoplasmose Ocular/genética , Adulto , Alelos , Animais , Coriorretinite/metabolismo , Coriorretinite/parasitologia , Eletroforese em Gel de Poliacrilamida , Feminino , Seguimentos , Frequência do Gene , Genótipo , Humanos , Interleucina-6/metabolismo , Masculino , Reação em Cadeia da Polimerase , Toxoplasmose Ocular/metabolismo , Toxoplasmose Ocular/parasitologiaRESUMO
This study aimed to investigate the serum levels of the cytokine TNF-α and its soluble receptors (sTNFR1 and sTNFR2) in patients with toxoplasmosis retinochoroidits (TR) and controls. 37 patients with TR and 30 subjects with positive serology for toxoplasmosis but without history and signs of uveitis were included in this study. Serum concentrations of TNF-α, sTNFR1, and sTNFR2 were determined by ELISA. Serum concentrations of TNF-α and sTNFR1 were similar in controls (mean ± SD median values; 56.57 ± 141.96 and 504.37 ± 163.87, respectively) and TR patients (mean ± SD values, 121.62 ± 217.56 and 511.15 ± 189.30, respectively). Serum concentrations of sTNFR2 were higher in the uveitis group when compared to the control group (respectively, mean ± SD values, 1734.84 ± 379.32 and 1442.75 ± 309.47; p=0.002). There was no association between the serum levels of the molecules and the time of first symptoms, severity of vitreous haze, size or localization of active lesions, levels of visual acuity, and presence of vasculitis. These results suggest that TR is associated with changes in the circulating levels of inflammatory biomarkers, but they are not correlated with local/ocular signs.
Assuntos
Adulto , Feminino , Humanos , Masculino , Coriorretinite/sangue , Receptores Tipo I de Fatores de Necrose Tumoral/sangue , Receptores Tipo II do Fator de Necrose Tumoral/sangue , Toxoplasmose Ocular/sangue , Fator de Necrose Tumoral alfa/sangue , Biomarcadores/sangue , Estudos de Casos e Controles , Coriorretinite/parasitologiaRESUMO
This study aimed to investigate the serum levels of the cytokine TNF-α and its soluble receptors (sTNFR1 and sTNFR2) in patients with toxoplasmosis retinochoroiditis (TR) and controls. 37 patients with TR and 30 subjects with positive serology for toxoplasmosis but without history and signs of uveitis were included in this study. Serum concentrations of TNF-α, sTNFR1, and sTNFR2 were determined by ELISA. Serum concentrations of TNF-α and sTNFR1 were similar in controls (mean ± SD median values; 56.57±141.96 and 504.37±163.87, respectively) and TR patients (mean ± SD values, 121.62±217.56 and 511.15±189.30, respectively). Serum concentrations of sTNFR2 were higher in the uveitis group when compared to the control group (respectively, mean ± SD values, 1734.84±379.32 and 1442.75±309.47; p=0.002). There was no association between the serum levels of the molecules and the time of first symptoms, severity of vitreous haze, size or localization of active lesions, levels of visual acuity, and presence of vasculitis. These results suggest that TR is associated with changes in the circulating levels of inflammatory biomarkers, but they are not correlated with local/ocular signs.
Assuntos
Coriorretinite/sangue , Receptores Tipo II do Fator de Necrose Tumoral/sangue , Receptores Tipo I de Fatores de Necrose Tumoral/sangue , Toxoplasmose Ocular/sangue , Fator de Necrose Tumoral alfa/sangue , Adulto , Biomarcadores/sangue , Estudos de Casos e Controles , Coriorretinite/parasitologia , Feminino , Humanos , MasculinoRESUMO
Os autores relatam um caso de vasculite retiniana aguda bilateral em uma paciente de 31 anos de idade, que apresentou, em um dos olhos acometidos, um aspecto fundoscópico semelhante ao quadro descrito na literatura como "periflebite retiniana aguda congelada" ou "angelite de ramos congelados".
Assuntos
Humanos , Feminino , Adulto , Retinite por Citomegalovirus/complicações , Retinite por Citomegalovirus/diagnóstico , Retinite por Citomegalovirus/terapia , Doenças Retinianas/complicações , Doenças Retinianas/etiologia , Doenças Retinianas/terapia , AngiofluoresceinografiaRESUMO
Foram examinados 997 pacientes hansenianos de controle ambulatorial, sendo que 528 (53%) eram da forma Virchowiana, 199 (20%) da forma Dimorfa, 167 (16,8%) da forma Tuberculoide e 103 (10,3%) da forma Indeterminada. 314 pacientes (31,5%) apresentavam alteraçöes do bulbo ocular, e essas manifestaçöes foram mais frequentes na forma Virchowiana e aumentaram de acordo com a idade do paciente e duraçäo da doença. Lesöes oculares severas foram achados raros e essa baixa incidência foi atribuída, em parte, ao tratamento sistêmico precoce
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Cegueira/prevenção & controle , Olho/lesões , Hanseníase/etiologia , BrasilRESUMO
Os AAs realizaram Punçäo de Câmara Anterior em uma paciente portadora de Hanseníase, com quadro de iridociclite bilateral. A Punçäo foi realizada sob anestesia tópica, em regime ambulatorial, e o humor aquoso foi submetido à coloraçäo pelo método de Ziehl-Nielsen, tendo sido isolado o M. Leprae na Câmara Anterior. O trabaho mostra que o M.Leprae é uma das causas de uveítes na Hanseníase, merecendo ser procurado em casos de uveítes em pacientes portadores de Hanseníase