RESUMO
An 8-month-old female Main Coon with a history of recurrent behavioral changes and anorexia was presented with sternal recumbency and depression. Within 5 days, the cat progressively worsened with symptoms of stupor and coma and was euthanized. At post-mortem examination, a solid, grayish infratentorial mass located in the midline rostrally to the cerebellum, was observed. Histologically, highly cellular clusters of small-to-medium undifferentiated cells were intermingled with paucicellular areas with fibrillary eosinophilic (neuropil-like) appearance. Numerous multilayered (ependymoblastic) true rosettes were present. The mitotic activity was frequent (up to 15 mitoses/HPF), involving both undifferentiated cells and rosettes. By immunohistochemistry (IHC), tumor cells were diffusely positive for vimentin, variably for synaptophysin, S-100, and NSE, and focally for NeuN; they were negative for GFAP and CK AE1/AE3. The histological and IHC aspects were consistent with an Embryonal Tumor with Abundant Neuropil and True Rosettes (ETANTR). Embryonal neoplasms of the central nervous system (CNS) are characterized by primitive undifferentiated cells, able to develop toward neuronal, glial, ependymal, and mesenchymal lines. Although extremely rare, juvenile embryonal tumors should be considered in the differentials of CNS disorders in young cats.
RESUMO
BACKGROUND: Leukoreduction is a routine procedure in human transfusion medicine but is uncommon in veterinary. OBJECTIVES: To evaluate the effect of leukoreduction on the quality of canine whole blood (WB) and blood products during storage. ANIMALS: Ten canine blood donors. METHODS: This is a case series study. An amount of 450 mL of blood was collected from each dog. Five WB and 5 packed red blood cells (pRBC) bags were divided into 2 units each: leukoreduced (LR) and non-leukoreduced (nLR). RBC count, erythrocytes' mean osmotic fragility (MOF), 2,3-diphosphoglycerate (2,3-DPG), adenosine triphosphate (ATP), percentage of hemolysis, potassium (K), lactate, glucose, and cytokines were measured weekly from day of donation (T0) to day 35 (T35); pH, coagulation times, and clotting factors were evaluated at T0 and T35 from WB and in fresh frozen plasma after 1 year of storage. RESULTS: Leukoreduction showed positive effects on lactate (T35: LR WB 14.42 mmol/L SD 2.71, nLR WB 22.42 mmol/L SD 1.86, LR pRBC 20.88 mmol/L SD 2.65, nLR pRBC 36.81 mmol/L SD 2.34; P < .0001), pH (T35: LR WB 6.88 SD 0.16, nLR WB 6.69 SD 0.20, P = .02; LR pRBC 6.57 SD 0.23, nLR pRBC 6.22 SD 0.11; P < .001), and K (LR pRBC 4.08 mmol/L SD 0.88, nLR pRBC 5.48 mmol/L SD 0.90; P < .001). Increasing values of IL8 were observed in nLR units during storage (T0: 4167 ± 11 888 pg/mL; T35: 6367 ± 11 612 pg/mL). CONCLUSION AND CLINICAL IMPORTANCE: LR blood units are recommended to critically ill dogs with marked inflammatory conditions.
Assuntos
Preservação de Sangue , Doenças do Cão , Trifosfato de Adenosina , Animais , Preservação de Sangue/veterinária , Citocinas , Cães , Eritrócitos , HemóliseRESUMO
The heterogeneous morphologic features of canine plasmacytomas (PCTs) can make their differentiation from other round cell tumors challenging. Immunohistochemistry (IHC) for lambda (λ) and kappa (к) immunoglobulin (Ig) light chains is often equivocal because of high background staining. The chromogenic in situ hybridization (CISH) technique for light chains has shown higher sensitivity compared to IHC in human plasma cell tumors. Therefore, we aimed to validate automated CISH for light chains in canine tissues and to evaluate its diagnostic potential in canine PCTs, in conjunction with routinely used IHC markers. CISH for light chains demonstrated a clear signal in plasma cell populations of canine control tissues (lymph nodes, lymphoplasmacytic inflammation) showing a polyclonal pattern with a prevalence of λ-producing cells. CISH detected monotypic light chain expression in 33 of 53 (62%) PCTs, 31 expressing λ and 2 expressing к. CISH was more sensitive than IHC for λ light chain (58% vs. 47%, respectively) and more easily interpretable given the absence of confounding background staining. The absence of CISH staining for both λ and к in a considerable subset of tumors may be the result of lower light chain production by neoplastic cells. Multiple myeloma oncogene 1 (MUM1) was expressed by all but 2 PCTs (96%), which showed λ expression by CISH and IHC. The identification of poorly differentiated canine PCTs requires the assessment of a panel of IHC markers, with the potential support of CISH for Ig light chains.
Assuntos
Doenças do Cão/diagnóstico , Cadeias kappa de Imunoglobulina/isolamento & purificação , Cadeias lambda de Imunoglobulina/isolamento & purificação , Hibridização In Situ/veterinária , Plasmocitoma/veterinária , Animais , Cães , Feminino , Hibridização In Situ/métodos , Masculino , Plasmocitoma/diagnósticoRESUMO
In the present study European sea bass (Dicentrarchus labrax) subjected to two different diets (organic vs. conventional) were evaluated in terms of growing performances, oxidative stress, and contaminant markers. Growing performances were evaluated using biometric measures and condition factor (K), whereas insulin-like growth factor (IGF-I and IGF-II) levels were assessed trough Real-Time PCR analysis. For oxidative stress, immunohistochemical staining for 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 4-hydroxy-2-nonenal (HNE) was performed, whereas total glutathione (GSH) in blood serum was determined by an enzymatic method adapted. Cytochrome P4501A (CYP1A) and melanomacrophage centers (MMCs) were evaluated as contaminant markers trough immunohistochemical and histochemical approaches, respectively. The growing performances showed a positive trend in both groups but a greater productivity in conventional fed fish compared to the organic ones. A significant higher expression of MMCs was observed in organic vs. conventional diet fed fish. Fillet analysis showed a higher MUFA content and a lower PUFAs n-6 content in organically fed sea bass indicating that diets with a content in fatty acids closer to that of wild fish will definitely affect the fatty acid profile of the fish flesh. On the other hand, the diet composition did not seem to affect neither the oxidative stress parameters (GSH, 8-OHdG, HNE) nor the CYP1A expression.
RESUMO
CD30 is a transmembrane glycoprotein of the tumor necrosis factor receptor superfamily included in the diagnostic algorithm of human cutaneous, anaplastic large cell and Hodgkin lymphomas and represents an optimal therapeutic target for CD30+ tumors. Similar diagnostic and therapeutic approaches are largely missing for feline lymphomas. Cross-reactivity of the antihuman CD30 receptor clone Ber-H2 was investigated in feline lymphomas. Comparative analysis of feline and human CD30 identified 61% identity of the amino acid sequence, with 100% identity of the main sequence of the epitope targeted by the antibody (RKQCEPDYYL). CD30 expression in normal feline tissues was restricted to rare lymphoid cells in perifollicular and interfollicular lymph node areas and in the thymic medulla. In feline lymphoma, CD30 was expressed in 4 of 33 (13%) T-cell lymphomas, 3 of 22 (14%) B-cell lymphomas, and 5 of 7 (71%) mixed-cell lymphomas, showing diffuse (1/5) or multifocal (4/5) positivity restricted to neoplastic multinucleated lymphoid cells and binucleated cells consistent with Reed-Sternberg-like cells. Based on the human classification system, cell morphology, expression of multiple markers (mixed cell components), and CD30 positivity, these cases were considered most consistent with classical Hodgkin-like lymphoma (HLL). The other 2 mixed-cell lymphomas were CD30 negative and thus most consistent with either T-cell-rich large B-cell lymphoma (TCRLBCL) or nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL). These findings provide multiple data supporting the cross-reactivity of the Ber-H2 anti-CD30 clone in feline tissues and give evidence of the usefulness of CD30 in the diagnostic evaluation of feline lymphoma.
Assuntos
Anticorpos/imunologia , Doenças do Gato/diagnóstico , Epitopos/imunologia , Antígeno Ki-1/metabolismo , Linfoma/veterinária , Animais , Doenças do Gato/classificação , Doenças do Gato/patologia , Gatos , Reações Cruzadas , Feminino , Linfonodos/patologia , Linfócitos/patologia , Linfoma/classificação , Linfoma/diagnóstico , Linfoma/patologia , MasculinoRESUMO
The genus Pneumocystis comprises potential pathogens that reside normally in the lungs of a wide range of mammals. Although they generally behave as transient or permanent commensals, they can occasionally cause life-threatening pneumonia (Pneumocystis pneumonia; PCP) in immunosuppressed individuals. Several decades ago, the presence of Pneumocystis morphotypes (trophic forms and cysts) was described in the lungs of normal cats and cats with experimentally induced symptomatic PCP (after immunosuppression by corticosteroids); yet to date spontaneous or drug-induced PCP has not been described in the clinical feline literature, despite immunosuppression of cats by long-standing retrovirus infections or after kidney transplantation. In this study, we describe the presence of Pneumocystis DNA in the lungs of normal cats (that died of various unrelated causes; n = 84) using polymerase chain reactions (PCRs) targeting the mitochondrial small and large subunit ribosomal RNA gene (mtSSU rRNA and mtLSU rRNA). The presence of Pneumocystis DNA was confirmed by sequencing in 24/84 (29%) cats, with evidence of two different sequence types (or lineages). Phylogenetically, lineage1 (L1; 19 cats) and lineage 2 (L2; 5 cats) formed separate clades, clustering with Pneumocystis from domestic pigs (L1) and carnivores (L2), respectively. Results of the present study support the notion that cats can be colonized or subclinically infected by Pneumocystis, without histological evidence of damage to the pulmonary parenchyma referable to pneumocystosis. Pneumocystis seems most likely an innocuous pathogen of cats' lungs, but its possible role in the exacerbation of chronic pulmonary disorders or viral/bacterial coinfections should be considered further in a clinical setting.
Assuntos
Doenças do Gato/diagnóstico , DNA Fúngico/isolamento & purificação , Pulmão/microbiologia , Pneumocystis/isolamento & purificação , Pneumonia por Pneumocystis/veterinária , Animais , Doenças do Gato/microbiologia , Gatos , Feminino , Masculino , Filogenia , Pneumocystis/genética , Pneumonia por Pneumocystis/diagnóstico , RNA Mitocondrial/isolamento & purificação , RNA Ribossômico/isolamento & purificaçãoRESUMO
BACKGROUND: Optimal procedure for storage of feline blood is needed. Open-collection systems have been employed in feline medicine, thus limiting the possibility for storage. OBJECTIVES: To evaluate indicators of quality of feline blood stored for 35 days at +4°C in a closed-collection system specifically designed for cats. ANIMALS: Eight healthy adult European domestic shorthair cats with a weight of 5-6.8 kg. METHODS: This is a case series study. A bacteriological test, CBC, blood smear, pH, osmotic fragility, 2,3-diphosphoglycerate (2,3-DPG), and adenosine triphosphate (ATP) measurement were performed weekly on whole blood (WB) units from day 1 to day 35 after donation. The hemolysis index, lactate and potassium concentrations, prothrombin time (PT), activated partial thromboplastin time (aPTT), and fibrinogen were measured on plasma aliquots. RESULTS: One out of eight blood units (BUs) had bacterial growth (Serratia marcescens) at day 35. No significant differences were found regarding CBC, morphology, pH, and osmotic fragility. Despite high inter-individual variability and low starting levels, significant decreases in the mean concentrations of 2,3-DPG (T0 1.99 mmol/g Hb, SD 0.52, T35 1.25 mmol/g Hb, SD 1.43; P = .003) and ATP (T0 1.45 mmol/g Hb, SD 0.71, T35 0.62 mmol/g Hb, SD 0.51; P < .001) were detected during the study, as opposed to an increase in hemolysis (T0 0.11 mmol/L, SD 0.07, T35 0.84 mmol/L, SD 0.19; P < .001), lactate (T0 3.30 mmol/L, SD 0.86, T35 13.36 mmol/L, SD 2.90; P < .001), and potassium (T0 3.10 mmol/L, SD 0.21, T35 4.12 mmol/L, SD 0.35; P < .001) concentrations. CONCLUSIONS AND CLINICAL IMPORTANCE: The commercial BU kit is appropriate for blood collection and conservation of WB in cats. The maintenance of WB quality indicators during storage is essential for future improvements of feline transfusion medicine.
Assuntos
Preservação de Sangue/veterinária , Coleta de Amostras Sanguíneas/veterinária , Gatos/sangue , 2,3-Difosfoglicerato/sangue , Trifosfato de Adenosina/sangue , Animais , Contagem de Células Sanguíneas/veterinária , Preservação de Sangue/métodos , Citratos , Fibrinogênio/análise , Glucose , Hemólise , Concentração de Íons de Hidrogênio , Técnicas In Vitro , Ácido Láctico/sangue , Fragilidade Osmótica , Tempo de Tromboplastina Parcial/veterinária , Potássio/sangue , Tempo de Protrombina/veterináriaRESUMO
Although mammary gland tumors (MT) are the most-common type of tumor in intact female dogs, there is little information about their incidence in dog population. Data on MT in female dogs was retrieved from the Animal Tumor registry of dogs and cats of Venice and Vicenza provinces during 2005-2013 and was analyzed to visualize crude incidence rates by breed and across age categories. Overall, 2744 mammary tumors were reported accounting for 54% of all tumors in female dogs. The annual incidence rate (IR) was 250 cases per 100,000 dogs. The most frequent malignant tumors were complex carcinomas, consisting of both epithelial and myoepithelial tissues (IR=71.89), and simple carcinomas (IR=62.59). The MT incidence rate increased through the study period; particularly in the last 4 years, and malignant neoplasms occurred more frequently (70%) than the benign counterparts (30%). Seventy-four percent of tumors were diagnosed in intact females, and the mean age at diagnosis was significantly higher for spayed dogs than for intact ones. MT were less frequent in dogs younger than 6 years and increased up to approximately 60% for ages between 8 and 13 years. The purebred dogs had a higher probability to have a malignant neoplasm than mixed-breed dogs, particularly in dogs younger than 7 years, and the Samoyed, Dobermann, Schnauzer and Yorkshire Terrier breeds were more inclined to develop malignant MT. The incidence of MT in dogs is increasing, and IRs are comparable to that in women. The epidemiological similarities between dogs and women support the validity of canine MT as a model for human breast cancer.
Assuntos
Doenças do Cão/epidemiologia , Neoplasias Mamárias Animais/epidemiologia , Fatores Etários , Animais , Neoplasias da Mama/epidemiologia , Cães , Feminino , Humanos , Incidência , Fatores de Risco , Especificidade da EspécieRESUMO
Formalin-fixed, paraffin-embedded (FFPE) tissues represent a unique source of archived biological material, but obtaining suitable DNA and RNA for retrospective "-omic" investigations is still challenging. In the current study, canine tumor FFPE blocks were used to 1) compare common commercial DNA and RNA extraction kits; 2) compare target gene expression measured in FFPE blocks and biopsies stored in a commercial storage reagent; 3) assess the impact of fixation time; and 4) perform biomolecular investigations on archival samples chosen according to formalin fixation times. Nucleic acids yield and quality were determined by spectrophotometer and capillary electrophoresis, respectively. Quantitative real-time polymerase chain reaction assays for the following genes: BCL-2-associated X protein, B-cell lymphoma extra large, antigen identified by monoclonal antibody Ki-67, proto-oncogene c-KIT (c-kit). Two internal control genes (Golgin A1 and canine transmembrane BAX inhibitor motif containing 4), together with direct sequencing of c-kit exons 8, 9, 11, and 17, were used as end points. Differences in DNA/RNA yield and purity were noticed among the commercial kits. Nucleic acids (particularly RNA) extracted from paraffin blocks were degraded, even at lower fixation times. Compared to samples held in the commercial storage reagent, archived tissues showed a poorer amplification. Therefore, a gold standard protocol for DNA/RNA isolation from canine tumor FFPE blocks for molecular investigations is still troublesome. More standardized storage conditions, including time between sample acquisition and fixation, fixation time, and sample thickness, are needed to guarantee the preservation of nucleic acids and, then, their possible use in retrospective transcriptomic analysis.
Assuntos
DNA/isolamento & purificação , Doenças do Cão/patologia , Mastocitose/patologia , Inclusão em Parafina/veterinária , RNA/isolamento & purificação , Fixação de Tecidos/veterinária , Animais , Doenças do Cão/genética , Cães , Imuno-Histoquímica/veterinária , Antígeno Ki-67/genética , Mastocitose/genética , Inclusão em Parafina/métodos , Proteínas Proto-Oncogênicas c-kit/genética , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Fixação de Tecidos/métodos , Proteína X Associada a bcl-2/genéticaRESUMO
Olfactory communication is an important aspect of the biology of ground squirrels; accordingly, some of their integumentary glands are associated with scent-marking behavior. Although reports of neoplasms in ground squirrels are limited, the literature on tumors in this family of rodents is extensive, with hepatocellular carcinomas in woodchucks and fibromas in squirrels being the 2 most common neoplasms. Apocrine gland tumors occur frequently in domestic animals such as cats and dogs but to our knowledge have not previously been reported in squirrels. Here we describe 2 cases of adenocarcinoma of the dorsal glands in privately owned European ground squirrels (Spermophilus citellus). The skin nodules were characterized histologically by proliferation of epithelial cells, which were arranged in a tubuloacinar pattern with neoplastic emboli within the blood vessels. Adenocarcinoma of the dorsal glands was diagnosed in light of the anatomic localization, immunohistochemistry results, and histochemistry findings.
Assuntos
Adenocarcinoma/veterinária , Sciuridae , Neoplasias das Glândulas Sudoríparas/veterinária , Adenocarcinoma/patologia , Adenocarcinoma/cirurgia , Animais , Feminino , Imuno-Histoquímica/veterinária , Neoplasias das Glândulas Sudoríparas/patologia , Neoplasias das Glândulas Sudoríparas/cirurgia , Resultado do TratamentoRESUMO
Cutaneous mast cell tumors (MCTs) are among the most common neoplasms in dogs and show a highly variable biologic behavior. Histological grading, cell proliferation markers, and KIT immunohistochemistry are typically used to predict post-surgical prognosis. In the present study, c-KIT messenger RNA (mRNA) expression was measured in canine MCTs and its relationship with tumor grade, immunohistochemical staining pattern, post-surgical prognosis, and mutations was investigated. A significant increase of c-KIT mRNA was observed in MCTs versus healthy skin and surgical margins. Mutations were observed in 8.3% of cases. The KIT staining pattern was investigated for both grading systems. In particular, staining pattern III was associated with grade II (G2) and G3 MCTs, while staining patterns I and II were associated with G1 and G2 MCTs. Considering the 2-tier histological grading, the high grade was mainly associated with pattern III (71%) while the low grade was associated with patterns II (70%) and I (28%). A weak association between the KIT staining pattern and outcome was also observed. The results obtained suggest that c-KIT mRNA is overexpressed in canine MCT, although the fold variations were not associated with the protein localization or complementary DNA mutations. These observations suggested that the 3 events were independent. The histological grading and the KIT staining pattern have prognostic value as previously published. Staining pattern I could be especially helpful in predicting a good prognosis of G2 MCTs. Sequence mutations were not necessarily suggestive of a worse prognosis, but might be useful in choosing a chemotherapy protocol.
Assuntos
Doenças do Cão/diagnóstico , Mastocitose Cutânea/veterinária , Proteínas Proto-Oncogênicas c-kit/genética , Animais , Doenças do Cão/genética , Doenças do Cão/patologia , Doenças do Cão/cirurgia , Cães , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Masculino , Mastocitose Cutânea/diagnóstico , Mastocitose Cutânea/genética , Mastocitose Cutânea/patologia , Mastocitose Cutânea/cirurgia , Mutação/genética , Técnicas de Amplificação de Ácido Nucleico/veterinária , Prognóstico , RNA Mensageiro/genética , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Pele/patologiaRESUMO
A free-living European red deer calf (Cervus elaphus) was euthanized due to bilateral microphthalmia. Lens was missing, replaced by proliferating squamous epithelial cells; hyperplastic squamous cells, sebaceous and mucinous glands were observed within the cornea with the characteristics of inclusion cyst. Findings were consistent with congenital microphthalmia/aphakia, with multiple eye abnormalities.
Assuntos
Anormalidades Múltiplas/veterinária , Afacia/veterinária , Cervos/anormalidades , Microftalmia/veterinária , Anormalidades Múltiplas/patologia , Animais , Afacia/patologia , Evolução Fatal , Histocitoquímica/veterinária , Microftalmia/patologiaRESUMO
This report presents peculiar radiographic and computed tomographic features of a lung mass in a dog, later diagnosed by histology as exogenous lipoid pneumonia. Although lipoid pneumonia is a well-known pathological entity in pets, this is the first imaging description of a single infiltrative lesion mimicking a neoplasm.
Assuntos
Doenças do Cão/diagnóstico por imagem , Pneumonia Lipoide/veterinária , Animais , Diagnóstico Diferencial , Doenças do Cão/patologia , Cães , Neoplasias Pulmonares/diagnóstico por imagem , Neoplasias Pulmonares/patologia , Neoplasias Pulmonares/veterinária , Masculino , Pneumonia Lipoide/diagnóstico por imagem , Pneumonia Lipoide/patologia , RadiografiaRESUMO
A 9-year-old, neutered male cat was presented for a subcutaneous mass on the neck. After surgical removal of the mass, a pet identification microchip was found within the tumour. Histological examination of the mass revealed typical features of the feline postinjection sarcoma. The cat had never received injections at the tumour site; all routine vaccinations were administered in the hindlimbs. Few cases of sarcomas developing at the site of microchip application have been reported in animals, although the contributory role of vaccine administrations has not been ruled out. This is the first report of a microchip-associated fibrosarcoma in a cat. Adherence to American Association of Feline Practitioners vaccination guidelines, avoiding the interscapular area, enabled confirmation of the definitive aetiology of the neoplasia.
Assuntos
Sistemas de Identificação Animal/veterinária , Doenças do Gato/etiologia , Fibrossarcoma/veterinária , Neoplasias de Cabeça e Pescoço/veterinária , Próteses e Implantes/veterinária , Sistemas de Identificação Animal/instrumentação , Animais , Doenças do Gato/diagnóstico , Gatos , Fibrossarcoma/diagnóstico , Fibrossarcoma/etiologia , Neoplasias de Cabeça e Pescoço/diagnóstico , Neoplasias de Cabeça e Pescoço/etiologia , Masculino , Próteses e Implantes/efeitos adversosAssuntos
Doenças do Gato/patologia , Fusarium/isolamento & purificação , Granuloma/veterinária , Micoses/veterinária , Rinite/veterinária , Sinusite/veterinária , Animais , Antifúngicos/uso terapêutico , Doenças do Gato/microbiologia , Doenças do Gato/terapia , Gatos , Feminino , Granuloma/microbiologia , Granuloma/patologia , Granuloma/terapia , Itraconazol/uso terapêutico , Micoses/microbiologia , Micoses/patologia , Micoses/terapia , Necrose/patologia , Necrose/veterinária , Rinite/microbiologia , Rinite/patologia , Rinite/terapia , Sinusite/microbiologia , Sinusite/terapiaRESUMO
Histologic and immunohistochemical features of a malignant mesothelioma of the testicular tunica vaginalis in a 9-year-old male mixed-breed dog are reported. The dog had a large scrotal swelling determined by ultrasonography to be a heterogeneous irregular mass characterized by a mixed echogenic pattern and located in the left vaginal canal of the testis. Histologically, the neoplasm was nodular, was poorly demarcated, and consisted of pleomorphic cells that were associated with a significant desmoplastic reaction. By immunohistochemistry, neoplastic cells stained positively for cytokeratin and vimentin and were immunoreactive with an antimesothelial cell monoclonal antibody. Malignant mesotheliomas of tunica vaginalis testis are rare in mammalian species, including dogs.
Assuntos
Doenças do Cão/patologia , Mesotelioma/veterinária , Neoplasias Testiculares/veterinária , Animais , Doenças do Cão/metabolismo , Doenças do Cão/cirurgia , Cães , Evolução Fatal , Imuno-Histoquímica/veterinária , Masculino , Mesotelioma/metabolismo , Mesotelioma/patologia , Mesotelioma/cirurgia , Neoplasias Testiculares/metabolismo , Neoplasias Testiculares/patologia , Neoplasias Testiculares/cirurgiaRESUMO
BACKGROUND: Cancer is a major cause of death in domestic animals. Furthermore, many forms of pet neoplasm resemble that of their human counterparts in biologic behaviour, pathologic expression, and recognised risk factors.In April 2005, a pilot project was activated so as to establish a dog and cat tumour registry living in the Venice and Vicenza provinces (Veneto Region, north-eastern Italy), with the aim of estimating the incidence of spontaneous tumours. RESULTS: Through a telephone survey, the estimates of canine and feline populations of the catchment area turned out to be of 296,318 (CI +/- 30,201) and 214,683 (CI +/- 21,755) subjects, respectively. During the first three years, overall 2,509 canine and 494 feline cases of neoplasia were diagnosed. In dogs, the estimated annual incidence rate (IR) per 100,000 dogs for all tumours was 282 in all the catchment area, whereas in cats the IR was much lower (IR = 77). Malignant and benign tumours were equally distributed in male and female dogs, whereas cats had a 4.6-fold higher incidence of malignant tumours than benign. In both dogs and cats, purebreds had an almost 2-fold higher incidence of malignant tumours than mixed breeds. Tumour incidence increased with age in both dog and cat populations. CONCLUSION: This study has provided estimates of incidence of spontaneous neoplasm in companion animals. Further attempts will be made to increase the accuracy in the population size assessment and to ascertain the real gap with the official regional canine demographic registry. Veterinary practitioners may also benefit from the tumour registry insofar they may obtain data for specific breeds, age groups or geographical areas.
Assuntos
Doenças do Gato/epidemiologia , Doenças do Cão/epidemiologia , Neoplasias/veterinária , Sistema de Registros , Fatores Etários , Animais , Doenças do Gato/patologia , Gatos , Doenças do Cão/patologia , Cães , Feminino , Incidência , Itália , Masculino , Neoplasias/epidemiologia , Neoplasias/patologia , Fatores de Risco , Fatores SexuaisRESUMO
Teratomas are histologically complex tumors arising from germ cells of two or three embryonic layers either in the gonads or occasionally from germ cells that mistakenly migrated to ectopic sites. A case of retroperitoneal teratoma in a regularly slaughtered 4-month-old, male domestic rabbit is reported. A pale reddish, 3 x 4 cm, fibrous mass was detected, loosely connected to the parietal wall in the retroperitoneal region cranial to the kidney. Histologically, the neoplasm contained tissues from ectodermal, endodermal and mesodermal germ layers: nerves and neurons simulating ganglia, cysts lined by squamous epithelium containing keratin and hair, skin adnexa; respiratory glandular epithelium, serous and salivary glands; collagenous connective tissue, muscle fibers, mature cartilage and bone, bone marrow, teeth and fat. To the authors' knowledge this is the first report of a retroperitoneal teratoma in the rabbit.
Assuntos
Coelhos , Neoplasias Retroperitoneais/veterinária , Teratoma/veterinária , Animais , Masculino , Neoplasias Retroperitoneais/patologia , Teratoma/patologiaRESUMO
In August 2007, a 4-year-old, intact, female Domestic Shorthair cat was examined for a mass on the tip of the tail. Histological examination performed after apical caudectomy revealed a neoplasm affecting the distal part of the last coccygeal vertebra. The neoplasm consisted of lobules of physaliferous cells surrounding cartilaginous tissue and a central core of trabecular bone. A diagnosis of chondroid chordoma was made based on histomorphological features and immunohistochemical results. Chondroid chordoma has been previously reported in humans, rats, ferrets, and mink. To the authors' knowledge, chondroid chordoma has not been reported in cats. Neither recurrence nor metastasis was reported 7 months after surgery.