RESUMO
Serological testing for immunoglobulin M (IgM) antibodies to Hepatitis E virus (HEV) is useful for diagnosing acute hepatitis E. Our aim was to evaluate the performance of chemiluminescence immunoassay (HEV IgM; Vircell, Granada, Spain) in comparison with a manual enzyme immunoassay (Anti-HEV virus; Wantai, Beijing, China) for anti-HEV IgM detection. Seventeen HEV-RNA positive and 69 HEV-RNA negative sera were tested by both assays. Virclia sensitivity was 94.1%, whereas that of the enzyme immunosorbent assay (EIA) was 76.5%; specificity was 98.5% and 97.1% for Virclia and EIA, respectively. Differences between both assays were not statistically significant. Virclia HEV IgM showed excellent performance and can be a good alternative to conventional enzyme immunoassays for the detection of IgM against HEV.
Assuntos
Anticorpos Antivirais/sangue , Hepatite E/sangue , Hepatite E/diagnóstico , Hepatite E/imunologia , Técnicas Imunoenzimáticas/métodos , Medições Luminescentes/métodos , Vírus da Hepatite E/imunologia , Humanos , Imunoensaio/métodos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Sensibilidade e Especificidade , Soroconversão , Testes Sorológicos/métodosRESUMO
Trueperella bernardiae es un microorganismo grampositivo facultativo anaerobio que forma parte de la microbiota normal de la piel y de la orofaringe, que recientemente ha sido reasignado l género Trueperella. Este patógeno ha sido descrito en muy pocos casos como causante de infección en los seres humanos, debido a su aspecto corineforme y su presencia en cultivos mixtos, y a las dificultades diagnósticas