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1.
Einstein (Sao Paulo) ; 21: eAO0405, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37970951

RESUMO

OBJECTIVE: To verify the involvement of the endocannabinoid system in the immunomodulatory profile of stem cells from human exfoliated deciduous teeth, in the presence or absence of TNF-α, and agonist and antagonists of CB1 and CB2. METHODS: Stem cells from human exfoliated deciduous teeth were cultured in the presence or absence of an agonist, anandamide, and two antagonists, AM251 and SR144528, of CB1 and CB2 receptors, with or without TNF-α stimulation. For analysis of immunomodulation, surface molecules linked to immunomodulation, namely human leukocyte antigen-DR isotype (HLA-DR), and programmed death ligands 1 (PD-L1) and 2 (PD-L2) were measured using flow cytometry. RESULTS: The inhibition of endocannabinoid receptors together with the proinflammatory effect of TNF-α resulted in increased HLA-DR expression in stem cells from human exfoliated deciduous teeth, as well as, in these cells acquiring an anti-inflammatory profile by enhancing the expression of PD-L1 and PD-L2. CONCLUSION: Stem cells from human exfoliated deciduous teeth respond to the endocannabinoid system and TNF-α by altering key immune response molecules. Inhibition of endocannabinoid receptors and TNF-α led to an increase in HLA-DR, PD-L1, and PD-L2 levels in stem cells from human exfoliated deciduous teeth. This study shows the interaction between mesenchymal stromal cells and the immune and endocannabinoid systems.


Assuntos
Antígeno B7-H1 , Fator de Necrose Tumoral alfa , Humanos , Antígeno B7-H1/metabolismo , Antígeno B7-H1/farmacologia , Diferenciação Celular/fisiologia , Células Cultivadas , Endocanabinoides/farmacologia , Endocanabinoides/metabolismo , Antígenos HLA-DR/metabolismo , Antígenos HLA-DR/farmacologia , Receptores de Canabinoides/metabolismo , Células-Tronco/metabolismo , Dente Decíduo
2.
Einstein (Säo Paulo) ; 21: eAO0405, 2023. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1520851

RESUMO

ABSTRACT Objective To verify the involvement of the endocannabinoid system in the immunomodulatory profile of stem cells from human exfoliated deciduous teeth, in the presence or absence of TNF-α, and agonist and antagonists of CB1 and CB2. Methods Stem cells from human exfoliated deciduous teeth were cultured in the presence or absence of an agonist, anandamide, and two antagonists, AM251 and SR144528, of CB1 and CB2 receptors, with or without TNF-α stimulation. For analysis of immunomodulation, surface molecules linked to immunomodulation, namely human leukocyte antigen-DR isotype (HLA-DR), and programmed death ligands 1 (PD-L1) and 2 (PD-L2) were measured using flow cytometry. Results The inhibition of endocannabinoid receptors together with the proinflammatory effect of TNF-α resulted in increased HLA-DR expression in stem cells from human exfoliated deciduous teeth, as well as, in these cells acquiring an anti-inflammatory profile by enhancing the expression of PD-L1 and PD-L2. Conclusion Stem cells from human exfoliated deciduous teeth respond to the endocannabinoid system and TNF-α by altering key immune response molecules.

3.
Mem. Inst. Oswaldo Cruz ; 106(5): 617-619, Aug. 2011. ilus, graf
Artigo em Inglês | LILACS | ID: lil-597723

RESUMO

The presence of intestinal helminths can down-regulate the immune response required to control mycobacterial infection. BALB/c mice infected with Mycobacterium bovis following an infection with the intestinal helminth Strongyloides venezuelensis showed reduced interleukin-17A production by lung cells and increased bacterial burden. Also, small granulomas and a high accumulation of cells expressing the inhibitory molecule CTLA-4 were observed in the lung. These data suggest that intestinal helminth infection could have a detrimental effect on the control of tuberculosis (TB) and render coinfected individuals more susceptible to the development of TB.


Assuntos
Animais , Camundongos , /biossíntese , Enteropatias Parasitárias/imunologia , Infecções por Mycobacterium/imunologia , Mycobacterium bovis/imunologia , Strongyloides/imunologia , Estrongiloidíase/imunologia , Carga Bacteriana/métodos , Coinfecção , Coinfecção/imunologia , Coinfecção/patologia , Suscetibilidade a Doenças , Enteropatias Parasitárias , Enteropatias Parasitárias/patologia , Pulmão , Pulmão , Camundongos Endogâmicos BALB C , Infecções por Mycobacterium , Infecções por Mycobacterium/patologia , Estrongiloidíase , Estrongiloidíase/patologia
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