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1.
Medicina (Kaunas) ; 59(5)2023 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-37241120

RESUMO

Background and Objectives: The extracellular matrix is important for adipose tissue growth, and numerous interactions between adipocytes and extracellular matrix components occur during adipose tissue development. The main objective of this study was to investigate the interaction and influence of maternal and postnatal diet on adipose tissue remodeling in Sprague Dawley offspring. Materials and Methods: 10 Sprague Dawley females were randomly divided into two groups at nine weeks of age and fed a standard laboratory diet or high-fat diet for six weeks. Then, they were mated, and after birth, their male rat offspring were divided into four subgroups according to diet. After euthanizing the offspring at 22 weeks of age, samples of subcutaneous, perirenal and epididymal adipose tissue were collected. Sections were stained with Mallory's trichrome and analyzed by immunohistochemistry for CD68+ and CD163+ cells. Results: Staining of extracellular components showed higher collagen deposition in the perirenal and epididymal depot of offspring fed a high-fat diet. The number of CD163/CD68+ cells in the perirenal adipose tissue was lower in the CD-HFD group compared with other groups, and in the subcutaneous fat pad when the groups with modified diet were compared with those on non-modified diet. Conclusion: Morphological changes in adipose tissue, increased collagen deposition, and changes in macrophage polarization may be related to intergenerational changes in diet.


Assuntos
Tecido Adiposo Branco , Dieta Hiperlipídica , Animais , Feminino , Masculino , Ratos , Colágeno , Dieta Hiperlipídica/efeitos adversos , Macrófagos , Ratos Sprague-Dawley
2.
Medicina (Kaunas) ; 58(7)2022 Jun 26.
Artigo em Inglês | MEDLINE | ID: mdl-35888573

RESUMO

Background and Objectives: A high-fat diet causes inflammation in the organism and many metabolic disorders. Adipose tissue secretes adipokines that affect the function of many organs. The health status of the mother before and during pregnancy affects the health of the offspring. The aim of this study was to determine how the type of maternal diet and the change in the type of diet in the offspring affects the histological characteristics of the ovaries and subcutaneous and perigonadal adipose tissue in female rat offspring. Materials and Methods: Ten female rats were divided into two groups. One group was fed standard laboratory chow, and the other was fed a high-fat diet and mated with a male of the same breed. The offspring of both groups of dams were divided into four subgroups with different feeding protocols. At 22 weeks of age, the offspring were sacrificed. Ovaries and subcutaneous and perigonadal adipose tissue were isolated. In the ovaries, the presence of cystic formations was investigated. Histomorphometric analysis was performed in two types of adipose tissue. Results: The weight of the ovaries of the offspring of mothers fed a high-fat diet was significantly higher than that of the offspring of mothers fed standard laboratory diets. Cystic formations were found in the ovaries of the offspring of mothers fed a high-fat diet. In subcutaneous adipose tissue, the percentage of small-sized adipocytes was significantly higher in the offspring of mothers fed standard laboratory diets. There were no significant differences in adipocyte surface area and adipocyte number between groups. Conclusion: Maternal diet influences the morphology of the ovaries and adipose tissue of the offspring.


Assuntos
Doenças Metabólicas , Ovário , Tecido Adiposo/metabolismo , Animais , Dieta Hiperlipídica/efeitos adversos , Feminino , Masculino , Doenças Metabólicas/metabolismo , Gravidez , Ratos
3.
Int J Surg Case Rep ; 85: 106138, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34256232

RESUMO

INTRODUCTION AND IMPORTANCE: Internal hernias represent the penetration of intestinal loops through congenital or acquired openings within the mesentery or peritoneum. One such hernia is the paraduodenal hernia, which is the most common type of internal hernia (53%) (Yun et al., 2010) [1]. Due to the variable and non-specific clinical presentation, it is difficult to make a correct diagnosis. Preoperative computed tomography of the abdomen facilitates diagnosis and timely surgical intervention, which can be performed openly or laparoscopically (Coakley et al., 2012) [2]. CASE PRESENTATION: In this paper, the case of a 39-year-old patient with left paraduodenal hernia will be presented. He arrived in the emergency department with sudden onset of abdominal pain. The diagnosis was established using computed tomography and an open surgical procedure was successfully performed. DISCUSSION: Clinical presentation of paraduodenal hernia ranges from asymptomatic to manifest. The greatest difficulty regarding management of paraduodenal hernias lies in their diagnosis. Many studies have shown that the best option for diagnosis is computed tomography (CT). Open and laparoscopic techniques are used in treatment of paraduodenal hernias with similar results. CONCLUSION: The case of a 39-year-old male patient with LPDH with non-specific symptomatology was presented. CT scan is the best diagnostic option for this condition. Open surgical approach was used with great success.

4.
J Matern Fetal Neonatal Med ; 29(9): 1491-7, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26075698

RESUMO

OBJECTIVE: Previous studies established that between generations changes in feeding protocol can have significant impact on reproductive physiology. The aim of the study was to determinate effects of mothers' nutrition and nutrition of the offspring on the characteristics of vaginal smear and serum lipid content. METHODS: Ten female rats were randomly divided in two groups; first group fed with food containing high content of saturated fatty acids (HFD) and the second with standard laboratory chow (CD). After coupling and lactation period their offspring were further randomly divided into two subgroups fed HFD or CD forming four study groups: (a) CD-CD, (b) CD-HFD, (c) HFD-CD and (d) HFD-HFD. The dams and offspring at the age of 37 and 18 weeks, respectively, were subjected to biochemical analysis of the blood and cytological analysis of the vaginal smears. Additionally body weight was recorded and body mass index (BMI) was calculated. RESULTS: The HFD-HFD group presented with highest levels of triglycerides and the CD-HFD with the highest levels of cholesterol. Therefore, triglyceride and cholesterol levels were significantly different among the groups (p = 0.001 and p = 0.002, respectively). Vaginal cytological smears analysis showed features of irregular phase interchanges or extended estrous phase in offspring of high-fat fed dams. CONCLUSION: Maternal HFD consumption predisposes offspring to increased risk of developing metabolic abnormalities and estrous disorders.


Assuntos
Dieta Hiperlipídica/efeitos adversos , Ciclo Estral , Lipídeos/sangue , Efeitos Tardios da Exposição Pré-Natal/etiologia , Animais , Índice de Massa Corporal , Peso Corporal , Feminino , Gravidez , Distribuição Aleatória , Ratos Sprague-Dawley , Esfregaço Vaginal
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