[Mixed gel precipitation in the analysis of monoclonal antibodies against determinants of the carcinoembryonic antigen]. / Smeshannaia pretsipitatsiia v gele pri analize monoklonal'nykh antitel k raznym determinantam rakovoémbrional'nogo antitela.

Mixed gel precipitation technique was used in the study of 5 monoclonal antibodies (MA) to carcinoembryonic antigen (CEA). The method is based on specific inclusion of MA into the precipitate formed by the antigen and polyclonal antibodies to it. The test-system for the determination of nonspecific cross-reacting antigen (NCA) has demonstrated that MA 2D10 are directed to the determinant, common for CEA and NCA, whereas MA 3C12 and 2G10 give no reaction with NCA. The specificity of MA 192 and 35 correlated with the previously established one. Mixed gel precipitation technique is recommended for primary screening and study of MA specificity to any precipitating antigen.