RESUMO
Pus discharge containing black granular materials (1-2 mm in diameter) was found in the abdominal skin of a 13-year-old sterilized female cat. Abdominal ultrasonography revealed a large intra-abdominal mass with abundant blood flow beneath the skin lesion. Laparotomy revealed a large mass that adhered to the spleen and left kidney. Similar small lesions were found in the abdominal wall and mesentery. The masses were surgically removed along with the spleen and kidney. Histopathologically, the mass lesions consisted of granulomas with lesional pigmented fungi, and the cat was diagnosed with phaeohyphomycosis. Uisng genetic analysis, the Exophiala dermatitidis was identified as the causative pathogen.
Assuntos
Doenças do Gato , Exophiala , Feoifomicose , Animais , Feoifomicose/veterinária , Feoifomicose/microbiologia , Feoifomicose/diagnóstico , Feoifomicose/patologia , Doenças do Gato/microbiologia , Doenças do Gato/diagnóstico , Doenças do Gato/patologia , Feminino , Gatos , Exophiala/isolamento & purificaçãoRESUMO
This report described the histopathological and immunohistochemical features of cutaneous mast cell tumor (MCT) in six hedgehogs. The hedgehogs presented single cutaneous mass with ulcer and crusting. Histologically, the neoplastic lesions were characterized by the proliferation of well-differentiated mast cells (3 cases), and atypical mast cells (3 cases) with one atypical histiocytic morphology. Immunohistochemically, tumor cells were positive for KIT and mast cell tryptase, and were negative for Iba-1. In well-differentiated MCT, all patients were clinically improved and survived more than 365 days after surgical excision, whereas an atypical histiocytic MCT showed aggressive behavior with re-recurrence, and the animal died 115 days after surgery. These findings suggest that, compatible with other animals, well-differentiated MCT has a better prognosis in hedgehogs.
Assuntos
Ouriços , Mastócitos , Animais , Macrófagos , PeleRESUMO
A retrospective study involving eight African pygmy hedgehogs histopathologically diagnosed with granulosa cell tumors was conducted. The age at onset was 2.2-4.5 years, with a median age of 3.6 years. The most common clinical signs were hematuria and abdominal distension, which were observed in >50% cases. Exploratory laparotomy was performed in all cases, and ovariohysterectomy or excision of the abdominal mass was performed. Patients with only hematuria survived for >250 days after surgery, whereas those with initial ascites showed recurrence of ascites or tumor growth and survived for approximately 130 days after surgery. Intraperitoneal injection of carboplatin was performed in three recurrent cases. In one of these three cases, the tumor mass disappeared. Hence, carboplatin can be considered a potential antineoplastic drug for the treatment of granulosa cell tumors.
Assuntos
Tumor de Células da Granulosa , Neoplasias Ovarianas , Animais , Feminino , Tumor de Células da Granulosa/tratamento farmacológico , Tumor de Células da Granulosa/cirurgia , Tumor de Células da Granulosa/veterinária , Ouriços , Recidiva Local de Neoplasia/veterinária , Neoplasias Ovarianas/tratamento farmacológico , Neoplasias Ovarianas/cirurgia , Neoplasias Ovarianas/veterinária , Estudos RetrospectivosRESUMO
The protective property of propolis across a wide spectrum of diseases has long been realized, yet the anti-tumor efficacy of this bioactive substance from Philippine stingless bees has remained poorly understood. Here, we showed the tumor-suppressing potential of crude ethanolic extract of Philippine stingless bee propolis (EEP) in in vitro models of gastric cancer highlighting the first indication of remarkable subtype specificity towards differentiated-type human gastric cancer cell lines but not the diffuse-type. Mechanistically, this involved the profound modulation of several cell cycle related gene transcripts, which correlated with the prominent cell cycle arrest at the G0/G1 phase. To reinforce our data, a unique differentiated-type gastric cancer model, A4gnt KO mice, together with age-matched 60 week-old C57BL/6 J mice were randomly assigned to treatment groups receiving distilled water or EEP for 30 consecutive days. EEP treatment induced significant regression of gross and histological lesions of gastric pyloric tumors that consistently corresponded with specific transcriptional regulation of cell cycle components. Also, the considerable p21 protein expression coupled with a marked reduction in rapidly dividing BrdU-labeled S-phase cells unequivocally supported our observation. Altogether, these findings support the role of Philippine stingless bee propolis as a promising adjunct treatment option in differentiated-type gastric cancer.
Assuntos
Adenocarcinoma , Neoplasias Experimentais , Própole/farmacologia , Neoplasias Gástricas , Adenocarcinoma/tratamento farmacológico , Adenocarcinoma/metabolismo , Adenocarcinoma/patologia , Animais , Abelhas , Linhagem Celular Tumoral , Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos , Humanos , Camundongos , Camundongos Knockout , Neoplasias Experimentais/tratamento farmacológico , Neoplasias Experimentais/metabolismo , Neoplasias Experimentais/patologia , Fase de Repouso do Ciclo Celular/efeitos dos fármacos , Neoplasias Gástricas/tratamento farmacológico , Neoplasias Gástricas/metabolismo , Neoplasias Gástricas/patologiaRESUMO
Canine anal sac gland carcinoma (ASGC) frequently occurs in the apocrine glands of the canine anal sac and shows aggressive biological behavior. The expression of human epidermal growth factor receptor 2 (HER2) has been reported in various human and canine tumors. HER2 is a promising therapeutic target of these tumors, and HER2-targeted drugs, such as trastuzumab and lapatinib, have improved the outcome of these patients. In this study, HER2 expression in ASGC was evaluated to investigate its potential as a therapeutic target for canine ASGC. HER2 mRNA expression in surgically resected ASGC tissues was significantly higher than that in normal anal sac tissue. To evaluate the expression of HER2 protein, paraffin-embedded ASGC tissues were immunohistochemically evaluated. Strong and broad staining of HER2 was detected in ASGC tissues, while HER2 was weakly to moderately stained in normal anal sac apocrine glands and squamous epithelia. The degree of HER2 expression in ASGC tissues was scored based on its intensity and positivity (score: 0-3+). Scoring of HER2 expression revealed 6 samples (24%) scored 3+, 14 (56%) scored 2+, and 5 (20%) scored 1+, with no samples scoring 0. In all, 80% of canine ASGC tissues were positive for HER2 (scored ≥2+). Furthermore, putative HER2-overexpressed cells in ASGC were detected with trastuzumab by flow cytometry. These preliminary data may lead to further evaluation of the role of HER2 in canine ASGC as a mechanism of malignancy and as a therapeutic target for HER2-targeted therapy.
Assuntos
Neoplasias das Glândulas Anais/metabolismo , Carcinoma/veterinária , Doenças do Cão/metabolismo , Receptor ErbB-2/metabolismo , Neoplasias das Glândulas Anais/genética , Sacos Anais/metabolismo , Animais , Glândulas Apócrinas/metabolismo , Carcinoma/genética , Carcinoma/metabolismo , Doenças do Cão/genética , Cães , Citometria de Fluxo/veterinária , Regulação Neoplásica da Expressão Gênica , Imuno-Histoquímica , RNA Mensageiro , Receptor ErbB-2/genética , Trastuzumab/farmacologiaRESUMO
Human enteropathy-associated T-cell lymphoma (EATL) is classified into 2 distinct subgroups based on their phenotypes (type I and type II). Canine intestinal T-cell lymphoma can be morphologically classified into large and small cell lymphomas (LCL and SCL, respectively). Their association with human EATL or immunohistochemical and biological features has not been well characterized. In this study, the immunohistochemical profiles of 17 cases of LCL and 33 cases of SCL were evaluated with markers used for human EATL classification. Morphologically, LCL was characterized by sheet-like proliferation of large to moderately sized neoplastic lymphocytes, with scant clear cytoplasm and pleomorphic, irregularly shaped nuclei containing distinctive nucleoli and scattered chromatin. In contrast, SCL was characterized by the proliferation of monomorphic small neoplastic lymphocytes, accompanied by infiltration of nonneoplastic plasma cells. Interestingly, 8 cases demonstrated mixed LCL and SCL morphologies. Granular cytoplasmic expression of granzyme B was observed in most LCL and SCL cases. Membranous expression of CD56 was demonstrated in only 2 of 17 LCL and 0 of 33 SCL cases. Coexpression of CD20 by neoplastic T cells was observed in more SCL cases (16/33; 48%) than LCL cases (1/17; 6%). The CD56-positive cells in 2 cases were negative for CD20. Although canine LCL shares common features with human EATL type I, canine SCL cells and human EATL type II differ in their immunophenotype. Canine intestinal T-cell lymphoma had a homogeneous immunophenotype regardless of cell morphology. The findings of this study may indicate large cell transformation of SCL rather than 2 distinct entities.
Assuntos
Doenças do Cão/patologia , Imuno-Histoquímica/veterinária , Linfoma de Células T/veterinária , Animais , Cães , Imuno-Histoquímica/métodos , Linfoma de Células T/patologiaRESUMO
ß-Glucan refers to a heterogeneous group of chemically defined storage polysaccharides containing ß-(1,3)-d-linked glucose polymers with branches connected by either ß-(1,4) or ß-(1,6) glycosidic linkage. To date, an extensive amount of scientific evidence supports their multifunctional biological activities, but their potential involvement in the progression of premalignant lesions remains to be clarified. A4gnt KO mice that lack α1,4-N-acetylglucosamine-capped O-glycans in gastric gland mucin are a unique animal model for gastric cancer because the mutant mice spontaneously develop gastric cancer through hyperplasia-dysplasia-adenocarcinoma sequence. In particular, A4gnt KO mice show gastric dysplasia during 10-20â¯weeks of age. Here we investigated the putative gastro-protective activity of brown seaweed-derived ß-glucan (Laminaran) against development of gastric dysplasia, precancerous lesion for gastric cancer in A4gnt KO mice. The mutant mice at 12â¯weeks of age were randomly assigned into three treatment groups namely, wildtype controlâ¯+â¯distilled water (normal control), A4gnt KO miceâ¯+â¯distilled water (untreated control), and A4gnt KO miceâ¯+â¯100â¯mg/kg Laminaran. After 3â¯weeks, the stomach was removed and examined for morphology and gene expression patterns. In contrast to the untreated control group, administration of Laminaran substantially attenuated gastric dysplasia development and counterbalanced the increased induction in cell proliferation and angiogenesis. Furthermore, Laminaran treatment effectively overcame the A4gnt KO-induced alteration in the gene expression profile of selected cytokines as revealed by real-time PCR analysis. Collectively, our present findings indicate that ß-glucan can potentially restrain the development of gastric dysplasia to mediate their tissue-preserving activity.
Assuntos
Adenocarcinoma/prevenção & controle , Anticarcinógenos/uso terapêutico , Glucanos/uso terapêutico , Phaeophyceae , Alga Marinha , Neoplasias Gástricas/prevenção & controle , Animais , Anticarcinógenos/farmacologia , Citocinas/genética , Feminino , Mucosa Gástrica/efeitos dos fármacos , Mucosa Gástrica/imunologia , Mucosa Gástrica/patologia , Regulação da Expressão Gênica/efeitos dos fármacos , Glucanos/farmacologia , Masculino , Camundongos Knockout , FitoterapiaRESUMO
Four aged retired Chinese native pigs, three females and one male, estimated as over 10-year-old, were subjected to autopsy because of infertility due to aging. Grossly, nodular lesions were found bilaterally in the adrenal medulla of all four pigs. Based on the gross and the histopathological findings, they were diagnosed as either medullary nodular hyperplasia or pheochromocytoma. Immunohistochemically, proliferating cells of all these lesions were immuno-positive for chromogranin-A, indicating adrenal medulla-derived. Ultrastructurally, cytoplasmic neurosecretory granules suggestive of secretion were observed in these proliferating cells. There have been only limited numbers of reports on adrenal medullar proliferative changes including pheochromocytoma in pigs. The present cases will provide a valuable information for the characterization of similar changes in animals and human.
Assuntos
Neoplasias das Glândulas Suprarrenais/veterinária , Medula Suprarrenal/patologia , Feocromocitoma/veterinária , Doenças dos Suínos/diagnóstico , Suínos , Neoplasias das Glândulas Suprarrenais/diagnóstico , Neoplasias das Glândulas Suprarrenais/patologia , Hiperfunção Adrenocortical , Animais , Feminino , Hiperplasia , Masculino , Feocromocitoma/diagnósticoRESUMO
Canine squamous cell carcinoma (SCC) shows highly invasive and locally destructive growth. In animal models and human cancer cases, periostin plays a critical role in the enhancement of cancer growth; however, the mechanism of involvement in canine cancers remains unknown. The aim of this study was to examine the involvement of periostin in the pathophysiology of SCC in dogs. We examined the localization of periostin and periostin-producing cells in 20 SCC and three squamous papilloma specimens. Furthermore, we focused on transforming growth factor (TGF)-ß1, which was assumed to be an inducing factor of periostin, using culture cells. By immunohistochemistry, limited periostin expression in the stroma was observed in all squamous papillomas. In SCC, periostin protein diffusely expressed at the tumor invasion front of cancer growth. In situ hybridization revealed that periostin mRNA was expressed in the stromal fibroblasts in SCC. In vitro analysis determined that canine SCC cells expressed significantly higher levels of TGF-ß1 mRNA compared with canine keratinocytes. In addition, recombinant TGF-ß1 induced secretion of periostin from cultured dermal fibroblasts. These data suggest that periostin produced by stromal fibroblasts may be involved in the pathophysiology of canine SCC. TGF-ß1 derived from SCC cells may stimulate fibroblasts to produce periostin.
Assuntos
Carcinoma de Células Escamosas/veterinária , Moléculas de Adesão Celular/metabolismo , Doenças do Cão/metabolismo , Animais , Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/patologia , Linhagem Celular Tumoral , Doenças do Cão/patologia , Cães , Feminino , Fibroblastos/metabolismo , Hibridização In Situ , Masculino , Fator de Crescimento Transformador beta1/metabolismoRESUMO
A 7-year-old castrated male ferret developed unilateral cervical lymphadenomegaly over a 1-month period. Histological examination revealed proliferation of tumor cells in a diffuse and partially nodular pattern. The tumor cells were predominantly Hodgkin cells and binucleated Reed-Sternberg cells, characterized by abundant, clear, vacuolated cytoplasm, pleomorphic, ovoid nuclei with thick nuclear membranes and distinct nucleoli. Multinucleated cells, resembling lymphocytic and histiocytic (L&H) cells, were also observed. Immunohistochemically, the tumor cells expressed Pax-5, BLA-36 and vimentin. A small population of the tumor cells expressed CD20. This case showed proliferation of Hodgkin/Reed-Sternberg cells in conjunction with L&H cells that were histologically analogous to feline Hodgkin's-like lymphoma. However, Pax-5 and BLA-36 expression along with rare CD20 expression were consistent with classical Hodgkin's lymphoma in humans.
Assuntos
Furões , Doença de Hodgkin/veterinária , Animais , Antígenos CD20/metabolismo , Doença de Hodgkin/diagnóstico , Doença de Hodgkin/patologia , Linfonodos/patologia , Masculino , Fator de Transcrição PAX5/metabolismo , Vimentina/metabolismoRESUMO
Anaplastic large T-cell lymphoma (ALTCL) is rarely reported in domestic animals. Accordingly, the histopathological and immunohistochemical characteristics of ALTCL have not been well established in prairie dogs. The present report documents three cases in which prairie dogs were diagnosed with ALTCL arising in the subcutaneous tissue, oral mucosa or the mesenteric lymph nodes. Of the three cases, one was available for necropsy and the others were biopsy cases. Microscopically, moderate to large, pleomorphic neoplastic lymphocytes with ovoid to polygonal, bizarre-shaped nuclei, abundant cytoplasm and eosinophilic granules were seen in all cases. Immunohistochemical staining revealed membranous or cytoplasmic CD3 expression of the neoplastic lymphocytes. The neoplastic cells often had granzyme B-positive cytoplasmic granules. One of the prairie dogs with nodal ALTCL suffered systemic dissemination of the tumor and died suddenly. In the two biopsy cases, one animal died on the day of the biopsy examination and the other died six weeks after chemotherapy. ALTCL in prairie dogs displays a cytotoxic T cell phenotype and presumably carries a poor prognosis regardless of the anatomical type.
Assuntos
Linfoma Anaplásico de Células Grandes/veterinária , Sciuridae , Animais , Antígenos CD/análise , Feminino , Imuno-Histoquímica/veterinária , Linfoma Anaplásico de Células Grandes/patologia , MasculinoRESUMO
Histiocytic sarcoma is a progressive and fatal malignant neoplasm that mainly occurs in middle- to old-aged dogs. This study describes clinicopathological, histological and immunohistochemical characteristics of intracranial histiocytic sarcomas in 23 dogs. Magnetic resonance imaging and/or computed tomography of the brains revealed that the tumors mainly located in the cerebrum, particularly the frontal lobe. Seizure was a predominant clinical sign in most of the cases. Histologically, the tumor cells were morphologically classified into round/polygonal- and spindle-shaped cell types. There was a significant association between tumor cell types and hemophagocytic activity (P<0.05). However, there was no significant difference in other clinicopathological parameters and mitotic index between the 2 types. Immunohistochemically, tumor cells were strongly positive for HLA-DR, Iba-1 and CD204 in all the 23 cases, for iNOS in 20, for CD163 in 17, for CD208 (DC-LAMP) in 9, for lysozyme in 8 and for S100 in 5 cases. In addition, the Ki67-proliferative index showed range of 0.50-64.33% (Average 26.60 ± 3.81%). These observations suggest that canine primary intracranial histiocytic sarcomas tend to exhibit both dendritic cell and macrophage phenotypes of histiocytic differentiation.
Assuntos
Neoplasias Encefálicas/veterinária , Doenças do Cão/patologia , Sarcoma Histiocítico/veterinária , Animais , Biomarcadores Tumorais/metabolismo , Neoplasias Encefálicas/patologia , Cães , Feminino , Sarcoma Histiocítico/patologia , Imuno-Histoquímica/veterinária , MasculinoRESUMO
A 10-year-old castrated male mixed-breed dog exhibited vomiting, lethargy and anorexia. An abdominal ultrasound examination revealed a focal dilation of the abdominal aorta. The dog died 2 days after examination, and necropsy revealed a ruptured aneurysm at the abdominal aorta between the kidneys. Histological examination revealed severe granulomatous and necrotizing pan-arteritis with fungal organisms. Graphium species was identified through DNA sequence analysis of the PCR product from formalin-fixed paraffin-embedded samples. To our knowledge, this is the first report of aortic aneurysm caused by Graphium species in a dog. Sequence was submitted to the DNA Data Bank of Japan with the accession number LC007972.
Assuntos
Aneurisma Infectado/veterinária , Aneurisma Aórtico/veterinária , Ascomicetos/isolamento & purificação , Doenças do Cão/microbiologia , Aneurisma Infectado/microbiologia , Animais , Aneurisma Aórtico/microbiologia , Aneurisma Aórtico/patologia , Ascomicetos/classificação , Cães , Evolução Fatal , MasculinoRESUMO
Choroid plexus tumor (CPT) is a primary intracranial neoplasm of the choroid plexus epithelium in the central nervous system. In the current World Health Organization classification, CPT is classified into two categories; choroid plexus papilloma (CPP) and carcinoma (CPC). In the present study, we investigated immunohistochemical expressions of N-cadherin, E-cadherin and ß-catenin in 5 canine CPT cases (1 disseminated CPC, 2 CPCs and 2 CPPs). One CPP case was positive for N-cadherin and ß-catenin, but negative for E-cadherin. The disseminated CPC case was positive for E-cadherin and ß-catenin, but negative for N-cadherin. The other cases were positive for the three molecules examined. These results suggest that loss of the N-cadherin expression might associate with the spreading of CPC cells.
Assuntos
Moléculas de Adesão Celular/metabolismo , Neoplasias do Plexo Corióideo/veterinária , Doenças do Cão/metabolismo , Regulação Neoplásica da Expressão Gênica/fisiologia , Animais , Moléculas de Adesão Celular/genética , Neoplasias do Plexo Corióideo/metabolismo , Cães , Feminino , MasculinoRESUMO
Senile plaques (SPs) and cerebral amyloid angiopathy (CAA) consisting of ß-amyloid (Aß) are major features in the brain of Alzheimer's disease (AD) patients and elderly humans and animals. In this study, we report the finding of SPs and CAA in an aged sea lion (30 years), which is the first demonstration of AD-related pathological changes in a marine animal. Histologically, SPs were observed at the cerebral cortex, most frequently at the frontal lobe, with two morphologically different types: the small round type and the large granular type. Only the small round SPs were positive for Congo red staining. The SPs were equally immunoreactive to Aß40 and Aß42 and were mainly composed of Aß with an N-terminal pyroglutamate residue at position 3. Amyloid depositions at vessel walls were noted at the meninges and within the parenchyma. Interestingly, double immunofluorescence staining for Aß40 and Aß42 showed that the two subtypes were deposited segmentally in different parts of the vessel walls. The lesions observed in the sea lion suggest that Aß deposition is widely present in various animal species, including marine mammals; however, the peculiar deposits similar to cotton wool plaques and the specific pattern of CAA are characteristic features of this animal.
Assuntos
Vasos Sanguíneos/patologia , Angiopatia Amiloide Cerebral/veterinária , Lobo Frontal/patologia , Meninges/patologia , Placa Amiloide/veterinária , Leões-Marinhos/metabolismo , Fatores Etários , Peptídeos beta-Amiloides/química , Animais , Vasos Sanguíneos/química , Angiopatia Amiloide Cerebral/diagnóstico , Angiopatia Amiloide Cerebral/metabolismo , Angiopatia Amiloide Cerebral/patologia , Lobo Frontal/irrigação sanguínea , Lobo Frontal/química , Imuno-Histoquímica , Masculino , Meninges/irrigação sanguínea , Meninges/química , Fragmentos de Peptídeos/química , Placa Amiloide/química , Placa Amiloide/diagnóstico , Placa Amiloide/patologiaRESUMO
In the current study, a case of a cardiac ganglioneuroma with systemic metastases in a cat is described. A 12-year-old male neutered Japanese domestic cat was brought to a veterinary hospital for dysorexia, coughing, vomiting, and diarrhea. Ultrasonography revealed a mass adjacent to the right atrium. The animal died of respiratory failure 1 month after the first visit to the hospital. At necropsy, an oval-shaped white mass 1.5 cm in diameter was observed within the right auricle. Diffusely, the right ventricle was infiltrated and thickened by the neoplastic lesion. Histologically, the mass was composed of 3 types of neoplastic cells: spindle cells, large polygonal cells, and small undifferentiated cells. Immunohistochemically, the neoplasia was positive for neuronal markers such as ßIII tubulin, S-100a, and protein gene product 9.5. Ultrastructurally, the large polygonal cells were characterized by abundant cytoplasm that included compressed Golgi cisternae and rough endoplasmic reticula and abundant intermediate filaments. A discontinuous basement membrane surrounded the spindle cells. Metastatic foci were found in the lungs, kidney, pancreas, urinary bladder, and adrenal glands. The morphological, immunohistochemical, and ultrastructural characteristics of the tumor cells were consistent with those of ganglioneuroma. The tumor was presumed to originate from the intramural parasympathetic ganglia in the right atrium.
Assuntos
Doenças do Gato/patologia , Ganglioneuroma/veterinária , Neoplasias Cardíacas/veterinária , Animais , Gatos , Evolução Fatal , Ganglioneuroma/patologia , Ganglioneuroma/ultraestrutura , Neoplasias Cardíacas/patologia , Neoplasias Cardíacas/ultraestrutura , Imuno-Histoquímica/veterinária , Masculino , Microscopia Eletrônica de Transmissão/veterináriaRESUMO
Mutant forms of transthyretin (TTR) cause the most common type of autosomal-dominant hereditary systemic amyloidosis. In addition, wild-type TTR causes senile systemic amyloidosis, a sporadic disease seen in the elderly. Although spontaneous development of TTR amyloidosis had not been reported in animals other than humans, we recently determined that two aged vervet monkeys (Chlorocebus pygerythrus) spontaneously developed systemic TTR amyloidosis. In this study here, we first determined that aged vervet monkeys developed TTR amyloidosis and showed cardiac dysfunction but other primates did not. We also found that vervet monkeys had the TTR Ile122 allele, which is well known as a frequent mutation-causing human TTR amyloidosis. Furthermore, we generated recombinant monkey TTRs and determined that the vervet monkey TTR had lower tetrameric stability and formed more amyloid fibrils than did cynomolgus monkey TTR, which had the Val122 allele. We thus propose that the Ile122 allele has an important role in TTR amyloidosis in the aged vervet monkey and that this monkey can serve as a valid pathological model of the human disease. Finally, from the viewpoint of molecular evolution of TTR in primates, we determined that human TTR mutations causing the leptomeningeal phenotype of TTR amyloidosis tended to occur in amino acid residues that showed no diversity throughout primate evolution. Those findings may be valuable for understanding the genotype-phenotype correlation in this inherited human disease.
Assuntos
Amiloidose Familiar/genética , Chlorocebus aethiops/genética , Modelos Animais de Doenças , Pré-Albumina/genética , Alelos , Sequência de Aminoácidos , Amiloidose Familiar/metabolismo , Amiloidose Familiar/patologia , Animais , Chlorocebus aethiops/sangue , Testes de Função Cardíaca , Humanos , Macaca fascicularis/sangue , Macaca fascicularis/genética , Dados de Sequência Molecular , Mutação , Pré-Albumina/metabolismo , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência , Homologia de Sequência de AminoácidosRESUMO
Senile systemic amyloidosis (SSA) is a rather common disease in elderly people, but it is very rare in animals, including nonhuman primates. Pathological examination of a 26-year-old male savannah monkey (Cercopithecus aethiops) revealed systemic amyloidosis with severe cardiac fibrosis, and tenosynovial degeneration of the elbow and knee joints. The amyloid deposits were observed predominantly in the heart, lung, intestine and tenosynovium, and were positive for transthyretin (TTR) in immunohistochemistry. Immunohistochemical results, together with the distribution of the amyloid deposited lesions and the age of the monkey, were equivalent to those of human SSA. This is the second case of animal SSA with unprecedented TTR amyloid deposited lesions of the tenosynovium resembling human SSA. There may be a genetic factor that makes this species susceptible to SSA, since SSA has been reported in no other mammal besides humans.
Assuntos
Amiloidose/veterinária , Chlorocebus aethiops , Doenças dos Macacos/patologia , Amiloidose/patologia , Animais , Animais de Zoológico , Evolução Fatal , Fibrose/patologia , Fibrose/veterinária , Átrios do Coração/patologia , Ventrículos do Coração/patologia , Humanos , Japão , Masculino , Miocárdio/patologia , Especificidade da EspécieRESUMO
A 5-year-old painted turtle (Chrysemys picta) presented with a 1cm nodular enlargement of the right femorotibial joint. The right leg was amputated, because the nodule continued to grow and motor problems appeared. The cut surface of the nodule was yellowish white, and had a colloidal to chalky gross appearance. Microscopically, a multinodular foreign-body granuloma was observed around the joint capsule. Morphological and histochemical examination showed that the foreign body consisted of calcium pyrophosphate. Histological findings revealed that the articular cartilage was mainly involved in the pathological onset of the disease, and was therefore diagnosed as tophaceous pseudogout. This disease is generic in elderly people, but is rare in animals. Also, there are only three reports in reptiles.