[Analysis of Reproductive Tract Microecological Changes During the Frozen-Thawed Embryo Transfer Cycle and Clinical Pregnancy Outcomes]. / æ¿€ç´ æ›¿ä»£å†»èƒšç§»æ¤å‘¨æœŸç”Ÿæ®–é“å¾®ç”Ÿæ€å˜åŒ–ä¸Žå¦Šå¨ ç»“å±€åˆ†æž.

Objective: This study aims to analyze the relationship between reproductive tract microecological changes, metabolic differences, and pregnancy outcomes at different time points in the frozen-thawed embryo transfer cycle while patients are undergoing hormone replacement therapy, which will be a breakthrough point for improving outcomes. Methods: A total of 20 women undergoing frozen-thawed single blastocyst transfer for the first time at the Reproductive Medicine Center of Fujian Maternal and Child Health Hospital between July 2022 and January 2023 were recruited for this study. Their vaginal and cervical secretions were collected for 16S rRNA sequencing and non-targeted metabolomics analysis on days 2-5 of menstruation, day 7 after estrogen replacement therapy started, the day when progesterone was added, and the day of transplantation. The subjects were divided into different groups according to their clinical pregnancy status and the sequencing results were analyzed using bioinformatics methods. Results: 1) The alpha-diversity index of the vaginal and cervical microbiota was higher on days 2-5 of menstruation (P<0.01), but did not differ significantly on day 7 after oral estrogen replacement therapy started, the day of progesterone administration, and the day of transplantation (P≥0.1). 2) Both the pregnant group and the non-pregnant group showed a variety of microorganisms and metabolites with significant differences in the lower reproductive tract at different time points. 3) Microbial analysis at different time points showed that there were significant differences in vaginal flora, including Peptoniphilus, Enterocloster, Finegoldia, Klebsiella, Anaerobutyricum, Agathobaculum, Sporanaerobacter, Bilophila, Prevotella, and Anaerococcus in the pregnant group (P<0.05). 4) Metabolite analysis at different time points showed that there were significant differences in 3-hydroxybenzoic acid, linatine, (R)-amphetamine, hydroxychloroquine, and L-altarate in the vaginal secretions of the pregnant group (P<0.05), and that there were significant differences in isocitric acid, quassin, citrinin, and 12(R)-HETE in the cervical secretions (P<0.05). 5) Metabolite analysis at different time points showed that, in the non-pregnant group, there were significant differences in linatine, decanoyl-L-carnitine, aspartame, sphingosine, and hydroxychloroquine in the vaginal secretions (P<0.05), and the isocitric acid, quassin, ctrinin, and 12(R)-HETE in the cervical secretions (P<0.05). 6) Combined microbiome and metabolomics analysis showed that certain metabolites were significantly associated with microbial communities, especially Klebsiella. Conclusions: Significant differences in the microbiota genera and metabolites at different time points were found during the frozen-embryo transfer cycle of hormone replacement therapy, which may be used as potential biomarkers to predict pregnancy outcomes of embryo transfer.

Horizontal and Vertical Transmission of a Mycovirus Closely Related to the Partitivirus RhsV717 That Confers Hypovirulence in Rhizoctonia solani.

Rhizoctonia solani virus717 (RhsV717) was isolated from the Rhizoctonia solani (R. solani) AG-2 strain Rhs717. This study isolated a virus designated as Rhizoctonia solani partitivirus BS-5 (RsPV-BS5) from the R. solani AG-3 strain BS-5, the causal agent of tobacco target spot disease. The virus was identified as a strain of RhsV717. Transmission electron microscopy (TEM) images showed that RsPV-BS5 had virus particles with a diameter of approximately 40 nm. Importantly, it can be horizontally transmitted through hyphal anastomosis and vertically transmitted via sexual basidiospores. Furthermore, this study demonstrated that RsPV-BS5 infection significantly impedes mycelial growth and induces hypovirulence in tobacco leaves. Thus, RsPV-BS5 presents a promising avenue for biocontrolling tobacco target spot disease. Transcriptome analysis unveiled differential expression of four genes related to cell wall-degrading enzymes between two isogenic strains, 06-2-15V and 06-2-15. These findings shed light on the molecular mechanism through which RsPV-BS5 reduces host pathogenicity.

Exosomes: Mediators in microenvironment of colorectal cancer.

Tumor microenvironment, the soil where tumor thrives, plays a critical role in the development and progression of colorectal cancer (CRC). Various cell signaling molecules in the environment promote tumor angiogenesis, immune tolerance and facilitate immune escape. Exosomes, as messengers between tumor and host cells, are considered key mediators involved in the tumor-accelerating environment. However, the exosome-mediated communication networks in the CRC microenvironment are still largely unclear. In this review, we summarized the relationship between TME and CRC based on recent literature. Then, we revealed the unique impacts and signal molecules of exosomes on account of their regulatory role in the flora, hypoxia, inflammatory and immunological microenvironment of CRC. Finally, we summarized the therapeutically effective of exosomes in CRC microenvironment and discussed their current status and prospects, aiming to provide new molecular targets and a theoretical basis for the CRC treatment.

Perioperative Exercise Intention and Influencing Factors: A Multi-Centered Cross-Sectional Study.

Objectives: This study aimed to evaluate the level and factors affecting the perioperative exercise intention in China. Design: This study was a cross-sectional survey in Southwest China. Methods: Four hundred and ninety nine participants were randomly sampled in eight medical centers from November 23, 2020 to November 27, 2020. The survey included sociodemographic information and a 24-item modified questionnaire, which aimed to evaluate the attitude toward daily exercise, perception of perioperative exercise, social support and the perioperative exercise intention. A multivariable linear regression model was used to evaluate the effect of different items on the patients' intention for perioperative exercise. Results: A total of 523 responses (95.09%) were collected and 499 (95.41%) were analyzed. The level of exercise intention of the patients during the perioperative period was: 14.83% planned to exercise every day in the hospital, 21.04% planned to exercise every other day, and 35.87% planned to exercise every week. Intensity of daily exercise (P = 0.016), positive attitude of daily exercise (P < 0.001), positive attitude of perioperative exercise (P < 0.001) and social support (P < 0.001) were positively associated with the intention for perioperative exercise. Female (P = 0.012), non-tertiary center (P = 0.011), and preoperative anxiety (P = 0.023) was negatively associated with it. Conclusions: The intention for perioperative exercise was low in Southwest China. The authors aimed to relieve preoperative anxiety, promote the education of perioperative exercise, design perioperative exercise programs, and provide more social support from medical staff and family for inpatients undergoing elective surgery.

Biomimetic fabrication of new bioceramics-introduced fibrous scaffolds: From physicochemical characteristics to in vitro biological properties.

The combination of biodegradable polymers and bioactive inorganic materials is a promising method to mimic native tissue in bone regeneration. Toward this direction, electrospun fibrous scaffolds were successfully fabricated in the silk fibroin (SF) matrix containing new bioceramics on the basis of mesoporous bioactive glass/hydroxyapatite nanocomposite (MGHA). The physicochemical properties and surface hydrophilicity of these biphasic composite could be tailored by the addition of MGHA content. The increase in surface hydrophilicity and bioactivity of the as-spun composite fibers were observed with the increasing the nanoparticle contents while decreasing their tensile strength. In vitro cytotoxicity evaluation based on human bone marrow-derived mesenchymal stem cells (hMSCs) revealed that a positive osteogenic differentiation effect on SF/MGHA7 sample as evidenced by an increased alkaline phosphatase (ALP) activity, and upregulated osteoblastic gene expression compared with SF samples. These findings supported the suitability of the SF/MGHA composite system for its potential application in cell-material combination in bone tissue engineering.

[Value of 18F-FDG PET imaging in diagnosing tumor residue of intracranial glioma after surgery and radiotherapy].

BACKGROUND & OBJECTIVE: It is difficult to diagnose tumor residue by CT/MRI after treatment. The application of (18)F-fluorodeoxyglucose positron emission tomography ((18)F-FDG PET) to determine the suspected tumor residue after treatment has become a hot target in the study of radiotherapy. This study was designed to discuss the clinical value of (18)-FDG PET imaging in post-operative and post-radiotherapeutic intracranial glioma. METHODS: (18)F-FDG PET imaging was performed in 23 patients with post-operative and post-radio-therapeutic intracranial glioma, and compared with CT/MRI. The final diagnosis of tumor residue was proved by pathology or clinical follow-up. RESULTS: Of 23 patients, 12 showed (18)F-FDG PET positive, and 11 showed negative,among which 3 were false negative. The accuracy of (18)F-FDG PET was 87.0% (20/23), significantly higher than 60.9% (14/23) of CT/MRI scan (P< 0.05). The diagnosis of tumor residue in 9 patients cannot be determined by CT/MRI, while 4 of these patients showed (18)F-FDG PET positive, and the other 5 showed (18)F-FDG PET negative. Eight of 23 patients diagnosed tumor residues by CT/MRI, showed (18)F-FDG PET positive,too. Six patients,diagnosed by CT/MRI as radioactive-disease sufferers, and PET indicated with low or deficient FDG metabolism, were proved to have radioactive diseases by follow-up. CONCLUSIONS: (18)F-FDG PET imaging has significant dominance in characterizing lesions,and differentiating tumor residue in post-operative and post-radiotherapeutic intracranial glioma. Combined with CT and MRI, it can provide both anatomical and functional information for treatment.

[Detection of residue of nasopharyngeal carcinoma with FDG PET].

BACKGROUND AND OBJECTIVE: The use of F-18-fluoro-2-deoxy-d-glucose positron emission tomography (FDG PET) in detection of suspected residue of malignant tumor after treatment has become one of the most important targets of study in radiotherapy. This study was designed to evaluate FDG PET in detection of suspected residue of nasopharyngeal carcinoma (NPC) after treatment, in comparison with CT imaging. METHODS: Twenty-five patients with clinically suspected residue of NPC did FDG PET Scanning, 23 of them also examine with CT imaging. The diagnosis of residue were confirmed by pathology or clinical follow-up. RESULTS: FDG PET correctly detected residue in 19/25 patients. There were 4 false positive and 2 false negative patients. The accuracy of FDG PET was 76.0% (19/25), compared to 52.2% (12/23) of CT scan. Eleven of 23 patients did not show any residue by CT scan, while, FDG PET scan showed significantly increased FDG metabolism, indicating residue in 2 of these patients. In 7 patients, both CT and FDG PET indicated residue tumor. CONCLUSION: Compared with CT imaging, FDG PET has a higher accuracy in detection of residue nasopharyngeal carcinoma after treatment.