RESUMO
Commercial layer chicks of different ages were inoculated with either one-fifth, two-fifths or a full dose of an inactivated infectious bursal disease oil emulsion vaccine and then challenged with virulent virus. The partial doses given at seven or 10 days old gave only partial protection. A full dose given at 10, 14 or 28 days old failed to give full protection but a full dose administered at seven days old protected all the chicks after each challenge.
Assuntos
Galinhas , Vírus da Doença Infecciosa da Bursa/imunologia , Doenças das Aves Domésticas/prevenção & controle , Infecções por Reoviridae/veterinária , Vacinas Virais , Animais , Anticorpos Antivirais/sangue , Imunodifusão , Vírus da Doença Infecciosa da Bursa/patogenicidade , Distribuição Aleatória , Infecções por Reoviridae/prevenção & controle , Vacinação/veterinária , Vacinas de Produtos Inativados/administração & dosagem , Vacinas de Produtos Inativados/imunologia , Vacinas Virais/administração & dosagem , Vacinas Virais/imunologia , VirulênciaRESUMO
A comparison was made of the serum neutralisation, immunofluorescent antibody and immunoperoxidase tests for the detection of antibodies to chicken anaemia agent. Serum samples from groups of chicks with and without maternally derived antibody to the agent were tested and the response of chicks after inoculation with the agent was also measured. The serum neutralisation test was reliable and sensitive, but expensive and could take up to three weeks to obtain a result. The immunofluorescent antibody test was cheaper and required only one day to obtain a result, but it was not as sensitive in detecting low levels of antibody to the chicken anaemia agent. The immunoperoxidase test was also cheaper but took two days to obtain a result and required one more manipulation than the immunofluorescence test. It was comparable to the serum neutralisation test in its ability to detect low levels of antibody.
Assuntos
Anemia/veterinária , Anticorpos Antivirais/análise , Galinhas/imunologia , Parvoviridae/imunologia , Anemia/microbiologia , Animais , Imunofluorescência/veterinária , Técnicas Imunoenzimáticas/veterinária , Testes de Neutralização/veterináriaRESUMO
The morphological, bio-physical and growth properties of the isolate duck/Hong Kong/D3/75 (D3/75) were consistent with this virus being a member of the paramyxovirus group. Using haemagglutination inhibition and neuraminidase inhibition tests no serological relationships between D3/75 and other paramyxoviruses could be demonstrated. The structural polypeptides of D3/75 were also typical of paramyxoviruses, consisting of 6--7 polypeptides ranging in molecular weight from 46,000--200,000 under reduced conditions. Two polypeptides were glycosylated.