Fetuin as a potential serum biomarker to detect subclinical shedder of bovine paratuberculosis.

Paratuberculosis (PTB) is a chronic contagious granulomatous enteritis of wild and domestic ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). PTB causes considerable economic losses to the dairy industry through decreased milk production and premature culling. PTB-affected cattle undergo a subclinical stage without clinical signs and initiate fecal shedding of MAP into the environment. Current diagnostic tools have low sensitivity for the detection of subclinical PTB infection. Therefore, alternative diagnostic tools are required to improve the diagnostic sensitivity of subclinical PTB infection. In this study, we performed ELISA for three previously identified host biomarkers (fetuin, alpha-1-acid glycoprotein, and apolipoprotein) and analyzed their diagnostic performance with conventional PTB diagnostic methods. We observed that serum fetuin levels were significantly lowered in the subclinical shedder and clinical shedder groups than in the healthy control group, indicating its potential utility as a diagnostic biomarker for bovine PTB. Also, fetuin showed an excellent discriminatory power with an AUC = 0.949, a sensitivity of 92.6%, and a specificity of 94.4% for the detection of subclinical MAP infection. In conclusion, our results demonstrated that fetuin could be used as a diagnostic biomarker for enhancing the diagnostic sensitivity for the detection of subclinical MAP infections that are difficult to detect based on current diagnostic methods.

Alpha-2-Macroglobulin as a New Promising Biomarker Improving the Diagnostic Sensitivity of Bovine Paratuberculosis.

Johne's disease (JD) is a chronic granulomatous enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP), which induces persistent diarrhea and cachexia. JD causes huge economic losses to the dairy industry due to reduced milk production and premature culling. Infected animals excrete MAP via feces during the prolonged subclinical stage without exhibiting any clinical signs. Therefore, accurate detection of subclinical stage animals is crucial for successful eradication of JD in the herd. In the current study, we analyzed serum samples of MAP-infected and non-infected cattle to identify potential biomarker candidates. First, we identified 12 differentially expressed serum proteins in subclinical and clinical shedder groups compared to the healthy control group. Second, we conducted ELISA for three selected biomarkers (alpha-2-macroglobulin (A2M), alpha-1-beta glycoprotein, and transthyretin) and compared their diagnostic performance with that of two commercial ELISA diagnostic kits. Serum A2M levels were significantly higher in the MAP-exposed, subclinical shedder, subclinical non-shedder, and clinical shedder groups than in the healthy control group, suggesting its possible use as a diagnostic biomarker for MAP infection. Furthermore, A2M demonstrated a sensitivity of 90.4%, and a specificity of 100% while the two commercial ELISA kits demonstrated a sensitivity of 67.83 and 73.04% and a specificity of 100%, respectively. In conclusion, our results suggest that measuring A2M by ELISA can be used as a diagnostic tool to detect MAP infection, considerably improving the detection rate of subclinical shedders and MAP-exposed animals that are undetectable using current diagnostic tools.

Increased Risk of Severe Gastric Symptoms by Virulence Factors vacAs1c, alpA, babA2, and hopZ in Helicobacter pylori Infection.

Two virulence factors of Helicobacter pylori, cagA and vacA, have been known to play a role in the development of severe gastric symptoms. However, they are not always associated with peptic ulcer or gastric cancer. To predict the disease outcome more accurately, it is necessary to understand the risk of severe symptoms linked to other virulence factors. Several other virulence factors of H. pylori have also been reported to be associated with disease outcomes, although there are many controversial descriptions. H. pylori isolates from Koreans may be useful in evaluating the relevance of other virulence factors to clinical symptoms of gastric diseases because the majority of Koreans are infected by toxigenic strains of H. pylori bearing cagA and vacA. In this study, a total of 116 H. pylori strains from Korean patients with chronic gastritis, peptic ulcers, and gastric cancers were genotyped. The presence of virulence factors vacAs1c, alpA, babA2, hopZ, and the extremely strong vacuolating toxin was found to contribute significantly to the development of severe gastric symptoms. The genotype combination vacAs1c/alpA/babA2 was the most predictable determinant for the development of severe symptoms, and the presence of babA2 was found to be the most critical factor. This study provides important information on the virulence factors that contribute to the development of severe gastric symptoms and will assist in predicting clinical disease outcomes due to H. pylori infection.

Comparison of the Posterior Vertebral Column Resection With the Expandable Cage Versus the Nonexpandable Cage in Thoracolumbar Angular Kyphosis.

STUDY DESIGN: This was a retrospective study. OBJECTIVE: To compare the radiographic and clinical results of anterior support with nonexpandable cage with those of expandable cage. SUMMARY OF BACKGROUND DATA: The goals of surgical treatment in patients with thoracolumbar angular kyphosis are to improve the neurological deficit through neural decompression, to restore the normal alignment by correction of deformity, and to stabilize the spinal column by arthrodesis. Mesh cages have been traditionally used for reconstruction after corpectomy. However, expandable cages are gaining popularity due to some advantages over mesh cages, structural autograft or allograft, and poly-ether-ether-ketone/carbon fiber. MATERIALS AND METHODS: We performed a retrospective study in a consecutive series of 42 subjects with a male-to-female ratio of 15:27, a mean age of 65.3±10.9 years, and a mean follow-up period of 36.4±7.1 months, who underwent posterior vertebral column resection at our medical institutions between 2006 and 2011. They consist of 32 patients with posttraumatic kyphosis with a mean kyphotic angle of 33.1 degrees (range, 12.2-58.1 degrees) and 10 patients with tuberculous kyphosis with a mean kyphotic angle of 53.9 degrees (range, 22.6-126.0 degrees). The mean follow-up period was 36.4 months (range, 28-54 mo). We compared the clinical outcomes between the 2 groups based on radiographic findings, neurological status, and complications. Intervertebral heights and kyphosis at the level of vertebral column resection were used to measure the radiographic outcomes. Neurological outcome was evaluated using the modified Frankel grading system. RESULTS: Postoperatively, the mean kyphosis angle was 3.0±10.8 and 6.7±7.2 degrees (P=0.094). At a final follow-up, these values were 4.7±10.1 and 12.3±18.9 degrees in the corresponding order (P=0.099). There were 19 (80.0%) and 21 (86.4%) patients with solid arthrodesis of grade 1. On modified Frankel grading system, all the patients but one achieved improvement. But there was no significant difference in the grade between the 2 groups (P>0.05). Also in our series, there were 12 patients with cage subsidence and 6 with screw loosening. CONCLUSIONS: Nonexpandable cage and expandable cage are viable options for anterior support; both cages allow for correction of kyphosis with good fusion rate with similar neurological outcomes. Expandable cage is effective for the anterior support of posterior vertebral column resection.