Radiotracer studies to isolate in-house receptors from poultry liver for multi-chemical hazard analysis in selected food and feed.

In-house receptors (IHRs) were isolated from non-immunized poultry liver to analyze selected contaminants and residues in targeted food and feed using 14C- and 3H-labeled radiotracers. Matrix (2 g) was homogenized and centrifuged with the resultant pellet used as IHRs. These were characterized for total protein contents (6.1 mg mL-1) and compared with commercial receptors for aflatoxins (0.28 mg tablet-1) and chloramphenicol (0.12 mg tablet-1). Gel electrophoresis of the IHRs showed a mixture of polypeptides-an important attribute for multi-residues analysis-compared with commercial receptors that presented specific protein bands at 65 kDa (chloramphenicol) and 70 kDa (aflatoxins). The inhibition index of IHRs for aflatoxins B1 and B2 in wheat and bovine feed and chloramphenicol in bovine tissue at, above, and below maximum limits or minimum required performance limits, revealed an inverse relationship between radiotracer and analyte concentrations. Saturation with increased radioligand concentration up to 5.5 kBq indicated higher holding potential. However, increasing incubation time to 30 min did not significantly increase analyte-binding. The IHRs performance was comparable to commercial receptors with control point averages of 348, 410, 555, and 307 counts per minute determined for gentamicin, chloramphenicol, oxytetracycline, and aflatoxin M1, respectively in local milk samples.

The Role of Polyphenols in Regulation of Heat Shock Proteins and Gut Microbiota in Weaning Stress.

Gut microbiota is the natural residents of the intestinal ecosystem which display multiple functions that provide beneficial effects on host physiology. Disturbances in gut microbiota in weaning stress are regulated by the immune system and oxidative stress-related protein pathways. Weaning stress also alters gut microbiota response, limits digestibility, and influences animal productive performance through the production of inflammatory molecules. Heat shock proteins are the molecular chaperones that perform array functions from physiological to pathological point of view and remodeling cellular stress response. As it is involved in the defense mechanism, polyphenols ensure cellular tolerance against enormous stimuli. Polyphenols are nature-blessed compounds that show their existence in plenty of amounts. Due to their wider availability and popularity, they can exert strong immunomodulatory, antioxidative, and anti-inflammatory activities. Their promising health-promoting effects have been demonstrated in different cellular and animal studies. Dietary interventions with polyphenols may alter the gut microbiome response and attenuate the weaning stress related to inflammation. Further, polyphenols elicit health-favored effects through ameliorating inflammatory processes to improve digestibility and thereby exert a protective effect on animal production. Here, in this article, we will expand the role of dietary polyphenol intervention strategies in weaning stress which perturbs gut microbiota function and also paid emphasis to heat shock proteins in gut health. This review article gives new direction to the feed industry to formulate diet containing polyphenols which would have a significant impact on animal health.

Role of Dietary Amino Acids and Nutrient Sensing System in Pregnancy Associated Disorders.

Defective implantation is related to pregnancy-associated disorders such as spontaneous miscarriage, intrauterine fetal growth restriction and others. Several factors proclaimed to be involved such as physiological, nutritional, environmental and managemental that leads to cause oxidative stress. Overloading of free radicals promotes oxidative stress, and the internal body system could not combat its ability to encounter the damaging effects and subsequently leading to pregnancy-related disorders. During pregnancy, essential amino acids display important role for optimum fetal growth and other necessary functions for continuing fruitful pregnancy. In this context, dietary amino acids have received much attention regarding the nutritional concerns during pregnancy. Arginine, glutamine, tryptophan and taurine play a crucial role in fetal growth, development and survival while ornithine and proline are important players for the regulation of gene expression, protein synthesis and angiogenesis. Moreover, amino acids also stimulate the mammalian target of rapamycin (mTOR) signaling pathway which plays a central role in the synthesis of proteins in placenta, uterus and fetus. This review article explores the significances of dietary amino acids in pregnancy development, regulation of nutrient-sensing pathways such as mTOR, peroxisome proliferator-activated receptors (PPARs), insulin/insulin-like growth factor signaling pathway (IIS) and 5' adenosine monophosphate-activated protein kinase (AMPK) which exhibit important role in reproduction and its related problems. In addition, the antioxidant function of dietary amino acids against oxidative stress triggering pregnancy disorders and their possible outcomes will also be enlightened. Dietary supplementation of amino acids during pregnancy could help mitigate reproductive disorders and thereby improving fertility in animals as well as humans.