Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros











Base de dados
Intervalo de ano de publicação
1.
J Vet Diagn Invest ; 36(1): 70-77, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-38014733

RESUMO

We assessed the effects of fixation time in formalin and inclusion of surrounding tissue on microRNA (miRNA) cycle quantification (Cq) values in formalin-fixed, paraffin-embedded (FFPE) urothelial carcinoma (UC) tissue (n = 3), and the effect of conditions on miRNAs in urine from 1 healthy dog. MiRNAs were extracted using commercial kits and quantified using miRNA-specific fluorometry in normal bladder tissue scrolls, UC tissue cores, and bladder muscularis tissue cores from 4 FFPE bladder sections (3 UCs, 1 normal), plus 1 UC stored in formalin for 1, 8, 15, and 22 d before paraffin-embedding. Urine was collected from a healthy dog on 4 occasions; 1-mL aliquots were stored at 20, 4, -20, and -80°C for 4, 8, 24, and 48 h, and 1 and 2 wk. For both FFPE tissue and urine, we used reverse-transcription quantitative real-time PCR (RT-qPCR) to quantify miR-143, miR-152, miR-181a, miR-214, miR-1842, and RNU6B in each tissue or sample, using miR-39 as an exogenous control gene. The Cq values were compared with ANOVA and t-tests. The time of tissue-fixation in formalin did not alter miRNA Cq values; inclusion of the muscularis layer resulted in a statistically different miRNA Cq profile for miR-152, miR-181a, and RNU6B in bladder tissue. MiRNAs in acellular urine were stable for up to 2 wk regardless of the storage temperature. Our findings support using stored FFPE and urine samples for miRNA detection; we recommend measuring miRNA only in the tissue of interest in FFPE sections.


Assuntos
Carcinoma de Células de Transição , Doenças do Cão , MicroRNAs , Neoplasias da Bexiga Urinária , Cães , Animais , MicroRNAs/genética , MicroRNAs/análise , Projetos Piloto , Carcinoma de Células de Transição/genética , Carcinoma de Células de Transição/veterinária , Neoplasias da Bexiga Urinária/veterinária , Inclusão em Parafina/veterinária , Formaldeído , Fixação de Tecidos/veterinária , Fixação de Tecidos/métodos , Doenças do Cão/diagnóstico , Doenças do Cão/genética , Doenças do Cão/patologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA